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Nitric Oxide Content in Wheat Leaves and Its Relation to Programmed Cell Death of Main Stem and Tillers Under Different Nitrogen Levels

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Journal of Integrative Agriculture 2013年第2期12卷 239-250页

作者： GUO Jun-xiang CHEN Er-ying yin yan-ping WANG Ping LI Yong CHEN Xiao-guang WU Guang-lei WANG Zhen-lin National Key Laboratory of Crop Biology Agronomy College Shandong Agricultural University Xuzhou Sweetpotato Research Center/Sweetpotato Research Institute Chinese Academy of Agricultural Sciences

Nitric oxide （NO） is a key signaling molecule in different physiological processes of plants, including programmed cell death （PCD）. PCD of tillers plays an important role in surviving which are major components of grain yield. PCD was triggered in wheat leaves of main stem and tillers by NO content under different nitrogen treatments. In wheat, NO could be synthesized endogenously by nitrate reductase （NR）. As an inducible enzyme, NR activity was closely related to substrate concentration. Therefore, different nitrogen levels would change NR activity and NO production. The objective of this study was to determine the effects of NR activity, NO production, and the correlation between them on different tillers growth, development, senescence, and kernel protein content under different nitrogen levels. Field-experiments were conducted in 2009-2011 growing seasons, using two wheat cultivars with different spike-types. Results showed that for main stem and primary tillers, NR activity and NO content reached high level at heading stage, while for secondary tiller, the level of NR activity was low, but NO content was high in the present research. The NO synthesis depending on NR activity in wheat leaves was significant in the early growing stage, but the NO synthesis weakened with the progress of growing period. NO was related to the senescence of wheat leaves, but PCD was more sensitive to marked changes of NO content than NO content itself. N application had marked influence on the aging process of primary tiller, while had little influence on that of main stem and secondary tiller. Moreover, N fertilizer application could increase spike rate and protein content of primary tiller by N fertilizer application.

关键词： wheat nitric oxide programmed cell death tiller

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殷燕云心肾合治理论治疗早发性卵巢功能不全经验探析

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江西中医药 2021年第12期52卷 15-17页

作者：朱珠殷燕云南京中医药大学第一临床医学院南京210029

殷燕云根据国医大师夏桂成归纳总结的补肾调周法,结合女性“阳常有余,阴常不足”的生理特点,认为早发性卵巢功能不全根本病机在于肾阴不足,心火独亢,心肾失交。治疗上着重肾阴的恢复,以补肾滋阴、宁心降火为总治则,同时注重调畅患者情... 详细信息

殷燕云根据国医大师夏桂成归纳总结的补肾调周法,结合女性“阳常有余,阴常不足”的生理特点,认为早发性卵巢功能不全根本病机在于肾阴不足,心火独亢,心肾失交。治疗上着重肾阴的恢复,以补肾滋阴、宁心降火为总治则,同时注重调畅患者情志、改善睡眠,临床疗效显著。

关键词：早发性卵巢功能不全心肾失交滋肾宁心

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Simultaneous determination of flavonoids and anthraquinones in chrysanthemum by capillary electrophoresis with amperometry detection

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Chinese Chemical Letters 2010年第10期21卷 1231-1234页

作者： yin yan Zhang Zi Cheng Li Jin Kun Zhu Zhi Yong yang Qing Jiang Wang Pin Gang He Yu Zhi Fang Department of Chemistry East China Normal University Shanghai 200062 China

A high-performance capillary electrophoresis with amperometry detection method （CE-AD） has been developed for the analysis of flavonoids and anthraquinones （emodin, kaempferol, apigenin, luteolin and rhein） in chrysanthemum. Under optimum conditions, these five analytes were base-line separated within 17 min using a borate-phosphate running buffer （1.5 ×10^-2 mol/L borate-3× 10^-2 mol/L phosphate running buffer, pH 9.0） at a working potential of ＋0.90 V （vs. SCE） and a separation voltage of 19 kV. The linear relationship between concentration and current response was obtained with detection limits （S/N = 3） ranging from 1.0 × 10^-7 to 2.1 × 10^-7 g/mL for all analytes. This proposed method was successfully used in the analysis of four kinds of chrysanthemum with relatively simple extraction procedures, the assay results were satisfactory.

关键词： Capillary electrophoresis Amperometry detection Chrysanthemu Flavonoids Anthraquinones

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Effect of the feeding rate on microstructure and properties of plasma spheroidized GH4169 powder

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China Welding 2020年第1期29卷 37-44页

作者： yin yan Zhao Chao Lu Chao Xiao Mengzhi Zhang Ruihua State Key Laboratory of Advanced Processing and Recycling of Nonferrous Metals Lanzhou University of TechnologyLanzhou 730050China China Iron&Steel Research Institute Group Beijing 100081China Hard-ware Knife Cut Industrial Technology Research Institute yangjiang Yangjiang 529533China

The growing interest in additive manufacturing of GH4169 alloy was accompanied by the demand on spherical GH4169 powders with high *** powder particles were treated by radio frequency plasma with the different feeding *** microstructure and morphology,the particle size distribution of as-treated powders were studied by scanning electron microscopy and laser particle size *** was demonstrated that GH4169 powders with extremely fine followability were obtained by radio frequency plasma spheroidization *** the same plasma parameters,the spheroidization efficiency of the particles varied with the feeding *** the rate of the powder feeding rates was too small,the excessive absorption of the heating by the powders caused vaporization,then the collection *** the feeding rates was too large,the powder particles were insufficiently absorbed,resulting in defects in the *** microstructure of the as-treated spherical particles was mainly cell crystals,columnar crystals,and even *** the suitable plasma parameters,the resulting powders haved a slightly increased average particle size,excellent spheroidization,surface smoothness,followability,and bulk ratio.

关键词： GH4169 radio frequency plasma spheroidization the feeding rate

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Simultaneous determination of active ingredients in blueberry wine by CE-AD

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Chinese Chemical Letters 2011年第10期22卷 1237-1240页

作者： Zi Cheng Li Lang Zhu Chi Jin Kun Zhu yin yan Zhang Qing Jiang Wang Pin Gang He Yu Zhi Fang Department of Chemistry East China Normal University Shanghai 200062 China

A method based on capillary electrophoresis coupled with amperometric detection（CE-AD） has been developed for the separation and determination of kaempferol,ferulic acid,vanillic acid,caffeic acid,gallic acid and protocatechuic acid in blueberry wine for the first *** order to get the optimum conditions of separation,the effects of working electrode potential,pH value and concentration of running buffer,separation voltage and injection time on CE-AD were *** the optimum conditions, the analytes could be separated in Na_2B_4O_7-KH_2PO_4 buffer at pH 7.8 within 18 min.A 300μm diameter carbon disk electrode had good current responses at ＋0.95 V（***） for all *** responses were linear with concentrations over three orders of magnitude with detection limits（S/N = 3） at 10^（-8） g/mL magnitude for the analytes and the recoveries were in the range of 95.8- 106.7%.The method could be successfully applied to the analysis of real sample with satisfactory results and therefore recommended for use by the quality control departments of fruit wine producers.

关键词： Application Capillary electrophoresis Amperometric detection Blueberry wine

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In vitro Biocompatibility Assessment of a Novel PRGD/PDLLA/NGF Composite Material

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Journal of Wuhan University of Technology(Materials Science) 2011年第6期26卷 1059-1063页

作者：殷义霞李世普 Biomaterials and Engineering Research Center Wuhan University of Technology

The objective of this study was to evaluate the degradability and biocompatibility of a novel composite materials which was grafted with RGD and immobilized with NGF（PRGD/PDLLA/NGF）. The releasing of NGF, the biodegradability and cell-biocompatibility of PRGD/PDLLA/NGF membrane were evaluated in vitro. The experimental results showed that the NGF release process was prolonged over 30 days. Furthermore, the PRGD/PDLLA/NGF showed a better hydrophilicity, better biodegradation properties and cells affinity than PDLLA, which means a good support to adhesion and proliferate of Schwann cells. Therefore, the novel composite material holds considerable promise as scaffolds in nerve tissue engineering.

关键词： PRGD/PDLLA/NGF schwann cells biocompatibility scaffold

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Degradation prediction of proton exchange membrane fuel cell stack using semi-empirical and data-driven methods

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Energy and AI 2023年第1期11卷 1-11页

作者： Yupeng Wang Kangcheng Wu Honghui Zhao Jincheng Li Xia Sheng yan yin Qing Du Bingfeng Zu Linghai Han Kui Jiao State Key Laboratory of Engines Tianjin University135 Yaguan RoadTianjin300350China Powertrain Department General R&D InstituteFAWNo.1 Xinhongqi StreetChangchunJilin 130013China Internal Combustion Engine Research Institute Tianjin University92 Weijin RoadTianjin300072China

Degradation prediction of proton exchange membrane fuel cell(PEMFC)stack is of great significance for improving the rest useful *** this study,a PEMFC system including a stack of 300 cells and subsystems has been tested under semi-steady operations for about 931 ***,two different models are respectively established based on semi-empirical method and data-driven method to investigate the degradation of stack *** is found that the root mean square error(RMSE)of the semi-empirical model in predicting the stack voltage is around 1.0 V,while the predicted voltage has no local dynamic characteristics,which can only reflect the overall degradation trend of stack *** RMSE of short-term voltage degradation predicted by the DDM can be less than 1.0 V,and the predicted voltage has accurate local variation ***,for the long-term prediction,the error will accumulate with the iterations and the deviation of the predicted voltage begins to fluctuate gradually,and the RMSE for the long-term predictions can increase to 1.63 *** on the above characteristics of the two models,a hybrid prediction model is further *** prediction results of the semi-empirical model are used to modify the input of the data-driven model,which can effectively improve the oscillation of prediction results of the data-driven model during the long-term *** is found that the hybrid model has good error distribution(RSEM=0.8144 V,R2=0.8258)and local performance dynamic characteristics which can be used to predict the process of long-term stack performance degradation.

关键词： Proton exchange membrane fuel cell system Data-driven method Semi-empirical equation Degradation experiments

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Investigation of stimulated Raman scattering in longitudinal magnetized plasma by theory and kinetic simulation

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Plasma Science and Technology 2021年第11期23卷 26-34页

作者： Hongyu ZHOU yan yin Kaiqiang PAN Chengzhuo XIAO Jinlong JIAO Duan XIE Tongpu YU Fuqiu SHAO Hongbin ZHUO Department of Physics National University of Defense TechnologyChangsha 410073People's Republic of China Laser Fusion Research Center China Academy of Engineering PhysicsMianyang 621900People's Republic of China Key Laboratory for Micro-/Nano-Optoelectronic Devices of Ministry of Education&Hunan Province Key Laboratory of Low-Dimensional Structured Physics and Devices School of Physics and ElectronicsHunan UniversityChangsha 410082People's Republic of China Collaborative Innovation Center of IFSA(CICIFSA) Shanghai Jiao Tong UniversityShanghai 200240People’s Republic of China School of Physics Peking UniversityBeijing 100871People’s Republic of China School of Electronic Information and Electrical Engineering Changsha UniversityChangsha 410003People’s Republic of China Center for Advanced Material Diagnostic Technology Shenzhen Technology UniversityShenzhen 518118People’s Republic of China

Stimulated Raman scattering(SRS)in a longitudinal magnetized plasma is studied by theoretical analysis and kinetic *** linear growth rate derived via one-dimensional fluid theory shows the dependence on the plasma density,electron temperature,and magnetic field ***-dimensional particle-in-cell simulations are carried out to examine the kinetic evolution of SRS under low magnetic intensity of w_c/w_0<*** are two density regions distinguished in which the absolute growth of enveloped electrostatic waves and spectrum present quite different *** a relatively low-density plasma(ne~0.20 nc),the plasma wave presents typical absolute growth and the magnetic field alleviates linear *** in the plasma whose density is near the cut-off point(ne~0.23 nc),the magnetic field induces a spectral splitting of the backscattering and forward-scattering *** has been observed in simulations and verified by theoretical *** to this effect,the onset of reflectivity delays,and the plasma waves form high-frequency oscillation and periodic envelope *** split wavenumber Dk/k0 is proportional to the magnetic field intensity and plasma *** studies provide novel insight into the kinetic behavior of SRS in magnetized plasmas.

关键词： laser plasma interaction stimulated Raman scattering longitudinal magnetized plasma spectrum analysis electron plasma wave

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Ethylene and Spermidine in Wheat Grains in Relation to Starch Content and Granule Size Distribution Under Water Deficit

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Journal of Integrative Agriculture 2014年第10期13卷 2141-2153页

作者： yanG Wei-bing LI Yong yin yan-ping JIANG Wen-wen PENG Dian-liang CUI Zheng-yong yanG Dong-qing WANG Zhen-lin National Key Laboratory of Crop Biology Ministry of Science and Technology/Agronomy CollegeShandong Agricultural University

Two wheat cultivars （Triticum aestivum L.） were used to evaluate the effects ofpost-anthesis severe water deficit （SD） on starch content and granule size distribution and their relations with ethylene and spermidine （Spd）. Comparison to the well-watered （WW） treatment, SD led to lower Spd and higher 1-aminocylopropane-l-carboxylic acid （ACC） concentrations and ethylene evolution rate （EER） in grains at the critical stage of forming starch granules. Application of Spd or aminoethoxyvinylglycine （AVG） significantly reduced ACC concentration and EER and increased Spd concentration, while ethephon or methylglyoxal-bis （MGBG） had an opposite impact. The volume and surface area distribution of starch granules showed a bimodal curve, while the number distribution exhibited a unimodal curve. SD caused a marked drop in grain weight, grain number and starch content, also led to a significant reduction in the proportion （both by volume and by surface area） of B-type starch granules （〈10 Ixm）, with an increase in those of A-type starch granules （〉10 ~tm）. Application of Spd or AVG increased the proportion （both by volume and by surface area） of B-type starch granules under SD. Correlation analysis suggested that ethylene and Spd showed an antagonism relation in the formation of B-type granules. These results suggested that it would be good for the formation of B-type starch granules to have the physiological traits of higher Spd and lower ACC concentrations and ethylene emission under SD.

关键词： ethylene evolution rate 1-aminocylopropane-1-carboxylic acid spermidine severe water deficit wheat (Triticumaestivum L.)

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Implementing New Requirements of Passenger Station Construction and Building Excellent Passenger Station

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Chinese Railways 2022年第1期 48-53页

作者： JI Mingjun ENG Liyu yin yan Shanghai Branch China Railway Construction Engineering Group Shanghai Greatdragon International Design Co.Ltd.

In line with the new concept of building up a“well-connected,fullyi integrated,environment-friendlypassenger-oriented,economically-efficient,culturally-rich,intelligent and convenient”railway station and the construction requirements of"elaborate,fine and exquisite construction of excellent passenger stations" put forward by China State Railway Group ***.,design optimization and innovation are carried out in the construction of Nantongxi Railway Station and Pingtan Station in the aspects offunctional layout,decoration style,cultural enhancement,construction materials to be used,details treatment of the station buildings and the use of photoelectric technology,focusing on the consideration and research for furrther enhancing cultural confidence,deeply integrating into the regional features,inheriting historical context,innovating artistic expression,trying diversified integration,and exploring the use of science and technology in the contexl 0f the new era,and thus devoting to the construction of railway passenger stations in the new era which will meet the growing needs of the people for a better life.

关键词： railway station buildings Nantongxi Railway Station Pingtan Railway Station excellent passenger station station buildings in the new era

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