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Effects of UV-A exposures on longevity and reproduction in Helicoverpa armigera, and on the development of its F1 generation

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Insect Science 2011年第6期18卷 697-702页

作者： Chang-yu Zhang Jian-yu Meng xiao-ping Wang Fen Zhu Chao-Liang Lei Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory College of Plant Science and TechnologyHuazhong Agricultural University Wuhan China

Helicoverpa armigera adults display a conspicuous positive phototactic behavior to light stimuli, and are especially sensitive to ultraviolet （UV） light. The effects of UV-A （longwave） exposures on adult longevity and reproduction in H. armigera were investigated, as well as the development of the Ft generation. Paired adults were exposed to UV-A for various time periods （0, 1, 5 and 9 h/day）, until the end of adult life. The results showed that adult longevity decreased with increasing exposure time for both sexes, and a significant decrease was observed after exposure for 5 and 9 h/day. Fecundity increased when adults were exposed for 1 and 5 h/day, and a significant difference was observed in the 5 h/day group. Oviposition rates of females in all treatments were significantly higher than in the control. Exposure to UV-A for longer periods （5 and 9 h/day） caused a decline in cumulative survival of F1 immature stages, but no significant differences were found in egg hatch, pupation and eclosion. The developmental periods of F1 larvae were significantly prolonged after exposure to UV-A for 5 and 9 h/day. UV-A radiation had no significant effects on F1 pupal period.

关键词： development Helicoverpa armigera longevity oxidative stress,reproduction UV-A

High Temperature Effects on Yeast-like Endosymbiotes and Pesticide Resistance of the Small Brown Planthopper, Laodelphax striatellus

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Rice science 2008年第4期15卷 326-330页

作者： ZHANG xiao-jie yu xiao-ping CHEN Jian-ming Institute of Plant Protection and Microbiology Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China College of Life and Environment Science Hangzhou Normal University Hangzhou 310036 China

The newly-hatched nymphs of the small brown planthopper (SBPH), Laodelphax striatellus, including field and sensitive populations, were subjected to the high-temperature (35°C) treatment. The number of yeast-like endosymbiotes in SBPH reduced by 23.47%–34.23%, 57.86%–61.51% and 88.96%–90.71% after the high-temperature treatment for 1 d, 2 d, and 3 d, respectively. However, the size of yeast-like endosymbiotes was not obviously affected. Resistance of SBPH to three insecticides (imidacloprid, chlorpyrifos and fipronil) decreased with the increase of treatment time.

关键词： Laodelphax striatellus yeast-like endosymbiote high temperature pesticide resistance insect pest

Tryptophan Modification and Fluorescence Spectrum of Hyaluronidase

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Chinese Chemical Letters 2005年第11期16卷 1492-1494页

作者： Li Rong TENG yu Zhuo CHU xiao ping ZHANG xiao Kun yu Jing WANG Song HAN Bo Xun ZHANG Hao FAN Lan Ying LIU College of Life Science Jilin University Changchun 130023

Tryptophan residues in hyaluronidase （HAase） were modified by N-bromosuccinimide （NBS）, the results indicated that there were eleven tryptophan residues in HAase, one of which was exposed and essential for the activity of the enzyme. The study on fluorescence quenching showed that KI could not quench all of the fluorescence from Trp residues in HAase, while acrylamide （Acr） could quench almost all of the fluorescence from Trp residues in HAase. The collisional quenching constants （KD） of HAase at different concentrations of Acr were calculated in terms of Stern-Volmer equation. The results implied that some of the Trp residues were buried in the interior of HAase and the Trp residue on the surface of HAase was not located in the hydrophobic pocket.

关键词： Hyaluronidase (HAase) tryptophan (Trp) fluorescence spectrum.

Simultaneous Stabilization for a Family of Plants

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Journal of Mathematical Research and Exposition 2008年第3期28卷 529-534页

作者： LU yu Feng XU xiao ping Department of Applied Mathematics Dalian University of Technology Liaoning 116024 China

In this paper, we discuss the problem of simultaneous stabilization for plants more than three by using Youla parametrization and give a necessary and sufficient condition for simultaneous stabilization.

关键词： complete nest nest algebra linear system stabilizable.

Loss of heterozygosity and microsatellite instabilities of fragile histidine triad gene in gastric carcinoma

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World Journal of Gastroenterology 2006年第23期12卷 3766-3769页

作者： yu-ping xiao Dong-Ying Wu Lei Xu Yan Xin Cancer Insititute No.1 Hospital of China Medical University Shenyang 110001Liaoning Province China Fourth Laboratory of Cancer Institute No.1 HospitalChina Medical University Shenyang 110001 Liaoning ProvinceChina

AIM： To detect the loss of heterozygosity （LOH） and microsatellite instabilities （MSI） of fragile histidine triad （FHIT） gene in gastric carcinoma and to study their association with the clinical pathological characteristics of gastric carcinoma. METHODS： LOH and MSI of FHIT gene were detected at four microsaterllite loci D3SI3H, D3S4103, D3SI481 and D3S1234 using PCR in matched normal and cancerous tissues from 50 patients with primary gastric cancer. RESULTS： The average frequency of LOH and MSI of FHIT gene in gastric cancer was 32.4% and 26.4% respectively. LOH and MSI of FHIT gene in gastric cancer had no association with histological, Borrmann, and Lauren＇s classification. LOH of FHIT gene in gastric cancer was related to invasive depth. The frequency of FHIT LOH in gastric cancer with serosa-penetration was obviously higher than that in gastric cancer without serosa-penetration （73.5% vs 37.5%, P 〈 0.05）. MSI of FHIT gene in gastric cancer was associated with the lymph node metastasis. The frequency of MSI in gastric cancer without lymph node metastasis was significantly higher than that in gastric cancer with lymph node metastasis （66.7% vs 34.3%, P 〈 0.05）. CONCLUSION： LOH of FHIT gene is correlated with invasive depth of gastric carcinoma. MSI of FHIT gene is correlated with lymph node metastases. LOH and MSI of FHIT gene play an important role in carcinogenesis of gastric cancer.

关键词： Gastric cancer FHIT gene Microsatellite instabilities

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手机通话网络度特征分析

物理学报 2013年第20期62卷 542-552页

作者：余晓平裴韬新疆石河子大学信息科学与技术学院石河子832003 中国科学院地理科学与资源研究所资源与环境信息系统国家重点实验室北京100101

手机通信数据详细记录了人们的通信行为,成为研究人们社会关系、行为模式的重要资源.通话号码个数、通话次数和时长是手机通信网络的基本属性.本文在复杂网络理论基础上,应用统计的方法研究了中国西部某城市三百余万手机用户不同节假日... 详细信息

手机通信数据详细记录了人们的通信行为,成为研究人们社会关系、行为模式的重要资源.通话号码个数、通话次数和时长是手机通信网络的基本属性.本文在复杂网络理论基础上,应用统计的方法研究了中国西部某城市三百余万手机用户不同节假日和工作日的四天通话数据在不同尺度下的号码度、通话度、时长度的分布以及平均号码度、平均通话度、平均时长度的特征.研究表明,所有尺度下,号码度、通话度、时长度均为幂律分布,幂指数随尺度、日期和指标的不同而不同,在[1.3,4]范围内波动.总体上,号码度幂指数大于通话度和时长度幂指数,入度幂指数大于出度幂指数;节假日幂指数大于相应指标的工作日幂指数,休息时段幂指数大于工作时段幂指数;与工作日相比,节假日的平均号码度和平均通话度较小,平均时长度较大.揭示了绝大多数用户每日只接打1个号码的电话,节假日期间接打电话的用户数、次数、时长减少,但平均通话时长增大的特征.

关键词：手机通话网络复杂网络度分布通话模式

高效液相色谱法测定林可霉素利多卡因凝胶中盐酸利多卡因的含量

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中国医院药学杂志 2005年第8期25卷 723-724页

作者：余小平衢州市药品检验所浙江衢州324002

目的:采用高效液相色谱法测定林可霉素利多卡因凝胶中盐酸利多卡因的含量。方法:采用HypersilC18色谱柱(200mm×4.6mm,5μm),以甲醇0.25mol·L1醋酸钠溶液乙腈(55∶26∶19)为流动相,流速1.0mL·min1,紫外检测波长:230nm。外标法峰面积... 详细信息

目的:采用高效液相色谱法测定林可霉素利多卡因凝胶中盐酸利多卡因的含量。方法:采用HypersilC18色谱柱(200mm×4.6mm,5μm),以甲醇0.25mol·L1醋酸钠溶液乙腈(55∶26∶19)为流动相,流速1.0mL·min1,紫外检测波长:230nm。外标法峰面积定量。结果:样品与凝胶基质分离良好,线性范围为59.41～138.63mg·L1,样品溶液在24h内稳定,平均加样回收率为99.7%,RSD为0.19%(n=9)。结论:本法简便、快速,结果准确、可靠,重现性好。

关键词：林可霉素利多卡因盐酸利多卡因凝胶高效液相色谱法

Effect of Nitrogen Fertilizer on Herbivores and Its Stimulation to Major Insect Pests in Rice

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Rice science 2007年第1期14卷 56-66页

作者： LU Zhong-xian yu xiao-ping Kong-luen HEONG HU Cui Institute of Plant Protection and Microbiology Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China International Rice Research Institute DAPO Box 7777 Metro Manila Philippine Institute of Applied Entomology Zhejiang University Hangzhou 310029 China

Nitrogen is one of the most important factors in development of herbivore populations. The application of nitrogen fertilizer in plants can normally increase herbivore feeding preference, food consumption, survival, growth, reproduction, and population density, except few examples that nitrogen fertilizer reduces the herbivore performances. In most of the rice growing areas in Asia, the great increases in populations of major insect pests of rice, including planthoppers （Nilaparvata lugens and Sogatella furcifera）, leaffolder （ Cnaphalocrocis rnedinalis）, and stem borers （Scirpophaga incertulas, Chilo suppressalis, S. innotata, C. polychrysus and Sesarnia inferens） were closely related to the long-term excessive application of nitrogen fertilizers. The optimal regime of nitrogen fertilizer in irrigated paddy fields is proposed to improve the fertilizer-nitrogen use efficiency and reduce the environmental pollution.

关键词： nitrogen fertilizer herbivore insect pests rice fertilizer-nitrogen use efficiency

高效液相色谱法测定苯甲酸苄酯乳膏中苯甲酸苄酯的含量

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中国医院药学杂志 2006年第10期26卷 1246-1247页

作者：余小平衢州市药品检验所浙江衢州324002

目的：建立高效液相色谱法测定苯甲酸苄酯乳膏中苯甲酸苄酯的含量。方法：采用岛津C18色谱柱（4．6mm×150mm，5μm），以甲醇-水-乙腈（70：20：10，v／v）为流动相，流速1．2mL·min。紫外检测器于230nm测定。结果：线性范围13．186-9... 详细信息

目的：建立高效液相色谱法测定苯甲酸苄酯乳膏中苯甲酸苄酯的含量。方法：采用岛津C18色谱柱（4．6mm×150mm，5μm），以甲醇-水-乙腈（70：20：10，v／v）为流动相，流速1．2mL·min。紫外检测器于230nm测定。结果：线性范围13．186-92．302mg·L^-1 r=1．000，样品溶液在24h内稳定，平均回收率分别为100．2％。RSD为0．44％（n=9）。结论：本法简便、快速，结果准确、可靠，重复性好。

关键词：高效液相色谱法苯甲酸苄酯乳膏苯甲酸苄酯含量测定