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Small particles,big effects:How nanoparticles can enhance plant growth in favorable and harsh conditions

Journal of Integrative Plant Biology 2024年第7期66卷 1274-1294页

作者： Jie Wang Honghong Wu Yichao Wang Wuwei Ye Xiangpei Kong zujun yin Zhengzhou Research Base State Key Laboratory of Cotton Bio‐breeding and Integrated UtilizationSchool of Agricultural SciencesZhengzhou UniversityZhengzhou 450001China State Key Laboratory of Cotton Bio‐breeding and Integrated Utilization Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyang 455000China National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China School of Engineering Design and Built EnvironmentWestern Sydney UniversityPenrith NSW 2751Australia Western Agricultural Research Center Chinese Academy of Agricultural SciencesChangji 831100China The Key Laboratory of Plant Development and Environmental Adaptation Biology Ministry of EducationCollege of Life SciencesShandong UniversityQingdao 266237China

By 2050,the global population is projected to reach 9 billion,underscoring the imperative for innovative solutions to increase grain yield and enhance food *** has emerged as a powerful tool,providing unique solutions to this ***(NPs)can improve plant growth and nutrition under normal conditions through their high surface-to-volume ratio and unique physical and chemical ***,they can be used to monitor crop health status and augment plant resilience against abiotic stresses(such as salinity,drought,heavy metals,and extreme temperatures)that endanger global *** of NPs can enhance stress tolerance mechanisms in plants,minimizing potential yield losses and underscoring the potential of NPs to raise crop yield and *** review highlights the need for a comprehensive exploration of the environmental implications and safety of nanomaterials and provides valuable guidelines for researchers,policymakers,and agricultural *** thoughtful stewardship,nanotechnology holds immense promise in shaping environmentally sustainable agriculture amid escalating environmental challenges.

关键词： abiotic stress agricultural defense system nano-particles nanotechnology oxidative stress reactive oxygen species toxicity

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Characters and structures of the nucleobase-ascorbate transporters(NAT)family genes in Gossypium hirsutum and their roles in responding to salt and drought stresses

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Journal of Cotton Research 2022年第2期5卷 115-129页

作者： GUO Lixue ZHAO Lanjie LU Xuke CHEN Xiugui WANG Shuai WANG Junjuan WANG Delong yin zujun CHEN Chao FAN Yapeng ZHANG Yuexin YE Wuwei State Key Laboratory of C otton Biology/I nstitute of Cott on Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base School of Agricultural SciencesZhengzhou University/Key Laboratory for Cotton Genetic ImprovementMOARAAnyang 455000HenanChina

Background: Nucleobase-ascorbate transporters(NAT), synonymously called nucleobase-cation symporter 2(NCS2) proteins, were earlier reported to be involved in plant growth, development and resistance to stress. Previous studies concluded that s a polymorphic SNP associated with NAT12 was significant di erent between salt-tolerant and salt-sensitive materials of upland cotton. In current study, a comprehensive analysis of NAT family genes was conducted for the first time in ***: In this study, we discovered 32, 32, 18, and 16 NAT genes in Gossypium hirsutum, G. barbadense, G. raimondii and G. arboreum, respectively, which were classified into four groups(groups I–IV) based on the multiple sequence analysis. These GhNAT genes were unevenly distributed on At and Dt sub-genome in G. hirsutum. Most GhNAT members in the same group had similar gene structure characteristics and motif composition. The collinearity analysis revealed segmental duplication as well as tandem duplication contributing to the expansion of the GhNATs. The analysis of cis-acting regulatory elements of GhNATs showed that the function of GhNAT genes in cotton might be related to plant hormone and stress response. Under di erent conditions, the expression levels further suggested the GhNAT family genes were associated with plant response to various abiotic stresses. GhNAT12 was detected in the plasma membrane. And it was validated that the GhNAT12 gene played an important role in regulating cotton resistance to salt and drought stress through the virus-induced gene silencing(VIGS) ***: A comprehensive analysis of NAT gene family was performed in cotton, including phylogenetic analysis, chromosomal location, collinearity analysis, motifs, gene structure and so on. Our results will further broaden the insight into the evolution and potential functions of NAT genes in cotton. Current findings could make significant contribution towards screening more candidate genes related to b

关键词： Gossypium hirsutum NAT gene family VIGS Salt and drought stresses

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早熟棉花品种湘棉早3号

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中国棉花 2024年第8期51卷 47-48,51,F0002页

作者：王俊娟王帅彭凡嘉王德龙陆许可阴祖军陈修贵郭丽雪赵兰杰陈超叶武威中国农业科学院棉花研究所/棉花生物育种及产业技术国家工程研究中心/农业农村部棉花遗传改良重点开放实验室河南安阳455000 湖南省棉花科学研究所湖南常德415101

湘棉早3号于2022年4月通过湖南省农作物品种审定委员会审定,2023年5月获得植物新品种权授权。该品种为非转基因早熟常规棉品种,2020年湖南省早熟棉生产试验中平均每666.7 m^(2)皮棉产量为93.3 kg,比对照省工棉2号增产3.9%。概述了湘棉早... 详细信息

湘棉早3号于2022年4月通过湖南省农作物品种审定委员会审定,2023年5月获得植物新品种权授权。该品种为非转基因早熟常规棉品种,2020年湖南省早熟棉生产试验中平均每666.7 m^(2)皮棉产量为93.3 kg,比对照省工棉2号增产3.9%。概述了湘棉早3号的选育经过、特征特性、适宜种植区域,并总结了其关键栽培技术。

关键词：棉花湘棉早3号品种选育早熟适应性

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循环肿瘤细胞在肺癌转移防治中作用的研究进展

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中国肿瘤 2024年第8期33卷 661-667页

作者：陈培清赵宇侠阙祖俊上海健康医学院协同科研中心上海中医药大学附属市中医医院肿瘤研究所

转移是导致肺癌患者死亡的最主要原因，而目前临床上仍缺乏特异性有效的防治肺癌转移进而提高患者生存的治疗药物。循环肿瘤细胞（circulating tumor cells,CTCs）作为肿瘤转移的“种子”，其数目和表型的动态变化过程，可实时反映出患... 详细信息

转移是导致肺癌患者死亡的最主要原因，而目前临床上仍缺乏特异性有效的防治肺癌转移进而提高患者生存的治疗药物。循环肿瘤细胞（circulating tumor cells,CTCs）作为肿瘤转移的“种子”，其数目和表型的动态变化过程，可实时反映出患者体内转移的发生及进展。全文聚焦于CTCs在肺癌根治术后转移防治中的作用，探讨其作为转移风险评估和临床疗效评价的潜力。CTCs有望成为抗肿瘤转移治疗的新靶点，以期为肺癌转移防治提供思路。

关键词：循环肿瘤细胞肺癌转移生物标志物治疗靶点

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Identification and Characterization of Salt-responsive MicroRNAs and Their Target Genes in Cotton

Identification and Characterization of Salt-responsive Micro...

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2015年全国棉花青年学术研讨会

作者： yin zujun Wang Junjuan Wang Delong Fan Weili Wang Shuai Ye Wuwei State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese Academy of Agricultural Sciences

Soil salinity is one of the most serious factors limiting the productivity of agricultural *** salt concentrations in the soil affect various aspects of plant growth and development,such as inhibition of enzymatic activities and reductions in photosynthetic *** respond to stress using multiple gene regulatory mechanisms including post-transcriptional regulation of gene ***（miRNAs）,an abundant class of short non-coding（21-23 nt） RNAs,are generated from single-strand hairpin RNA precursors and are sometimes conserved over long evolutionary *** regulate gene expression by binding to targeted mRNA sequences in a perfect or near-perfect complementary site,leading to mRNA cleavage or,in a few cases,translational repression in *** this study,three cotton small RNA libraries were constructed and sequenced under normal conditions,short-term salt stress（SS） and long-term salt stress（LS）.The length distribution of obtained small RNAs was significantly different between *** SS sample showed significantly higher 21-nt small RNA abundance（48.86%） than CK（40.63%） and the LS sample（36.31%）.To investigate the repertoire of miRNAs,small RNA reads in three small RNA libraries were first mapped to protein-coding and structural RNA-coding regions in the Rfam and GenBank ***,tRNAs,snRNAs and snoRNAs were ***,the remaining sequences were aligned with all known miRNAs in the central miRNA Registry Database,miRBase *** a result,a total of 201 conserved miRNAs were *** were classified into 88 families on the basis of sequence *** remaining non-encoding sequences were used to predict novel miRNAs using the MIREAP program,based on their secondary *** 24 of them,the complementary miRNA species were cloned in libraries,providing an indication of precise excision from the stem-loop *** first made a comparative analysis of miRNA expression between CK and SS lib

关键词： cotton microRNAs target genes ARFs

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Identification and Expression Analysis Mitogen-Activated Protein Kinase Signalling Cascades in Cotton

Identification and Expression Analysis Mitogen-Activated Pro...

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中国农学会棉花分会40年征文暨2019年年会

作者： Zhu Weidong Wang Junjuan Wang Delong Wang Shuai Guo Lixue yin zujun Ye Wuwei State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese Academy of Agricultural Sciences

Mitogen-activated protein kinase(MAPK) cascades are involved in plant growth and development,as well as play a central role in response to biotic and abiotic ***,few complete MAPK signaling cascades have been characterized in *** this study,a total of 169 MAPKKK,25 MAPKK and 56 MAPK genes were identified in Gossypium hirsutum genome and classified into distinct groups supported by the phylogenetic *** structures and conserved motifs analyses pointed out that the majority of MAPKKK,MAPKK and MAPK genes in the same subfamily shared similar gene structures and conserved ***,analyses of cis-acting regulatory elements in promoter regions revealed that MAPK cascades might participate in plant responses to diverse abiotic stresses and ***,the different stages of transcriptome analysis demonstrated that 39 MAPKKK genes,10 MAPKK genes and 20 MAPK genes play an important role in drought stress *** the similarity of expression patterns could indicate the correlation and the similar biological function of *** addition,interaction networks analyses of three gene families suggest that MAPK signalling cascade mediate network participates in multiple stress *** are some high confidence interactive proteins involved in MAPKKK,MAPKK and MAPK networks,including MEKK1,WRKY33,MKKs,and ***,our study will provide a useful reference for further functional analysis of MAPK cascades in cotton.

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Comparative Analysis of Salinity-Induced Proteomic Changes in Cotton (Gossypium hirsutum L.)

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Agricultural Sciences 2015年第1期6卷 78-86页

作者： Yupeng Cui Xuke Lu Delong Wang Junjuan Wang zujun yin Weili Fan Shuai Wang Wuwei Ye State Key Laboratory of Cotton Biology Institute of Cotton Research of Chinese Academy of Agricultural Sciences Anyang China

Salt stress on cotton varieties of distinct salinity tolerance can induce expression of different proteins. Zhong 07, a salt-tolerant variety and Zhong s9612, a salt-sensitive variety, were utilized as experimental materials. The leaves of trefoil seedlings treated with or without 0.4% NaCl for 24 h were harvested for whole-protein extraction. Two-dimensional technology, combined with mass spectroscopy (MS) analysis and protein database searching, was employed to detect differentially expressed proteins and determine their identities and biological functions. Compared with the control, Zhong 07 showed 10 differentially expressed proteins under salt stress, of which 6 were upregulated and 4 were downregulated. Meanwhile, 12 differentially expressed proteins were detected in Zhong s9612 under salt stress, of which 10 were upregulated and 2 were downregulated. In the matrix-assisted laser desorption-ionization/time of flight-time of flight/MS analysis, 14 differentially expressed proteins were successfully identified, including the ribulose-1, 5-bisphosphate carboxylase/oxygenase (RuBisco) large subunit-binding protein subunit alpha (RuBisco α), luminal binding protein (LBP), heat shock protein 70 (Hsp1, 2, 3), pathogenesis-related protein class 10 (PR-10), quinoneoxidoreductase-like protein (QOR), S-adenosylmethioninesyn-thetase (SAMS), enolase (EN), and RuBisco large subunit-binding protein subunit beta (RuBisco β). Cellular function is ultimately executed by proteins, and cotton varieties with different salt tolerance can be influenced by salt stress to various degrees, which can provide certain theoretical foundation for the identification of salt tolerance of cotton varieties. The findings also provide some proteins, such as the RuBisco large subunit binding proteins α and β subunits, OEE2 protein, HSP70, and S-adenosylmethionine synthetase, which can be used as protein markers of salt-to-lerance before- and post-treatment, making a big difference in salt-toleranc

关键词： Upland Cotton (Gossypium hirsutum L.) Salt Stress Differential Protein Mass Spectrum Analysis

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Transcriptome-Wide Identification of Salt-Responsive miRNA Targets in Cotton Using Degradome Sequencing

Transcriptome-Wide Identification of Salt-Responsive miRNA T...

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中国农学会棉花分会2016年年会

作者： yin zujun Wang Junjuan Wang Delong Fan Weili Wang Shuai Guo Lixue Ye Wuwei State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese Academy of Agricultural Sciences

＜正＞MicroRNAs（miRNAs）are a class of endogenous,non-coding small RNAs that play important roles in many developmental processes and stress responses in *** finely modulate gene expression by binding to targeted mRN...

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不确定性缓冲下农业产业组织演化的逻辑与进路

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财经科学 2024年第7期 138-148页

作者：廖祖君侯宏凯四川省社会科学院“城乡融合发展改革试验研究”科研创新团队四川省哲学社会科学重点研究基地西部乡村振兴研究中心四川农业大学经济学院

培育、创新和完善农业产业组织体系是推进中国式农业现代化的重要支撑。引入不确定性缓冲视角,提出“不确定性冲击→缓冲策略选择→适应性选择与迭代”的农业产业组织演化分析框架,指出农业产业组织演化是在复杂不确定性下适应性选择与... 详细信息

培育、创新和完善农业产业组织体系是推进中国式农业现代化的重要支撑。引入不确定性缓冲视角,提出“不确定性冲击→缓冲策略选择→适应性选择与迭代”的农业产业组织演化分析框架,指出农业产业组织演化是在复杂不确定性下适应性选择与迭代的过程。实践表明,改革开放以来,为缓冲供需脱节、食品安全与动植物疫病、农产品消费升级与供应链安全等特定阶段的不确定性冲击,中国农业产业组织呈现出“小农经营→‘中介组织+农户’→准纵向一体化组织→农业产业化联合体”的演化路径,农业产业化联合体成为当前及未来中国农业产业组织演化的进路选择。应鼓励龙头企业引领建设农业产业化联合体,并通过一系列制度设计降低农业生产经营面临的不确定性风险。

关键词：不确定性缓冲 “中介组织+农户” 准纵向一体化组织农业产业化联合体

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Whole Genome Analysis of the CIPK Gene Family in the Diploid Cotton

Whole Genome Analysis of the CIPK Gene Family in the Diploid...

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中国棉花学会2015年年会

作者： Wang Junjuan Ye Wuwei Lu Xuke yin zujun Zhao Xiaojie Wang Delong Fan Weili Wang Shuai Yu Shuxun State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese Academy of Agricultural Sciences

As a second messenger,calcium participates in a large number of processes of abiotic stresses and plays an important role in the process of response to adversity signaling process in *** transmits signals to downstream through the combination with protein phosphatase B subunit like protein and calcineurin B-like protein-interacting protein kinase（CIPK）plays a key regulatory role in the growth,development and adversity of *** present,the research on the CIPK gene family mainly focused on the model plants such as Arabidopsis thaliana and rice,but the reports of the CIPK gene family in cotton were *** the present study,the identification of the CIPK gene family was carried out on the whole genomes of diploid cotton（Gossypium raimondii L.）and Asian cotton Shixiya I（Gossypium arboreum L.）,and bioinformatics analysis such as gene structure was conducted on the identified CIPK gene family members to study the phylogenetic relationship and analyze the transcript expression of the CIPK gene family in different tissues in different *** results demonstrated that the CIPK gene family was unevenly distributed whether in the cotton D genome chromosome or A genome chromosome.A total of 41 CIPK genes were identified in the D genome of the Gossypium raimondii,mainly distributed in chromosome 10and chromosome 9 but not chromosome 2 or chromosome 12;A total of 39 CIPK genes were identified in the A2 genome of the diploid Gossypium arboreum L.,mainly distributed in chromosome 8and chromosome 11 but not chromosome 2 or chromosome *** CIPK gene family in cotton can be classified into two types according to the structures of the genes,the intron rich type and intronless type（or rare intron type）,which are similar to those of the CIPK gene families of Arabidopsis thaliana,maize,rice and other *** analysis revealed that the CIPK gene family members in cotton had high evolutionary relationships with those of the dicotyledon Arabidopsis thaliana an

关键词： Gossypium raimondii Gossypium arboreum L. CIPK gene family transcriptional expression CBL-CIPK signaling network

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