检索结果-南通市图书馆

低位直肠癌经括约肌间切除术后吻合口瘘对患者近远期肛门功能影响的临床分析

在线全文

学校读者我要写书评

暂无评论

中国普通外科杂志 2024年第4期33卷 561-568页

作者：张俐娜赵勇姜红红曹煜赵玉涓卓光鑽张斌尹淑慧王美丁健华中国人民解放军火箭军特色医学中心肛肠外科北京100088

背景与目的:经括约肌间切除术(ISR)作为超低位直肠癌的极限保肛手术,已被证实可获得良好的肿瘤学根治效果。因其能最大程度地保留患者肛门,避免永久性肠造口,已逐渐成为了低位直肠癌保肛手术的重要选择之一。然而,ISR手术由于切除了部... 详细信息

背景与目的:经括约肌间切除术(ISR)作为超低位直肠癌的极限保肛手术,已被证实可获得良好的肿瘤学根治效果。因其能最大程度地保留患者肛门,避免永久性肠造口,已逐渐成为了低位直肠癌保肛手术的重要选择之一。然而,ISR手术由于切除了部分或全部内括约肌,不可避免地会导致术后肛门控便功能的下降。吻合口瘘是ISR手术后最常见的并发症之一,可能对患者术后肛门功能产生不良影响。目前关于吻合口瘘对ISR术后患者的肛门功能的具体影响尚未有深入的研究。因此,本研究探讨吻合口瘘对ISR术后患者短期及长期肛门功能的影响。方法:回顾性收集中国人民解放军火箭军特色医学中心肛肠外科于2011年6月—2022年1月行ISR及预防性末端回肠造口术的236例患者临床及随访资料,比较发生与未发生吻合口瘘患者的一般临床资料、回肠造口还纳后6个月和2年Wexner失禁评分(WIS)与低位前切除综合征(LARS)评分以及肛门直肠测压各参数的差异。结果:236例患者中有41例(17.4%)发生吻合口瘘,23例(9.7%)最终未还纳回肠造口。与未发生吻合口瘘患者的一般临床资料比较结果显示,发生吻合口瘘患者BMI较高(27 kg/m^(2)vs.25 kg/m^(2))、合并糖尿病者较多(29.3%vs.15.9%)、手术时间较长(222 min vs.200 min),且造口未还纳比例明显升高(34.1%vs.4.6%),差异均有统计学意义(均P0.05)。在回肠造口还纳后6个月和2年均完成直肠肛管测压检查的共有53例患者,其中11例(20.8%)有吻合口瘘,42例(79.2%)无吻合口瘘。还纳后6个月,吻合口瘘患者的肛管最大收缩压高于无吻合口瘘患者(P0.05);还纳后2年,两组的肛门直肠测压各参数差异均无统计学意义(均P>0.05)。结论:吻合口瘘的发生增加了ISR术后永久性造口的风险。对恢复肠道连续性的患者,吻合口瘘对早期肛门功能有负面影响,但对远期肛门功能可能无明显影响。

关键词：直肠肿瘤经括约肌间切除术吻合口瘘肛管

Camouflaging attenuated Salmonella by cryo-shocked macrophages for tumor-targeted therapy

在线全文

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2024年第2期9卷 709-723页

作者： Leyang Wu Zengzheng Du Lin Li Liyuan Qiao shuhui Zhang Xingpeng yin Xiaoyao Chang Chenyang Li Zichun Hua The State Key Laboratory of Pharmaceutical Biotechnology and Department of Neurology of Nanjing Drum Tower Hospital School of Life Sciences and The Affiliated Hospital of Nanjing University Medical SchoolNanjing UniversityNanjing 21008 JiangsuChina Nanjing Generecom Biotechnology Co. LtdNanjing 210023China Changzhou High-Tech Research Institute of Nanjing University and Jiangsu TargetPharma Laboratories Inc Changzhou 213164 JiangsuChina School of Biopharmacy China Pharmaceutical UniversityNanjing 210023 JiangsuChina

Live bacteria-mediated antitumor therapies mark a pivotal point in cancer ***,the difficulty in reconciling the safety and efficacy of bacterial therapies has limited their *** bacterial tumor-targeted delivery while maintaining biosafety is a critical hurdle for the clinical translation of live microbial therapy for ***,we developed“dead”yet“functional”Salmonella-loaded macrophages using liquid nitrogen cold shock of an attenuated Salmonella typhimurium VNP20009-contained macrophage cell *** obtained“dead”macrophages achieve an average loading of approximately 257 live bacteria per 100 *** engineered cells maintain an intact cellular structure but lose their original pathogenicity,while intracellular bacteria retain their original biological activity and are delay freed,followed by ***“Trojan horse”-like bacterial camouflage strategy avoids bacterial immunogenicity-induced neutrophil recruitment and activation in peripheral blood,reduces the clearance of bacteria by neutrophils and enhances bacterial tumor enrichment efficiently after systemic ***,this strategy also strongly activated the tumor microenvironment,including increasing antitumor effector cells(including M1-like macrophages and CD8+Teffs)and decreasing protumor effector cells(including M2-like macrophages and CD4+Tregs),and ultimately improved antitumor efficacy in a subcutaneous H22 tumor-bearing mouse *** cryo-shocked macrophage-mediated bacterial delivery strategy holds promise for expanding the therapeutic applications of living bacteria for cancer.

关键词： shock holds maintain

Isotope effect of the stereodynamics in the reactions F+HCl→HF+Cl and F+DCl→DF+Cl

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2010年第34期55卷 3868-3874页

作者： yin shuhui GUO MingXing LI Lei LI XiangPing ZHANG yingHui Department of Physics Dalian Maritime University Dalian 116026 China Environmental Science and Engineering College Dalian Maritime University Dalian 116026 China

Using a new ground-state ab initio potential energy surface reported by Deskevich et al.,the product polarizations in the reactions F+HCl→HF+Cl and F+DCl→DF+Cl were studied by employing the quasi-classical trajectory *** a collision energy of 10 kcal/mol,the four generalized polarization-dependent differential cross-sections(2π/σ)(dσ00/dωt),(2π/σ)(dσ20/dωt),(2π/σ)(dσ22+/dωt)and(2π/σ)(dσ21-/dωt)were calculated in the center-of-mass *** distribution of the angle between k and j',P(θr),the distribution of the dihedral angle denoting the k-k'-j' correlation,P(φr),and the angular distribution of the product rotational vectors in the form of polar plots P(θr,φr)were also *** evident influence of isotope substitution on the product polarization is *** effect may result from the different mass factors of the two reactions.

关键词：同位素效应 DCL 反应盐酸高频氯准经典轨迹法替代产品

Theoretical study on stereodynamics of H+NeH^+(v=0,j=0)→H_2^++Ne reaction

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2012年第36期57卷 4712-4717页

作者： yin shuhui ZOU JingHan GUO MingXing XU XueSong GAO Hong LI Lei CHE Li Department of Physics Dalian Maritime UniversityDalian 116026China Environmental Science and Engineering College Dalian Maritime UniversityDalian 116026China

Stereodynamics of reaction H + NeH+(v = 0,j = 0) → H2+ + Ne is investigated by quasi-classical trajectory method using a new potential energy surface constructed by Lv et *** distributions of P(r),P(r) and PDDCSs are calculated at four different collision *** rotational polarization of product H2+ presents different characters at different collision *** product rotational angular momentum vector j' is not only aligned,but also oriented along the direction perpendicular to the scattering *** the increase of collision energy,the rotation of product molecule has a preference of changing from the "in-plane" mechanism to the "out-of-plane" *** the title reaction is mainly dominated by the direct reaction mechanism,the indirect mechanism plays a role when the collision energies are low.

关键词：反应机制立体碰撞能量 NE 经典轨迹分布计算势能面角动量

A single-nucleus transcriptomic atlas of primate liver aging uncovers the pro-senescence role of SREBP2 in hepatocytes

在线全文

学校读者我要写书评

暂无评论

Protein & Cell 2024年第2期15卷 98-120页

作者： Shanshan Yang Chengyu Liu Mengmeng Jiang Xiaoqian Liu Lingling Geng Yiyuan Zhang shuhui Sun Kang Wang jian yin Shuai Ma Si Wang Juan Carlos Izpisua Belmonte Weiqi Zhang Jing Qu Guang-Hui Liu Advanced Innovation Center for Human Brain Protection and National Clinical Research Center for Geriatric Disorders Xuanwu Hospital Capital Medical UniversityBeijing 100053China State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Aging Translational Medicine Center International Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital Medical UniversityBeijing 100053China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijing 100101China Xuanwu Hospital Capital Medical University Beijing 100053China University of Chinese Academy of Sciences Beijing 100049China Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijing 100101China Beijing Institute for Stem Cell and Regenerative Medicine Beijing 100101China Aging Biomarker Consortium Beijing 100101China Altos Labs Inc.San DiegoCA 94022USA

Aging increases the risk ofliver diseases and systemic susceptibility to aging-related ***,cell type-specific changes and the underlying mechanism of liver aging in higher vertebrates remain incompletely ***,we constructed the first single-nucleus transcriptomic landscape of primate liver aging,in which we resolved cell type-specific gene expression fluctuation in hepatocytes across three liver zonations and detected aberrant cell-cell interactions between hepatocytes and niche *** in-depth dissection of this rich dataset,we identifed impaired lipid metabolism and upregulation of chronic inflammation-related genes prominently associated with declined liver functions during *** particular,hyperactivated sterol regulatory element-binding protein(SREBP)signaling was a hallmark of the aged liver,and consequently,forced activation of SREBP2 in human primary hepatocytes recapitulated in vivo aging phenotypes,manifesting as impaired detoxification and accelerated cellular *** study expands our knowledge of primate liver aging and informs the development of diagnostics and therapeutic interventions for liver aging and associated diseases.

关键词： single-nucleus RNA sequencing liver hepatocytes aging senescence SREBP2

Recent progress on fluorescent probes for viruses

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2024年第1期35卷 72-83页

作者： Siyang Shen Weilin Xu Jianxiang Lu shuhui Wang Yurou Huang Xiaoyan Zeng Weimin Xiao Jun yin Key Laboratory of Pesticide and Chemical Biology Ministry of EducationHubei International Scientific and Technological Cooperation Base of Pesticide and Green SynthesisInternational Joint Research Center for Intelligent Biosensing Technology and HealthCollege of ChemistryCentral China Normal UniversityWuhan 430079China Shenzhen Academy of Metrology and Quality Inspection Shenzhen 518109China

Viruses are ubiquitous in human life. Some viruses can be used as vectors of genetic engineering and specific pesticides. Other viruses trigger a variety of diseases in humans, animals and plants, resulting in high infection rates and mortality. Therefore, convenient, accurate and rapid detection of viruses is of great significance for the diagnosis and treatment of subsequent diseases. In contrast to traditional methods of detection, which rely on time-consuming and complex techniques such as polymerase chain reaction (PCR), fluorescent probes and imaging methods generate real-time results, with high specificity, and have been widely used in viral detection. In this review, the application of viral fluorescent probes in analyzing the molecular structure, detection and biological imaging is discussed. In particular, we categorized the probes based on their specificity for human and plant viruses, reviewing the latest findings and analyzing their limitations. The potential of fluorescent molecular probes in the treatment of viral disease and environmental analysis, and their possible combinations with protein and immune technology are discussed.

关键词： Fluorescent probe Detection Bioimaging Virus Health

在线全文

学校读者我要写书评

暂无评论

直肠肛门抑制反射研究进展

中华胃肠外科杂志 2015年第12期18卷 1284-1288页

作者：尹淑慧赵克解放军第二炮兵总医院结直肠肛门外科北京100088

100多年来人们对直肠肛门抑制反射的认识不断深入，从发现该反射在先天性巨结肠诊断中具有重要意义，到其发生发育、反射通路、神经调控、生理功能等各方面都有了较深入的探索。目前已经认识到它在许多其它疾病中也都有较为特异的表现... 详细信息

100多年来人们对直肠肛门抑制反射的认识不断深入，从发现该反射在先天性巨结肠诊断中具有重要意义，到其发生发育、反射通路、神经调控、生理功能等各方面都有了较深入的探索。目前已经认识到它在许多其它疾病中也都有较为特异的表现。直肠肛门抑制反射已成为直肠肛管压力检测中不可缺少的一项重要内容。本文就直肠肛门抑制反射的研究进展做一综述。

关键词：直肠肛门抑制反射直肠肛管测压研究进展

掺杂质子化石墨相氮化碳增强碳基太阳能电池中钙钛矿的结晶质量

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemical Physics 2022年第2期35卷 390-398,I0004页

作者：郭明星刘文超黄均妍刘佳琦尹淑慧冷静大连海事大学环境科学与工程学院大连116026 大连海事大学理学院大连116026 中国科学院大连化学物理研究所分子反应动力学国家重点实验室大连116023

钙钛矿层的品质极大影响钙钛矿太阳能电池性能.然而,在溶液法生成多晶钙钛矿膜过程中会不可避免地形成缺陷和陷阱位.通过在钙钛矿层中嵌入添加物改善钙钛矿晶化,用于减少和钝化缺陷是非常重要的.本文合成一种环境友好的二维纳米材料质... 详细信息

钙钛矿层的品质极大影响钙钛矿太阳能电池性能.然而,在溶液法生成多晶钙钛矿膜过程中会不可避免地形成缺陷和陷阱位.通过在钙钛矿层中嵌入添加物改善钙钛矿晶化,用于减少和钝化缺陷是非常重要的.本文合成一种环境友好的二维纳米材料质子化石墨相氮化碳(p-g-C_(3)N_(4)),并掺杂于碳基钙太阳能电池的钙钛矿层中.实验证明,在钙钛矿前驱体溶液中添加p-g-C_(3)N_(4)不仅能调解碘铅甲胺(MAPbI_(3))结品的成核和生长速率,获得大晶粒尺寸的平滑表面,还能减少钙钛矿层的本征缺陷.质子化过程在氮化碳表面引入活性基团-NH_(2)/-NH_(3),它们和钙钛矿晶体表面N-H键发生强化学作用,有效地钝化电子陷阱,提高钙钛矿结晶质量.结果表明,与不掺杂的对照电池(效率为4.48%)和掺杂石墨相氮化碳(g-C_(3)N_(4))电池(效率为5.93%)相比,掺杂质子化石墨相氮化碳(p-g-C_(3)N_(4))的电池获得了6.61%的较高效率.本工作展示了一种通过掺杂改性添加物改善钙钛矿膜的简单方法,为碳基钙钛矿太阳能电池的低成本制备提供了建议.

关键词：钙钛矿太阳能电池碳对电极 p-g-C_(3)N_(4) 结晶质量光电性能

细胞色素P4502C19快代谢基因型缺血性卒中患者氯吡格雷抵抗的影响因素分析

在线全文

学校读者我要写书评

暂无评论

中国脑血管病杂志 2022年第2期19卷 109-115页

作者：尹书会曹清禹张倩王默力首都医科大学宣武医院神经内科北京100053 北京市第一中西医结合医院神经内科 100026 内蒙古自治区乌兰察布市集宁区中心医院重症监护室 012000

目的探讨细胞色素P4502C19(CYP2C19)快代谢基因型缺血性卒中患者氯吡格雷抵抗的影响因素。方法回顾性连续纳入CYP2C19快代谢基因型(CYP2C19*1/*1)缺血性卒中住院患者204例,均于确诊后连续服用氯吡格雷75 mg/d,共7 d,第8天晨起空腹抽取... 详细信息

目的探讨细胞色素P4502C19(CYP2C19)快代谢基因型缺血性卒中患者氯吡格雷抵抗的影响因素。方法回顾性连续纳入CYP2C19快代谢基因型(CYP2C19*1/*1)缺血性卒中住院患者204例,均于确诊后连续服用氯吡格雷75 mg/d,共7 d,第8天晨起空腹抽取静脉血,2 h内完成血栓弹力图血小板图的检测。由腺苷二磷酸(ADP)诱导的血小板抑制率0.05)。(2)二元Logistic回归分析结果显示,女性(OR=2.115,95%CI:1.066~4.196,P=0.032)、高血压病史(OR=2.196,95%CI:1.138~4.237,P=0.019)、高白细胞计数水平(OR=1.253,95%CI:1.016~1.545,P=0.035)均为氯吡格雷抵抗的独立危险因素。结论女性、高血压病、高白细胞计数水平可增加CYP2C19快代谢基因型缺血性卒中患者发生氯吡格雷抵抗的风险。

关键词：细胞色素P450酶系统缺血性卒中氯吡格雷抵抗细胞色素P4502C19 快代谢基因型影响因素分析