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Effects of Silver Nanoparticles on Denitrification and Associated N_(2)O Release in Estuarine and Marine Sediments

Journal of Ocean University of China 2022年第1期21卷 131-140页

作者： SUN Pengfei LI Kuiran yi shaokui LI Hui CHEN Xi Fourth Institute of Oceanography Ministry of Natural ResourcesBeihai 536000China College of Environmental Science and Engineering Ocean University of ChinaQingdao 266100China College of Marine Life Science Ocean University of ChinaQingdao 266003China College of Life Sciences Huzhou UniversityHuzhou 313000China

Silver nanoparticles(AgNPs)have been widely used in medicine and consumer *** it enters the river in dif-ferent ways,then finally converges to the ocean through the *** polution can affect NO_(2)−and N_(2)O production by denitri-fiers in aquatic *** effects of AgNPs on denitrification activity,nitrogen transformation and nitrous oxide(N_(2)O)emission were investigated in Dagu River Estuary(DRE)and Jiaozhou Bay(JZB).The results showed that the potential denitrification activity(PDA),NO_(3)−and NO_(2)−reduction rates decreased with an increase of AgNPs concentration in DRE and ***,the N_(2)O ac-cumulation was significantly increased at AgNPs concentrations above 5 mg kg^(−1)in both areas,and the accumulation rate was greater in estuary than in bay(P0.05)in DRE and ***,the relative abundance of narG,nirS and nosZ gene in two areas decreased with the increase of AgNPs concentration,and the negative effect of AgNPs varied in order:nosZ>nirS>narG,inferring that the expression of denitrifying related genes could be significantly and differently inhibited by AgNPs ***,this study demon-strated that the inhibitory effect of AgNPs on different denitrification process,which may lead to the increase of inorganic nitrogen accumulation and N_(2)O *** study provides a scientific basis for the further studies of AgNPs on the ecological impact mechanism and environmental effects of offshore sedimentary environment.

关键词： nitrous oxide silver nanoparticles denitrification estuarine and marine sediments

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Heterosis and combining ability evaluation for growth traits of blunt snout bream(Megalobrama amblycephala)when crossbreeding three strains

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Chinese Science Bulletin 2014年第9期59卷 857-864页

作者： Wei Luo Cong Zeng shaokui yi Nicholas Robinson Weimin Wang Zexia Gao College of Fisheries Key Laboratory of Agricultural Animal Genetics Breeding and Reproduction of Ministry of Education/Key Laboratory of Freshwater Animal Breeding Ministry of Agriculture Huazhong Agricultural University Nofima PO Box 5010 1432 s Norway Collaborative Innovation Center for Healthy Freshwater Aquaculture of Hubei Province

The blunt snout bream(Megalobrama amblycephala)is a major aquaculture species in the Chinese freshwater polyculture *** this study,complete diallel crossing of three strains(i.e.,Liangzi[LZ],Poyang[PY]and Yuni[YN])was used to evaluate the combining ability and heterosis effect of intraspecific crossbreeding on the growth performance(i.e.,body weight,total length,body length,body height,and Fulton’s coefficient of condition).The offspring produced from the three strains were reared in a communal pond for 20 months,and nine microsatellites were used to assess their *** the749 offspring,708(94.53%)could be assigned directly to a single parental *** differences were observed in body weight(BW)among the different combinations,indicating that the combining ability of BW from each strain was significantly *** general combining ability(GCA)of BW from the sire was much higher than that from the *** GCA for the dam and sire of the LZ and YN were the largest and the smallest,*** special combining ability of YN$9 PY#was the largest,and YN$9 PY#showed significantly positive heterosis effects on all F1growth traits,except for Fulton’s condition coefficient(K)(P\0.05).In addition,significant positive linear correlations were found between the mean BW of corresponding progeny and the H E of the parental generation(y=600.7x-29.472,r=0.8651,P=0.0026),H O(y=1206.9x-518.14,r=0.7436,P=0.0216),and the polymorphism information content(y=1021.3x-331.31,r=0.8245,P=0.0063)as determined by a correlative *** findings obtained from the study will be important for future considerations of *** stock improvement programs.

关键词：杂种优势效应特殊配合力生长性状双列杂交团头鲂平均体重评价线性相关关系

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Isolation,identification,and virulence gene analysis of pathogenic Aeromonas dhakensis in Macrobrachium rosenbergii and histopathological observation

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Journal of Oceanology and Limnology 2024年第2期42卷 664-675页

作者： Xin PENG Haihui TU Xinyi YAO Xuan LAN Zhenxiao ZHONG Jinping LUO Qiongying TANG shaokui yi Zhenglong XIA Guoliang YANG Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development College of Life SciencesHuzhou UniversityHuzhou 313000China Jiangsu Shufeng Prawn Breeding Co. Ltd.Gaoyou 225654China

To identify the cause of mass mortality of adult Macrobrachium rosenbergii in a farm in Gaoyou City,Jiangsu Province,China,a dominant strain named DKQ-1 was isolated from the hepatopancreas of dying *** and identified as Aeromonas dhakensis by purification culture,biochemical characterization,and 16S rRNA and gyrB gene sequence *** results of the challenge test revealed that the strain was highly pathogenic and the 50%lethal dose(LD_(50))in 72 h to *** was 1.54×10^(5)CFU/*** amplification results of virulence genes show that strain DKQ-1 carried 9 virulence genes,including ascV,aexT,aer,act,lip,ompAI,gcaT,acg,and exu,supporting the strong virulence of strain DKQ-1 to *** observation of the hepatopancreas,gills,and intestines indicated that DKQ-1 injection into *** could cause serious tissue damage,which further supported the strong virulence of this *** addition,a drug susceptibility test revealed that strain DKQ-1 was sensitive to 16 kinds of antibiotics,resistant to 9 kinds of antibiotics,and had intermediate resistance to spectinomycin and *** study is the first report of *** isolated from *** and provided a reference for the pathogen identification of bacterial diseases in ***,and for the prevention and treatment caused by ***.

关键词： Aeromonas dhakensis histopathology virulence gene gyrB drug susceptibility test Macrobrachium rosenbergii

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Isolation,identification,and evaluation of intestinal bacteria in Macrobrachium rosenbergii

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Journal of Oceanology and Limnology 2024年第5期42卷 1710-1721页

作者： Xiuxin ZHAO Jinping LUO Peimin LIU Hao HUANG Zhenheng CHENG Xin PENG Qiongying TANG Guoliang YANG shaokui yi Quanxin GAO Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development Key Laboratory of Aquatic Animal Genetic Breeding and NutritionChinese Academy of Fishery SciencesCollege of Life ScienceHuzhou UniversityHuzhou 313000China Jiangsu Shufeng Prawn Breeding Co.Ltd. Gaoyou 225654China

Probiotics are live microbial food supplements that have been shown to have beneficial effects on animal *** probiotic bacteria have long been used for their proposed health promoting properties and have become a hot research topic in growth improvement in *** endogenous probiotic bacteria from intestines of Macrobrachium rosenbergii(giant river prawn)was explored for their probiotic potential,from which 367 bacterial strains were isolated from the intestine of *** 16 S rDNA sequence analysis,234 isolates were identified as Lactococcus garvieae,which accounted for 63.76%of the total number of culturable intestinal bacteria,suggesting that this bacterium was the main component of the ***,to reveal the probiotic properties of ***,this isolated bacterial strain was characterized morphologically,physiologically,and *** enzyme production capacity,bacteriostatic activity,and resistance to acid,high temperature,and pH,were *** vitro experiments showed that the ***(No.C 6 a 2)had a fast growth rate and entered the logarithmic phase ***,it had characteristics of acid-production and resistance,enzyme-producing capacity,and strong antibacterial activity against pathogenic Staphylococc us aureus,Aeromonas hydrophila,and Aeromonas ***,it lacked the ability to tolerate high *** results provide novel data to deepen our understanding of the intestinal bacteria structure of *** and valuable information for probiotic screening and the application for ***.

关键词： Macrobrachium rosenbergii intestinal bacteria probiotic Lactococcus garvieae

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Genetic parameter evaluation for growth and cold resistance traits of the giant freshwater prawn Macrobrachium rosenbergii

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Journal of Oceanology and Limnology 2024年第2期42卷 609-619页

作者： Haihui TU Qianqian XING Zhenxiao ZHONG Qiongying TANG shaokui yi Zhenglong XIA Miaoying CAI Jingfen LI Quanxin GAO Guoliang YANG Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development College of Life SciencesHuzhou UniversityHuzhou 313000China Fengjiashan Reservoir Administration Bureau Baoji 721300China Jiangsu Shufeng Prawn Breeding Co.Ltd. Gaoyou 225654China

The giant freshwater prawn Macrobrachium rosenbergii distributed from tropical to subtropical regions,is a warm-water species,and its survival temperature is 14-35°C,which greatly limits its culture cycle and culture area in ***,it is urgent to cultivate a new high quality,high yield variety with improved cold-resistance,but the genetic parameters for cold-resistance traits are unknown in *** this study,the cold-resistance of adult *** populations was tested using the indoor artificial cooling *** were selected from 139 families of Shufeng G3 generation and cultured for 200 days.A linear mixed model was constructed by ASReml-R to evaluate the genetic parameters of the cold-resistance trait(cooling degree hours,CDH)and growth traits(body weight,BW,and body length,BL)based on the restricted maximum likelihood(REML)*** results show that the heritability of CDH was low(0.12±0.05),while the growth traits(BW and BL)had low to moderate heritability,with 0.20±0.06 for BW and 0.06±0.04 for *** phenotypic and genetic correlation between BW and BL was significantly positive,but significantly negative phenotypic and genetic correlations were detected between CDH and BW and between CDH and ***,the analysis of the differences between cold-resistance and phenotypic traits showed that the female reproductive status,exoskeleton hardness and claw number of adult prawns had a great influence on the cold-resistance of ***(P<0.05),indicating that adults with claws and hard exoskeletons are preferred as parents in subsequent breeding *** present results can be attributed to the selection and breeding of a new cold-resistant variety of ***.

关键词： cold-resistance growth traits heritability correlation Macrobrachium rosenbergii

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我国七大流域大鳞副泥鳅形态学比较分析

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华中农业大学学报 2021年第3期40卷 230-237页

作者：陈鸿禄易少奎李军涛王卫民华中农业大学水产学院/农业动物遗传育种与繁育教育部重点实验室/农业农村部淡水生物繁育重点实验室武汉430070 湖州师范学院生命科学学院湖州313000 中国热带农业科学院热带生物技术研究所海口571101

基于线性回归分析、单因素方差分析、判别分析、主成分分析与聚类分析等方法对珠江水系、长江水系、淮河水系、辽河水系、海河水系、松花江水系19个自然群体共646个个体的外部可量性状进行形态学比较。线性回归分析结果显示,大鳞副泥鳅... 详细信息

基于线性回归分析、单因素方差分析、判别分析、主成分分析与聚类分析等方法对珠江水系、长江水系、淮河水系、辽河水系、海河水系、松花江水系19个自然群体共646个个体的外部可量性状进行形态学比较。线性回归分析结果显示,大鳞副泥鳅前驱长(DAC)/体长(LB)与纬度呈负相关(r=-0.782),尾柄长(LCP)/体长(LB)与纬度呈正相关(r=0.834)。单因素方差分析结果显示,松花江流域和辽河流域的形态学无显著差异,同时松辽流域与其他流域在全长(LB)、尾柄长(LCP)以及前驱长(DAC)表现出显著性差异。判别分析结果显示,松辽流域和南方水系的判别准确率为100%,两者之间没有错判。主成分分析结果显示,主成分1贡献率为38.1%,3个主成分可以解释大鳞副泥鳅不同地理群体形态差异的62.9%,这3个主成分主要反映在体高(HB)、体宽(WB)、尾柄高(HCP)、尾柄宽(LCP)。聚类分析结果显示,我国七大流域大鳞副泥鳅大致分为南北两大支。南方水系和北方水系大鳞副泥鳅在外部形态上存在一定程度差异,根据75%识别和划分规则,认为松辽流域和南方水系部分群体的大鳞副泥鳅形态学差异可能达到亚种水平。

关键词：大鳞副泥鳅地理群体形态差异种质资源种群结构比较分析聚类分析亲缘地理学

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团头鲂、鳡及其杂交F1代的形态差异与系统发育研究

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华中农业大学学报 2020年第1期39卷 116-125页

作者：陈静 SHALABY Hany said ali 易少奎王卫民华中农业大学水产学院/农业动物遗传育种与繁育教育部重点实验室/农业农村部淡水生物繁育重点实验室

利用不同亚科的雌性团头鲂(Megalobrama amblycephala)和雄性鳡(Elopichthys bambusa)进行人工杂交获得子一代杂交个体,对团头鲂、鳡及其杂交子代的形态特征进行比较和分析,并对团头鲂(♀)×鳡(♂)的线粒体全基因组序列进行扩增和分析... 详细信息

利用不同亚科的雌性团头鲂(Megalobrama amblycephala)和雄性鳡(Elopichthys bambusa)进行人工杂交获得子一代杂交个体,对团头鲂、鳡及其杂交子代的形态特征进行比较和分析,并对团头鲂(♀)×鳡(♂)的线粒体全基因组序列进行扩增和分析。结果显示,在传统可量性状和框架结构中,杂交F1的性状更加偏向于母本团头鲂。在可数性状上,杂交子代的背鳍棘数、胸鳍棘数、腹鳍棘数和臀鳍棘数与母本团头鲂及父本鳡都一致;而其余大多数性状,如背鳍鳍条数、腹鳍鳍条数、臀鳍鳍条数、侧线鳞数、侧线上鳞数、侧线下鳞数和鳔室数,杂交子代的性状更接近于母本团头鲂。杂交鱼的线粒体基因组序列全长为16623 bp,与母本团头鲂一致,其碱基组成为31.23%A、16.19%G、24.69%T和27.90%C,且具有一定的A+T偏向性。其线粒体基因组共编码37个基因,分别为13个蛋白质编码基因、22个tRNA基因和2个rRNA基因,还有1个非编码区(D-Loop)。系统发育树结果显示,团头鲂(♀)×鳡(♂)与母本团头鲂的亲缘关系较近,与父本鳡的亲缘关系较远。

关键词：团头鲂鳡远缘杂交亚科杂交形态差异系统发育线粒体基因组鲤科鱼类

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ZL114A大型铝铸件焊缝的组织与性能

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特种铸造及有色合金 2001年第5期21卷 14-15页

作者：李德成冯志军李玉胜赵芳欣尹绍奎沈阳铸造研究所

研究了ZL114A合金大型铸件熔化极气体保护焊 (MIG)焊补区域的组织与性能。金相观察表明 ,在靠近熔合线附近的热影响区内存在共晶复熔组织。焊补时 ,基体中Mg2 Si向复熔体扩散 ;远离熔合线的热影响区内 ,基体中固溶的Mg2 Si发生过时效 ,... 详细信息

研究了ZL114A合金大型铸件熔化极气体保护焊 (MIG)焊补区域的组织与性能。金相观察表明 ,在靠近熔合线附近的热影响区内存在共晶复熔组织。焊补时 ,基体中Mg2 Si向复熔体扩散 ;远离熔合线的热影响区内 ,基体中固溶的Mg2 Si发生过时效 ,从而导致基体软化 ,显微硬度下降。T1处理时不能消除复熔共晶和基体软化 ,故热影响区的宏观力学性能降低 ,但降幅不到 10 %。

关键词： ZL114A合金熔化极气体保护焊力学性能铝合金焊缝焊接铸件

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克氏原螯虾CSGalNAcT-1基因功能初步研究

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华中农业大学学报 2022年第4期41卷 233-239页

作者：欧阳号锋易少奎张龙杨思琦李艳和华中农业大学水产学院/农业农村部淡水动物育种重点实验室武汉430070 湖州师范学院生命科学学院湖州313000 教育部长江经济带大宗水生生物产业绿色发展工程研究中心武汉430070

为探究硫酸软骨素N-乙酰半乳糖胺基转移酶-1(chondroitin sulfate N-acetylgalactosaminyltransferase-1,CSGalNAcT-1)在克氏原螯虾免疫反应中的作用,利用RACE技术成功获得克氏原螯虾CSGalNAcT-1基因完整cDNA序列,并通过荧光定量PCR分析该基因在各组织中的表达模式以及在CpG寡脱氧核苷酸(ODN)、嗜水气单胞菌和白斑综合征病毒(WSSV)刺激后的表达情况。结果显示,克氏原螯虾CSGalNAcT-1基因在中肠的相对表达量最高,其次是近胃段肠,在心脏、胃、肌肉和围食道神经中的相对表达量较低。克氏原螯虾肝胰腺、血细胞中CSGalNAcT-1基因表达量在嗜水气单胞菌和WSSV刺激下呈现出下调及上调,而在注射免疫刺激剂CpG ODN后,CSGalNAcT-1基因表达水平在大部分组织中呈现上调。表明克氏原螯虾CSGalNAcT-1基因可能在抵御外界病原侵染以及增强自身免疫反应的过程中发挥重要作用。

关键词：克氏原螯虾 CSGalNAcT-1基因相对表达白斑综合征病毒(WSSV) CpG ODN 嗜水气单胞菌

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新疆塔里木河叶尔羌高原鳅群体遗传学研究

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水产科学 2023年第4期42卷 664-673页

作者：赵文浩易少奎周琼沈建忠李大鹏周小云华中农业大学水产学院农业农村部淡水生物繁育重点实验室湖北武汉430070 湖州师范学院生命科学学院浙江湖州313000

为探明新疆塔里木河流域叶尔羌高原鳅群体遗传学特征,基于线粒体COⅠ基因和微卫星标记对塔里木河两条支流(车尔臣河和渭干河)的8个叶尔羌高原鳅群体进行遗传多样性和遗传结构分析。结果显示,基于COⅠ序列共检测到22个单倍型,平均单倍型... 详细信息

为探明新疆塔里木河流域叶尔羌高原鳅群体遗传学特征,基于线粒体COⅠ基因和微卫星标记对塔里木河两条支流(车尔臣河和渭干河)的8个叶尔羌高原鳅群体进行遗传多样性和遗传结构分析。结果显示,基于COⅠ序列共检测到22个单倍型,平均单倍型多样性指数和核苷酸多样性指数分别为0.8829和0.0032。基于新开发的10个微卫星标记分析显示,各群体的多态信息含量、观测杂合度和期望杂合度分别为0.629~0.696、0.607~0.736和0.655~0.755。试验结果表明,各群体的遗传多样性水平均较高。两种标记分析结果均显示,群体间遗传分化指数较低;分子方差分析显示,遗传变异主要来源于群体内部(COⅠ, 95.12%;微卫星,97.23%)。群体间遗传距离小,群体间基因流均大于1。试验结果表明,群体间的遗传分化程度较弱,存在一定的基因交流。基于微卫星标记构建的非加权组平均法系统发育树显示,渭干河群体和车尔臣河群体位于不同的遗传分支;群体结构分析显示,两支流群体的基因组成有明显差异;Barrier分析显示,两支流群体间存在潜在遗传障碍;研究结果表明,两支流群体间存在明显的遗传分化。综上所述,塔里木河叶尔羌高原鳅各群体遗传多样性水平较高,群体间有一定的基因交流,但由于塔里木河生境片段化的问题,导致两支流群体之间存在潜在遗传障碍,并出现了一定的遗传分化。因此,有必要采取措施对其栖息环境进行保护,避免种群遗传多样性的降低。

关键词：叶尔羌高原鳅塔里木河遗传多样性遗传结构

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