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Foxg1 mRNA overexpression in neonatal rats following hypoxic brain injury

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Neural Regeneration Research 2011年第1期6卷 41-46页

作者： Luquan Li Yi Zheng Guoliang Mo Fang Li jialin yu Neonatal Diagnosis and Treatment Center Children's Hospital of Chongqing Medical University Chongqing 400014 China

Forkhead box G1 （Foxgl） is expressed during the embryonic stage and in postnatal brain regions sensitive to hypoxia/ischemia injury, such as the hippocampus and cerebral cortex. To date, very little is known about Foxgl expression changes in the brain following hypoxia injury （HI）. The present study measured Foxgl mRNA expression using reverse-transcription polymerase chain reaction on days 3, 7, 14, 28, and 56 following HI to determine self-restorative features in the injured brain. In addition, mRNA expression of other related layer markers, such as Reelin, RORB, Foxpl, Foxp2, ER81, and Otx-1, was detected following HI. Results revealed significantly decreased Foxgl mRNA expression at 3 days after HI, which significantly increased by 56 days. Reelin and Foxp2 mRNA expression were upregulated until 56 days after HI, but Foxpl and ER81 mRNA expression decreased from day 14 to 56 following HI. In addition, Otx-1 and RORI3 mRNA expression decreased from day 3 to 28 after HI. These findings revealed Fxogl mRNA overexpression and varying degrees of restoration in the neonatal rat brain following HI.

关键词： Foxgl mRNA hypoxia injury neonatal rats cerebral cortex

The pathogenicity on legumes of Cucumber mosaic virus was determined by 243 nucleotides on 2a polymerase gene of viral RNA2

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Chinese Science Bulletin 2002年第9期47卷 748-750,794页

作者： TAO Xiaorong ZHOU Xueping LI Guixin yu jialin Institute of Biotechnology Zhejiang UniversityHangzhou 310029China National Key Laboratory on Agrobiotechnology China Agriculture UniversityBeijing 100094China

We had isolated and identified two Cucumber mosaic virus (CMV) isolates, the CMV red bean (CMV-RB) isolate and the CMV pea (CMV-P1) isolate. CMV-RB induces necrotic local lesions on inoculated leaves of broad bean, pea, cowpea and bean, and could not infect these hosts systemically. However, CMV-P1 was able to infect these legumes systemically. To study the difference of pathogenic-ity on the legumes induced by these two CMV isolates, the full-length infectious cDNA clones of CMV-Fny, which induced similar symptoms as CMV-RB in the four legumes, were used. The 243 nucleotides fragment, which encodes highly conserved GDD amino acid motif on 2a replicase gene of CMV-Fny RNA2, was replaced with that of CMV-P1. The constructed chimeric virus FP could infect these legumes systemically. The exchange of this region changes the virus symptoms on the legumes, indicating that this 243 nucleotides fragment has major effect on pathogenicity of CMV on the legumes.

关键词： Cucumber mosaic virus legumes replicase gene patho-genicity.

Over-expression of 72 ku protein of wheat yellow mosaic virus in E. coli and preparation of its antiserum

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Chinese Science Bulletin 2000年第6期45卷 525-528页

作者： Yiming Xing Ning Su Dawei Li jialin yu Yi Liu China Agr Univ State Key Lab Agrobiotechnol Beijing 100094 Peoples R China

By reverse transcription-polymerase chain reaction (RT-PCR), cDNA fragment of wheat yellow mosaic vir黶 (WYMV) RNA2 encoding 72 ku protein has been synthesized and cloned into plasmid pET30a(+) for overexpression in prokaryotic celis. BL21(DE3) pLys S of *** transformed with the recombinant plasmid pETP72 containing the fragment has been induced to express the 72 ku protein on high level. The produced protein has been purified from sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) for its antiserum preparation. in western-blotting analysis, the antibodies reacted with the 72 ku protein expressed in ***.

关键词： wheat yellow mosaic virus 72 ku protein prokaryotic cell expression antiserum .

Study of δ-hydrides in Ti-2Al-2.5Zr and Ti-4Al-2V alloys

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Rare Metals 2006年第1期25卷 16-20页

作者： yu Huang GU jialin LIU Qing LIU Yanzhang Department of Materials Science and Engineering Laboratory of Advance Materials Materials Research Center Tsinghua University Beijing100084 China Nuclear Power Institute of China Chengdu 610005 China

The α alloy Ti-2Al-2.5Zr and near α alloy Ti-4Al-2V were hydrogenated to various levels. The morphology, orientation relation （OR）, and habit plane of the hydrides were studied by means of transmission electron microscopy （TEM）. It was found that in the two alloys most of the precipitates are δ-hydrides which have fcc structure with the lattice parameter a = 0.44 nm. Two basic orientation relationships and habit planes of the precipitates are determined. Twin structure was observed in both alloys.

关键词： titanium alloy hydride orientation relation habit plane twin structure

The synthesis and application of zeolitic material from fly ash by one-pot method at low temperature

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Green Energy & Environment 2016年第2期1卷 166-171页

作者： jialin yu Yang Yang Wenting Chen Dong Xu Hua Guo Kevin Li Hanqiang Liu Key Laboratory of Functional Material in Power Generation System Guodian New Energy Technology Research Institute Group for Green Chemistry and Technology Institute of Process EngineeringChinese Academy of Sciences School of Mechanical and Chemical Engineering The University of Western Australia

A new route to prepare zeolitic material was introduced in this work. Compared with traditional methods, the new route showed lower energy consume. The effect of pre-treatment conditions on structure and crystalline phase was investigated, revealing that the mullite crystalline phase in fly ash could be converted to amorphous phase by alkali at low temperature. The removal performance of heavy metal ions on designed material was also investigated, and we found that the intermediate product showed higher adsorption capacity on Ni^(2+) than zeolite A.

关键词： Fly ash Zeolitic material Removal of heavy metal Intermediate

Effects on the local symptoms of subgenomic RNAs expressions and their translational products of Tobacco necrosis virus A Chinese isolate

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Chinese Science Bulletin 2008年第11期53卷 1682-1690页

作者： LI Jiang LI Min LI Cui GAO Yang LI DaWei HAN ChengGui yu jialin State Key Laboratory of Agro-Biotechnology China Agricultural University Beijing 100094 China

Based on a full-length infectious cDNA clone, gene modifications of Tobacco necrosis virus A Chinese isolate (TNV-AC) were made by site-directed mutagenesis or nucleotide deletions for in vitro transcrip-tion of mutant viral RNAs. Mechanical inoculations of Chenopodium amaranticolor with in vitro tran-scripts, containing a single nucleic acid substitution at the presumed transcriptional start sites for the two subgenomic (sg) RNAs, showed that the sgRNA1 and sgRNA2 of TNV-AC were initiated at G2184 or G2460, respectively. Mutagenesis of the translational initiation-codons for the open-reading frame (ORF) P8 or P6 encoded by sgRNA1 indicated that each of the two genes was essential for formation of local lesions on C. amaranticolor leaves, perhaps by blocking virus cell-to-cell movement, but were not necessary for viral RNA replication in the protoplast of tobacco cell BY-2. Results of prokaryotic expression showed that the ORF coding for coat protein on TNV-AC sgRNA2 was initiatively translated by the first AUG codon at nucleotides 2612―2614. Site-directed mutation of translational start codons, and deletion of the entire coding region, showed that the intact TNV-AC coat protein was dispensable for establishment of TNV-AC infection in C. amaranticolor, otherwise the numbers of local lesions and the viral RNA accumulation level were reduced, or the time to symptom appearance significantly delayed. These results suggested that the nucleotide sequence around the translational start codon coding for TNV-AC coat protein gene may play an important role in the local symptoms. Aspects of the involve-ment of the coat protein in the TNV-AC life cycle were discussed.

关键词：烟草坏死病毒A RNAs 染色体基因表达

Functional analysis of beet necrotic yellow vein virus (BNYVV) RNA4 in fungal transmission

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Chinese Science Bulletin 2002年第15期47卷 1281-1284页

作者： HAN Chenggui LI Dawei WANG Dongyong YANG Lili yu jialin CAI Zhunan LIU Yi State Key Laboratory for Agrobiotechnology and Department of Plant Pathology China Agricultural UniversityBeijing 100094China

Full-length RNA4 of beet necrotic yellow vein virus (BNYVV) and its mutants, including a frame-shift and deletions within the coding region, were inserted into a transcription plasmid. Transcripts derived from these plasmids and RNAs extracted from a BNYVV isolate containing only RNAs 1, 2 and 3 were coinoculated to Tetragona expansa. Then these recombinant isolates were inoculated to sugar beet plants grown in a sand culture system containing Po-lymyxa betae that had been freed of virus particles to test efficiency of BNYVV transmission. The result showed that the efficiency of BNYVV transmission among the plants by Polymyxa betae was highly decreased by different deletions in the coding region of RNA4, but not affected by the frame-shift mutant with four nucleotides insertion. The observations suggest that at least some of the RNA4 sequence encompassing the deleted region of 580 nucleotide acids (525 -1105 nt) are necessary for high efficient transmission of the virus by the fungus.

关键词： BNYVV mutation of RNA4 functional analysis trans-mission by Polymyxa betae.

Effect of the RNA5 component on pathogenicity of beet necrotic yellow vein virus

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Chinese Science Bulletin 2003年第8期48卷 796-799页

作者： LIU Tao, HAN Chenggui, LI Dawei, CAI Zhunan, yu jialin & LIU Yi State Key Laboratory for Agrobiotechnology and College of Biological Science, China Agricultural University, Beijing 100094, China Correspondence should be addressed to yu jialin (e-mail: bnyvv@public. ***) State Key Laboratory for Agrobiotechnology and College of Biological Science China Agricultural University

The transcripts of beet necrotic yellow virus RNA5 were prepared from the cloned cDNA in a bacterio-phage T7 in vitro run-off transcription system. The RNA5 transcripts and RNAs extracted from two BNYVV mutants, Hu0 containing RNA1, 2 and Hu3 containing RNA1, 2, 3 respectively, were co-inoculated to Tetragonia expansa and sugar beet seedlings. The results indicated that the virus infections and accumulation in the hosts could be improved by presence of RNA5. Cooperating with RNA3, the RNA5 seems to cause more severe symptom and yield losses in sugar beet. These results suggest that, in addition of RNA3, BNYVV RNA5 is another important factor associated with the virus pathogenicity.

关键词： RNA 致病性甜菜 BNYVV 核糖核酸

Consideration on the Organization of Peasant Market Entry

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Journal of Northeast Agricultural University(English Edition) 2006年第2期13卷 187-191页

作者： FENG Hong yu jialin Liberal and Social Science College Northeast Agricultural University Harbin Heilongjiang 150030 PRC

In the process of agricultural commercialization, it is crucial to develop specialized co-operative organizations as well as to apply initiatives to protect agriculture by the government. Government should organize peasants to enter, adapt and influence the market Peasants should participate and affect policies, monitor the implementation of agricultural protection policy, and form self-protecting mechanism through co-operative organizations.

关键词： agriculture peasants economic co-operation organization