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Induction of apoptosis and change of bcl-2 expression in macrophage Ana-1 cells by all-trans retinoic acid

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Cell Research 1996年第2期6卷 137-144页

作者： yin de ling XIU HAI REN SHI ZHONG BU YA LAN WU LI ZHEN JIANG ZHI JIANG WU WEI HU GANG PEI (Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China)(Correspondence: Dr. G. Pei, Shanghai Institute of Cell Biology Chine Shanghai Institute of Cell Biology Chinese Academy of Sciences Shanghai 200031 China.

Macrophage cells play an important role in the initiation and regulation of the immune response. All-trans retinoic acid (ATRA) and its natural and synthetic analogs (retinoids) affect a large number of biological *** , retinoids have been shown promise in the therapy and prevention of various cancers. However, many interesting questions related to the activities of retinoids remain to be answered: (Ⅰ) Molecular mechanisms by which retinoids exert their effects; (Ⅱ) why the clinical uses of retinoids give undesirable side effects of varying severity with a higher freqllency of blood system symptoms; (Ⅲ)little is known for its impacts on macrophage cells etc. We set up this experiment, therefore, to examine the apoptosis of ATRA on macrophage Ana-1 cell line. Apoptosis of the cells was quantitated, after staining cells with propidium iodide (PI), by both accounting nuclear condensation and flow cytometry. When the cells were treated with ATRA at or higher than 1 μM for more than 24 h, significant amount of the apoptotic cells was observed. Induction of apoptosis of Ana-1 cells by ATRA was in time- and dose-dependent manners, exhibiting the similar pattern as the apoptosis induced by actinomycin D (ACTD). ATRA treatment of Ana-1 cells also caused the changes of the mRNA levels of apoptosis-associated gene bcl-2, as detected by Northern blot analysis. The temporal changes of bcl-2 expression by ATRA was also parallel to that by ACTD. In conclusion,ATRA can induce apoptosis in macrophage cells, which may be helpful in understanding of immunological functions retinoids.

关键词： All-trans retinoic acid apoptosis Ana-1 cells bcl-2

Regulation and function of endoplasmic reticulum autophagy in neurodegenerative diseases

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Neural Regeneration Research 2025年第1期20卷 6-20页

作者： Xiu-Yun Zhao de-En Xu Ming-Lei Wu Ji-Chuan Liu Zi-ling Shi Quan-Hong Ma department of Neurology and Clinical Research Center of Neurological Disease the Second Affiliated Hospital of Soochow UniversitySuzhouJiangsu ProvinceChina Institute of Neuroscience&Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases Soochow UniversitySuzhouJiangsu ProvinceChina department of Neurology Jiangnan University Medical CenterWuxiJiangsu ProvinceChina

The endoplasmic reticulum,a key cellular organelle,regulates a wide variety of cellular *** reticulum autophagy,one of the quality control systems of the endoplasmic reticulum,plays a pivotal role in maintaining endoplasmic reticulum homeostasis by controlling endoplasmic reticulum turnover,remodeling,and *** this review,we briefly describe the endoplasmic reticulum quality control system,and subsequently focus on the role of endoplasmic reticulum autophagy,emphasizing the spatial and temporal mechanisms underlying the regulation of endoplasmic reticulum autophagy according to cellular *** also summarize the evidence relating to how defective or abnormal endoplasmic reticulum autophagy contributes to the pathogenesis of neurodegenerative *** summary,this review highlights the mechanisms associated with the regulation of endoplasmic reticulum autophagy and how they influence the pathophysiology of degenerative nerve *** review would help researchers to understand the roles and regulatory mechanisms of endoplasmic reticulum-phagy in neurodegenerative disorders.

关键词： autophagy endoplasmic reticulum endoplasmic reticulum autophagy endoplasmic reticulum quality control system endoplasmic reticulum receptors endoplasmic reticulum-associated degradation neurodegeneration neurodegenerative disease selective autophagy unfolded protein response

The emerging role of nitric oxide in the synaptic dysfunction of vascular dementia

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Neural Regeneration Research 2025年第2期20卷 402-415页

作者： Xiaorong Zhang Zhiying Chen yinyi Xiong Qin Zhou ling-Qiang Zhu Dan Liu department of Pathology Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina Jiujiang Clinical Precision Medicine Research Center JiujiangJiangxi ProvinceChina Center for Cognitive Science and Transdisciplinary Studies Jiujiang UniversityJiangxi ProvinceChina department of Neurology Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina department of Rehabilitation Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina department of Pathophysiology School of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

With an increase in global aging,the number of people affected by cerebrovascular diseases is also increasing,and the incidence of vascular dementia-closely related to cerebrovascular risk-is increasing at an epidemic ***,few therapeutic options exist that can markedly improve the cognitive impairment and prognosis of vascular dementia *** in Alzheimer’s disease and other neurological disorders,synaptic dysfunction is recognized as the main reason for cognitive *** oxide is one of the ubiquitous gaseous cellular messengers involved in multiple physiological and pathological processes of the central nervous ***,nitric oxide has been implicated in regulating synaptic plasticity and plays an important role in the pathogenesis of vascular *** review introduces in detail the emerging role of nitric oxide in physiological and pathological states of vascular dementia and summarizes the diverse effects of nitric oxide on different aspects of synaptic dysfunction,neuroinflammation,oxidative stress,and blood-brain barrier dysfunction that underlie the progress of vascular ***,we propose that targeting the nitric oxide-sGC-cGMP pathway using certain specific approaches may provide a novel therapeutic strategy for vascular dementia.

关键词： endoplasmic reticulum stress endothelial nitric oxide synthase gene therapy nitric oxide NO-sGC-cGMP pathway synaptic dysfunction vascular dementia

Acute and chronic excitotoxicity in ischemic stroke and late-onset Alzheimer's disease

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Neural Regeneration Research 2025年第7期20卷 1981-1988页

作者： Shan Ping Yu Emily Choi Michael QJiang ling Wei department of Anesthesiology Emory University School of MedicineAtlantaGAUSA Center for Visual and Neurocognitive Rehabilitation Atlanta Veterans Affairs Medical CenterDecaturGAUSA

Stroke and Alzheimer's disease are common neurological disorders and often occur in the same *** comorbidity of the two neurological disorders represents a grave health threat to older *** review presents a brief background of the development of novel concepts and their clinical *** activity of glutamatergic N-methyl-D-aspartate receptors and N-methyl-D-aspartate receptor-mediated Ca^(2+)influx is critical for neuronal *** ischemic insult induces prompt and excessive glutamate release and drastic increases of intracellular Ca^(2+)mainly via N-methyl-D-aspartate receptors,particularly of those at the extrasynaptic *** Ca^(2+)-evoked neuronal cell death in the ischemic core is dominated by necrosis within a few hours and days known as acute ***,mild but sustained Ca^(2+)increases under neurodegenerative conditions such as in the distant penumbra of the ischemic brain and early stages of Alzheimer's disease are not immediately toxic,but gradually set off deteriorating Ca^(2+)-dependent signals and neuronal cell loss mostly because of activation of programmed cell death *** on the Ca^(2+)hypothesis of Alzheimer's disease and recent advances,this Ca^(2+)-activated“silent”degenerative excitotoxicity evolves from years to decades and is recognized as a unique slow and chronic *** N-methyl-D-aspartate receptor subunit GluN3A,primarily at the extrasynaptic site,serves as a gatekeeper for the N-methyl-D-aspartate receptor activity and is neuroprotective against both acute and chronic *** stroke and Alzheimer's disease,therefore,share an N-methyl-D-aspartate receptor-and Ca^(2+)-mediated mechanism,although with much different time *** is thus proposed that early interventions to control Ca^(2+)homeostasis at the preclinical stage are pivotal for individuals who are susceptible to sporadic late-onset Alzheimer's disease and Alzheimer's disease-related

关键词： Ca^(2+)hypothesis cognitive deficits hyperactivity late-onset Alzheimer's disease neurodegeneration N-methyl-D-aspartate receptors N-methyl-D-aspartate receptor subunits pathogenesis preventive treatment

Assessment of Elasticity,Plasticity and Resistance to Machining-induced Damage of Porous Pre-sintered Zirconia Using Nanoindentation Techniques

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Journal of Materials Science & Technology 2016年第5期32卷 402-410页

作者： Abdur-Rasheed Alao ling yin Matter & Materials College of Science Technology & Engineering James Cook University

Porous pre-sintered zirconia is subject to white machining during which its elasticity, plasticity and resistance to machining-induced damage determine its machinability and final quality. This study used nanoindentation techniques and the Sakai＇s series elastic and plastic deformation model to extract the resistance to plastic deformation from the plane strain modulus and the contact hardness for presintered zirconia. The modulus and the resistance to plasticity were used to calculate the relative amount of elasticity and plasticity. The fracture energy and the normalized indentation absorbed energy were used to deconvolute the resistance to machining-induced cracking based on the Sakai-Nowak model. All properties were extracted at a 10 mN peak load and loading rates of 0.1-2 mN/s to determine the loading rate effects on these properties. We found that the resistance to plasticity and the resistance to machining-induced cracking were independent of the loading rate （ANOVA, p 〉 0.05）. The elastic and plastic displacements depended on the loading rate through power laws. This loading rate-dependent deformation behaviour was explained by the maximum shear stress generated underneath the indenter and the indentation energy. The plastic deformation components and the indentation absorbed energy at all loading rates were higher than the elastic deformation components and the elastic strain energy, respectively. Finally, we established the linkage among the pore structure, indentation behaviour and machinability of pre-sintered zirconia.

关键词： Elastic/plastic deformation Loading rate Nanoindentation Pre-sintered zirconia Resistance to machining-induced cracking Resistance to plasticity

Eigenfunctions of Five-Qubit XX Chain and Ground State Concurrence

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Communications in Theoretical Physics 2008年第12期50卷 1295-1298页

作者： ling yin-Sheng School of Physical Science and Technology Suzhou University Suzhou 215006 China

Use Jordan-Wigner transformation the eigenstates and eigenenergies of live qubits XX chain including external magnetic field are obtained. The concurrences Co,1 and Co,2 of ground state are obtained. For the ferromagnetic, when [（√5 - 1）/2]｜J｜〈 B ≤ ｜J｜, the values of C0,1 and C0,2 are the same. The C0,2 is easily broken by temperature.

关键词： XX chain entanglement Jordan-Wigner transformation concurrence

Discontinuous solid solutions of anthracene-phenanthrene:Thermodynamics and crystallization kinetics

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Particuology 2022年第1期20卷 68-76页

作者： Zaixiang Zhang ling Zhou Qiuxiang yin School of Chemical Engineering and Technology State Key Laboratory of Chemical EngineeringTianjin UniversityTianjin 300072People's Republic of China

We explored such issues as the formation mechanism,structure and propriety of the solid solutions of anthracene(ANT)-phenanthrene(PHE).Solution crystallization and solid-state grinding were employed to prepare solid solutions under different *** thermal behavior and PXRD scanning results revealed the formation of discontinuous solid solutions,whose melting points and crystal lattices varied linearly with mixed *** with Materials Studio,the formation possibility of solid solutions were investigated by evaluating the change of the *** crystal morphology of the solid solutions have a positive correlation with the change of the major ***,the solution crystallization process of solid solution were studied using the population balance model.

关键词： Discontinuous solid solutions Thermal proprieties Crystal structure Morphology Crystallization kinetics

Incremental harmonic balance method for periodic forced oscillation of a dielectric elastomer balloon

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Applied Mathematics and Mechanics(English Edition) 2020年第3期41卷 459-470页

作者： yin WANG ling ZHANG Jinxiong ZHOU State Key Laboratory Base of Eco-hydraulic Engineering in Arid Area Xi’an University of TechnologyXi’an 710048China State Key Laboratory for Strength and Vibration of Mechanical Structures Shaanxi Engineering Laboratory for Vibration Control of Aerospace StructuresSchool of AerospaceXi’an Jiaotong UniversityXi’an 710049China

Dielectric elastomer(de) is suitable in soft transducers for broad applications,among which many are subjected to dynamic loadings, either mechanical or electrical or both. The tuning behaviors of these de devices call for an efficient and reliable method to analyze the dynamic response of de. This remains to be a challenge since the resultant vibration equation of de, for example, the vibration of a de balloon considered here is highly nonlinear with higher-order power terms and time-dependent coefficients. Previous efforts toward this goal use largely the numerical integration method with the simple harmonic balance method as a supplement. The numerical integration and the simple harmonic balance method are inefficient for large parametric analysis or with difficulty in improving the solution accuracy. To overcome the weakness of these two methods,we describe formulations of the incremental harmonic balance(IHB) method for periodic forced solutions of such a unique system. Combined with an arc-length continuation technique, the proposed strategy can capture the whole solution branches, both stable and unstable, automatically with any desired accuracy.

关键词： dielectric elastomer nonlinear oscillation incremental harmonic method arc-length continuation

Char Plant:A de Novo Open Chromatin Region Prediction Tool for Plant Genomes

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Genomics, Proteomics & Bioinformatics 2021年第5期19卷 860-871页

作者： yin Shen ling-ling Chen Junxiang Gao Hubei Key Laboratory of Agricultural Bioinformatics College of InformaticsHuazhong Agricultural UniversityWuhan 430070China National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China

Chromatin accessibility is a highly informative structural feature for understanding gene transcription regulation,because it indicates the degree to which nuclear macromolecules such as proteins and RNAs can access chromosomal *** have shown that chromatin accessibility is highly dynamic during stress response,stimulus response,and developmental ***,physical access to chromosomal DNA in eukaryotes is highly ***,current technologies such as DNase-seq,ATAC-seq,and FAIRE-seq reveal only a portion of the open chromatin regions(OCRs)present in a given ***,the genome-wide distribution of OCRs remains *** this study,we developed a bioinformatics tool called Char Plant for the de novo prediction of OCRs in plant *** develop this tool,we constructed a three-layer convolutional neural network(CNN)and subsequently trained the CNN using DNase-seq and ATACseq datasets of four plant *** model simultaneously learns the sequence motifs and regulatory logics,which are jointly used to determine DNA *** of these steps are integrated into Char Plant,which can be run using a simple command *** results of data analysis using Char Plant in this study demonstrate its prediction power and computational *** our knowledge,Char Plant is the first de novo prediction tool that can identify potential OCRs in the whole *** source code of Char Plant and supporting files are freely available from https://***/yin-Shen/Char Plant.

关键词： Open chromatin region Chromatin accessibility Convolutional neural network de novo prediction Plant genome