检索结果-南通市图书馆

Mutational escape prevention by combination of four neutralizing antibodies that target RBD conserved regions and stem helix

引用

Virologica Sinica 2022年第6期37卷 860-873页

作者： Haoneng Tang yong ke Yunji Liao Yanlin Bian Yunsheng Yuan Ziqi Wang Li Yang Hang Ma Tao Sun Baohong Zhang Xiaoju Zhang Mingyuan Wu Jianwei Zhu Engineering Research Center of Cell and Therapeutic Antibody Ministry of EducationShanghai200240China School of Pharmacy Shanghai Jiao Tong UniversityShanghai200240China Department of Respiratory and Critical Care Medicine Zhengzhou University People's Hospital/Henan Provincial People's HospitalZhengzhou450003China School of Agriculture and Biology Shanghai Jiao Tong UniversityShanghai200240China Shanghai Municipal Veterinary key Laboratory Shanghai200240China Jecho Institute Shanghai200240China

New variants of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) appear rapidly every few *** have showed powerful adaptive ability to circumvent the immune system. To further understand SARS-CoV-2’s adaptability so as to seek for strategies to mitigate the emergence of new variants, herein we investigated the viral adaptation in the presence of broadly neutralizing antibodies and their combinations. First, we selected four broadly neutralizing antibodies, including pan-sarbecovirus and pan-betacoronavirus neutralizing antibodies that recognize distinct conserved regions on receptor-binding domain(RBD) or conserved stem-helix region on S2 *** binding competition analysis, we demonstrated that they were capable of simultaneously ***, a replication-competent vesicular stomatitis virus pseudotyped with SARS-CoV-2 spike protein was employed to study the viral adaptation. Twenty consecutive passages of the virus under the selective pressure of individual antibodies or their combinations were performed. It was found that it was not hard for the virus to adapt to broadly neutralizing antibodies, even for pan-sarbecovirus and pan-betacoronavirus antibodies. The virus was more and more difficult to escape the combinations of two/three/four antibodies. In addition, mutations in the viral population revealed by high-throughput sequencing showed that under the selective pressure of three/four combinational antibodies, viral mutations were not prone to present in the highly conserved region across betacoronaviruses(stem-helix region), while this was not true under the selective pressure of single/two ***, combining neutralizing antibodies targeting RBD conserved regions and stem helix synergistically prevented the emergence of escape mutations. These studies will guide future vaccine and therapeutic development efforts and provide a rationale for the design of RBD-stem helix tandem vaccine, which may help to impede

关键词： SARS-CoV-2 Adaptation Spike protein Stem helix Broadly neutralizing antibody

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

彝良县烤烟种植冰雹灾害风险区划及防御对策

引用

现代农业研究 2024年第5期30卷 116-120页

作者：周信荔林月王云江姜世荣陈剑辉张应东柯勇云南省彝良县气象局云南昭通657600 云南省昭通市气象局云南昭通657600

文章利用彝良县2013—2022年6~9月烤烟种植冰雹灾害、受损情况等气象资料,根据自然灾害系统理论和冰雹灾害风险评估的相关理论,根据彝良县烤烟种植冰雹灾害致灾因子危险性、承灾体易损性、孕灾环境敏感性、防灾减灾能力4个方面构建冰雹... 详细信息

文章利用彝良县2013—2022年6~9月烤烟种植冰雹灾害、受损情况等气象资料,根据自然灾害系统理论和冰雹灾害风险评估的相关理论,根据彝良县烤烟种植冰雹灾害致灾因子危险性、承灾体易损性、孕灾环境敏感性、防灾减灾能力4个方面构建冰雹灾害风险评估模型,结合GIS技术,对彝良县冰雹灾害进行精细化的风险区划,采用层次分析法和加权综合评价法计算各指标权重和风险指数,并利用GIS技术进行栅格图层计算得到彝良县冰雹灾害综合风险区划图。结果显示:彝良县冰雹灾害风险与地理环境有紧密的联系,二半山以上的乡镇海拔较高,冰雹灾害风险也高,而低矮河谷和平坝区出现冰雹灾害风险相对较低。冰雹灾害风险较高的地方集中在奎香乡、龙街乡和荞山镇东部。龙安镇、洛泽河镇地形相对平缓,高程差小,冰雹灾害发生频率偏低,属低风险区,如果合理布设防雹作业点并开展人工防雹作业,加强烤烟防雹减灾,是比较适合大规模、集中种植烟叶的。

关键词：烤烟冰雹 GIS 层次分析法风险区划

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of heat treatment on microstructures and properties of a heterostructured alloy with dissimilar components fabricated by WAAM

引用

Rare Metals 2024年第9期43卷 4441-4461页

作者： Ming-Cai Pan Jun-Qiang Xu yong Peng Ning-Ning Liang Si Lan Qi Zhou ke-Hong Wang School of Materials Science and Engineering Nanjing University of Science and TechnologyNanjing 210094China key Laboratory of Controlled Arc Intelligent Additive Manufacturing Technology Nanjing 210094China

Heat treatment significantly influences homogeneous material microstructures and mechanical properties,which can be improved by an optimal heat treatment ***,heat treatment application to heterogeneous materials presents significant challenges due to compositional and microstructural ***,a laminated heterostructured alloy comprising alternating316L stainless steel(SS)and 18Ni300 maraging steel(MS)layers fabricated using wire and arc additive manufacturing was investigated.A solution treatment was applied at900℃for 0.5 ***,the solution-treated and asfabricated(AF)samples were aged at 500℃for 4 h;these samples were denoted SA and AT,*** AT phase compositions and orientations were similar to those of *** SA 316L SS layer resembled that of AF,but the SA 18Ni300 MS layers exhibited a reduced austenite phase fraction and refined grain size,attributable to solid-state *** the AT sample,aging induced the formation of nanoscale acicularωphase and ellipsoidal Ni_(3)Ti,Fe_(2)Mo,and X precipitates in the 18Ni300 MS ***,the SA precipitates contained acicular Fe Ni_(2)and ellipsoidalω,Ni_(3)Ti,and X precipitates,and their fractions were lower than those in AT ***18Ni300 MS layer microhardness in the heat-treated samples increased due to nanoprecipitation,but the 316L SS layer microhardness resembled that of *** AT and SA ultimate tensile strengths increased to(1360±50)and(1473±41)MPa,respectively,attributable to precipitation *** SA 316L SS layer exhibited a high stress-induced martensite fraction,enhancing the ductility of heated samples.

关键词： Laminated heterostructured alloy Heat treatment Wire and arc additive manufacturing Microstructure Mechanical property

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Geochemical identification of a source rock affected by migrated hydrocarbons and its geological significance:Fengcheng Formation,southern Mahu Sag,Junggar Basin,NW China

引用

Petroleum Science 2024年第1期21卷 100-114页

作者： Wen-Long Dang Gang Gao Xin-Cai You ke-Ting Fan Jun Wu De-Wen Lei Wen-Jun He yong Tang State key Laboratory of Petroleum Resource and Prospecting China University of Petroleum(Beijing)Beijing102249China School of GeoSciences China University of Petroleum(Beijing)Beijing102249China Exploration&Development Institute Xinjiang Oil Field CompanyKaramay834000XinjiangChina

The Fengcheng Formation is a crucial source rock and the primary reservoir for oil accumulation in the Mahu *** oils are distributed throughout the Fengcheng Formation,ranging from the edge to the interior of the sag in the southern Mahu *** crude oils originate from in-situ source rocks in shallowly buried areas and the inner deep *** migration,the crude oil from the inner deep sag affects the source rocks close to carrier beds,leading to changes in the organic geochemical characteristics of the source *** changes might alter source rock evaluations and oil-source *** on data such as total organic carbon(TOC),Rock-Eval pyrolysis of source rocks,and gas chromatography-mass spectrometry(GC-MS)of the saturated fraction,and considering the geological characteristics of the study area,we define the identification characteristics of source rock affected by migrated hydrocarbons and establish the various patterns of influence that migrated hydrocarbons have on the source rock of the Fengcheng Formation in the southern Mahu *** source rocks of the Fengcheng Formation are mostly fair to good,containing mainly Type II organic matter and being thermally mature enough to generate *** rocks affected by migrated hydrocarbons exhibit relatively high hydrocarbon contents(S1/TOC>110 mg HC/g TOC,Extract/TOC>30%,HC:hydrocarbon),relatively low Rock-Eval Tmax values,and relatively high tricyclic terpane contents with a descending and mountain-shaped ***,biomarker composition parameters indicate a higher thermal maturity than in-situ source *** a comparison of the extract biomarker fingerprints of adjacent reservoirs and mudstones in different boreholes,three types of influence patterns of migrated hydrocarbons are identified:the edge-influence of thin sandstone-thick mudstone,the mixed-influence of sandstone-mudstone interbedded,and the full-influence of thick sandstone-thin *** finding reminds

关键词： Organic geochemistry Source rock Influence of migrated hydrocarbons Fengcheng Formation Southern Mahu Sag

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

FPGA-based position reconstruction method for neutron beam flux spatial distribution measurement in BNCT

引用

Nuclear Science and Techniques 2024年第3期35卷 96-108页

作者： Wei Jiang Ping Cao Yi-Ming Wu Xian-ke Liu Zhu-Jun Fang Zhi-yong Zhang Bin Shi Jun Chen State key Laboratory of Particle Detection and Electronics University of Science and Technology of ChinaHefei 230026China Department of Modern Physics University of Science and Technology of ChinaHefei 230026China School of Nuclear Science and Technology University of Science and Technology of ChinaHefei 230026China National key Laboratory for Metrology and Calibration Techniques China Institute of Atomic EnergyBeijing 102413China

A new measurement method for the spatial distribution of neutron beam flux in boron neutron capture therapy(BNCT)is being developed based on the two-dimensional Micromegas *** address the issue of long processing times in traditional offline position reconstruction methods,this paper proposes a field programmable gate array based online position reconstruction method utilizing the micro-time projection chamber *** method encapsulates key technical aspects:a self-adaptive serial link technique built upon the dynamical adjustment of the delay chain length,fast sorting,a coordinate-matching technique based on the mapping between signal timestamps and random access memory(RAM)addresses,and a precise start point-merging technique utilizing a circular combined *** performance test of the selfadaptive serial link shows that the bit error rate of the link is better than 10-12 at a confidence level of 99%,ensuring reliable data *** experiment utilizing the readout electronics and Micromegas detector shows a spatial resolution of approximately 1.4 mm,surpassing the current method’s resolution level of 5 *** beam experiment confirms that the readout electronics system can obtain the flux spatial distribution of neutron beams online,thus validating the feasibility of the position reconstruction *** online position reconstruction method avoids traditional methods,such as bubble sorting and traversal searching,simplifies the design of the logic firmware,and reduces the time complexity from O(n2)to O(n).This study contributes to the advancement in measuring neutron beam flux for BNCT.

关键词： Position reconstruction FPGA Readout electronics Neutron flux spatial distribution

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

混沌时间序列的混合粒子群优化预测

引用

控制与决策 2007年第5期22卷 562-565页

作者：刘伟王科俊邵克勇哈尔滨工程大学自动化学院哈尔滨150001 大庆石油学院电气工程系黑龙江大庆163318

提出一种混合粒子群优化算法,即在改进粒子群优化算法全局搜索模型参数的基础上,利用梯度下降法进一步确定径向基神经网络模型参数,以提高网络的收敛精度和网络性能.采用基于RBFNN的混合粒子群优化算法进行离散Henon和连续Mackey-Glass... 详细信息

提出一种混合粒子群优化算法,即在改进粒子群优化算法全局搜索模型参数的基础上,利用梯度下降法进一步确定径向基神经网络模型参数,以提高网络的收敛精度和网络性能.采用基于RBFNN的混合粒子群优化算法进行离散Henon和连续Mackey-Glass混沌时间序列预测仿真,结果表明该算法能快速精确地预测混沌时间序列,是研究复杂非线性动力系统辨识和控制的一种有效方法.

关键词：混沌时间序列粒子群算法径向基神经网络梯度下降法

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Proppant transport in rough fracture networks using supercritical CO_(2)

引用

Petroleum Science 2024年第3期21卷 1852-1864页

作者： yong Zheng Meng-Meng Zhou Ergun Kuru Bin Wang Jun Ni Bing Yang ke Hu Hai Huang Hai-Zhu Wang School of Petroleum Engineering Xi'an Shiyou UniversityXian710065ShaanxiChina Shaanxi key Laboratory of Carbon Dioxide Sequestration and Enhanced Oil Recovery Xi'an710075ShaanxiChina State key Laboratory of Petroleum Resources and Engineering China University of Petroleum(Beijing)Beijing2249China School of Mining and Petroleum Engineering University of AlbertaEdmontonTG2W2AlbertaCanad Shaanxi Yanchang Petroleum(Group)Co. Ltd.Xi'an710075ShaanxiChina

Proppant transport within fractures is one of the most critical tasks in oil,gas and geothermal reservoir stimulation,as it largely determines the ultimate performance of the operating *** transport in rough fracture networks is still a relatively new area of research and the associated transport mechanisms are still *** this study,representative parameters of rough fracture surfaces formed by supercritical CO_(2) fracturing were used to generate a rough fracture network model based on a spectral synthesis *** fluid dynamics(CFD)coupled with the discrete element method(DEM)was used to study proppant transport in this rough fracture *** reveal the turning transport mechanism of proppants into branching fractures at the intersections of rough fracture networks,a comparison was made with the behavior within smooth fracture networks,and the effect of key pumping parameters on the proppant placement in a secondary fracture was *** results show that the transport behavior of proppant in rough fracture networks is very different from that of the one in the smooth fracture *** turning transport mechanisms of proppant into secondary fractures in rough fracture networks are gravity-driven sliding,high velocity fluid suspension,and fracture structure *** the same injection conditions,supercritical CO_(2)with high flow Reynolds number still has a weaker ability to transport proppant into secondary fractures than *** of the supercritical CO_(2)needs to be increased beyond a certain value to have a significant effect on proppant carrying,and under the temperature and pressure conditions of this paper,it needs to be increased more than 20 times(about 0.94 m Pa s).Increasing the injection velocity and decreasing the proppant concentration facilitates the entry of proppant into the branching fractures,which in turn results in a larger stimulated reservoir *** results help to understand the pro

关键词： Reservoir stimulation CCUS Rough fracture network Supercritical CO_(2) Proppanttransport

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Down Regulated Expression of the β1 Subunit of the Big-conductance Ca2+ Sensitive K~+ Channel in Sphincter of Oddi Cells from Rabbits Fed with a High Cholesterol Diet

引用

Acta Biochimica et Biophysica Sinica 2006年第12期38卷 893-899页

作者： Pang DU Guang-Bin CUI Ya-Rong WANG Xiao-yong ZHANG ke-Jun MA Jing-Guo WEI* Department of Radiology,Tangdu Hospital,Fourth Military Medical University,Xi’an 710038,China Department of Radiology Tangdu Hospital Fourth Military Medical UniversityXi'an 710038 China

Hypercholesterolemia,which is closely related to gallbladder bile stasis,can cause sphincter ofOddi dysfunction (SOD) by increasing the tension of sphincter of Oddi (SO).Intracellular calcium ionconcentration ([Ca]) could influence the tension of *** β1 subunit of the big-conductance Casensitive K~+ channel (BKcan enhance the sensitivity of the BKchannel to [Ca].Absence and decline ofthe BKchannel subunit β1 could lead to many ***,the relationship betweenhypercholesterolemia and the expression of β1 subunit is not well *** this study,we successfullyexpressed and purified the rabbit BKβ1 subunit protein and prepared its polyclonal *** specificityof the prepared antibody was determined by western blotting.A SOD rabbit model induced by a high cholesteroldiet was established and the expression of the β1 subunit of SO was determined by immunohistochemicalstaining and western *** with the controls,our results demonstrated that hypercholesterolemiacould decrease the expression of the β1 subunit in the SO cells from *** indicates that lowerexpression of BKchannel β1 subunit might induce SOD.

关键词： hypercholesterolemia sphincter of Oddi dysfunction BK channel β1 subunit protein expression

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

我国青少年男子网球运动员身体素质特征与评价标准

引用

武汉体育学院学报 2014年第3期48卷 82-86页

作者：柯勇武汉体育学院网球教研室湖北武汉430079

采用问卷调查法、专家访谈法调查研究了第六届城运会比赛网球项目成绩排名前五名的省市,包括湖北、河南、天津、安徽、北京等的一线优秀网球运动员和教练员,并运用因子分析法对我国青少年男子网球运动员的身体素质特征及评价指标进行深... 详细信息

采用问卷调查法、专家访谈法调查研究了第六届城运会比赛网球项目成绩排名前五名的省市,包括湖北、河南、天津、安徽、北京等的一线优秀网球运动员和教练员,并运用因子分析法对我国青少年男子网球运动员的身体素质特征及评价指标进行深入而系统的分析,构建了我国青少年男子网球运动员身体素质专项化模型和评价标准,旨在为促进我国网球运动后备人才的可持续发展提供可操作性的依据。

关键词：青少年男子网球身体素质评价标准

来源：

维普期刊数据库

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

我国青少年男子网球运动员身体形态特征与评价标准研究

引用

天津体育学院学报 2014年第1期29卷 71-74,80页

作者：柯勇武汉体育学院网球教研室湖北武汉430079

运用文献资料法、问卷调查法和专家访谈法等研究方法,对第6届城运会比赛网球项目中成绩排名前5的湖北、河南、天津、安徽和北京等省市的一线优秀网球运动员和教练员进行分析。以男子网球运动员的身体形态研究为视角,综合文献资料法和问... 详细信息

运用文献资料法、问卷调查法和专家访谈法等研究方法,对第6届城运会比赛网球项目中成绩排名前5的湖北、河南、天津、安徽和北京等省市的一线优秀网球运动员和教练员进行分析。以男子网球运动员的身体形态研究为视角,综合文献资料法和问卷调查法确定了评价青少年男子网球运动员身体形态的44项原始指标,然后通过对这些原始指标采用利克特五级评分法进行赋值评分最终归纳出了在反映运动员身体素质上具有显著性差异的11项身体形态指标,分别是:身高、上肢长、足长、大腿围/身高×100、肩宽、体重、体型指数、身体水份含量、肌肉量推测值、去脂体重等。运用因子分析法和秩和比评价方法(RSR)建立了评价青少年男子网球运动员身体形态的结构模型,并对模型中各指标进行加权评分,采用百分位数法建立了各个指标的评价标准以及评价青少年男子网球运动员身体形态的综合评价标准,旨在为促进我国网球运动后备人才的可持续发展提供可操作性的依据。

关键词：青少年男子网球运动员身体形态评价标准

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：