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Plankton community composition in the Three Gorges Reservoir Region revealed by PCR-DGGE and its relationships with environmental factors

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Journal of Environmental Sciences 2008年第6期20卷 732-738页

作者： YAN Qingyun yu yuhe FENG Weisong yu Zhigang CHEN Hongtao Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China Ocean University of China Qingdao 266003 China

To explore the relationships between community composition and the environment in a reservoir ecosystem, plankton communities from the Three Gorges Reservoir Region were studied by PCR-denaturing gradient gel electrophoresis fingerprinting. Bacterial and eukaryotic operational taxonomic units （OTUs）, generated by DGGE analysis of the PCR-amplified 16S and 18S rRNA genes, were used as surrogates for the dominant ＂biodiversity units＂. OTU composition among the sites was heterogeneous; 46.7% of the total bacterial OTUs （45） and 64.1% of the eukaryotic OTUs （39） were identified in less than half of the sampling sites. Unweighted pair group method with arithmetic averages （UPGMA） clustering of the OTUs suggested that the plankton communities in the Xiangxi Rive sites were not always significantly different from those from the Yangtze River sites, despite clear differences in their environmental characterizations. Canonical correspondence analysis （CCA） was applied to further investigate the relationships between OTU composition and the environmental factors. The first two CCA ordination axes suggested that the bacterial community composition was primarily correlated with the variables of NO3^--N, dissolved oxygen （DO）, and SiO3^2--Si, whereas, the eukaryotic community was mainly correlated with the concentrations of DO, PO4^3--P, and SiO3^2--Si.

关键词： plankton community environmental factors denaturing gradient gel electrophoresis (DGGE) spatial pattern Three Gorges Reservoir Region

Comparative study on the gut microbiotas of four economically important Asian carp species

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Science China(Life Sciences) 2018年第6期61卷 696-705页

作者： Xinghao Li yuhe yu Chang Li Qingyun Yan Laboratory of Protozoology Institute of Evolution and Marine Biodiversity OUC Qingdao 266003 China Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences and State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of Sciences Wuhan 430072 China Environmental Microbiomics Research Center School of Environmental Science and Engineering Sun Yat-sen University Guangzhou 510006 China

Gut microbiota of four economically important Asian carp species(silver carp, Hypophthalmichthys molitrix; bighead carp,Hypophthalmichthys nobilis; grass carp, Ctenopharyngodon idella; common carp, Cyprinus carpio) were compared using 16 S rRNA gene pyrosequencing. Analysis of more than 590,000 quality-filtered sequences obtained from the foregut, midgut and hindgut of these four carp species revealed high microbial diversity among the samples. The foregut samples of grass carp exhibited more than 1,600 operational taxonomy units(OTUs) and the highest alpha-diversity index, followed by the silver carp foregut and midgut. Proteobacteria, Firmicutes, Bacteroidetes and Fusobacteria were the predominant phyla regardless of fish species or gut type. Pairwise(weighted) UniFrac distance-based permutational multivariate analysis of variance with fish species as a factor produced significant association(P<0.01). The gut microbiotas of all four carp species harbored saccharolytic or proteolytic microbes, likely in response to the differences in their feeding habits. In addition, extensive variations were also observed even within the same fish species. Our results indicate that the gut microbiotas of Asian carp depend on the exact species, even when the different species were cohabiting in the same environment. This study provides some new insights into developing commercial fish feeds and improving existing aquaculture strategies.

关键词： Asian carp gut microbiota feeding habit pyrosequencing Hypophthalmichthys molitrix Hypophthalmichthys nobilis Ctenopharyngodon idella Cyprinus carpio

Impacts of algal blooms removal by chitosan-modified soils on zooplankton community in Taihu Lake,China

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Journal of Environmental Sciences 2010年第10期22卷 1500-1507页

作者： Jiajia Ni yuhe yu Weisong Feng Qingyun Yan Gang Pan Bo Yang Xiang Zhang Xuemei Li Key Laboratory of Biodiversity and Conservation of Aquatic Organisms Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China. Graduate School of the Chinese Academy of Sciences Beijing 100039 China State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-environmental Sciences Chinese Academy of Sciences Beijing 100085 China

It is important to assess the effect on zooplankton when perform the environmental protection or restoration technology, especially removing algal blooms, because algae were the major primary producer in algal lakes. The influence on zooplankton community after half a year of algal blooms removed by chitosan-modified soils in Taihu Lake was assessed and the rationality of carrying out the process semiannually was evaluated in the present study. Morphological composition and genetic diversity of zooplankton community were investigated by microscope checkup and polymerase chain reaction-denatured gradient gel electrophoresis （PCR-DGGE）. A total of 44 zooplankton taxa （23 protozoa, 17 rotifers, 3 copepoda and 1 cladocera） were detected by microscope checkup, and a total of 91 bands （28 bands amplified by primers F1427-GC and R1616, 63 bands amplified by primers Fung-GC and NS1） were detected by PCR-DGGE. The results of cluster analysis or detrended correspondence analysis indicated that there was no considerable difference in morphological composition of zooplankton and DGGE profiles between experimental and control sites, and DGGE profiles could represent the biologic diversity. The study showed that zooplankton community could recover original condition after half year of algal blooms removed by chitosan-modified soils and it was acceptable to apply this process semiannually. In addition, the results revealed that PCR-DCJGE could be applied to investigate the impacts of the environmental protection or restoration engineering on zooplankton community diversity.

关键词： algal blooms Microcystis aeruginosa zooplankton community chitosan-modified soils Taihu Lake

Spatio-temporal patterns of zooplankton in a main-stem dam affected tributary: a case study in the Xiangxi River of the Three Gorges Reservoir, China

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Science China(Life Sciences) 2019年第8期62卷 1058-1069页

作者： Xinghao Li Jie Huang Sabine Filker Thorsten Stoeck Yonghong Bi yuhe yu Weibo Song Laboratory of Protozoology Institute of Evolution and Marine BiodiversityOcean University of ChinaQingdao266003China Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences and State Key Laboratory of Freshwater Ecologyand BiotechnologyInstitute of HydrobiologyChinese Academy of SciencesWuhan430072China Department of Molecular Ecology University of Technology KaiserslauternKaiserslautern67663Germany Department of Ecology University of Technology KaiserslauternKaiserslautern67663Germany Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao266003China

As the ecologically important recipient channels for riverine ecosystems, tributaries provide unique microhabitats for microorganisms, among which zooplankton constitutes the most important heterotrophic organisms. In particular, the reduced water velocity caused by dams is more favorable for zooplankton development;therefore, dammed rivers are expected to support extremely diverse and abundant zooplankton communities and notably different spatiotemporal distribution patterns. So far,however, only very few molecular studies support these assumptions. Using high-throughput sequencing, a high number of 350 operational taxonomic units(OTUs;97% cutoff) were retrieved from 30 samples collected in the Xiangxi River, the nearest large tributary upstream of the Three Gorges Dam. Zooplankton did not show significant spatial distribution in the channel. Instead,the community structures varied significantly over sampling dates, corroborating the seasonal patterns found in lakes and ponds in the subtropical zone. As expected, the community compositions were deterministically governed by environmental filtering processes(phylogenetic clustering), in which water velocity appeared to be much less important than other investigated environmental factors. Moreover, most of the detected phylotypes(OTUs) had a relatively high(>90%) sequence similarity to previously deposited sequences, suggesting a mediocre degree of genetic novelty within the zooplankton communities in the Xiangxi River.

关键词： zooplankton high-throughput sequencing spatiotemporal distribution genetic novelty tributary main-stem dam

Genetic fingerprinting of plankton community provides new insights into aquatic ecology

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Progress in Natural Science:Materials International 2006年第9期16卷 893-898页

作者： YAN Qingyun, yu yuhe and FENG Weisong ( Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China Graduate School of the Chinese Academy of Sci-ences, Beijing 100039, China) [a]Institute of Hydrobiology Chinese Academy of Sciences Wuhan China [b]Graduate School of the Chinese Academy of Sciences Bejing China

Over the past two decades, molecular techniques have been widely used in ecological study and molecular ecology has been one of the most important branches of ecology. Meanwhile, genetic fingerprinting analyses have significantly enhanced our knowledge of the diversity and evolutionary relations of the planktonic organisms. Compared with conventional approaches in ecological study (e.g. morphological classification), genetic fingerprinting techniques are simpler and much more effective. This review provides an overview of the principles, advantages and limitations of the commonly used DNA fingerprinting techniques in plankton research. The aim of this overview is to assess where we have been, where we are now and what the future holds for solving aquatic ecological problems with molecular-level information.

关键词： genetic fingerprinting plankton community aquatic ecology genetic diversity.

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村民自治视域下的乡村德治论纲

山东大学学报（哲学社会科学版） 2020年第1期 134-142页

作者：于语和雷园园南开大学天津300350 南开大学马克思主义学院天津300350

乡村振兴要植根于中国乡村的社会和文化土壤之中,植根于乡村土地上努力生活的具体的人,植根于多样性的乡村特色和集体记忆之中。立足乡村发展和村民的现实需要,回溯传统社会的德治传统,激活并超越性借鉴中国古代思想精华,乡村德治才能... 详细信息

乡村振兴要植根于中国乡村的社会和文化土壤之中,植根于乡村土地上努力生活的具体的人,植根于多样性的乡村特色和集体记忆之中。立足乡村发展和村民的现实需要,回溯传统社会的德治传统,激活并超越性借鉴中国古代思想精华,乡村德治才能真正为盘活村民自治提供契机和动力。在新的历史条件下,伴随民族国家的建设进程和乡村治理体系的推进,乡村德治主体趋向多元化,并依据法理化的民间规范和礼俗化的国家价值观重构起多维协同共治的权威、话语体系。其中,村规民约中的柔性线条和家风家法的核心内容充分彰显现代德治的新特质,乡贤治村和人民调解中日益凸显的德治功能成为村民自治视域下规范村民行为、化解社会冲突的合理依据。

关键词：乡村德治德治模式民间调解村规民约乡贤治村

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《法经》与《十二铜表法》之比较研究

南开学报（哲学社会科学版） 2000年第4期 90-96页

作者：于语和董跃天津大学社科学院天津300072

《法经》和《十二铜表法》分别被认为是中华法系和罗马法系的起源和基础。两者产生于大致相同的年代，都具有古代法律文化的一般性特征。又因各自背景、渊源不同，在立法倾向、对待和法关系的态度及蕴含的法治精神上存在着明显差异。... 详细信息

《法经》和《十二铜表法》分别被认为是中华法系和罗马法系的起源和基础。两者产生于大致相同的年代，都具有古代法律文化的一般性特征。又因各自背景、渊源不同，在立法倾向、对待和法关系的态度及蕴含的法治精神上存在着明显差异。通过对两者的比较可以发现，中国法律文化和西方法律文化，早在《法经》和《十二铜表法》时代就已经埋下分歧的种子，走上了不同的发展道路。

关键词： > > 比较研究法治精神

试论民间习惯在民间纠纷调解中的作用——以河北省某村的实地调查为个案

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法学家 2005年第3期 55-62页

作者：于语和于浩龙南开大学法政学院

习惯在当代中国社会的纠纷解决中起着重要作用,在很多情况下,人们遵从习惯多于遵从法律。本文以个案为角度,探讨了在民间纠纷调解中民间习惯的作用以及这种作用的发生机制。从调查的案例得出结论,习惯的最大作用在于受到广泛的认同,通... 详细信息

习惯在当代中国社会的纠纷解决中起着重要作用,在很多情况下,人们遵从习惯多于遵从法律。本文以个案为角度,探讨了在民间纠纷调解中民间习惯的作用以及这种作用的发生机制。从调查的案例得出结论,习惯的最大作用在于受到广泛的认同,通过习惯解决纠纷有利于执行。文章结论在我国法治建设中,应当注重发挥习惯的作用,应当承认习惯的效力。

关键词：民间习惯民间纠纷调解制度制定法中国

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先秦儒家性善论思想的法治意蕴探究

西北民族大学学报（哲学社会科学版） 2021年第1期 99-105页

作者：于语和雷园园南开大学法学院天津300350 南开大学马克思主义学院天津300350

先秦时期,儒墨道法各家学派竞相争鸣,深刻影响了中华民族法律文化的形成基因和未来走向,以法治理念审视中国文化元素,不难发现法治中国的理性建构思路深受隐含于内的性善论思想的观照,推动法治由一般意义的规则之治走向具有品质和德性... 详细信息

先秦时期,儒墨道法各家学派竞相争鸣,深刻影响了中华民族法律文化的形成基因和未来走向,以法治理念审视中国文化元素,不难发现法治中国的理性建构思路深受隐含于内的性善论思想的观照,推动法治由一般意义的规则之治走向具有品质和德性的规则之治。先秦儒家的性善论思想是人性善思想的理论源头,形塑了中华民族的知识结构、制度规范和生活逻辑,是培育中国特色社会主义法治理论最深厚的沃土,更是建设法治中国的内在根基。人性善的理论预设奠定了"以人为首"的法哲学观,讲求"惟齐非齐""圆融人情"的合理正义,是新时代全面依法治国思想必不可少的条件。从先秦性善论角度剖析全面依法治国思想既有助于推动中国法治实践的理论创新,又继承与弘扬了中国传统法律文化精髓,彰显新时代建设中国特色社会主义法治国家的制度自信、理论自信、文化自信。

关键词：性善论人本主义良法善治公平正义法治资源