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A Short Text Classification Model for Electrical Equipment Defects Based on Contextual Features

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Wuhan University Journal of Natural Sciences 2022年第6期27卷 465-475页

作者： LI peipei ZENG Guohui HUANG Bo yin Ling SHI Zhicai HE Chuanpeng LIU Wei CHEN Yu School of Electrical and Electronic Engineering Shanghai University of Engineering ScienceShanghai 201620China CSG Smart Science&Technology Co LTDShanghai 201203China State Grid Shanghai Municipal Electric Power Company Shanghai 200122China

The defective information of substation equipment is usually recorded in the form of text. Due to the irregular spoken expressions of equipment inspectors, the defect information lacks sufficient contextual information and becomes more *** solve the problem of sparse data deficient of semantic features in classification process, a short text classification model for defects in electrical equipment that fuses contextual features is proposed. The model uses bi-directional long-short term memory in short text classification to obtain the contextual semantics of short text data. Also, the attention mechanism is introduced to assign weights to different information in the context. Meanwhile, this model optimizes the convolutional neural network parameters with the help of the genetic algorithm for extracting salient features. According to the experimental results, the model can effectively realize the classification of power equipment defect text. In addition, the model was tested on an automotive parts repair dataset provided by the project partners, thus enabling the effective application of the method in specific industrial scenarios.

关键词： short text classification genetic algorithm convolutional neural network attention mechanism

Synergistic effects of carbon cycle metabolism and photosynthesis in Chinese cabbage under salt stress

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Horticultural Plant Journal 2024年第2期10卷 461-472页

作者： Hao Liang Qiling Shi Xing Li peipei Gao Daling Feng Xiaomeng Zhang yin Lu Jingsen Yan Shuxing Shen Jianjun Zhao Wei Ma State Key Laboratory of North China Crop Improvement and Regulation Key Laboratory of Vegetable Germplasm Innovation and Utilization of HebeiCollaborative Innovation Center of Vegetable Industry in HebeiCollege of HorticultureHebei Agricultural UniversityBaodingHebei 071000China

Chinese cabbage(Brassica rapa ssp. pekinensis) has a long cultivation history and is one of the vegetable crops with the largest cultivation area in China. However, salt stress severely damages photosynthesis and hormone metabolism, nutritional balances, and results in ion toxicity in plants. To better understand the mechanisms of salt-induced growth inhibition in Chinese cabbage, RNA-seq and physiological index determination were conducted to explore the impacts of salt stress on carbon cycle metabolism and photosynthesis in Chinese cabbage. Here, we found that the number of thylakoids and grana lamellae and the content of starch granules and chlorophyll in the leaves of Chinese cabbage under salt stress showed a time-dependent response, first increasing and then decreasing. Chinese cabbage increased the transcript levels of genes related to the photosynthetic apparatus and carbon metabolism under salt stress, probably in an attempt to alleviate damage to the photosynthetic system and enhance CO_(2) fixation and energy metabolism. The transcription of genes related to starch and sucrose synthesis and degradation were also enhanced;this might have been an attempt to maintain intracellular osmotic pressure by increasing soluble sugar concentrations. Soluble sugars could also be used as potential reactive oxygen species(ROS) scavengers, in concert with peroxidase(POD)enzymes, to eliminate ROS that accumulate during metabolic processes. Our study characterizes the synergistic response network of carbon metabolism and photosynthesis under salt stress.

关键词： Chinese cabbage Salt stress Carbon metabolism Photosynthesis Chloroplast

Host-induced gene silencing of multiple pathogenic factors of Sclerotinia sclerotiorum confers resistance to Sclerotinia rot in Brassica napus

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The Crop Journal 2022年第3期10卷 661-671页

作者： Jian Wu Shengliang yin Li Lin Dongxiao Liu Sichao Ren Wenjing Zhang Wencheng Meng peipei Chen Qinfu Sun Yujie Fang Cunxu Wei Youping Wang Key Laboratory of Plant Functional Genomics of the Ministry of Education Yangzhou UniversityYangzhouChina Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding Yangzhou UniversityYangzhouChina

Sclerotinia sclerotiorum is generally considered one of the most economically damaging pathogens in oilseed rape(Brassica napus).Breeding for Sclerotinia resistance is challenging,as no immune germplasm available in *** is desirable to develop new breeding *** the present study,hostinduced gene silencing(HIGS),developed based on RNA interference(RNAi),was applied to protect *** from *** *** pathogenicity genes,the endo-polygalacturonase gene(Ss PG1),cellobiohydrolase gene(Ss CBH),and oxaloacetate acetylhydrolase gene(Ss OAH1),were chosen as HIGS ***-incubation of synthesized double-stranded RNAs(ds RNAs)with *** in liquid medium significantly reduced the transcript levels of the target *** to plant surfaces of ds RNA targeting the three genes conferred effective protection against *** transgenic *** plants expressing small interfering RNAs with sequence identity to Ss PG1,Ss CBH,and Ss OAH1 were *** transgenic *** prevented the expression of *** target genes,slowed pathogenicity-factor accumulation,impeded fungal growth,and suppressed appressorium formation,thereby conferring resistance to *** silencing of Ss PG1,Ss CBH,and Ss OAH1 by stable expression of a chimeric hairpin RNAi construct in *** led to enhanced protection phenotypes(with disease lesion size reduced by 36.8%–43.7%).We conclude that HIGS of pathogenic-factor genes of *** is a promising strategy for controlling Sclerotinia rot in oilseed rape.

关键词： Sclerotinia sclerotiorum Brassica napus SIGS HIGS RNAi

Origin and chromatin remodeling of young X/Y sex chromosomes in catfish with sexual plasticity

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National Science Review 2023年第2期10卷 91-106页

作者： Gaorui Gong Yang Xiong Shijun Xiao Xi-yin Li peipei Huang Qian Liao Qingqing Han Qiaohong Lin Cheng Dan Li Zhou Fan Ren Qi Zhou Jian-Fang Gui Jie Mei Hubei Hongshan Laboratory College of Fisheries Huazhong Agricultural University Jiaxing Key Laboratory for New Germplasm Breeding of Economic Mycology State Key Laboratory of Freshwater Ecology and Biotechnology Hubei Hongshan Laboratory Institute of HydrobiologyChinese Academy of Sciences University of the Chinese Academy of Sciences School of Animal Science and Nutritional Engineering Wuhan Polytechnic University MOE Laboratory of Biosystems Homeostasis & Protection Life Sciences InstituteZhejiang University

Assembly of a complete Y chromosome is a significant challenge in animals with an XX/XY sex-determination *** cently,we created YY-supermale yellow catfish by crossing XY males with sex-reversed XY females,providing a valuable model for Y-chromosome assembly and ***,we assembled highly homomorphic Y and X chromosomes by sequencing genomes of the YY supermale and XX female in yellow catfish,reve aling their nucleotide divergences with only less than 1% and with the same gene *** sex-determining region(SDR) was identified to locate within a physical distance of0.3 Mb by ***,the incipient sex chromosomes were revealed to originate via autosome-autosome fusion and were characterized by a highly rearranged region with an SDR downstream of the fusion *** found that the Y chromosome was at a very early stage of differentiation,as no clear evidence of evolutionary strata and classical structure features of recombination suppression for a rather late stage of Y-chromosome evolution were ***,a number of sex-antagonistic mutations and the accumulation of repetitive elements were discovered in the SDR,which might be the main driver of the initial establishment of recombination suppression between young X and Y ***,distinct three-dimensional chromatin organizations of the Y and X chromosomes were identified in the YY supermales and XX females,as the X chromosome exhibited denser chromatin structure than the Y chromosome,while they respectively have significantly spatial interactions with female-and male-related genes compared with other *** chromatin configuration of the sex chromosomes as well as the nucleus spatial organization of the XX neomale were remodeled after sex reversal and similar to those in YY supermales,and a male-specific loop containing the SDR was found in the open chromatin *** results elucidate the origin of young sex chromosomes and the chromatin remodel

关键词： Y chromosome sex-chromosome evolution chromosome fusion chromatin organization sexual reversal

High-temperature wheat leaf rust resistance gene Lr13 exhibits pleiotropic effects on hybrid necrosis

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Molecular Plant 2021年第7期14卷 1029-1032页

作者： Xiaocui Yan Miaomiao Li peipei Zhang Guihong yin Huaizhi Zhang Takele Weldu Gebrewahid Jianping Zhang Lingli Dong Daqun Liu Zhiyong Liu Zaifeng Li College of Plant Protection State Key Laboratory of North China Crop Improvement and RegulationHebei Agricultural UniversityBaodingHebei 071001China State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyThe Innovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China College of Agronomy Henan Agricultural UniversityZhengzhouHenan 450002China University of Chinese Academy of Sciences Beijing 100049China CSIRO Agriculture&Food GPO Box 1700CanberraACT 2601Australia

Dear Editor,Wheat is one of the most important staple food *** rust caused by Puccinia triticina Eriks.(Pt)is a destructive foliar disease that threatens world wheat *** wheat genes Necrosis 1(Ne1)and Necrosis 2(Ne2)on chromosome arms 5BL and 2BS,respectively,cause hybrid necrosis(Caldwell and Compton,1943;Chu et al.,2006).Ne2 shows extremely tight genetic linkage with leaf rust resistance gene Lr13(Zhang et al.,2016).We previously identified a temperature-sensitive leaf rust resistance gene LrZH22 in Chinese wheat cultivar Zhoumai 22(ZM,Figure 1A)that was effective against most Chinese Pt pathotypes(Wang et al.,2016)and shared an overlapping genetic interval with *** a separate study,a recessive early leaf senescence 1(els1)gene(Figure 1B)shared an overlapping interval with LrZH22/Lr13/Ne2(Chu et al.,2006;Wang et al.,2016;Zhang et al.,2016;Li et al.,2018).However,the function and relationship of LrZH22/Lr13/Ne2/els1 remained unknown for ***,we report the map-based cloning of LrZH22 and els1 and characterize their relationship with Lr13 and Ne2 in conjunction with a companion paper(Hewitt et al.t 2021)studying the same gene.

关键词： cultivar Wheat resistance

Lnc-RP5 Regulates the mi R-129-5p/Notch1/PFV Internal Promoter Axis to Promote the Expression of the Prototype Foamy Virus Transactivator Tas

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Virologica Sinica 2020年第1期35卷 73-82页

作者： Shanshan Xu Liujun Chen yinglian Tang peipei Yuan Jun Yan yingcheng Zheng Li Huang Zhi Li Yan Sun Song Han Jun yin Qin Pan Biwen Peng Xiaohua He Wanhong Liu Department of Allergy Zhongnan Hospital of Wuhan UniversityWuhan 430071China Department of Immunology and Hubei Province Key Laboratory of Allergy and Immunology School of Basic Medical SciencesWuhan UniversityWuhan 430071China Hubei Provincial Key Laboratory of Developmentally Originated Disease Wuhan 430071China College of Life Sciences Shanxi Normal UniversityXi’an 710062China Centre for Medical Research Wuhan UniversityWuhan 430071China

Prototype foamy virus(PFV)is a unique retrovirus that infects animals and humans and does not cause clinical *** noncoding RNAs(lncRNAs)are believed to exert multiple regulatory functions during viral ***,we utilized RNA sequencing(RNA-seq)to characterize and identify the lncRNA lnc-RP5-1086 D14.3.1-1:1(lnc-RP5),which is markedly decreased in PFV-infected ***,little is known about the function of lnc-RP5 during PFV *** this study,we identified lnc-RP5 as a regulator of the PFV transcriptional transactivator(Tas).Lnc-RP5 enhanced the activity of the PFV internal promoter(IP).The expression of PFV Tas was found to be promoted by ***,mi R-129-5 p was found to be involved in the lnc-RP5-mediated promotion of PFV IP activity,while the Notch1 protein suppressed the activity of PFV IP and the expression of *** results demonstrate that lnc-RP5 promotes the expression of PFV Tas through the miR-129-5 p/Notch1/PFV IP *** work provides evidence that host lnc RNAs can manipulate PFV replication by employing mi RNAs and proteins during an early viral infection.

关键词： Prototype foamy virus(PFV) lnc-RP5 Transactivator(Tas) Internal promoter miR-129-5p

心肌肥厚预适应小鼠模型的构建改良与无创评价

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中华心力衰竭和心肌病杂志（中英文） 2020年第2期4卷 114-121页

作者：吴剑代方杰黄家园李璇胡耀方殷培培杨春杰李冰玉张真中游洁芸王时俊邹云增复旦大学附属中山医院/生物医学研究院上海市心血管病研究所上海200032 南部战区空军医院肿瘤科广州510062 上海市东方医院(同济大学附属东方医院)心内科 200120

目的肥厚预适应(hypertrophic preconditioning,HP)是新近在小鼠模型上发现的一种抑制心肌肥厚的保护机制。但这一小鼠模型由于反复开胸缩窄/去缩窄主动脉,面临胸腔探查时间长、小鼠死亡率高等问题,制约了对HP进行在体水平的深入研究。... 详细信息

目的肥厚预适应(hypertrophic preconditioning,HP)是新近在小鼠模型上发现的一种抑制心肌肥厚的保护机制。但这一小鼠模型由于反复开胸缩窄/去缩窄主动脉,面临胸腔探查时间长、小鼠死亡率高等问题,制约了对HP进行在体水平的深入研究。我们拟对HP模型构建的关键环节进行改良,并使用超声生物显微镜无创评估心脏负荷程度,以确保模型的稳定高效建立。方法将雄性C57BL/6J小鼠(10~14周龄)分为肥厚预适应传统操作组(C‑HP组,N=40),肥厚预适应改良操作组(O‑HP组,N=40,留置长线头埋于胸廓皮下,使用显微镊解开缩窄线结),主动脉缩窄组(TAC组,N=20)及假手术组(Sham组,N=20)。各组最后一次术后4周,存活小鼠行超声生物显微镜以及心脏病理切片评估心脏肥大程度。超声检测室壁厚度和心腔内径。超声检查后对小鼠行安乐死取心脏,测量心脏重量/体重比,行病理切片检测心肌细胞横截面积和纤维化程度。结果在去缩窄和再缩窄过程中,O‑HP组较C‑HP组明显缩短手术时间,降低围手术期(从实验开始到手术结束6h)死亡率。再缩窄术后4周,O‑HP组小鼠存活率为67.5%(27/40),高于C‑HP组小鼠的37.5%(15/40),差别有统计学意义(P=0.007)。超声生物显微镜无创性检测主动脉缩窄处血流发现,O‑HP组与C‑HP组的缩窄处血流峰值在缩窄‑去缩窄‑再缩窄等关键环节的变化趋势类似,对HP模型进行改良不影响心脏承受的压力负荷水平。与Sham组小鼠对比,主动脉缩窄4周组(TAC 4W组)小鼠心室壁增厚、心功能受损、心肌细胞横截面积增大,纤维化增加,差别有统计学意义(P均<0.05)。主动脉再缩窄4周组(Re‑TAC 4W组)小鼠上述肥大反应明显减轻。此外,Re‑TAC 4W组小鼠与TAC 4W组小鼠相比,心房利钠肽及B型利钠肽表达减少,细胞外信号调节激酶(ERK1/2)激活程度明显减轻。结论我们成功构建优化并无创验证了一种心肌HP小鼠模型。该模型明显提高手术时效,降低小鼠死亡率,实现无创追踪心脏压力负荷状态,并初步揭示了ERK1/2可能是HP相关的重要信号因子,为心肌肥厚的早期干预和药物筛选研究提供了高效实用的动物模型。

关键词：压力超负荷肥厚预适应优化小鼠

Multiphenylthio Substituted Porphyrins

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Multiphenylthio Substituted Porphyrins

中国化学会第五届卟啉与酞菁学术研讨会

作者： peipei Jiang Ling Xu Mingbo Zhou Bangshao yin Jianxin Song Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education) Key Laboratory of Application and Assemble of Organic Functional Molecules of Hunan Province Hunan Normal University

Porphyrins are a group of aromatic molecules with various *** are partly due to their electron-rich core and therefore the ease of giving electrons in HOMO *** the other hand,both-SPh and-SR are well-known electron-donating functional *** make porphyrins owning higher electron density and thus stronger electron donors,we synthesized porphyrins bearing multiphenylthio *** from Chen and Zhang’s methods,we developed a very simple transition metal-free strategy for multiphenylthio porphyrins.β,β-,β,meso-,and β,meso,β-multiphenylthio porphyrins were achieved with excellent selectivity and good *** structures were characterized by H NMR and X-ray single crystal diffraction(Fig.1).

关键词： porphyrin multiphenylthio SN Ar reaction

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基于改进LHS模式的可靠性设计优化

机械强度 2011年第3期33卷 348-352页

作者：张培培尹子栋西北工业大学机电学院西安710072

在设计优化中,确定性优化由于没有考虑输入量的不确定性,其优化结果可能不可靠(不安全),因此基于可靠性的设计优化(reliability-based design optimization,RBDO)得到关注。然而可靠性设计优化计算量大,尤其对于高维问题。基于此,提出... 详细信息

在设计优化中,确定性优化由于没有考虑输入量的不确定性,其优化结果可能不可靠(不安全),因此基于可靠性的设计优化(reliability-based design optimization,RBDO)得到关注。然而可靠性设计优化计算量大,尤其对于高维问题。基于此,提出一种新方法——改进拉丁超立方体取样(Latin hypercube sampling,LHS)方法,该方法可利用先前迭代步骤已用的取样点,从而降低计算量。其中可靠性指数通过基于漫射近似(diffuse approximation,DA)的一阶可靠性方法(first-order reliability method,FORM)计算得到。最后用两个数学实例验证该方法可以极大地降低RBDO问题的计算量。

关键词：可靠性设计优化(reliability-based design optimization RBDO)拉丁超立方体取样一阶可靠性方法(first-order reliability method FORM)漫射近似(diffuse approximation DA)