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Inhibitory effect of synthetic small interfering RNAs on glial fibrillary acidic expression in astrocytes

Neural Regeneration Research 2008年第9期3卷 950-953页

作者： Mingzhu Zhang Qing Zhao Xin Tang guangrong yu Department of Orthopedics First Affiliated Hospital of Dalian Medical University Dalian 116011 Liaoning Province China Depratment of Orthopedics Tongji Hospital of Tongji University Shanghai 200065 China

BACKGROUND： Glial fibritlary acidic protein （GFAP） expression highly correlates with spinal glial scar formation, and is regarded as an important target for scar therapy. Efficient inhibition of expression could benefit recovery from spinal cord injury. OBJECTIVE： To investigate the inhibitory effects of synthetic small interfering RNAs （siRNAs） on astrocytic GFAP expression in rats. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment at the cellular and molecular level was performed at the First Hospital of Dalian Medical University between June 2005 and February 2006. MATERIALS： A total of 100 seven-day-old, Sprague Dawley rats were selected. GAPDH siRNA was purchased from Ambion, USA, And TransMessengerTM Transfection Reagent from DAKO, Carpinteria, CA. METHODS： Rat astrocytes were isolated and cultured. Three pairs of 21-nucleotide （nt） siRNAs specific to rats GFAP mRNA, 401,404 and 854, were synthesized and transfected in primary astrocytes at 1, 2, 3, and 4 g/L using TransMessengerTM Transfection Reagent. Non-transfected astrocytes served as the blank group. Cells transfected with siRNA were regarded as the negative control group, with GAPDH siRNA as the positive control group, and 401 siRNA, 404 siRNA, and 854 siRNA as experimental groups. MAIN OUTCOME MEASURES： GFAP mRNA and protein expression were assessed by RT-PCR and Western blot, respectively, at 24, 48, and 72 hours of culture. RESULTS： GFAP mRNA expression in the positive control group was significantly less than the negative control group （P 〈 0.01）. GFAP mRNA expression in astrocytes from three pairs of siRNA was significantly less than the blank group after 48 hours （P 〈 0.01 ）, while no differences were detected between the negative control and blank groups （P 〉 0.05）. GFAP protein expression was remarkably less in siRNA-transfected astrocytes compared to the blank control （P 〈 0.01 ）. CONCLUSION： Transfected siRNAs could significantly inhibit GFAP gene expression in astrocytes a

关键词： glial fibrillary acidic protein RNA interference spinal cord injury

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Deformation and stress distribution of the human foot after plantar ligaments release:A cadaveric study and finite element analysis

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Science China(Life Sciences) 2011年第3期54卷 267-271页

作者： LIANG Jun YANG yunFeng yu guangrong NIU WenXin WANG yuBin Sports Medicine Department of Shanghai East Hospital Tongji University Shanghai 200120 China Orthopaedic Department of Tongji Hospital Tongji University Shanghai 200065 China School of Biological Science and Medical Engineering Beihang University Beijing 100191 China

The majority of foot deformities are related to arch collapse or instability,especially the longitudinal *** the relationship between the plantar fascia and arch height has been previously investigated,the stress distribution remains *** aim of this study was to explore the role of the plantar ligaments in foot arch *** constructed a geometrical detailed three-dimensional (3-D) finite element (FE) model of the human foot and ankle from computer tomography *** model comprised the majority of joints in the foot as well as bone segments,major ligaments,and plantar soft *** of the plantar fascia and other ligaments was simulated to evaluate the corresponding biomechanical effects on load distribution of the bony and ligamentous *** intrinsic ligaments of the foot arch were sectioned to simulate different pathologic situations of injury to the plantar ligaments,and to explore bone segment displacement and stress *** validity of the 3-D FE model was verified by comparing results with experimentally measured data via the displacement and von Mise stress of each bone *** fascia release decreased arch height,but did not cause total collapse of the foot *** longitudinal foot arch was lost when all the four major plantar ligaments were sectioned *** fascia release was compromised by increased strain applied to the plantar ligaments and intensified stress in the midfoot and metatarsal *** redistribution among the centralized metatarsal bones and focal stress relief at the calcaneal insertion were *** 3-D FE model indicated that plantar fascia release may provide relief of focal stress and associated heel ***,these operative procedures may pose a risk to arch stability and clinically may produce dorsolateral midfoot *** initial strategy for treating plantar fasciitis should be non-operative.

关键词： biomechanics finite element analysis foot arch ligament stress

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Effect of fixing distal radius fracture with volar locking palmar plates while preserving pronator quadratus

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Chinese Medical Journal 2014年第16期127卷 2929-2933页

作者： Fan Jian Chen Kai Zhu Hui Jiang Bo yuan Feng Zhu Xiaozhong Mei Jiong yu guangrong Department of Orthopedics Tongji Hospital Tongji UniversityShanghai 200065 China

Background L-shaped incision of pronator quadratus （PQ） muscle along its radial and distal borders was always taken for distal radius fractures reduction and internal *** of the PQ muscle was always recommended at the end of operation for some instructive *** repair of PQ is not satisfied because of poor quality of muscle and fascial tissues which may cause pain or impede forearm pronation and supination for post-operative scarring around *** the locking palmar plate to pass under the pronator quadratus muscle and the locking screws are inserted through miniincisions in pronator quadratus in some patients with distal radius fractures is a reasonable technique which can preserve the pronator *** purpose of this study was to evaluate and compare the clinical effects after volar plating of the distal radius fractures while preserving the pronator quadratus and pronator quadratus *** Between September 2010 and April 2012,65 patients （42 males and 23 females; aged 20-68 years and a mean age of 42.5 years） with distal radius fracture underwent open reduction and internal fixation using the volar locking palmar plates （Depuy or Smith companies）.The patients were classified as 23A-2 through 23C-3 according to the Orthopaedic Trauma Association （OTA） *** surgeries were completed by the same trained *** volar locking palmar plates of distal radius performed with preserving pronator quadratus group involved 30 patients including 19 males and 11 females and performed with pronator quadratus repair group involved 35 patients including 23 males and 12 *** compared the two groups for wrist pain,forearm range of motion,grip strength,pedoperative complications and wrist functional recovery *** The minimum follow-up for the whole cohort was one *** differences between the two groups were significant with regard to wrist pain,forearm range of motion,grip strength and wrist function at 1,2,and

关键词： distal radius fracture open reduction palmar plate pronator quadratus

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改良Kidner术治疗Ⅱ型痛性副舟骨的临床疗效

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中国现代手术学杂志 2024年第3期28卷 208-211页

作者：王振明晓锋王社言刘青冯彦江赵栋杨波俞光荣河南省洛阳正骨医院(河南省骨科医院)足踝外三科河南郑州450000 同济大学附属同济医院足踝外科上海200065

目的探讨改良Kidner术治疗Ⅱ型痛性副舟骨的临床疗效。方法回顾性分析2018年5月至2020年8月诊治的36例Ⅱ型痛性副舟骨患者的临床资料,均采用改良Kidner术(胫后肌腱转位于足舟骨跖内侧后,用铆钉固定胫后肌腱于足舟骨)。结果本组均顺利完... 详细信息

目的探讨改良Kidner术治疗Ⅱ型痛性副舟骨的临床疗效。方法回顾性分析2018年5月至2020年8月诊治的36例Ⅱ型痛性副舟骨患者的临床资料,均采用改良Kidner术(胫后肌腱转位于足舟骨跖内侧后,用铆钉固定胫后肌腱于足舟骨)。结果本组均顺利完成手术,手术时间平均45(35~60)min,术中出血量平均8(5~10)mL。术后切口均一期愈合。36例均获随访,随访时间平均15(12~24)个月。随访期间未出现铆钉或肌腱松动情况。末次随访踝与后足功能AOFAS评分平均93(85~99)分。结论改良Kidner术治疗Ⅱ型痛性副舟骨临床效果确切,创伤小,操作简单。

关键词：痛性足副舟骨改良Kidner术

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关于发展繁荣四川地方高校社会科学研究的理性思考——以四川师范大学为重点

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四川师范大学学报（社会科学版） 2007年第6期34卷 74-82页

作者：庾光蓉四川师范大学科研处成都610068

四川地方高校社科研究力量分散,缺乏承担重大项目的能力,且水平不高,能力不强。如何加强社科研究力量和方向的整合,以科学发展观为指导,认真探索社会科学研究管理规律,以市场需求与社会需求为切入点,广泛开展校地合作,尽快形成地域性的... 详细信息

四川地方高校社科研究力量分散,缺乏承担重大项目的能力,且水平不高,能力不强。如何加强社科研究力量和方向的整合,以科学发展观为指导,认真探索社会科学研究管理规律,以市场需求与社会需求为切入点,广泛开展校地合作,尽快形成地域性的研究中心,为繁荣哲学社会科学创造良好环境,是四川地方高校社会科学研究发展的基础。

关键词：四川地方高校社会科学研究现状思考

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跟骨骨折的治疗策略

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上海医学 2005年第7期28卷 541-543页

作者：俞光荣同济大学医学院足踝外科研究所上海200065 同济大学附属同济医院骨科

跟骨骨折是最常见的跗骨骨折，其治疗方法很多，各有相应的适应证和优缺点，应根据损伤程度、骨折类型、全身和局部情况以及技术条件，合理选择治疗方法，以提高临床疗效。[第一段]

关键词：跟骨骨折治疗策略治疗方法跗骨骨折损伤程度骨折类型技术条件临床疗效优缺点

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努力提高关节周围骨折的临床疗效

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上海医学 2007年第8期30卷 567-569页

作者：俞光荣同济大学附属同济医院骨科上海200065

关节周围骨折尤其是复杂骨折，多需手术治疗，但疗效难尽如人意，是创伤骨科临床的一大难点。随着交通和建筑工地伤的日益增多，及人口的老龄化，关节内及干骺端骨折也呈上升趋势。如肱骨近端骨折约占全身骨折的2.5％，国外文献报道占4... 详细信息

关节周围骨折尤其是复杂骨折，多需手术治疗，但疗效难尽如人意，是创伤骨科临床的一大难点。随着交通和建筑工地伤的日益增多，及人口的老龄化，关节内及干骺端骨折也呈上升趋势。如肱骨近端骨折约占全身骨折的2.5％，国外文献报道占4％～5%；Pilon骨折约占下肢骨折的1％。关节内骨折时，关节面骨折块问常存在间隙或台阶，合并干骺端粉碎。其畸形愈合可表现为关节面不平整，肢体轴线及关节运动轨迹异常，关节僵硬、疼痛、不稳定，关节软骨破坏。

关键词：关节周围骨折临床疗效关节面不平整 Pilon骨折干骺端骨折关节内骨折肱骨近端骨折关节软骨破坏

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髓内钉胫距跟融合术并发症的研究进展

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中国修复重建外科杂志 2015年第9期29卷 1173-1176页

作者：冯骏俞光荣同济大学附属同济医院骨科上海200333

目的综述髓内钉胫距跟融合术相关并发症的发生原因、处理及预防方法,旨在提高对胫距跟融合术并发症的认识。方法查阅近年髓内钉胫距跟融合术的相关文献,对其并发症相关内容进行综述分析。结果髓内钉胫距跟融合术的并发症可分为术中和术... 详细信息

目的综述髓内钉胫距跟融合术相关并发症的发生原因、处理及预防方法,旨在提高对胫距跟融合术并发症的认识。方法查阅近年髓内钉胫距跟融合术的相关文献,对其并发症相关内容进行综述分析。结果髓内钉胫距跟融合术的并发症可分为术中和术后两大类,产生并发症的原因多种多样,针对不同并发症处理和预防方法也不同。结论髓内钉胫距跟融合技术的进步提高了关节融合成功率,但手术并发症发生率仍较高,需要严格的术前评估、熟练的手术技术来预防。

关键词：髓内钉胫距跟融合术并发症

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