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Numerical analysis of thermal-hydraulic behavior of supercritical water in vertical upward/downward flow channels

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Nuclear Science and Techniques 2008年第3期19卷 178-186页

作者： GU hanyang yu Yiqi CHENG Xu LIU Xiaojing School of Nuclear Science and Engineering Shanghai Jiaotong University Shanghai 200240 China

Investigations on the thermal-hydraulic behavior in the SCWR fuel assembly have obtained a significant attention in the international SCWR community. However, there is still a lack of understanding of the heat transfer behavior of supercritical fluids. In this paper, the numerical analysis is carried out to study the thermal-hydraulic behaviour in vertical sub-channels cooled by supercritical water. Remarkable differences in characteristics of secondary flow are found, especially in square lattice, between the upward flow and downward flow. The turbulence mixing across sub-channel gap for downward flow is much stronger than that for upward flow in wide lattice when the bulk temperature is lower than pseudo-critical point temperature. For downward flow, heat transfer deterioration phenomenon is suppressed with respect to the case of upward flow at the same conditions.

关键词：传热理论热转换数字分析水力行为

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Functional nucleic acids with synthetic sugar or nucleobase moieties

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Advanced Agrochem

Advanced Agrochem 2023年第3期2卷 236-245页

作者： Ze Zhang Siqi Chen Zhe Li hanyang yu State Key Laboratory of Coordination Chemistry Department of Biomedical EngineeringCollege of Engineering and Applied SciencesChemistry and Biomedicine Innovation Center(ChemBIC)Jiangsu Key Laboratory of Artificial Functional MaterialsNanjing UniversityNanjingJiangsu210023China State Key Laboratory of Analytical Chemistry for Life Science Department of Biomedical EngineeringCollege of Engineering and Applied SciencesNanjing UniversityNanjing210023China

Functional nucleic acids including aptamers and DNAzymes are a class of valuable molecular tool for ***,DNA and RNA aptamers and catalysts suffer from low biological stability and limited chemical ***-nucleic acids(XNAs)refer to nucleic acid analogues containing sugar moieties that are structurally distinct from DNA and RNA and possess advantageous *** this article,we first focus on two types of XNAs,threose nucleic acid(TNA)and 2’-fluoroarabinose nucleic acid(FANA),and summarize recent in vitro selections of TNA and FANA aptamers and *** then review three classes of unnatural base pairs(UBPs)and highlight examples of UBP-containing DNA aptamers and ***,we briefly describe an XNA-modified DNAzyme 10–23(X10-23)and its application in RNA knockdown and virus *** XNAs provide important chemical biology tools for biomedical research and future interdisciplinary collaboration will boost XNA basic research and clinical translation.

关键词： properties. sugar base

The induction of PANoptosis in KRAS-mutant pancreatic ductal adenocarcinoma cells by a multispecific platinum complex

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Science China Chemistry 2022年第10期65卷 1978-1984页

作者： Xin yuan Shuren Zhang Xuanmeng Zhong Hao yuan Dongfan Song Xiaoyu Wang hanyang yu Zijian Guo State Key Laboratory of Coordination Chemistry School of Chemistry and Chemical EngineeringChemistry and Biomedicine Innovation Center(ChemBIC)Nanjing UniversityNanjing 210023China Department of Biomedical Engineering College of Engineering and Applied SciencesChemistry and Biomedicine Innovation Center(ChemBIC)Nanjing UniversityNanjing 210023China

Oncogenic KRAS reprograms pancreatic ductal adenocarcinoma(PDAC) cells to a state that is awfully resistant to *** alternative coping strategy is to trigger a nonapoptotic cell ***,a multi specific platinum complex SEP was constructed by conjugating a quinone derivative seratrodast to a prodrug of ***,SEP-treated KRAS-mutant PDAC cells showed the characteristics of pyroptosis,apoptosis and necroptosis,similar to PANoptosis(a newfound inflammatory cell death).Mechanistically,SEP could enter cancer cells effectively,then damage nuclear DNA,boost mitochondrial superoxide anion radicals and affect various signaling pathways related to redox homeostasis and tumor *** our best knowledge,SEP is the first metal complex,even small molecule,to elicit PANoptosis(pyroptosis,apoptosis and necroptosis) in cancer cells,providing a new strategy to overcome apoptotic resistance of KRAS-mutant PDAC.

关键词： metallodrug pancreatic ductal adenocarcinoma PANoptosis cell death platinum prodrug

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Aptamer-based Western blot for selective protein recognition

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Aptamer-based Western blot for selective protein recognition

第十一届全国化学生物学学术会议

作者： Yao Wang Zhe Li hanyang yu Department of Biomedical Engineering College of Engineering and Applied SciencesNanjing University

Selective recognition of proteins is critical in molecular biology techniques such as Western blotting and ELISA. Successful detection of target proteins in Western blotting relies on antibody-antigen interaction, which often requires long incubation time and brings high production cost. Aptamers represent a simple and affordable alternative affinity reagent for protein recognition, and utilizing aptamers in place of antibodies in Western blotting would be potentially more time-and cost-effective.[1] In this work, in vitro selections were performed in parallel to isolate fluorescent DNA aptamers that could selectively label GST-, MBP-and His-tagged proteins immobilized on nitrocellulose membrane. The generated aptamers G1, M1 and H1 specifically bound their target proteins with nanomolar affinity. Such aptamer-based Western blotting gave a cleaner background and could be more specific than conventional antibody-based immunoblotting. Lastly, the identified aptamer sequences were demonstrated to be equally effective in recognition of fusion proteins, thus greatly expanding the scope of potential applications of such aptamers. This work highlighted that the aptamers specifically selected to recognize proteins immobilized on a solid surface represent a type of simpler and more affordable alternative affinity reagent for Western blotting analysis.[2]

关键词： SELEX DNA aptamer Western blot fusion protein recognition

DETERMINATION OF 210Pb IN MARINE SEDIMENTS AND ITS APPLICATION TO GEOCHRONOLOGY

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Acta Oceanologica Sinica 1985年第3期4卷 402-410页

作者：邹汉阳余兴光 Third Institute of Oceanography National Bureau of Oceanography Xiamen

A method of electrodeposit-PbSO4 precipitation-β counting has been developed for the determination of 210Pb in marine sediments. The chemical yield of lead is 85-90%, coincident with radiochemical yield. The decontamination factors for 90Sr, 106Ru, 137Cs, 60Co, 65Zn, 55+59Fe and 54Mn are greater than 103. The precision is 2.9%. The typical 210Pb profiles of five cores were successfully obtained in the Changjiang Estuary and adjacent continental shelf. The sedimentation rates of the cores from five stations of G8132, G8133.G8134, G8135 and G8139 are 0.72, 0.86, 0.37, 0.33 and 0.42 cm/yr respectively.

关键词： Pb IN MARINE SEDIMENTS AND ITS APPLICATION TO GEOCHRONOLOGY DETERMINATION OF

A TNA enzyme capable of discriminating single point mutation

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A TNA enzyme capable of discriminating single point mutation

第十一届全国化学生物学学术会议

作者： yueyao Wang Yao Wang Dongfan Song hanyang yu College of Engineering and Applied Sciences Nanjing University School of Chemistry and Chemical Engineering Nanjing University

Nucleic acid enzymes are single-stranded nucleic acid molecules that catalyze specific chemical *** RNAs found in nature such as group I intron and hammerhead ribozyme catalyze important biochemical reactions and are essential for cell ***,in vitro selections in the laboratory have produced artificial ribozymes and deoxyribozymes with various catalytic activities. Among them,RNA-cleaving nucleic acid enzymes have been developed as novel molecular tools for biosensing and gene silencing ***,the intrinsic low biological stability of DNA and RNA ultimately limit their functions in vivo. Here we describe the evolution of an RNA-cleaving enzyme made of threose nucleic acid(TNA),which is resistant to nuclease degradation and considered an RNA progenitor during early evolution of life on *** show that this TNA enzyme can specifically cleave the model mRNA sequence of EGFR T790 M mutant gene while leaving wild-type substrate *** results demonstrate the capability of TNA to fold into a three-dimensional structure with catalytic property and to perform biologically useful functions.

关键词： Unnatural nucleic acid RNA endonuclease single point discrimination

催化RNA切割反应的苏糖核酸酶的表征及在切割microRNA中的应用

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高等学校化学学报 2023年第3期44卷 182-188页

作者：孙昕高明媚张泽王海燕于涵洋南京大学配位化学国家重点实验室现代工程与应用科学学院化学和生物医药创新研究院江苏省人工功能材料重点实验室南京210023

对一条催化RNA切割反应的苏糖核酸(TNA)酶6-29进行了系统的生物化学表征,探究了TNA酶6-29对RNA底物的序列要求,测定了TNA酶6-29催化反应的酶学常数,并进一步开发了TNA酶6-29在切割microRNA中的应用.研究结果表明,TNA酶6-29能够耐受底物... 详细信息

对一条催化RNA切割反应的苏糖核酸(TNA)酶6-29进行了系统的生物化学表征,探究了TNA酶6-29对RNA底物的序列要求,测定了TNA酶6-29催化反应的酶学常数,并进一步开发了TNA酶6-29在切割microRNA中的应用.研究结果表明,TNA酶6-29能够耐受底物结合臂和切割位点的多种变化,具有良好的底物序列通用性;TNA酶6-29能够在镁离子和锰离子等多种金属离子的存在下发挥催化活性,并且能够催化多个底物分子发生切割反应,具有酶的典型特征.基于TNA酶6-29良好的普适性,进一步展示了TNA酶6-29可以切割不同序列microRNA分子.本文结果表明,具有催化活性的TNA是一种有潜力的分子工具,有望进一步应用于疾病诊疗领域.

关键词：核酸化学非天然核酸苏糖核酸核酶功能核酸

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Direct sequencing of 2’-deoxy-2’-fluoroarabinonucleic acid （FANA） using nanoporeinduced phase-shift sequencing（NIPSS）

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Direct sequencing of 2’-deoxy-2’-fluoroarabinonucleic acid...

第十一届全国化学生物学学术会议

作者： Shuanghong Yan Xintong Li Panke Zhang yuqin Wang Hong-yuan Chen Shuo Huang hanyang yu School of Chemistry and Chemical Engineering Nanjing University Department of Biomedical Engineering College of Engineering and Applied SciencesNanjing University

2’-deoxy-2’-fluoroarabinonucleic acid（FANA）,which is one type of xeno-nucleic acid（XNA）,has been intensively studied in molecular medicine and synthetic biology because of its superior gene-silencing and catalytic *** urgently required,FANA cannot be directly sequenced by any existing *** sequencing,which identifies a single molecule analyte directly from its physical and chemical properties,shows promise for direct XNA *** a proof of concept,different FANA homopolymers show well-distinguished pore blockage signals in a Mycobacterium smegmatis porin A（MspA） *** ligating FANA with a DNA drive-strand,direct FANA sequencing has been demonstrated using phi29 DNA polymerase by Nanopore-Induced Phase Shift Sequencing（NIPSS）.When bound with an FANA template,the phi29 DNA polymerase shows unexpected reverse transcriptase activity when monitored in a single molecule *** further investigations into the ensemble,phi29 DNA polymerase is shown to be a previously unknown reverse transcriptase for FANA that operates at room temperature,and is potentially ideal for nanopore *** results represent the first direct sequencing of a sugar-modified XNA and suggest that phi29 DNA polymerase could act as a promising enzyme for sustained sequencing of a wide variety of XNAs.

关键词： Unnatural nucleic acid Nanopore sequencing

Comparative proteomic analysis of cisplatin-and oxaliplatin-induced phosphorylation in proteins bound to Pt-DNA adducts

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Comparative proteomic analysis of cisplatin-and oxaliplatin-...

第十一届全国化学生物学学术会议

作者： Xin yuan Wanjun Zhang Yafeng He Jian yuan Dongfan Song Weijie Qin hanyang yu Zijian Guo State Key Laboratory of Coordination Chemistry Coordination Chemistry InstituteSchool of Chemistry and Chemical EngineeringNanjing University National Center for Protein Sciences Beijing State Key Laboratory of ProteomicsBeijing Proteome Research CenterBeijing Institute of Lifeomics Department of Biomedical Engineering College of Engineering and Applied SciencesNanjing University

Cisplatin and oxaliplatin are widely used chemotherapeutic agents with different anti-tumor spectra.[1] Cisplatin is believed to covalently bind DNA nucleobases, and such Pt-DNA adducts cause replication and transcription blockage and ultimately leads to apoptosis. Oxaliplatin has been demonstrated to trigger not only DNA damage response but also ribosome biogenesis stress.[2] However, it is not entirely clear how cisplatin and oxaliplatin differs in their mechanisms of inducing cancer cell death. In order to understand the differences in protein phosphorylation patterns caused by cisplatin and oxaliplatin, a label-free proteomic strategy was employed to investigate such damage response processes using Pt-DNA probes. Proteins involved in recognition of specific Pt-DNA lesions were pulled down. Following phosphorylation enrichment and LC-MS analysis, phosphoproteome comparison of colon cancer cells treated with different platinum drugs yielded differentially phosphorylated protein datasets with respect to untreated cells. These identification results clearly show different protein phosphorylation patterns and reflect distinctive mechanisms of actions between cisplatin and oxaliplatin. Such protein phosphorylation patterns may provide novel therapeutic targets and diagnostic biomarkers for the future development of platinum-based drugs.

关键词： cisplatin oxaliplatin Pt-DNA probe phosphoproteome DNA damage response