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Chinese Medical Journal 2012年第13期125卷 2371-2377页

作者： LI yi-jia WANG Huan-ling LI Tai-sheng Department of Infectious Diseases Peking Union Medical College HospitalPeking Union Medical CollegeChinese Academy of Medical SciencesBeijing 100730China

Objective This review discusses progress in the studies of hepatitis B virus （HBV）/human immunodeficiency virus （HIV） coinfection and focuses on the interaction among HIV infection, chronic HBV infection, and host immunity. Data sources Data and studies pubJished mainly from 2008 to 2011 were selected using PubMed. Study selection Original articles and critical reviews concerning HBV/HIV coinfection and HBV and HIV pathogenesis were selected. Results HIV may accelerate HBV progression by lowering CD4 count, weakening HBV-specific immunity, ＂enriching＂ HBV mutants, causing immune activation, etc. On the other hand, HBV may enhance HIV replication by activating HIV long terminal repeat （LTR） with X protein （HBX） and cause immune activation in synergy with HIV. Paradoxically, HBV may also inhibit HIV dissemination via dendritic cells. Conclusions The interaction among HIV, HBV, and host immunity remains poorly understood. Further research is warranted to elucidate the detailed molecular mechanisms and to translate these mechanisms into clinical practice.

关键词： human immunodeficiency virus hepatitis B virus immunity

Case of metachronous bilateral isolated adrenal metastasis from colorectal adenocarcinoma and review of the literature

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World Journal of Gastroenterology 2016年第14期22卷 3879-3884页

作者： Yu-yi Liu zhi-hui Chen Er-tao Zhai Jie Yang Jian-bo Xu Shi-rong Cai Wu Song Department of Gastrointestinal Surgery the First Affiliated Hospital Sun Yat-sen University

rarely has a solitary, metachronous bilateral adrenal metastasis of colorectal cancer been reported. We depict a 41-year-old man who underwent sigmoid colon cancer radical surgery followed by adjuvant chemotherapy for alocally ulcerative sigmoid adenocarcinoma with metachronous bilateral adrenal metastasis revealed by a computed tomography scan. histopathological examination showed adenocarcinoma, compatible with metastasis from the rectal cancer. the level of serum carcinoembryonic antigen had indicative significance for the presence of adrenal metastasis in the reported series. We performed a literature analysis related to this pathological characteristic and attach importance to consistent, vigilant radiological surveillance of the adrenal glands in the patients' follow up for colorectal cancer with or without subsequent adrenal metastasis.

关键词： Adrenal gland bilateral Colorectal cancer metastasis metachronous

离子抑制色谱法测定复方氨基酸注射液(18AA-V)中木糖醇的含量

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中南药学 2024年第10期22卷 2754-2757页

作者：肖菁左利民谷永升赵婷连晓芳刘惠一王蓉蓉周怡山广志湖南省药品检验检测研究院长沙410001 中国医学科学院医药生物技术研究所北京100050 新疆医科大学药学院乌鲁木齐830017 国家药典委员会北京100061

目的建立离子抑制色谱法测定复方氨基酸注射液(18AA-V)中木糖醇的含量。方法采用HyperREZ XP Carbohydrate H+色谱柱(7.7 mm×300 mm,8μm),以12.5×10^(-3) mol·L^(-1)硫酸溶液为流动相,等度洗脱,流速为0.5 mL·min-1,柱温为55℃,检... 详细信息

目的建立离子抑制色谱法测定复方氨基酸注射液(18AA-V)中木糖醇的含量。方法采用HyperREZ XP Carbohydrate H+色谱柱(7.7 mm×300 mm,8μm),以12.5×10^(-3) mol·L^(-1)硫酸溶液为流动相,等度洗脱,流速为0.5 mL·min-1,柱温为55℃,检测波长为200 nm。结果木糖醇峰和相邻色谱峰分离度良好,在5.002~20.01 mg·mL^(-1)内与峰面积线性良好(r=0.9997,n=5),精密度、重复性、稳定性试验的RSD均<1.0%,低、中、高浓度的回收率分别为99.10%、97.86%和96.82%,RSD分别为1.9%、1.7%和0.5%(n=3)。3批复方氨基酸注射液(18AA-V)中木糖醇含量分别为标示量的96.96%、97.25%、97.32%。结论该方法可有效解决氨基酸成分的干扰,快速简便、专属性强、准确度高,可用于复方氨基酸注射液(18AA-V)中木糖醇的含量测定。

关键词：离子抑制色谱法复方氨基酸注射液(18AA-V) 木糖醇含量测定

Identification and characterization of two morphologically distinct stem cell subpopulations from human urine samples

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Science China(Life Sciences) 2020年第5期63卷 712-723页

作者： An-Jing Chen Jin-Kui Pi Jun-Gen Hu yi-Zhou Huang Hong-Wei Gao Sheng-Fu Li Jesse Li-ling Hui-Qi Xie Laboratory of Stem Cell and Tissue Engineering State Key Laboratory of Biotherapy and Cancer CenterWest China HospitalSichuan University and Collaborative Innovation Center of BiotherapyChengdu 610041China Key Laboratory of Transplant Engineering and Immunology of Ministry of Health West China HospitalSichuan UniversityChengdu 610041China

Urine-derived stem cells(USCs)have shown potentials for the treatment of skeletal and urological *** on published literature and our own data,USCs consist of heterogeneous populations of *** this paper,we identify and characterize two morphologically distinct subpopulations of USCs from human urine samples,named as spindle-shaped USCs(SS-USCs)and rice-shaped USCs(RS-USCs)*** two subpopulations showed similar clone-forming efficiency,while SS-USCs featured faster proliferation,higher motility,and greater potential for osteogenic and adipogenic differentiation,RS-USCs showed greater potential for chondrogenic ***5F1 was strongly expressed in both subpopulations,but MYC was weakly *** subpopulations showed similar patterns of CD24,CD29,CD34,CD44,CD73,CD90 and CD105 expression,while a higher percentage of RS-USCs were positive for ***-USCs were positive for VIM,weakly positive for SLC12A1 and UMOD,and negative for KRT18,NPHS1,AQP1 and AQP2,indicating a renal mesenchyme origin;while RSUSCs are positive for VIM,partially positive for KRT18,NPHS1,AQP1,SLC12A1 and UMOD,and negative for AQP2,indicating a nephron tubule *** above results can facilitate understanding of the biological characteristics of subpopulations of USCs,and provide a basis for further research and applications of such cells.

关键词： characterization cell subpopulation identification tissue origin urine-derived stem cells

基于白光中子源的^(197)Au中子辐射俘获截面测量及共振参数分析

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物理学报 2024年第7期73卷 104-113页

作者：罗淏天张奇玮栾广源王晓宇邹翀任杰阮锡超贺国珠鲍杰孙琪黄翰雄王朝辉吴鸿毅顾旻皓余滔解立坤陈永浩安琪白怀勇鲍煜曹平陈昊磊陈琪萍陈裕凯陈朕崔增琪樊瑞睿封常青高可庆韩长材韩子杰何泳成洪杨黄蔚玲黄锡汝季筱璐吉旭阳蒋伟江浩雨姜智杰敬罕涛康玲康明涛李波李超李嘉雯李论李强李晓李样刘荣刘树彬刘星言穆奇丽宁常军齐斌斌任智洲宋英鹏宋朝晖孙虹孙康孙晓阳孙志嘉谭志新唐洪庆唐靖宇唐新懿田斌斌王丽娇王鹏程王琦王涛峰文杰温中伟吴青彪吴晓光吴煊羊奕伟易晗于莉于永积张国辉张林浩张显鹏张玉亮张志永赵豫斌周路平周祖英朱丹阳朱科军朱鹏朱兴华中国原子能科学研究院核数据重点实验室北京102413 中国科学院高能物理研究所北京100049 散裂中子源科学中心东莞523803 核探测与核电子学国家重点实验室北京100049 中国科学技术大学近代物理系核探测与核电子学国家重点实验室合肥230026 北京大学物理学院核物理与核技术国家重点实验室北京100871 中国工程物理研究院核物理与化学研究所绵阳621900 西北核技术研究院西安710024 中国科学院大学北京100049 中国科学技术大学工程与应用物理系合肥230026 北京航空航天大学物理学院北京100083 华能山东石岛湾核电有限公司荣成264312

中子辐射俘获反应在反应堆运行、核装置设计及核天体物理研究中起重要的作用.4πBaF_(2)探测装置有着高时间分辨能力、低中子灵敏度、高探测效率等优点,适合开展中子辐射俘获反应截面数据的测量.中国原子能科学研究院核数据重点实验室... 详细信息

中子辐射俘获反应在反应堆运行、核装置设计及核天体物理研究中起重要的作用.4πBaF_(2)探测装置有着高时间分辨能力、低中子灵敏度、高探测效率等优点,适合开展中子辐射俘获反应截面数据的测量.中国原子能科学研究院核数据重点实验室建立了伽马全吸收装置(Gamma total absorption facility,GTAF),该装置用28块六棱BaF_(2)晶体和12块五棱BaF_(2)晶体构成了外径25 cm,内径10 cm的球壳,覆盖了95.2%的立体角.利用GTAF在中国散裂中子源Back-n束线上,测量了197Au(n,γ)的反应截面数据.测量数据通过能量筛选、PSD方法、晶体多重性筛选进行了初步本底扣除,随后结合对^(nat)C及空样品的测量数据对本底进行了分析及扣除,获得了197Au俘获反应的产额,利用SAMMY程序拟合得到了^(197)Au在1—100 e V的共振能量、中子共振宽度和伽马共振宽度参数.实验测量结果与ENDF/B-VIII.0数据库符合良好,其共振参数存在一定差异,分析原因可能与GTAF能量分辨率、Back-n的中子能谱测量精度、以及实验本底扣除方法相关,这也是下一步工作的重点.

关键词：中子辐射俘获截面共振参数白光中子源伽马全吸收装置

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Houttuynia cordata polysaccharide alleviated intestinal injury and modulated intestinal microbiota in H1N1 virus infected mice

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Chinese Journal of Natural Medicines 2019年第3期17卷 187-197页

作者： CHEN Mei-Yu LI Hong LU Xiao-Xiao ling Li-Jun WENG Hong-Bo SUN Wei CHEN Dao-Feng ZHANG Yun-yi Department of Pharmacology School of Pharmacy Fudan University Department of Pharmacognosy School of Pharmacy Fudan University

Houttuynia cordata polysaccharide(HCP) is extracted from Houttuynia cordata, a key traditional Chinese medicine. The study was to investigate the effects of HCP on intestinal barrier and microbiota in H1N1 virus infected mice. Mice were infected with H1N1 virus and orally administrated HCP at a dosage of 40 mg×kg^(–1)×d^(–1). H1N1 infection caused pulmonary and intestinal injury and gut microbiota imbalance. HCP significantly suppressed the expression of hypoxia inducible factor-1α and decreased mucosubstances in goblet cells, but restored the level of zonula occludens-1 in intestine. HCP also reversed the composition change of intestinal microbiota caused by H1N1 infection, with significantly reduced relative abundances of Vibrio and Bacillus, the pathogenic bacterial genera. Furthermore, HCP rebalanced the gut microbiota and restored the intestinal homeostasis to some degree. The inhibition of inflammation was associated with the reduced level of Toll-like receptors and interleukin-1β in intestine, as well as the increased production of interleukin-10. Oral administration of HCP alleviated lung injury and intestinal dysfunction caused by H1N1 infection. HCP may gain systemic treatment by local acting on intestine and microbiota. This study proved the high-value application of HCP.

关键词： H1N1 influenza virus Houttuynia cordata Inflammation Intestinal Barrier Microbiota Polysaccharide

术前剂量密集TC治疗联合广泛性子宫切除术及盆腔淋巴结清扫在局部晚期宫颈癌患者中的应用研究

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中国性科学 2024年第1期33卷 119-124页

作者：勾晓娟王亚娟韩一魏玲李红霞霍志平河北中石油中心医院河北廊坊065000

目的探讨术前剂量密集卡铂+紫杉醇(TC)治疗联合广泛性子宫切除术及盆腔淋巴结清扫在局部晚期宫颈癌治疗中效果。方法选取2020年1月至2022年1月在河北中石油中心医院治疗的100例局部晚期宫颈癌患者作为研究对象,根据患者最终选取的治疗... 详细信息

目的探讨术前剂量密集卡铂+紫杉醇(TC)治疗联合广泛性子宫切除术及盆腔淋巴结清扫在局部晚期宫颈癌治疗中效果。方法选取2020年1月至2022年1月在河北中石油中心医院治疗的100例局部晚期宫颈癌患者作为研究对象,根据患者最终选取的治疗方案将患者分为观察组(n=50)和对照组(n=50)。两组均给予广泛性子宫切除术及盆腔淋巴结清扫,其中观察组术前给予剂量密集TC治疗,对照组术前给予常规TC治疗。观察并比较两组围手术期指标、化疗疗效、不良反应、世界卫生组织生存质量测定量表简表(WHOQOL-BREF)评分、无进展生存时间和总生存期。结果观察组手术时间为(210.03±41.16)min,显著短于对照组(P0.05)。观察组化疗疗效优于对照组,其客观有效率(ORR)为82.00%,显著高于对照组(P0.05)。观察组治疗后3个月WHOQOL-BREF生理领域评分为(10.84±1.69)分,显著高于对照组(P0.05)。观察组中位无进展生存时间为26.00个月(95%CI:23.37~28.64),显著长于对照组(P0.05)。结论术前剂量密集TC治疗联合广泛性子宫切除术及盆腔淋巴结清扫在局部晚期宫颈癌治疗中有较好的效果,可改善患者生存质量及延长无进展生存时间。

关键词：剂量密集卡铂+紫杉醇广泛性子宫切除术盆腔淋巴结清扫局部晚期宫颈癌治疗效果

Recombinant adeno-associated virus carrying thymosin β4 suppresses experimental colitis in mice

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World Journal of Gastroenterology 2017年第2期23卷 242-255页

作者： Xiao-Yan Zheng yi-Fei Lv Shuang Li Qian Li Qian-Nan Zhang Xue-Ting Zhang zhi-Ming Hao Department of Rheumatology The First Affiliated Hospital of Xi’an Jiaotong University Department of Gastroenterology Shaanxi Provincial People’s Hospital Department of Gastroenterology The First Affiliated Hospital of Xi’an Jiaotong University

AIMTo investigate the protective effect of a recombinant adeno-associated virus carrying thymosin β4 (AAV-Tβ4) on murine colitis via intracolonic ***-Tβ4 was prepared and intracolonically used to mediate the secretory expression of Tβ4 in mouse colons. Dextran sulfate sodium (DSS) was applied to induce the murine ulcerative colitis, and 2,4,6-trinitrobenzene sulfonic acid (TNBS) was used to establish a mouse colitis model resembling Crohn’s disease. The disease severity and colon injuries were observed and graded to reveal the effects of AAV-Tβ4 on colitis. The activities of myeloperoxidase (MPO) and superoxide dismutase (SOD) and the content of malondialdehyde (MDA) were determined using biochemical assays. Colonic levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-10 were measured using ELISA, and mucosal epithelial cell apoptosis and proliferation were detected by TUNEL assay and immunochemistry, *** AAVs efficiently delivered LacZ and Tβ4 into the colonic tissues of the mice, and AAV-Tβ4 led to a strong expression of Tβ4 in mouse colons. In both the DSS and TNBS colitis models, AAV-Tβ4-treated mice displayed distinctly attenuated colon injuries and reduced apoptosis rate of colonic mucosal epithelia. AAV-Tβ4 significantly reduced inflammatory cell infiltrations and relieved oxidative stress in the inflamed colons of the mice, as evidenced by decreases in MPO activity and MDA content and increases in SOD activity. AAV-Tβ4 also modulated colonic TNF-α, IL-1β and IL-10 levels and suppressed the compensatory proliferation of colonic epithelial cells in DSS- and TNBS-treated ***β4 exerts a protective effect on murin

关键词： Thymosin β 4 Mice Colitis Dextran sulfate sodium 2 4 6-trinitrobenzene sulfonic acid

18β-glycyrrhetinic acid regulates mitochondrial ribosomal protein L35-associated apoptosis signaling pathways to inhibit proliferation of gastric carcinoma cells

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World Journal of Gastroenterology 2022年第22期28卷 2437-2456页

作者： ling Yuan yi Yang Xia Li Xin Zhou Yu-Hua Du Wen-Jing Liu Lei Zhang Lei Yu Ting-Ting Ma Jia-Xin Li Yan Chen yi Nan College of Pharmacy Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Traditional Chinese Medicine College Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Key Laboratory of Hui Ethnic Medicine Modernization of Ministry of Education Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Department of Infectious Diseases The Fourth Hospital of Harbin Medical UniversityHarbin 150001Heilongjiang ProvinceChina

BACKGROUND Gastric carcinoma(GC)is a common gastrointestinal malignancy *** on the cancer-related mortality,the current prevention and treatment strategies for GC still show poor clinical ***,it is important to find effective drug treatment *** To explore the mechanism by which 18β-glycyrrhetinic acid(18β-GRA)regulates mitochondrial ribosomal protein L35(MRPL35)related signal proteins to inhibit the proliferation of GC *** Cell counting kit-8 assay was used to detect the effects of 18β-GRA on the survival rate of human normal gastric mucosal cell line GES-1 and the proliferation of GC cell lines MGC80-3 and *** apoptosis and cell cycle were assessed by flow *** invasion and migration were evaluated by Transwell assay,and cell scratch test was used to detect cell ***,a tumor model was established by hypodermic injection of 2.5×106 BGC-823 cells at the selected positions of BALB/c nude mice to determine the effect of 18β-GRA on GC cell proliferation,and quantitative reverse transcription-polymerase chain reaction(qRT-PCR)was used to detect MRPL35 expression in the engrafted tumors in *** used the term tandem mass tag(TMT)labeling combined with liquid chromatography–tandem mass spectrometry to screen for differentially expressed proteins(DEPs)extracted from GC cells and control cells after 18β-GRA intervention.A detailed bioinformatics analysis of these DEPs was performed,including Gene Ontology annotation and enrichment analysis,Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis,and so ***,STRING database(https://***/)was used to predict proteinprotein interaction(PPI)relationships and Western blot was used to detect the expression of proteins of interest in GC *** The results indicated that 18β-GRA could inhibit the proliferation of GC cells in a dose-and timedependent *** could induce GC cell apoptosis and arrest the cell cycle at G

关键词： Gastric carcinoma 18β-glycyrrhetinic acid Mitochondrial ribosomal protein L35 Proliferation Invasion Apoptosis

Nucleic acid detection in the diagnosis and prevention of schistosomiasis

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Infectious Diseases of Poverty 2016年第1期5卷 230-240页

作者： Ping He Lan-gui Song Hui Xie Jin-yi Liang Dong-ya Yuan Zhong-dao Wu zhi-yue Lv Department of Parasitology Zhongshan School of MedicineSun Yat-sen UniversityGuangzhou 510080China Key Laboratory of Tropical Disease Control Ministry of EducationGuangzhou 510080China Department of Pathogenic Biology Medical SchoolXizang Minzu UniversityXianyang 712082China

Schistosomiasis is an important zoonotic parasitic disease that causes serious harms to humans and *** and diagnosis play key roles in schistosomiasis control,however,current techniques for surveillance and diagnosis of the disease have *** genome data for parasites are increasing,novel techniques for detection incorporating nucleotide sequences are receiving widespread *** sensitive,specific,and rapid detection methods are particularly important in the diagnosis of low-grade and early infections,and may prove to have clinical *** paper reviews the progress of nucleic acid detection in the diagnosis and prevention of schistosomiasis,including such aspects as the selection of target genes,and development and application of nucleic acid detection methods.

关键词： Schistosoma japonicum S.mansoni S.haematobium Diagnosis Nucleic acid detection