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4种轮作模式下长期连作烟田土壤微生态的响应特征

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中国农业科技导报 2024年第3期26卷 174-187页

作者：周旭东韩天华申云鑫施竹凤贺彪杨明英裴卫华何永宏杨佩文云南省农业科学院农业环境资源研究所昆明650205 云南省烟草公司玉溪市公司澄江分公司云南澄江652500 云南省烟草公司丽江市公司云南丽江674100

为探究烤烟与不同作物轮作对长期连作植烟土壤质量调控的效果,以连作10年以上的植烟田块为对照(CK),设置大麦-烤烟(YCDM)、大蒜-烤烟(YCDS)、油菜-烤烟(YCYC)和蚕豆-烤烟(YCCD)共4种不同作物与烤烟轮作,分析不同轮作处理下土壤的理化性... 详细信息

为探究烤烟与不同作物轮作对长期连作植烟土壤质量调控的效果,以连作10年以上的植烟田块为对照(CK),设置大麦-烤烟(YCDM)、大蒜-烤烟(YCDS)、油菜-烤烟(YCYC)和蚕豆-烤烟(YCCD)共4种不同作物与烤烟轮作,分析不同轮作处理下土壤的理化性质、酶活性及微生物群落结构。基于相关性分析和冗余分析,解析土壤理化性质和酶活性与微生物群落结构间的关系。结果表明,轮作处理的土壤容重较CK降低26.58%~30.29%,总孔隙度增加21.13%~48.26%;其中烤烟-蚕豆(YCCD)轮作模式下土壤的pH、水解氮(available nitrogen,AN)、有效磷(available phosphorus,AP)和速效钾(available potassium,AK)含量较CK显著提高11.84%、30.57%、6.42%和41.51%。轮作处理的土壤过氧化氢酶(catalase,CAT)、蔗糖酶(invertase,INV)、脲酶(urease,URE)和酸性磷酸酶(acid phosphatase,ACP)的活性较CK分别提高16.81%~42.35%、38.09%~51.48%、7.69%~64.29%和5.82%~76.33%,蚕豆-烤烟(YCCD)轮作模式的提升效果最为显著。高通量测序结果表明,轮作处理下细菌的OTUs数量显著高于CK。α多样性显示,不同轮作处理间的细菌群落丰富度和多样性差异显著;真菌群落的丰富度差异显著,而多样性差异不显著。β多样性分析显示,不同轮作处理之间土壤真菌群落差异较小,细菌群落差异较大。其中,蚕豆-烤烟(YCCD)轮作处理的细菌、真菌群落与CK差异较大。在门水平,烤烟-蚕豆(YCCD)轮作使酸杆菌门(Acidobacteria)和放线菌门(Actinobacteria)的相对丰度增加,子囊菌门(Ascomycota)的相对丰度减少。相关性分析和冗余分析表明,URE、AN、AK和pH是影响土壤微生物群落结构的关键因子。综上所述,烤烟与其他作物合理轮作可提高土壤速效养分含量和土壤酶活性,调节土壤微生物群落结构,消减烤烟连作障碍,最终达到烤烟稳产、增产的目的。

关键词：植烟土壤轮作土壤理化性质土壤酶活性微生物群落

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A Novel Method to Enhance the Inversion Speed and Precision of the NMR T_(2) Spectrum by the TSVD Based Linearized Bregman Iteration

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Computer Modeling in Engineering & Sciences 2023年第9期136卷 2451-2463页

作者： Yiguo Chen Congjun Feng yonghong he Zhijun Chen Xiaowei Fan Chao Wang Xinmin Ge State Key Laboratory of Continental Dynamics Northwest UniversityXi’an710069China Department of Geology Northwest UniversityXi’an710069China Reseach Institute of Shaanxi Yanchang Petroleum(Group)Co. Ltd.Xi’an710075China School of Geosciences China University of PetroleumQingdao266580China

The low-field nuclear magnetic resonance(NMR)technique has been used to probe the pore size distribution and the fluid composition in geophysical prospecting and related ***,the speed and accuracy of the existing numerical inversion methods are still challenging due to the ill-posed nature of the first kind Fredholm integral equation and the contamination of the *** paper proposes a novel inversion algorithmto accelerate the convergence and enhance the precision using empirical truncated singular value decompositions(TSVD)and the linearized Bregman *** L1 penalty term is applied to construct the objective function,and then the linearized Bregman iteration is utilized to obtain fast *** reduce the complexity of the computation,empirical TSVD is proposed to compress the kernel matrix and determine the appropriate truncated *** novel inversion method is validated using numerical *** results indicate that the proposed novel method is significantly efficient and can achieve quick and effective data solutions with low signal-to-noise ratios.

关键词： Low field nuclear magnetic resonance linearized bregman iteration truncated singular value decomposition numerical simulations

Engineering organoid microfluidic system for biomedical and health engineering:A review

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Chinese Journal of Chemical Engineering 2021年第2期34卷 244-254页

作者： Yifan Xing Junyu Liu Xiaojie Guo Haipeng Liu Wen Zeng Yi Wang Chong Zhang Yuan Lu Dong he Shaohua Ma yonghong he Xin-Hui Xing Key Laboratory for Industrial Biocatalysis Ministry of Education of ChinaDepartment of Chemical EngineeringTsinghua UniversityBeijing 100084China Centre for Synthetic and Systems Biology Tsinghua UniversityBeijing 100084China School of Chemical Engineering and Light Industry Guangdong University of TechnologyGuangzhou 510006China Institute of Biopharmaceutical and health Engineering Tsinghua Shenzhen International Graduate SchoolShenzhen 518055China Institute of Biomedical health Technology and Engineering Shenzhen Bay LaboratoryShenzhen 518055China

In recent years,organoid technology,i.e.,in vitro three-dimensional(3D)tissue culture,has attracted increasing attention in biomedical *** are cell complexes induced by differentiation of stem cells or organ-progenitor cells in vitro using 3D culture *** can replicate the key structural and functional characteristics of the target organs in *** the opening up of this new field of health engineering,there is a need for engineering-system approaches to the production,control,and quantitative analysis of organoids and their *** organoid technology has limitations,including lack of physical and chemical microenvironment control,high heterogeneity,complex manual operation,imperfect nutritional supply system,and lack of feasible online analytical technology for the *** introduction of microfluidic chip technology into organoids has overcome many of these limitations and greatly expanded the scope of *** organoid microfluidic system has become an interdisciplinary field in biomedical and health *** this review,we summarize the development and culture system of organoids,discuss how microfluidic technology has been used to solve the main technical challenges in organoid research and development,and point out new opportunities and prospects for applications of organoid microfluidic system in drug development and screening,food safety,precision medicine,and other biomedical and health engineering fields.

关键词： Organoids Stem cell Culture system Microfluidics Biomedical engineering Human health

Enrichment of Tight oil in Yanchang Formation in Ordos Basin and Effective Development Technology

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Acta Geologica Sinica(English Edition) 2015年第A1期89卷 431-432页

作者： YU Jian he yonghong WANG Xiujuan Department of reservoir evaluation Changqing Oilfield CompanyPetrochina

Tight oil in Yanchang formation, late Triassic, formed in fairly deep lake to deep lake sedimentary facies, is a kind of tight sandstone oil. With typical cases of that in Chang7 oil-bearing layer and Chang6 oil-beari... 详细信息

关键词： PETROLEUM reservoirs TECHNOLOGICAL innovations PETROLEUM prospecting PETROLEUM geology BASINS (Geology) CHINA

光谱CT多参数成像在进展期胃癌术前新辅助化疗疗效评估中的应用价值

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实用医学影像杂志 2024年第4期25卷 249-252页

作者：何永红吴景强邱清香游其奎福建医科大学附属龙岩第一医院放射科 361000

目的探讨双层探测器光谱CT获得的各光谱参数在进展期胃癌术前化疗前后的数据变化,并评估各参数在进展期胃癌化疗疗效中临床价值。方法回顾性分析2021年3月至2024年2月我院经病理证实的28例经短期(4周期)化疗好转[包括实体瘤疗效评价标... 详细信息

目的探讨双层探测器光谱CT获得的各光谱参数在进展期胃癌术前化疗前后的数据变化,并评估各参数在进展期胃癌化疗疗效中临床价值。方法回顾性分析2021年3月至2024年2月我院经病理证实的28例经短期(4周期)化疗好转[包括实体瘤疗效评价标准(RECIST 1.1标准)完全缓解(CR)、部分缓解(PR)]的进展期胃癌患者术前及短期化疗后光谱CT图像,测量及计算动脉期、静脉期所有光谱参数,包括标准化碘密度值(NIC)、光谱曲线斜率[K(40~100 keV)]值及有效原子系数(Zeff),比较2组间各光谱参数的差异,并使用受试者工作特征曲线(ROC)分析灵敏度、特异度以衡量每个参数的诊断效能,比较各AUC(曲线下面积)的差异。结果 (1)动脉期及静脉期NIC、K(40~100 keV)、Zeff值在治疗前均高于治疗后,且各参数治疗前后数值差异均有统计学意义(P<0.05)。(2)ROC曲线进一步分析NIC、K(40~100 keV)、Zeff联合应用诊断进展期胃癌治疗疗效效能。动脉期NIC、K(40~100 keV)、Zeff曲线下面积(AUC)分别为:0.772、0.759、0.804;NIC、K(40~100 keV)、Zeff灵敏度和特异度分别为:71%和78%,61%和86%,75%和75%;静脉期NIC、K (40~100keV)、Zeff曲线下面积(AUC)分别为:0.810、0.779、0.895;NIC、K(40~100 keV)、Zeff灵敏度和特异度分别为:96%和54%,79%和71%,75%和89%。结论光谱CT NIC、K(40~100 keV)、Zeff参数可作为评估进展期胃癌术前化疗疗效的可靠标记物,联合应用更能提高诊断的准确率。

关键词：光谱CT 多参数成像胃癌化疗疗效

Adsorption of trichlorphon on phyllosilicate minerals:Effect of low-molecular-weight organic acids

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Pedosphere 2024年第1期34卷 78-87页

作者： Hongfeng CheN Zhouyang he Mingxia HOU Cilai TANG yonghong WU College of Hydraulic&Environmental Engineering China Three Gorges UniversityYichang 443002China Hubei Field Observation and Scientific Research Stations for Water Ecosystem in Three Gorges Reservoir China Three Gorges UniversityYichang 443002China Engineering Research Center of Eco-Environment in Three Gorges Reservoir Region China Three Gorges UniversityYichang 443002China State Key Laboratory of Soi and Sustainable Agriculture Institute of Soil SciencesChinese Academy of SciencesNanjing 210008China

The use of trichlorphon in large quantities causes a large number of organic pollutants to enter water, sediments, and soils. Phyllosilicate minerals are considered effective adsorbents for organic pollutants. However, the adsorption behavior of organic pollutants on soil minerals affected by low-molecular-weight organic acids(LMWOAs) is not fully understood. In this study, the effect of LMWOAs on the adsorption behavior of trichlorphon on phyllosilicate minerals was investigated using a combination of adsorption measurements and molecular spectroscopic techniques(attenuated total reflection-Fourier transform infrared spectroscopy(ATR-FTIR) and X-ray photoelectron spectroscopy(XPS)). The adsorption of trichlorphon onto kaolinite(KAO) and montmorillonite(MON) was suppressed by increasing pH, indicating that electrostatic interaction played a key role in adsorption, especially at low pH. In the presence of citric acid(CA), there was an obvious promotion of trichlorphon adsorption on KAO and MON. In the presence of oxalic acid(OA), the adsorption of trichlorphon on KAO was promoted, whereas the adsorption on MON was inhibited, especially at pH 4.0. The presence of CA and OA increased the adsorption by increasing the exposure of hydrophobic sites of KAO and MON. The results from ATR-FTIR and XPS also indicated that the hydrophobic Si–O sites of phyllosilicate minerals were the preferred adsorption sites for trichlorphon in the presence of CA and OA, probably driven by the hydrophobic effect. However, the weak effect of OA on MON caused an increase in the electrostatic repulsion between MON and trichlorphon molecules, thus inhibiting adsorption. This study is significant for a deeper understanding of self-purification of soil and sediment systems in the presence of organic pollutants.

关键词： adsorption behavior citric acid hydrophobic effect organic pesticide organic pollutant organochlorine oxalic acid

A saccharides sensor developed by symmetrical optical waveguide-based surface plasmon resonance

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Journal of Innovative Optical health Sciences 2015年第2期8卷 19-25页

作者： Ang Li Zhouyi Guo Qing Peng Chan Du Xida Han Le Liu Jun Guo yonghong he Yanhong Ji MOE Key Laboratory of Laser Life Science&SATCM Third Grade Laboratory of Chinese Medicine and Photonics Technology College of Biophotonics South China Normal University Guangzhou 510631P.R.China Department of Ophthalmology Xinhua Hospital Shanghai Jiaotong University School of Medicine Shanghai 200092P.R.China Shenzhen Key Laboratory for Minimal Invasive Medical Technologies Graduate School at Shenzhen Tsinghua University Shenzhen 518055P.R.China MOE Key Laboratory of Laser Life Science College of Biophotonics South China Normal University Guangzhou 510631P.R.China

We proposed a new saccharides sensor developed by symmetrical optical waveguide(SOW)-based surface plasmon resonance(SPR).This unique MgF_(2)/Au/MgF_(2)/Analyte film structure results in longer suface plasmon wave(SPW)propagation lengths and depths,leading to an increment of *** this paper,we managed to decorate the dielectric interface(MgF_(2) layer)by depositing a thin polydopamine film as surface adherent that provides a platform for secondary reactions with the probe molecule.3-Aminophenylboronic acid(3-PBA)is chosen to be the saccharides sense probe molecule in the present *** aqueous huumnor of Diabetes and Cataract patient whose blood glucose level is normal are analyzed and the results dermonstrated that this sensor shows great potential in monitoring the blood siugar and can be adapted in the field of biological monitoring in the future.

关键词： Saccharides sensor surface plasmon resonance symmetrical optical waveguide 3-Aminophenylboronic acid dopamine

鄂尔多斯盆地延安地区山西组山2段浅水曲流河三角洲沉积模拟实验研究

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沉积学报 2024年第5期42卷 1685-1698页

作者：曹军乔向阳贺永红周进松杜永慧王玺童田坤赵子丹朱耿博仑罗顺社陕西延长石油(集团)有限责任公司西安710065 长江大学地球科学学院武汉430100

【目的】勘探实践表明,鄂尔多斯盆地东南部延安气田主力产气层山西组山2段砂体呈狭长条带状分布,与北部各大气田上古生界砂体大面积连片分布存在显著差异,厘清山西组山2段砂体成因机理对理解该区砂体分布规律具有重要意义。【方法】以... 详细信息

【目的】勘探实践表明,鄂尔多斯盆地东南部延安气田主力产气层山西组山2段砂体呈狭长条带状分布,与北部各大气田上古生界砂体大面积连片分布存在显著差异,厘清山西组山2段砂体成因机理对理解该区砂体分布规律具有重要意义。【方法】以盆地东南部山西组山2段为地质原型,利用水槽沉积模拟实验探讨其砂体形成演化过程及控制因素。【结果与结论】平缓坡度与低水流强度是山2段分流河道呈低弯度曲流态的重要因素,水平面的变幅速率是控制山2段砂体纵向延伸的关键因素,砂体的大小及长宽比受岸线及流量变化控制,砂体整体的延伸距离及长宽比分别与水退的幅度和速率成正比。山西组沉积期,在水平面大幅度下降、岸线向湖区长距离迁移背景下,曲流河三角洲前缘砂体延伸较远距离;砂体叠置样式具有“垂向叠置”、“垂向切割”和“侧向迁移”的特征。该认识对山西组砂体分布预测和寻找优质储层具有重要的指导意义。

关键词：水槽模拟实验浅水曲流河三角洲控砂机制山2段鄂尔多斯盆地

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三株酚酸降解菌的筛选与鉴定及其生物活性

微生物学通报 2024年第6期51卷 2193-2214页

作者：廖永琴王楠申云鑫唐加菜施竹凤矣小鹏冯路遥李铭刚何永宏杨佩文云南农业大学植物保护学院云南昆明650000 云南省农业科学院农业环境资源研究所云南昆明650205 云南大学资源植物研究院云南昆明650091

【背景】常见作物连续种植后会导致根系分泌酚酸类自毒物质,该类物质是引起土传病害和连作障碍频发的主要因素。【目的】探讨利用有益微生物降解作物根系分泌物苯甲酸,缓解作物土传病害发生,并储备有益菌种资源。【方法】通过以苯甲酸... 详细信息

【背景】常见作物连续种植后会导致根系分泌酚酸类自毒物质,该类物质是引起土传病害和连作障碍频发的主要因素。【目的】探讨利用有益微生物降解作物根系分泌物苯甲酸,缓解作物土传病害发生,并储备有益菌种资源。【方法】通过以苯甲酸为唯一碳源的无机盐培养基筛选无量山菌株资源,结合高效液相色谱仪测定酚酸降解菌株对苯甲酸的降解率;利用平板对峙试验,并基于缓解苯甲酸抑制番茄种子萌发试验及番茄幼苗促生长和对番茄枯萎病防效试验验证菌株的生物活性;结合形态学、生理生化和16S rRNA基因序列对菌株进行分类学鉴定。【结果】从无量山筛选得到4株放线菌和3株细菌具有酚酸降解能力,酚酸降解菌株在摇瓶培养72 h时对苯甲酸降解率分别为100%、99.83%、99.89%、99.87%、64.91%、56.92%和49.33%;其中2株放线菌YNK-FS0018、YNK-FS0019和一株细菌YNK-FB0022能较好地利用苯甲酸。番茄种子萌发试验结果表明菌株YNK-FS0018、YNK-FS0019和YNK-FB0022均可以缓解苯甲酸对种子萌发的抑制,促进种子萌发,萌发率分别为73%、73%和97%,并促进种子根生长,平均根长为13.31、13.04和14.56 cm;平板对峙试验结果表明菌株YNK-FS0018、YNK-FS0019和YNK-FB0022均对病原菌有广谱抗性;抗生素基因检测结果为菌株YNK-FS0018、YNK-FS0019具有PKSII、NRPS基因,菌株YNK-FB0022具有fenD、bioA、yndJ、ysnE、ituC、sboA和srfAB基因;盆栽试验结果显示菌株YNK-FS0018对番茄株高、地上鲜重、地上干重、根重、根长和茎粗有促生作用,各项分别增加53.40%、67.50%、57.50%、73.50%、77.28%和9.40%;菌株YNK-FS0019处理的株高、地上鲜重、地上干重、根重、根长和茎粗分别增加23%、40.90%、14.00%、57.00%、26.30%和16.80%;菌株YNK-FB0022处理的株高、地上鲜重、地上干重、根重、根长和茎粗分别增加24.50%、44.70%、26.40%、75.10%、98.00%和9.00%;3株菌对番茄枯萎病的防效分别为87.10%、74.18%和80.65%;结合形态学和分子生物学鉴定,菌株YNK-FS0018为稻瘟霉素链霉菌(Streptomyces blastmyceticus,GenBank登录号为OR523286),YNK-FS0019为白黄链霉菌(Streptomyces alboflavus,GenBank登录号为OR523289),YNK-FB0022为耐盐芽孢杆菌(Bacillus halotolerans,GenBank登录号为OR523290);此外,菌株均有解有机磷、溶锌、分泌铁载体、水解淀粉酶和水解蛋白酶的能力。【结论】三株酚酸降解菌具有解有机磷、溶锌、分泌铁载体、水解淀粉酶和水解蛋白酶的能力,对番茄幼苗株高、茎粗、根长、根重、地上部分鲜重和干重等农艺性状具有显著促进作用,对番茄枯萎病防效较好,可为土传病害的生物防治上提供微生物资源。

关键词：苯甲酸酚酸降解菌促生长拮抗番茄枯萎病