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Induction of Apoptosis by L-NMMA,via FKHRL1/ROCK Pathway in Human Gastric Cancer Cells

Biomedical and Environmental Sciences 2006年第4期19卷 285-291页

作者： yong-zhong wang zhen-qing feng Department of Pathology Nanjing Medical University Nanjing 210029 Jiangsu China

Objective To investigate the apoptosis-inducing effect of endogenous nitric oxide （NO） suppression in gastric cancer cells and its mechanisms. Methods Apoptosis of gastric cancer cells was detected by flow cytometry. Expression of phosphorylated FKHRL1 （thr-32, ser-253） and FKHRL1 in gastric cancer cells was analyzed using Western blotting. Immunofluorescence assay was performed to localize the intracellular phosphorylated FKHRL1 （thr-32, ser-253） and FKHRL 1. Transfection of FKHRL1-HA wild type and mutant FKHRL1-HA T32A constructs was performed by lipofectamine plus reagent. NO generation was determined by Griess reaction. Results Gastric cancer cells were significantly apoptotic after treatment with NG-monomethyl-L-arginine （L-NMMA, a nitric oxide synthase inhibitor）, compared with the control （P〈0.01）. The apoptosis of gastric cancer cells induced by L-NMMA was dose-dependent and time-independent. However, the Z-DEVD-fmk, a caspase-3, 6, 7, 8, l0 inhibitor, did not prevent the apoptosis. The immunofluorescence assays showed that FKHRL1 protein was strongly expressed in the nucleu and p-FKHRL1 thr-32 protein was ：strongly expressed in the cytoplasm of SGC-7901 cells when endogenous nitric oxide generation was blocked by L-NMMA, but no change in FKHRL1 ser-253 phosphorylation. Nevertheless, ROCK protein was strongly expressed in p-FKHRL1 thr-32-positive SGC-7901 cells. The wortmannin, an inhibitor of phosphoinositol-3-OH kinase （PI3K）, did not block the phosphorylated FKHRL1 thr-32 protein induced by L-NMMA. However, Y-27632, a specific inhibitor of the protein kinase ROCK, significantly blocked apoptosis induced by phosphorylated FKHRL1 thr-32 （P〈0.01）, which was mediated by L-NMMA. A significant decrease in NO generation （P〈0.01） and a significant increase in apoptosis （P〈0.01） were observed when FKHRL1-HA wild-type cells were transfected, which caused increased FKHRL1 thr-32 phosphorylation. Conclusions L-NMMA triggers gastric carcinoma cell apoptosis, possibly

关键词： Gastric carcinoma Nitric oxide FKHRL1 Apoptosis Signaling

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Design and catalytic atroposelective synthesis of axially chiral isochromenone-indoles

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Science China Chemistry 2022年第10期65卷 1929-1937页

作者： qing-qing Hang Shu-Fang Wu Shuang Yang Xue wang zhen zhong Yu-Chen Zhang feng Shi Research Center of Chiral Functional Heterocycles School of Chemistry&Materials ScienceJiangsu Normal UniversityXuzhou 221116China School of Petrochemical Engineering Changzhou UniversityChangzhou 213164China

The catalytic atroposelective synthesis of axially chiral isochromenone-indoles has been established by the strategy of designing homophthalic anhydride-based indole derivatives as a new type of indole-based platform molecules for dynamic kinetic *** this strategy,a wide range of axially chiral isochromenone-indoles were synthesized in high yields with excellent enantioselectivities(up to 98% yield,97% ee) via the catalytic asymmetric sulfonylation reaction of homophthalic anhydridebased indole derivatives with aryl sulfonyl chlorides under the catalysis of chiral quaternary ammonium salt as a phase-transfer catalyst.

关键词： asymmetric organocatalysis axial chirality atroposelectivity enantioselectivity indole

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As-cast structure of DC casting 7075 aluminum alloy obtained under dual-frequency electromagnetic field

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International Journal of Minerals,Metallurgy and Materials 2014年第2期21卷 150-154页

作者： Zhi-hao Zhao zhen Xu Gao-song wang qing-feng Zhu Jian-zhong Cui The Key Laboratory of the Ministry of Education of China for Electromagnetic Processing of Materials Northeastern University

We have experimentally determined the as-cast structures of semi-continuous casting 7075 aluminum alloy obtained in the pres-ence of dual-frequency electromagnetic field. Results suggest that the use of dual-frequency electromagnetic field during the semi-continuous casting process of 7075 aluminum alloy ingots reduces the thickness of the surface segregation layer, increases the height of the melt menis-cus, enhances the surface quality of the ingot, and changes the surface morphology of the melt pool. Moreover, low-frequency electromag-netic field was found to show the most obvious influence on improving the as-cast structure because of its high permeability in conductors.

关键词： electromagnetic fields: semi-continuous casting aluminum alloys as-cast structure

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Perylene Diimide Based Isomeric Conjugated Polymers as Efficient Electron Acceptors for All-polymer Solar Cells

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Chinese Journal of Polymer Science 2019年第1期37卷 18-27,1-5页

作者： Xiao-Cheng Liu qing-Wu Yin Zhi-Cheng Hu zhen-feng wang Fei Huang yong Cao State Key Laboratory of Luminescent Materials and Devices School of Materials Science and Engineering South China University of Technology South China Institute of Collaborative Innovation

We present here a series of perylene diimide(PDI)based isomeric conjugated polymers for the application as efficient electron acceptors in all-polymer solar cells(all-PSCs).By copolymerizing PDI monomers with 1,4-diethynylbenzene(para-linkage)and 1,3-diethynylbenzene(meta-linkage),isomeric PDI based conjugated polymers with parallel and non-parallel PDI units inside backbones were *** was found that para-linked conjugated polymer(PA)showed better solubility,strongerπ-πstacking,more favorable blend morphology,and better photovoltaic performance than those of meta-linked conjugated polymers(PM)*** based on PTB7-Th:PA(PTB7-Th:poly{4,8-bis[5-(2-ethylhexyl)-thiophen-2-yl]benzo[1,2-b:4,5-b']dithiophene-2,6-diyl-alt-3-fluoro-2-[(2-ethylhexyl)-carbonyl]thieno[3,4-b]thiophene-4,6-diyl})showed significantly enhanced photovoltaic performance than that of PTB7-Th:MA(3.29%versus 0.92%).Moreover,the photovoltaic performance of these polymeric acceptors could be further improved via a terpolymeric *** copolymerizing a small amount of meta-linkages into PA,the optimized terpolymeric acceptors enabled to enhance photovoltaic performance with improved the short-circuit current density(Jsc)and fill factor(FF),resulting in an improved power conversion efficiency(PCE)of 4.03%.

关键词： Isomeric conjugated polymers All-polymer solar cells Electron acceptors Perylene diimide

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Effects of strain rate on austenite stability and mechanical properties in a 5Mn steel

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Journal of Iron and Steel Research International 2022年第2期29卷 316-326页

作者： yong-gang Yang wang-zhong Mu Xiao-qing Li Hai-tao Jiang Mai wang zhen-li Mi Xin-ping Mao Beijing Advanced Innovation Center for Materials Genome Engineering National Engineering Research Center for Advanced Rolling TechnologyUniversity of Science and Technology BeijingBeijing100083China Department of Materials Science and Engineering KTH Royal Institute of TechnologyStockholm10044Sweden Department of Mechanical Engineering The University of Hong KongHong Kong999077China

The austenite stability and the mechanical properties in a typical medium Mn grade steel,i.e.,5Mn steel,were investigated under a wide range of strain rates through the combination of experimental and theoretical *** obtained results indicate that austenite is more stable at a high strain rate,which is due to the suppression of the austenite to martensite *** suppression is attributed to the increased stacking fault energy and the high deformation energy ***,the suppression of martensitic transformation also leads to the decrease in the ultimate tensile strength and the uniform *** to the increase in an adiabatic heating temperature,an increase in the uniform elongation is acquired at a high strain *** obtained fundamental study results shed light on a wide application of the medium Mn steel under different strain rate conditions.

关键词： Medium Mn steel Austenite stability Microstructure Mechanical property Strain rate

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The Role of Processing Solvent on Morphology Optimization for Slot-Die Printed Organic Photovoltaics

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Chinese Journal of Polymer Science 2023年第6期41卷 842-850,I0005页

作者： Lei wang Jun-Zhe Zhan Wen-Kai zhong Lei Zhu Guan-qing Zhou Tian-Yu Hao Ye-Cheng Zou zhen-Hua wang Gang Wei yong-Ming Zhang feng Liu School of Physics and Astronomy Shanghai Jiao Tong UniversityShanghai 200240China Frontiers Science Center for Transformative Molecules Center of Hydrogen ScienceSchool of Chemistry and Chemical EngineeringShanghai Jiao Tong UniversityShanghai200240China State Key Laboratory of Fluorinated Functional Membrane Materials and Dongyue Future Hydrogen Energy Materials Company Zibo 256401China

The morphology manipulation of the active layers is important for improving the performance of organic photovoltaics(OPVs).The choice of processing solvent has great impact on the crystallization and phase separation during film formation,since solvent properties,including solvent effect on molecular crystallization,boiling point,and interaction parameters,can directly change the evolution pathways associated with thermodynamics and ***,revealing the underlying solvent-regulated morphology mechanism is potential to provide guiding strategies for device *** this study,chloroform,chlorobenzene,and toluene are used to process PM6:Y6 blends by slot-die printing to fabricate OPV *** chloroform printed film forms a fibrillar network morphology with enhanced crystallization,facilitating exciton dissociation,charge transport and extraction,resulting in an optimal power conversion efficiency of 16.22%.However,the addition of the additive chloronaphthalene in chloroform solution leads to over-crystallization of Y6,and thus,increasing domain size that exceeds the exciton diffusion length,resulting in lower device *** addition,both the chlorobenzene and toluene suppress the crystallization of Y6,which drastically decreased short-circuit current and fill *** results demonstrate the important role of processing solvent in dictating film morphology,which critically connects with the resultant printed OPV performance.

关键词： crystallization chloroform film

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The effects of calcium combined with chitosan amendment on the bioavailability of exogenous Pb in calcareous soil

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Journal of Integrative Agriculture 2020年第5期19卷 1375-1386页

作者： CHANG Hui-qing wang Qi-zhen LI Zhao-jun WU Jie XU Xiao-feng SHI Zhao-yong Henan University of Science and Technology Luoyang 471000P.R.China Key Laboratory of Plant Nutrition and Fertilizer Ministry of Agriculture and Rural Affairs/China-New Zealand Joint Laboratory for Soil Molecular EcologyInstitute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081P.R.China

Lead(Pb) in soil may accumulate in crops and enter the human body. This study aimed to understand the speciation transformation and accumulation characteristics of exogenous Pb in calcareous soil with or without the application of soil amendments. Field experiments with continuous maize cultivation have been carried out for two years. The results showed that the contents of total Pb were slightly lower in 2016 than in 2015 for the same treatments;however, no significant difference between the years was observed. Soil Pb existed mainly in the residual fraction without exogenous Pb addition, and its proportion was more than 33% of the total soil Pb in the control and Ca treatments. When Pb was added to calcareous soil, Pb existed largely in the oxidizable and reducible fractions during the two-year experimental period, and those fractions made up over 83% of the total Pb. The proportion of the water-soluble Pb, regardless of the addition of Pb, was the lowest and was less than 0.0019% in all treatments, but the addition of Ca and chitosan amendments reduced the water-soluble and exchangeable Pb contents. The Pb content in different parts of maize followed the order root>leaf>stem>grain during the experimental period. Although maize had low transfer and enrichment factors in calcareous soil, which make the Pb content in the grain show no significant difference among the five treatments in the same year, adding Ca and chitosan to calcareous soil can reduce the Pb contents of maize, especially reduce the Pb contents of root, stem and leaf. Therefore, the addition of calcium and chitosan is an effective strategy for reducing Pb availability in calcareous soils.

关键词： exogenous Pb maize calcareous soil speciation transformation amendments

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“Charging”the cigarette butt:heteroatomic porous carbon nanosheets with edge-induced topological defects for enhanced oxygen evolution performance

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Frontiers of Chemical Science and Engineering 2023年第11期17卷 1755-1764页

作者： qing-Hui Kong Xian-Wei Lv Jin-Tao Ren Hao-Yu wang Xin-Lian Song feng Xu zhong-yong Yuan School of Materials Science and Engineering Smart Sensing Interdisciplinary Science CenterNankai UniversityTianjin 300350China Tianjin Workstation Technology Center of Shanghai Tobacco Group Co.Ltd.Tianjin 300163China Key Laboratory of Advanced Energy Materials Chemistry(Ministry of Education) Nankai UniversityTianjin 300071China

Owing to the complexity of electron transfer pathways,the sluggish oxygen evolution reaction process is defined as the bottleneck for the practical application of Zn-air *** this effort,metal nanoparticles(Co,Ni,Fe,etc.)encapsulated within nitrogen-doped carbon materials with abundant edge sites were synthesized by one-step pyrolysis treatment using cigarette butts as raw materials,which can drastically accelerate the overall rate of oxygen evolution reaction by facilitating the adsorption of oxygenated intermediates by the edge-induced topological *** prepared catalyst of nitrogen-doped carbon porous nanosheets loaded with Co nanoparticles(Co@NC-500)exhibits enhanced catalytic activity toward oxygen evolution reaction,with a low overpotential of 350 mV at the current density of 10 mA·cm^(-2).Furthermore,the Zn-air battery assembled with Co@NC-500 catalyst demonstrates a desirable performance affording an open-circuit potential of 1.336 V and power density of 33.6 mW·cm^(-2),indicating considerable practical application potential.

关键词： oxygen evolution reaction porous carbon nanosheets Co nanoparticles edge-induced topological defects Zn-air batteries

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Encoding and display technologies for combinatorial libraries in drug discovery:The coming of age from biology to therapy

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Acta Pharmaceutica Sinica B 2024年第8期14卷 3362-3384页

作者： Yu Fan Ruibing feng Xinya Zhang zhen-Liang wang feng Xiong Shuihua Zhang Zhang-feng zhong Hua Yu qing-Wen Zhang Zhang Zhang Yitao wang Guodong Li Macao Centre for Research and Development in Chinese Medicine State Key Laboratory of Quality Research in Chinese MedicineInstitute of Chinese Medical SciencesUniversity of MacaoMacao SAR 999078China State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacao SAR 999078China International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development Ministry of Education(MoE)of People’s Republic of ChinaCollege of PharmacyJinan UniversityGuangzhou 510632China Zhuhai UM Science and Technology Research Institute Zhuhai 519031China Department of Pharmacy Guangzhou Red Cross HospitalFaculty of Medical ScienceJinan UniversityGuangzhou 510632China Geriatric Medicine First People’s Hospital of XinXiang and the Fifth Affiliated Hospital of Xinxiang Medical CollegeXinxiang 453100China Shenzhen Innovation Center for Small Molecule Drug Discovery Co. Ltd.Shenzhen 518000China

Drug discovery is a sophisticated process that incorporates scientific innovations and cuttingedge *** to traditional bioactivity-based screening methods,encoding and display technologies for combinatorial libraries have recently advanced from proof-of-principle experiments to promising tools for pharmaceutical hit discovery due to their high screening efficiency,throughput,and resource *** review systematically summarizes the development history,typology,and prospective applications of encoding and displayed technologies,including phage display,ribosomal display,mRNA display,yeast cell display,one-bead one-compound,DNA-encoded,peptide nucleic acidencoded,and new peptide-encoded technologies,and examples of preclinical and clinical *** discuss the progress of novel targeted therapeutic agents,covering a spectrum from small-molecule inhibitors and nonpeptidic macrocycles to linear,monocyclic,and bicyclic peptides,in addition to *** also address the pending challenges and future prospects of drug discovery,including the size of screening libraries,advantages and disadvantages of the technology,clinical translational potential,and market *** review is intended to establish a comprehensive high-throughput drug discovery strategy for scientific researchers and clinical drug developers.

关键词： Drug screening Phage display DNA-encoded chemical libraries Peptide-encoded chemical libraries Clinical drugs

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City level CO_(2) and local air pollutants co-control performance evaluation: A case study of 113 key envir on mental protection cities in China

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Advances in Climate Change Research 2022年第1期13卷 118-130页

作者： qing-yong ZHANG Bo-feng CAI Mu-Dan wang Jun-Xia wang You-Kai XING Guang-Xia DONG zhen ZHANG Xian-Qiang MAO School of Environment Beijing Normal University'Beijing 100875China Center for Climate Change and Environmental Policy Chinese Academy of Environmental PlanningBeijing 100012China China National Environmental Monitoring Centre Beijing 100012China Ministry of Transport Transport Planning and Research InstituteLaboratory of Transport Pollution Control and Monitoring TechnologyBeijing 100028China

‘Co-control',or synergistically reducing CO_(2)and local air polluta nt emissions,is an important strategy for cities to achieve'low carb on'and'blue sky'***,there were few studies to evaluate and compare the level of co-control of CO_(2) and local air pollutants in cities *** present study proposed qualitative and quantitative methods to evaluate the level of co-control of CO_(2)and three local air pollutant(SO_(2).NOX,and particulate matter PM)emissions in key environmental protection cities in China over two periods(2012-2015 and 2015-2018).Statistical analysis found that,though three local air pollutant emissions positively correlated with CO_(2) emission,no significantly positive correlation was found between local air pollutants emission reductions and CO_(2) emission reductions,indicating that co-control effects were poor in *** using the co-control effect coordinate system,qualitative evaluation showed that less than half of the sample cities could achieve co-control of the total amount of CO_(2) and local air *** employing the indicator of emission reduction equivalence(EReq),quantitative evaluation showed that the co-control level of the sample cities improved in 2015-2018 compared with *** regression analysis showed that,reducing coal consumption and economic development significantly enhanced the co-control performance of the observed *** present case study proved that the proposed methods for evaluation and comparison of the city co-control performance works well,and can be used in other countries and regions to promote global cities racing to carbon and local air pollutants co-control.

关键词： Co-control CO_(2)emission reduction Local air pollutant emission reduction:Performance evaluation Key environmental protection cities

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