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Inhibitor of DNA binding 2 accelerates nerve regeneration after sciatic nerve injury in mice

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Neural Regeneration Research 2021年第12期16卷 2542-2548页

作者： Zhong-Hai Huang Ai-Ying Feng Jing Liu Libing Zhou Bing Zhou panpan yu Guangdong-Hong Kong-Macao Institute of Central Nervous System Regeneration Ministry of Education Joint International Research Laboratory of Central Nervous System RegenerationJinan UniversityGuangzhouGuangdong ProvinceChina Interdisciplinary Innovation Institute of Medicine and Engineering Beijing Advanced Innovation Center for Big Data-Based Precision MedicineBeihang UniversityBeijingChina

Inhibitor of DNA binding 2(Id2)can promote axonal regeneration after injury of the central nervous ***,whether Id2 can promote axonal regeneration and functional recovery after peripheral nerve injury is currently *** this study,we established a mouse model of bilateral sciatic nerve crush *** weeks before injury,AAV9-Id2-3×Flag-GFP was injected stereotaxically into the bilateral ventral horn of lumbar spinal *** results showed that Id2 was successfully delivered into spinal cord motor neurons projecting to the sciatic nerve,and the number of regenerated motor axons in the sciatic nerve distal to the crush site was increased at 2 weeks after injury,arriving at the tibial nerve and reinnervating a few endplates in the gastrocnemius *** 1 month after injury,extensive neuromuscular reinnervation *** addition,the amplitude of compound muscle action potentials of the gastrocnemius muscle was markedly recovered,and their latency was *** findings suggest that Id2 can accelerate axonal regeneration,promote neuromuscular reinnervation,and enhance functional improvement following sciatic nerve ***,elevating the level of Id2 in adult neurons may present a promising strategy for peripheral nerve repair following *** study was approved by the Experimental Animal Ethics Committee of Jinan University(approval No.20160302003)on March 2,2016.

关键词： axonal regeneration functional recovery inhibitor of DNA binding 2 motor neuron neuromuscular junctions peripheral nerve reinnervation sciatic nerve injury

Effect of chondroitin sulfate proteoglycans on neuronal cell adhesion, spreading and neurite growth in culture

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Neural Regeneration Research 2018年第2期13卷 289-297页

作者： Jingyu Jin Sharada Tilve Zhonghai Huang Libing Zhou Herbert M.Geller panpan yu Guangdong-Hongkong-Macao Institute of CNS Regeneration Ministry of Education Joint International Research Laboratory of CNS Regeneration Jinan University Laboratory of Developmental Neurobiology Cell Biology and Physiology Center National Heart Lung and Blood Institute National Institutes of Health

As one major component of extracellular matrix （ECM） in the central nervous system, chondroitin sul- fate proteoglycans （CSPGs） have long been known as inhibitors enriched in the glial scar that prevent axon regeneration after injury. Although many studies have shown that CSPGs inhibited neurite out- growth in vitro using different types of neurons, the mechanism by which CSPGs inhibit axonal growth remains poorly understood. Using cerebellar granule neuron （CGN） culture, in this study, we evaluated the effects of different concentrations of both immobilized and soluble CSPGs on neuronal growth, in- cluding cell adhesion, spreading and neurite growth. Neurite length decreased while CSPGs concentration arised, meanwhile, a decrease in cell density accompanied by an increase in cell aggregates formation was observed. Soluble CSPGs also showed an inhibition on neurite outgrowth, but it required a higher concen- tration to induce cell aggregates formation than coated CSPGs. We also found that growth cone size was significantly reduced on CSPGs and neuronal cell spreading was restrained by CSPGs, attributing to an inhibition on lamellipodial extension. The effect of CSPGs on neuron adhesion was further evidenced by interference reflection microscopy （IRM） which directly demonstrated that both CGNs and cerebral cortical neurons were more loosely adherent to a CSPG substrate. These data demonstrate that CSPGs have an effect on cell adhesion and spreading in addition to neurite outgrowth.

关键词： chondroitin sulfate proteoglycans cell adhesion neurite growth interference reflection microscopy neural regeneration

A new fluorescent quinoline derivative toward the acid-responsivity in both solution and solid states

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Chinese Chemical Letters 2020年第11期31卷 2909-2912页

作者： Pu Wang Dan Liu Yongshuai Wang Pan Zhang panpan yu Mincan Wang Yonggang Zhen Huanli Dong Wenping Hu College of Chemistry and Institute of Green Catalysis Zhengzhou UniversityZhengzhou 450052China Beijing National Laboratory for Molecular Sciences Key Laboratory of Organic SolidsInstitute of ChemistryChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China Beijing Key Laboratory for Optical Materials and Photonic Devices Department of ChemistryCapital Normal UniversityBeijing 100048China Collaborative Innovation Center of Chemical Science and Engineering(Tianjin)&Department of Chemistry School of ScienceTianjin UniversityTianjin 300072China

In this article,an acid-responsive luminescent material,1,4-di(quinoline-6-yl)buta-1,3-diyne(DQBD)is designed and *** different pH values,gradual changes of fluorescence colors for DQBD in both solution and solid phases are demonstrated due to the protonation ***,such responsive characteristics can also be reversible,suggesting DQBD as a promising fluorescent material with great potential for reusable-and accurate-p H sensors in the future.

关键词： Quinoline derivative Stimuli-responsive Protonation effect Fluorescence switch pH sensor

Long noncoding RNA(lncRNA)HOTAIR:Pathogenic roles and therapeutic opportunities in gastric cancer

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Genes & Diseases 2022年第5期9卷 1269-1280页

作者： Wencheng Kong Guang Yin Sixin Zheng Xinchun Liu Akao Zhu panpan yu Jian Zhang yuqiang Shan Rongchao Ying Huicheng Jin Department of Gastroenterological Surgery Affiliated Hangzhou First People’s HospitalZhejiang University School of MedicineHangzhouZhejiang 310006PR China

Gastric cancer is one of the first malignant cancers in the world and a large number of people die every year due to this *** genetic and epigenetic risk factors have been identified that play a major role in gastric *** is an effective epigenetic agent known as long noncoding RNA(lncRNA).HOTAIR has been described to have biological functions in biochemical and cellular processes through interactions with many factors,leading to genomic stability,proliferation,survival,invasion,migration,metastasis,and drug *** the present article,we reviewed the prognostic value of the molecular mechanisms underlying the HOTAIR regulation and its function in the development of Gastric Cancer,whereas elucidation of HOTAIR–protein and HOTAIR–DNA interactions can be helpful in the identification of cancer processes,leading to the development of potential therapeutic strategies.

关键词： Expression Gastric cancer HOTAIR regulation Regulatory pathways

Dynamic 3D holographic projection of vectorial images with a multimode fiber

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Photonics Research 2024年第6期12卷 1344-1350页

作者： JINGHAN ZHUANG panpan yu YIFAN LIU YIJING WU ZIQIANG WANG YINMEI LI LEI GONG Department of Optics and Optical Engineering University of Science and Technology of ChinaHefei 230026China Advanced Laser Technology Laboratory of Anhui Province Hefei 230026China

An optical multimode fiber(MMF)is capable of delivering structured light modes or complex images with high ***,we present a holographic approach to enable the MMF as a 3D holographic projector with the capability of complete polarization *** harnessing the strong coupling of the spatial and polarization degrees of freedom of light propagating through MMFs,our approach realizes active control of the output intensity and polarization in 3D space by shaping only the wavefront of the incident *** this manner,we demonstrate MMF-based holographic projection of vectorial images on multiple planes via a phase-only ***,dynamic projection of polarization-multiplexed grayscale images is presented with an averaged Pearson correlation coefficient of up to *** work is expected to benefit fiber-based holographic displays,data transmission,optical imaging,and manipulation.

关键词： holographic projection enable

Comparision of two sequencing methods in the complete mitochondrial genome of Epeorus *** the gene arrangement of Epeorus sp.

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Comparision of two sequencing methods in the complete mitoch...

Effects of SHANK2 gene knockdown on early neural development of induced pluripotent stem cell derived neuron

浙江省动物学会第十二届会员代表大会暨学术讨论会

作者： panpan yu Tingting Wang Xuefang Cheng yue Ma Jiayong Zhang College of Chemistry and Life Science Zhejiang Normal University

Next-generation sequencing(NGS) approach via Long-Range PCR and the classical sequencing via short fragments amplification can successfully obtain the mitochondrial genome of *** this study,we compare two sequencing methods in Epeorus sp.(Ephemeroptera, Insecta).We found the NGS is more convenient,cheaper and *** complete mtDNA genome is 15,338 bp long and contains 38 genes,and all genes are similar to Parafronurus youi with extral *** nucleotide composition of the genome is highly A+T biased(64.7%).A total of 1771 bp of non-coding sequences were observed at 31 *** overall A+T content of control region(72.7%),which is similar to other studied *** PCGs in the mitochondrial genome of Epeorus *** with the ATD start codon(ATG,ATC,ATT or ATA) and GTG,and terminated with TAA or TAG.

关键词： Next-generation sequencing Method comparison Epeorus sp. mitochondrial genome

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Effects of SHANK2 gene knockdown on early neural development...

基于细观层次混凝土骨料模型生成方法研究分析

中国神经科学学会第十二届全国学术会议

作者： Chen Xiaoxia Huang Zhonghai Kang Sai yu panpan Shi Lingling Guangdong-Hongkong-Macau Institute of CNS Regeneration Joint International Research Laboratory of CNS Regeneration Ministry of Education of PRC Jinan University Co-innovation Center of Neuroregeneration Nantong University

OBJECTIVE: To investigate the effect of SHANK2 knockdown on the early morphological development of neurons derived from human induced pluripotent stem cell（iPSCs）. METHODS: Induced pluripotent stem cells（iPSCs） were differentiated into neural progenitor cells（NPC） and NPCs were differentiated into neurons. The models were divided into sh Control group（infected with control Lenti virus packed with a red fluorescent protein expression sequence） and sh SHANK2 group（infected with SHANK2 Lenti virus packed with a red fluorescent protein expression sequence）. The soma area, growth cone area, neurite length and branches number of neurons were analyzed by immunohistochemical method on selected differentiation days（Day3, Day6, Day9, Day12, and Day15）. RESULTS:（1） The model with NPC was generated in vitro significantly.（2） Compared with control, SHANK2 gene knockdown resulted in the decrease of soma area at Day9、Day12、Day15.（3） Compared with control, the number of total branches, primary branches, and primary branches with SHANK2 gene knockdown were reduced, and the length of the neurite, axon, and dendrite were reduced at Day6、Day9、Day12、Day15.（4） Compared with control, SHANK2 gene knockdown resulted in the decrease of three subtypes of growth cone area bluntended（collapsed with no visible filopodia or lamellipodia）, filopodial（growth cones with numerous filopodia and a small or absent lamellipodial veil）, or lamellipodial（well spread growth cones with elaborate lamellipodia） at Day6 、 Day9 、 Day12 、 Day15. CONCLUSION: The early development of neurons were affected by SHANK2 gene knockdown, leading to abnormal neuronal morphology development.

关键词： SHANK2 gene Induced pluripotent stem cells Gene knockdown Neuronal morphology

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材料导报 2016年第17期30卷 102-108页

作者：于江田盼盼秦拥军新疆大学建筑工程学院乌鲁木齐830047

混凝土细观数值模拟分析成为了一种有前景替代大量宏观试验的方式,但目前数值模拟中缺少成熟的细观力学模型。重点介绍了混凝土的3种细观力学模型,即随机骨料模型、数字图像模型和参数等效化单元模型,详细说明这3种细观骨料模型的生成... 详细信息

混凝土细观数值模拟分析成为了一种有前景替代大量宏观试验的方式,但目前数值模拟中缺少成熟的细观力学模型。重点介绍了混凝土的3种细观力学模型,即随机骨料模型、数字图像模型和参数等效化单元模型,详细说明这3种细观骨料模型的生成特点、关键问题和研究成果,对比分析3种细观模型的生成效率、网格化分、计算效率和精确度等,并提出了在这方面有待进一步研究的问题。

关键词：细观层次混凝土模型

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创新要素流动、税制结构与制造业高质量发展

工业技术经济 2023年第7期42卷 123-132页

作者：田时中余盼盼安徽大学经济学院合肥230601

探究创新要素流动和税制结构对制造业高质量发展的作用机理及影响效应,有利于加快实现“制造强国”目标。本文基于多维指标体系和中国30个省(区、市)2009~2021年面板数据,对中国制造业高质量发展指数进行熵值处理,构建面板Tobit模型进... 详细信息

探究创新要素流动和税制结构对制造业高质量发展的作用机理及影响效应,有利于加快实现“制造强国”目标。本文基于多维指标体系和中国30个省(区、市)2009~2021年面板数据,对中国制造业高质量发展指数进行熵值处理,构建面板Tobit模型进行实证检验,结果发现:(1)创新要素流动对制造业高质量发展有显著的促进作用;提升直接税整体比重有利于制造业高质量发展;(2)创新要素流动和税制结构的交互作用并不能提升制造业高质量发展水平;(3)绿色技术创新水平的提升,能够显著增强R&D人员流动对制造业高质量发展的促进作用,但并不能增强R&D资本流动对制造业高质量发展的促进作用;扩大企业所得税规模、降低增值税规模均有利于发挥税制结构对制造业高质量发展的积极影响。以上结论对新发展阶段提升中国制造业高质量发展水平具有重要的政策启示。

关键词：创新要素流动税制结构制造业高质量发展熵值法 Tobit模型