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Photonics Research 2024年第4期12卷 821-832页

作者： WEI SHI yingchuan he JIANLIN WANG LULU ZHOU JIANWEI CheN LIWEI ZHOU ZEYU XI ZheN WANG KE FANG YIMING LI Department of Biomedical Engineering Southern University of Science and TechnologyShenzhen 518055China

Single-molecule localization microscopy(SMLM)enables three-dimensional(3D)investigation of nanoscale structures in biological samples,offering unique insights into their ***,traditional 3D super-resolution microscopy using high numerical aperture(NA)objectives is limited by imaging depth of field(DOF),restricting their practical application to relatively thin biological ***,we developed a unified solution for thick sample super-resolution imaging using a deformable mirror(DM)which served for fast remote focusing,optimized point spread function(PSF)engineering,and accurate aberration *** effectively correcting the system aberrations introduced during remote focusing and sample aberrations at different imaging depths,we achieved high-accuracy,large DOF imaging(~8μm)of the whole-cell organelles[i.e.,nuclear pore complex(NPC),microtubules,and mitochondria]with a nearly uniform resolution of approximately 35 nm across the entire cellular volume.

关键词： enable mirror resolution

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Rapid bacteria identification using structured illumination microscopy and machine learning

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Journal of Innovative Optical health Sciences 2018年第1期11卷 149-158页

作者： yingchuan he Weize Xu Yao Zhi Rohit Tyagi Zhe Hu Gang Cao College of Engineering Huazhong Agricultural University Wuhan 430070 P.R.China College of Veterinary Medicine Huazhong Agricultural University Wuhan 430070 P.R.China State Key Laboratory of Agricultural Microbiology Huazhong Agricultural University Wuhan 430070 P.R.China Bio-Medical Center Huazhong Agricultural University Wuhan 430070 P.R.China Key Laboratory of Development of Veterinary Diagnostic Products Ministry of Agriculture College of Veterinary Medicine Huazhong Agricultural University Wuhan 430070P.R.China

Traditionally,optical microscopy is used to visualize the morphological features of pathogenic bacteria,of which the features are further used for the detection and ident ification of the ***,due to the resolution limitation of conventional optical microscopy as well as the lack of standard pattern library for bacteria identification,the ffectiveness of this optical microscopy-based method is ***,we reported a pilot study on a combined use of Structured Illumination Microscopy(SIM)with machine learning for rapid bacteria *** applying machine learning to the SIM image datasets from three model bacteria(including Escherichia coli,Mycobacterium smegmatis,and Pseudomonas aeruginosa),we obtained a classifcation accuracy of up to 98%.This study points out a promising possibility for rapid bacterial identification by morphological features.

关键词： Structured ilumination microscopy bacterial classification principal component analysis support vector machine random forest

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汕揭高速公路汕头段常规桥梁的抗震设计

公路工程 2013年第3期38卷 155-157页

作者：何颖川广东省公路勘察规划设计院股份有限公司广东广州510507

汕揭高速公路汕头段常规桥梁设计过程中,按《公路桥梁抗震设计细则》(JTG/T B02-01-2008)的计算方法进行抗震计算分析及设计,抗震分析结果表明抗震设计成为控制本项目设计的因素。

关键词：高速公路常规桥梁桥梁设计

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Does Obesity Affect Preoperative Thoracolumbar/Lumbar Curve Flexibility in Adolescent Idiopathic Scoliosis

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Does Obesity Affect Preoperative Thoracolumbar/Lumbar Curve ...

第三届中西医结合脊柱及相关疾病学术年会

作者： Li Ming,Zhao yingchuan,Wang Chuanfeng,he Shisheng Spine Surgery,the Affiliated Changhai Hospital of the Second Military Medical University,Shanghai,200433,China

*** orthopeadic diseases have lone been presupposed to be related with obesity,obesity has become a main concerned problem in Chinese adolescents in recent *** effect of obesity on main thoracolumbar/lumbar curve flex...

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Circulating tumor DNA and its role in detection, prognosis and therapeutics of hepatocellular carcinoma

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Chinese Journal of Cancer Research 2024年第2期36卷 195-214页

作者： Sana Rashid yingchuan Sun Umair Ali Khan Saddozai Sikandar Hayyat Muhammad Usman Munir Muhammad Usman Akbar Muhammad Babar Khawar Zhiguang Ren Xinying Ji Malik Ihsan Ullah Khan Institute of Molecular Biology and Biotechnology University of LahoreLahore 54590Pakistan Department of Internal Oncology(Section Ⅰ) Xuchang Municipal Central HospitalXuchang 461000China Institute of Translational Medicine Medical CollegeYangzhou UniversityYangzhou 225001China Australian Institute for Bioengineering&Nanotechnology the University of QueenslandBrisbane 4072Australia Gomal Center of Biochemistry and Biotechnology Gomal UniversityDera Ismail Khan 29111Pakistan Applied Molecular Biology and Biomedicine Lab Department of ZoologyUniversity of NarowalNarowal Punjab 51600Pakistan Kaifeng Municipal Key Laboratory for Infection and Biosafety Henan International Joint Laboratory of Nuclear Protein RegulationSchool of Basic Medical SciencesHenan UniversityKaifeng 475004China Faculty of Basic Medical Subjects Shu-Qing Medical College of ZhengzhouZhengzhou 450064China Department of Medicine Huaxian County People's HospitalHuaxian 456400China

hepatocellular carcinoma(HCC) is considered the fifth most prevalent cancer among all types of cancers and has the third most morbidity value. It has the most frequent duplication time and a high recurrence rate. Recently, the most unique technique used is liquid biopsies, which carry many markers;the most prominent is circulating tumor DNA(ctDNA). Varied methods are used to investigate ctDNA, including various forms of polymerase chain reaction(PCR) [emulsion PCR(ePCR), digital PCR(dPCR), and bead, emulsion, amplification, magnetic(BEAMing) PCR]. hence ctDNA is being recognized as a potential biomarker that permits early cancer detection,treatment monitoring, and predictive data on tumor burden are subjective to therapy or surgery. Numerous ctDNA biomarkers have been investigated based on their alterations such as 1) single nucleotide variations(either insertion or deletion of a nucleotide) markers including TP53, KRAS, and CCND1;2) copy number variations which include markers such as CDK6, EFGR, MYC and BRAF;3) DNA methylation(RASSF1A, SEPT9, KMT2C and CCNA2);4) homozygous mutation includes ctDNA markers as CDKN2A, AXIN1;and 5) gain or loss of function of the genes, particularly for HCC. Various researchers have conducted many studies and gotten fruitful ***, there are some drawbacks to ctDNA namely low quantity, fragment heterogeneity, less stability, limited mutant copies and standards, and differential sensitivity. However, plenty of investigations demonstrate ctDNA's significance as a polyvalent biomarker for cancer and can be viewed as a future diagnostic, prognostic and therapeutic agent. This article overviews many conditions in genetic changes linked to the onset and development of HCC, such as dysregulated signaling pathways, somatic mutations, single-nucleotide polymorphisms, and genomic instability. Additionally, efforts are also made to develop treatments for HCC that are molecularly targeted and to unravel some of the genetic pathways that f

关键词： hepatocellular carcinoma circulating tumor DNA biomarkers single nucleotide variations diagnosis prognosis

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Comparison of factors affecting curve flexibility in adolescent and adult idiopathic scoliosis.

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Comparison of factors affecting curve flexibility in adolesc...

第三届中西医结合脊柱及相关疾病学术年会

作者： Li Ming,Chen Ziqiang,Zhao yingchuan,he Shisheng,Wang Chuanfeng Spine Surgery,the Affiliated Changhai Hospital of the Second Military Medical University,Shanghai,200433,China

Study Design.A retrospective analysis of radiographs in adolescent and adult idiopathic scoliosis patients. *** determine and compare factors affecting curve flexibility in adolescent

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The study of predictors of structural curves in Adolescent Idiopathic Scoliosis's minor curves

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The study of predictors of structural curves in Adolescent I...

第三届中西医结合脊柱及相关疾病学术年会

作者： Chen Ziqiang,Li Ming,Zhao yingchuan,he Shisheng,Wang Chuanfeng Spine Surgery,the Affiliated Changhai Hospital of the Second Military Medical University,Shanghai,200433,China

Study Design.A retrospective analysis of radiographs in adolescent scoliosis patients. *** determine the factors affecting a minor curve to be a structural one in adolescent idiopathic scoliosis patients.

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Correlation of Trunk Balance Immediately after Posterior Spinal Fusion with Postoperative Subjacent Disc Wedging in Adolescent Idiopathic Scoliosis

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Correlation of Trunk Balance Immediately after Posterior Spi...

第三届中西医结合脊柱及相关疾病学术年会

作者： Li Ming,Zhao yingchuan,he Shisheng,Zhu Xiaodong,Wang Chuanfeng Spine Surgery,the Affiliated Changhai Hospital of the Second Military Medical University,Shanghai,200433,China

Study Design.A retrospective radiographic study of surgically treated adolescent idiopathic scoliosis. *** investigate which radiographic parameter immediately after posterior

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Contrast in Secondary Organic Aerosols between the Present Day and the Preindustrial Period:The Importance of Nontraditional Sources and the Changed Atmospheric Oxidation Capability

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Advances in Atmospheric Sciences 2024年第10期41卷 1936-1952+2092-2100页

作者： yingchuan YANG Wenyi YANG Xueshun CheN Jiawen ZHU Huansheng CheN Yuanlin WANG Wending WANG Lianfang WEI Ying WEI Qian YE Huiyun DU Zichen WU Zhe WANG Jie LI Xiaodong ZENG Zifa WANG State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry Institute of Atmospheric Physics Chinese Academy of Sciences University of Chinese Academy of Sciences Chinese Academy of Environmental Planning International Center for Climate and Environment Sciences Institute of Atmospheric PhysicsChinese Academy of Sciences Institute of Urban Meteorology China Meteorology Administration Unit 93213 of PLA

Quantifying differences in secondary organic aerosols(SOAs) between the preindustrial period and the present day is crucial to assess climate forcing and environmental effects resulting from anthropogenic activities. The lack of vegetation information for the preindustrial period and the uncertainties in describing SOA formation are two leading factors preventing simulation of SOA. This study calculated the online emissions of biogenic volatile organic compounds(VOCs)in the Aerosol and Atmospheric Chemistry Model of the Institute of Atmospheric Physics(IAP-AACM) by coupling the Model of Emissions of Gases and Aerosols from Nature(MEGAN), where the input vegetation parameters were simulated by the IAP Dynamic Global Vegetation Model(IAP-DGVM). The volatility basis set(VBS) approach was adopted to simulate SOA formation from the nontraditional pathways, i.e., the oxidation of intermediate VOCs and aging of primary organic aerosol. Although biogenic SOAs(BSOAs) were dominant in SOAs globally in the preindustrial period, the contribution of nontraditional anthropogenic SOAs(ASOAs) to the total SOAs was up to 35.7%. In the present day, the contribution of ASOAs was 2.8 times larger than that in the preindustrial period. The contribution of nontraditional sources of SOAs to SOA was as high as 53.1%. The influence of increased anthropogenic emissions in the present day on BSOA concentrations was greater than that of increased biogenic emission changes. The response of BSOA concentrations to anthropogenic emission changes in the present day was more sensitive than that in the preindustrial period. The nontraditional sources and the atmospheric oxidation capability greatly affect the global SOA change.

关键词： anthropogenic and biogenic emissions IAP-AACM MEGAN secondary organic aerosol VBS

Effectiveness and safety of Shenfu injection in septic patients with hypoperfusion:A multi-center,open-label,randomized,controlled trial

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Journal of Intensive Medicine 2024年第4期4卷 484-490页

作者： Di Liu Tingting Pan Xiang Li Duming Zhu yingchuan Li Hongyu he Fang Wu Lijing Jiang Yang Chen Xiaoli Wang Jialin Liu Ruoming Tan Hongping Qu Department of Critical Care Medicine Ruijin HospitalShanghai Jiaotong University School of MedicineShanghaiChina Department of Critical Care Medicine Minhang HospitalFudan UniversityShanghaiChina Department of Critical Care Medicine Zhongshan HospitalFudan UniversityShanghaiChina Department of Critical Care Medicine Shanghai Jiaotong University Affiliated Sixth People’s HospitalShanghaiChina Department of Critical Care Medicine Tenth People’s Hospital of Tongji UniversityShanghaiChina

Background:To evaluate the effectiveness and safety of the Shenfu injection(SFI)combined with standard bundle treatment in septic patients with ***:This study was a multi-center,randomized,open-label,controlled trial conducted in four teaching hos-pitals in *** septic patients with hypoperfusion and traditional Chinese medicine(TCM)syndrome with Yang-Qi deficiency were enrolled from January 2019,through September *** patients were randomly allocated in a 1:1 ratio to either receive 60 mL of SFI infusion per day plus standard treatment(SFI group)or stan-dard bundle treatment alone(control group).The primary outcome was 28-day all-cause *** outcomes were 90-day all-cause mortality time to weaning from mechanical ventilation,time to weaning from vasopressors,time to discharge from the ICU and hospital,and laboratory results after ***:A total of 188 patients completed the *** study revealed that the results of the SFI group and the control groups were not statistically significant in 28-day all-cause mortality(10.6%vs.20.2%,respectively;P=0.106).The infusion of SFI was associated with a significant reduction in the duration of vasopressor use(median=4.0 days,interquartile range[IQR]:2.0 days-6.0 days ***=5.0 days,IQR:3.0 days-8.0 days,respectively;P=0.043).Patients in the SFI group had statistically greater reductions in plasma lactate levels compared with those in the control group at the first 12 h(median=1.1 mmol/L,IQR:0.3-2.0 mmol/L ***=0.0 mmol/L,IQR:−0.2 to 0.8 mmol/L,respectively;Pherapy did not significantly decrease 28-day all-cause mortality for septic patients with hypoperfusion and TCM syndrome with Yang-Qi deficiency.

关键词： Sepsis Shenfu injection Traditional Chinese medicine Prognosis