检索结果-南通市图书馆

Neuroprotective and anti-inflammatory effects of eicosane on glutamate and NMDA-induced retinal ganglion cell injury

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2024年第4期17卷 638-645页

作者： Zhen-Kai Wu Huan-yu Li You-Lin Zhu Meng-qin Xiong Jing-Xiang Zhong Department of Ophthalmology the First Affiliated Hospital of Jinan UniversityGuangzhou 510632Guangdong ProvinceChina Changde Hospital Xiangya School of MedicineCentral South University(the First People’s Hospital of Changde City)Changde 415000Hunan ProvinceChina

AIM:To investigate the protective effects,antioxidant potential,and anti-inflammatory mechanisms of eicosane on glutamate-induced cell damage and on N-methyl-Daspartate(NMDA)-induced retinal ganglion cell(RGC)injury in a mouse model of ***:The protective effects of eicosane on the rat R28 retinal precursor cell line were assessed using cell counting kit-8 assays and Hoechst-propidium iodide *** reactive oxygen species(ROS)production was measured using the fluorescent probe 2'-7'-dichlorofluorescin diacetate and flow *** protective role of eicosane on NMDA-induced RGC injury in a mouse glaucoma model was determined by immunostaining of frozen sections of *** effects of eicosane on the metabolome of the retina in mice with NMDA-induced RGC damage were evaluated by liquid chromatographymass spectroscopy(LC-MS)and untargeted metabolomics ***:Eicosane treatment significantly attenuated glutamate-induced damage to R28 cells in *** also protected RGCs against NMDA-induced injury in a mouse glaucoma *** metabolomics analyses showed that eicosane increased multiple metabolites,including L-arginine and L-carnitine,in the ***:Eicosane has protective effects,antioxidant potential,and anti-inflammatory properties in an in vitro model of glutamate-induced cell damage and in an in vivo model of NMDA-induced RGC injury in mouse glaucoma through modulation of L-arginine and/or L-carnitine metabolism.

关键词： glaucoma metabolites eicosane

Detachable string magnetically controlled capsule endoscopy for the noninvasive diagnosis of esophageal diseases:A prospective,blind clinical study

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2024年第9期30卷 1121-1131页

作者： Yan-Ling Yang Huang-Wen qin Zhao-yu Chen Hui-Ning Fan Yi yu Wei Da Jin-Shui Zhu Jing Zhang Department of Gastroenterology Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200233China

BACKGROUND Traditional esophagogastroduodenoscopy(EGD),an invasive examination method,can cause discomfort and pain in *** contrast,magnetically controlled capsule endoscopy(MCE),a noninvasive method,is being applied for the detection of stomach and small intestinal diseases,but its application in treating esophageal diseases is not *** To evaluate the safety and efficacy of detachable string MCE(ds-MCE)for the diagnosis of esophageal *** Fifty patients who had been diagnosed with esophageal diseases were pros-pectively recruited for this clinical study and underwent ds-MCE and conven-tional *** primary endpoints included the sensitivity,specificity,positive predictive value,negative predictive value,and diagnostic accuracy of ds-MCE for patients with esophageal *** secondary endpoints consisted of visualizing the esophageal and dentate lines,as well as the subjects'tolerance of the *** Using EGD as the gold standard,the sensitivity,specificity,positive predictive value,negative predictive value,and diagnostic accuracy of ds-MCE for esophageal disease detection were 85.71%,86.21%,81.82%,89.29%,and 86%,***-MCE was more comfortable and convenient than EGD was,with 80%of patients feeling that ds-MCE examination was very comfortable or comfortable and 50%of patients believing that detachable string v examination was very *** This study revealed that ds-MCE has the same diagnostic effects as traditional EGD for esophageal diseases and is more comfortable and convenient than EGD,providing a novel noninvasive method for treating esophageal diseases.

关键词： Clinical trial Detachable string magnetically controlled capsule endoscopy Esophagogastroduodenoscopy Noninvasive diagnosis Esophageal diseases

To explore the pathogenesis of anterior resection syndrome by magnetic resonance imaging rectal defecography

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Surgery 2024年第2期16卷 529-538页

作者： Ling-Hou Meng Xian-Wei Mo Bing-yu Yang Hai-Quan qin qing-Zhou Song Xin-Xin he Qiang Li Zheng Wang Chang-Lin Mo Guo-Hai Yang Department of Gastrointestinal Surgery Guangxi Clinical Research Center for Colorectal CancerGuangxi Medical University Cancer HospitalNanning 530021Guangxi Zhuang Autonomous RegionChina Department of Colorectal and Anal Surgery Guangxi Medical University Cancer HospitalNanning 530021Guangxi Zhuang Autonomous RegionChina Department of Radiology Guangxi Medical University Cancer HospitalNanning 530021Guangxi Zhuang Autonomous RegionChina First Department of Chemotherapy Guangxi Medical University Cancer HospitalNanning 530021Guangxi Zhuang Autonomous RegionChina

BACKGROUND Over 90%of rectal cancer patients develop low anterior resection syndrome(LARS)after sphincter-preserving *** current globally recognized evaluation method has many drawbacks and its subjectivity is too strong,which hinders the research and treatment of *** To evaluate the anorectal function after colorectal cancer surgery by quantifying the index of magnetic resonance imaging(MRI)defecography,and pathogenesis of *** We evaluated 34 patients using the standard LARS score,and a new LARS evaluation index was established using the dynamic images of MRI defecography to verify the LARS *** In the LARS score model,there were 10(29.41%)mild and 24(70.58%)severe cases of *** comparison of defecation rate between the two groups was 29.36±14.17%versus 46.83±18.62%(P=0.004);and MRI-rectal compliance(MRI-RC)score was 3.63±1.96 versus 7.0±3.21(P=0.001).Severe and mild LARS had significant differences using the two evaluation *** was a significant negative correlation between LARS and MRI-RC score(Phey had a negative correlation with defecation rate(P=0.028).CONCLUSION MRI defecography and standard LARS score can both be used as an evaluation index to study the pathogenesis of LARS.

关键词： Anterior resection syndrome Colorectal cancer Diagnostic evaluation system Magnetic resonance imaging defecography Pathogenesis

A highly sensitive fluorescent probe RN-NA reveals peroxynitrite as a novel biomarker for primary open angle glaucoma

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2024年第2期17卷 219-227页

作者： Bo qin yuan Lei Luo Bao You-Jia Zhang Ke-Yin Liu yu-Hong Chen Department of Ophthalmology&Visual Science Eye&ENT HospitalShanghai Medical CollegeFudan UniversityShanghai 200031China Key Laboratory of Myopia NHFPC(Fudan University)Shanghai Key Laboratory of Visual Impairment and Restoration(Fudan University)Shanghai 200031China State Key Laboratory of Biobased Material and Green Papermaking Key Laboratory of Pulp&Paper Science and Technology of Shandong Province/Ministry of EducationQilu University of TechnologyShandong Academy of SciencesJinan 250353Shandong ProvinceChina

AIM:To directly quantify peroxynitrite(ONOO-)using a highly sensitive fluorescence resonance energy transfer probe RN-NA,investigate the association between ONOOand primary open angle glaucoma(POAG),and clarify whether RN-NA could be used as a potential tool for POAG ***:Plasma and aqueous humor(AH)samples were collected from POAG patients(n=100,age:59.70±6.87y)and age-related cataract(ARC)patients(n=100,age:61.15±4.60y)admitted to our ***,RN-NA was used to detect ONOO-in plasma and AH samples,and the relationship between ONOO-level and POAG was analyzed using binary logistic ***,Pearson correlation analysis was applied to characterize the correlation of the levels of ONOO-with the patients’age,intraocular pressure(IOP),and mean deviation of visual field *** ONOO-scavenger MnTMPyP was employed to treat the 3-morpholinosyndnomine(SIN-1)-induced ocular hypertension in mice(n=7,6-8wk).Finally,the IOP and ONOO-in both eyes were measured 30min after the last drug ***:ONOO-levels of AH and plasma were significantly higher in the POAG group than in the ARC group(Phe POAG group(R=0.69,Phe elevated IOP caused by SIN-1(P<0.05).CONCLUSION:ONOO-levels in AH and plasma,detected by RN-NA,are significantly related to POAG and positively correlated with visual field defects in POAG ***,ONOO-is a potential biomarker of POAG,especially advanced ***,anti-nitration compounds may be novel ocular hypotens

关键词： peroxynitrite fluorescent probe RN-NA primary open angle glaucoma 3-morpholinosyndnomine

Impact of metabolic syndrome components on clinical outcomes in hypertriglyceridemia-induced acute pancreatitis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2024年第35期30卷 3996-4010页

作者： Zhen-Hua Fu Zi-yue Zhao Yao-Bing Liang Dong-yu Cheng Jian-Ming Luo Hai-Xing Jiang Shan-yu qin Department of Gastroenterology The First Affiliated Hospital of Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina

BACKGROUND The incidence of hypertriglyceridemia(HTG)-induced acute pancreatitis(AP)is steadily increasing in China,becoming the second leading cause of *** complications and outcomes associated with HTG-AP are generally more severe than those seen in AP caused by other ***-AP is closely linked to metabolic dysfunction and frequently coexists with metabolic syndrome or its ***,the impact of metabolic syndrome components on HTGAP clinical outcomes remains *** To investigate the impact of metabolic syndrome component burden on clinical outcomes in *** In this retrospective study of 255 patients diagnosed with HTG-AP at the First Affiliated Hospital of Guangxi Medical University,we collected data on patient demographics,clinical scores,complications,and clinical ***,we analyzed the influence of the presence and number of individual metabolic syndrome components,including obesity,hyperglycemia,hypertension,and low high-density lipoprotein cholesterol(HDL-C),on the aforementioned parameters in HTG-AP *** This study found that metabolic syndrome components were associated with an increased risk of various complications in HTG-AP,with low HDL-C being the most significant risk factor for clinical *** risk of complications increased with the number of metabolic syndrome *** for age and sex,patients with highcomponent metabolic syndrome had significantly higher risks of renal failure[odds ratio(OR)=3.02,95%CI:1.12-8.11)],SAP(OR=5.05,95%CI:2.04-12.49),and intensive care unit admission(OR=6.41,95%CI:2.42-16.97)compared to those without metabolic *** The coexistence of multiple metabolic syndrome components can synergistically worsen the clinical course of HTGAP,making it crucial to monitor these components for effective disease management.

关键词： Hypertriglyceridemia-induced acute pancreatitis Metabolic syndrome High density lipoprotein cholesterol Obesity Hyperglycemia Hypertension Clinical outcomes

Gene cloning,protein expression,and enzymatic characterization of a double-stranded RNA degrading enzyme in Apolygus lucorum

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2024年第1期31卷 119-133页

作者： Jie-yu Zhang Jing Zhao Keyan Zhu-Salzman qin-qin Ji Yi-Ping Jiang Liu-Bin Xiao De-Jin Xu Guang-Chun Xu Lin-Quan Ge Yong-An Tan College of Plant Protection Yangzhou UniversityYangzhouJiangsu ProvinceChina Institute of Plant Protection Jiangsu Academy of Agricultural SciencesNanjingJiangsu ProvinceChina Department of Entomology Texas A&M UniversityCollege StationTXUSA Taizhou Customs of the People's Republic of China TaizhouJiangsu ProvinceChina

RNA interference(RNAi)is a powerful tool that post-transcriptionally silences target genes in eukaryotic ***,silencing efficacy varies greatly among different insect ***,we met with little success when attempting to knock down genes in the mirid bug Apolygus lucorum via dsRNA *** disappearance of double-stranded RNA(dsRNA)could be a potential factor that restricts RNAi ***,we found that dsRNA can be degraded in midgut fluids,and a dsRNase of ***(AldsRNase)was identified and *** alignment indicated that its 6 key amino acid residues and the Mg2+-binding site were similar to those of other insects’*** signal peptide and endonuclease non-specific domain shared high sequence identity with the brown-winged green stinkbug Plautia stali *** showed high salivary gland and midgut expression and was continuously expressed through the whole life cycle,with peaks at the 4th instar ecdysis in the whole *** purified AldsRNase protein obtained by heterologously expressed can rapidly degrade *** comparing the substrate specificity of AldsRNase,3 specific substrates(dsRNA,small interfering RNA,and dsDNA)were all degraded,and the most efficient degradation is ***,immunofluorescence revealed that AldsRNase was expressed in the cytoplasm of midgut *** cloning and functional study of AldsRNase,the enzyme activity and substrate specificity of the recombinant protein,as well as the subcellular localization of nuclease,the reason for the disappearance of dsRNA was explained,which was useful in improving RNAi efficiency in *** and related species.

关键词： Apolygus lucorum cloning dsRNase enzymatic activity protein expression

Autoimmune hepatitis-primary biliary cholangitis overlap syndrome complicated by various autoimmune diseases:A case report

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2024年第6期12卷 1174-1181页

作者： yu-Jie qin Ting Gao Xing-Nian Zhou Ming-Liang Cheng Hong Li Clinical Medicine School of Guizhou Medical University Guiyang 550025Guizhou ProvinceChina Department of Pathology The Affiliated Hospital of Guizhou Medical UniversityGuiyang 550025Guizhou ProvinceChina Department of Infectious Diseases Guizhou Medical UniversityGuiyang 550025Guizhou ProvinceChina Department of Infectious Diseases Guizhou Provincial People's HospitalGuiyang 550025Guizhou ProvinceChina

BACKGROUND Autoimmune hepatitis(AIH)and primary biliary cholangitis(PBC)are two common clinical autoimmune liver diseases,and some patients have both diseases;this feature is called AIH-PBC overlap *** thyroid disease(AITD)is the most frequently overlapping extrahepatic autoimmune ***(IgG)4-related disease is an autoimmune disease recognized in recent years,characterized by elevated serum IgG4 levels and infiltration of IgG4-positive plasma cells in *** SUMMARY A 68-year-old female patient was admitted with a history of right upper quadrant pain,anorexia,and jaundice on physical *** examination revealed elevated liver enzymes,multiple positive autoantibodies associated with liver and thyroid disease,and imaging and biopsy suggestive of pancreatitis,hepatitis,and PBC.A diagnosis was made of a rare and complex overlap syndrome of AIH,PBC,AITD,and IgG4-related *** features improved on treatment with ursodeoxycholic acid,methylprednisolone,and *** This case highlights the importance of screening patients with autoimmune diseases for related conditions.

关键词： Overlap syndrome Autoimmune hepatitis Primary biliary cholangitis Primary sclerosing cholangitis Autoimmune thyroid disease Case report

Multi-agent policy transfer via task relationship modeling

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Information Sciences) 2024年第8期67卷 102-114页

作者： Rongjun qin Feng CheN Tonghan WANG Lei yuAN Xiaoran WU Yipeng KANG Zongzhang ZHANG Chongjie ZHANG Yang yu National Key Laboratory for Novel Software Technology Nanjing University Polixir Technologies School of Engineering and Applied Sciences Harvard University Department of Computer Science and Technology Tsinghua University Institute for Interdisciplinary Information Sciences Tsinghua University

Team adaptation to new cooperative tasks is a hallmark of human intelligence, which has yet to be fully realized in learning agents. Previous studies on multi-agent transfer learning have accommodated teams of different sizes but heavily relied on the generalization ability of neural networks for adapting to unseen tasks. We posit that the relationship among tasks provides key information for policy *** utilize this relationship for efficient transfer by attempting to discover and exploit the knowledge among tasks from different teams, proposing to learn an effect-based task representation as a common latent space among tasks, and using it to build an alternatively fixed training scheme. herein, we demonstrate that task representation can capture the relationship among teams and generalize to unseen tasks. Thus, the proposed method helps transfer the learned cooperation knowledge to new tasks after training on a few source ***, the learned transferred policies help solve tasks that are difficult to learn from scratch.

关键词： multi-agent reinforcement learning cooperative transfer learning task relationship modeling multi-agent policy reuse multi-agent multi-task learning

Construction of an alternative NADPH regeneration pathway improves ethanol production in Saccharomyces cerevisiae with xylose metabolic pathway

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Synthetic and Systems Biotechnology 2024年第2期9卷 269-276页

作者： Yali Qiu Wei Liu Meiling Wu Haodong Bao Xinhua Sun qin Dou Hongying Jia Weifeng Liu yu Shen State Key Laboratory of Microbial Technology Institute of Microbial TechnologyShandong UniversityQingdao266237China Advanced Medical Research Institute Shandong UniversityJinan250012China

Full conversion of glucose and xylose from lignocellulosic hydrolysates is required for obtaining a high ethanol ***,glucose and xylose share flux in the pentose phosphate pathway(PPP)and glycolysis pathway(EMP),with glucose having a competitive advantage in the shared metabolic *** this work,we knocked down ZWF1 to preclude glucose from entering the *** reduced the[NADPH]level and disturbed growth on both glucose or xylose,confirming that the oxidative PPP,which begins with Zwf1p and ultimately leads to CO_(2) production,is the primary source of NADPH in both glucose and *** glucose depletion,gluconeogenesis is necessary to generate glucose-6-phosphate,the substrate of *** re-established the NADPH regeneration pathway by replacing the endogenous NAD^(+)-dependent glyceraldehyde-3-phosphate dehydrogenase(GAPDH)gene TDH3 with heterogenous NADP^(+)-GAPDH genes GDH,gapB,and *** the resulting strains,the strain BZP1(zwf1Δ,tdh3::GDP1)exhibited a similar xylose consumption rate before glucose depletion,but a 1.6-fold increased xylose consumption rate following glucose depletion compared to the original strain BSGX001,and the ethanol yield for total consumed sugars of BZP1 was 13.5%higher than *** suggested that using the EMP instead of PPP to generate NADPH reduces the wasteful metabolic cycle and excess CO_(2)^(++) release from oxidative ***,we used a copper-repressing promoter to modulate the expression of ZWF1 and optimize the timing of turning off the ZWF1,therefore,to determine the competitive equilibrium between glucose-xylose *** strategy allowed fast growth in the early stage of fermentation and low waste in the following stages of fermentation.

关键词： Saccharomyces cerevisiae Xylose Ethanol NADPH Glyceraldehyde-3-phosphate dehydrogenase