检索结果-南通市图书馆

Photonics Research 2023年第3期11卷 442-455页

作者： YAO FAN JIASONG SUN yefeng shu ZEYU ZHANG QIAN CHEN CHAO ZUO Smart Computational Imaging(SCI)Laboratory Nanjing University of Science and TechnologyNanjing 210094China Smart Computational Imaging Research Institute(SCIRI)of Nanjing University of Science and Technology Nanjing 210019China Jiangsu Key Laboratory of Spectral Imaging&Intelligent Sense Nanjing University of Science and TechnologyNanjing 210094China

Quantitative phase imaging(QPI)by differential phase contrast(DPC)with partially coherent illumination provides speckle-free imaging and lateral resolution beyond the coherent diffraction limit,demonstrating great potential in biomedical imaging ***,DPC employs weak object approximation to linearize the phase-to-intensity image formation,simplifying the solution to the phase retrieval as a two-dimensional deconvolution with the corresponding phase transfer *** its widespread adoption,weak object approximation still lacks a precise and clear definition,suggesting that the accuracy of the QPI results,especially for samples with large phase values,is yet to be *** this paper,we analyze the weak object approximation condition quantitatively and explicitly give its strict definition that is applicable to arbitrary samples and illumination ***,an iterative deconvolution QPI technique based on pseudo-weak object approximation is proposed to overcome the difficulty of applying DPC to large-phase samples without additional data *** with standard microlens arrays and MCF-7 cells demonstrated that the proposed method can effectively extend DPC beyond weak object approximation to high-precision three-dimensional morphological characterization of large-phase technical and biological samples.

关键词： approximation illumination coherent

Adaptive optical quantitative phase imaging based on annular illumination Fourier ptychographic microscopy

在线全文

学校读者我要写书评

暂无评论

PhotoniX 2022年第1期3卷 101-115页

作者： yefeng shu Jiasong Sun Jiaming Lyu Yao Fan Ning Zhou Ran Ye Guoan Zheng Qian Chen Chao Zuo Smart Computational Imaging Laboratory(SCILab) School of Electronic and Optical EngineeringNanjing University of Science and Technolog210094 NanjingJiangsu ProvincePeople’s Republic of China Smart Computational Imaging Research Institute(SCIRI)of Nanjing University of Science and Technology 210019 NanjingJiangsu ProvincePeople’s Republic of China Jiangsu Key Laboratory of Spectral Imaging Intelligent Sense 210094 NanjingJiangsu ProvincePeople’s Republic of China Terahertz Technology Innovation Research Institute University of Shanghai for Science and Technology200093 ShanghaiPeople’s Republic of China School of Computer and Electronic Information Nanjing Normal University210023 NanjingJiangsu ProvincePeople’s Republic of China Department of Biomedical Engineering University of Connecticut06269 StorrsConnecticutUSA

Quantitative phase imaging(QPI)has emerged as a valuable tool for biomedical research thanks to its unique capabilities for quantifying optical thickness variation of living cells and *** many QPI methods,Fourier ptychographic microscopy(FPM)allows long-term label-free observation and quantitative analysis of large cell populations without compromising spatial and temporal ***,high spatio-temporal resolution imaging over a long-time scale(from hours to days)remains a critical challenge:optically inhomogeneous structure of biological specimens as well as mechanical perturbations and thermal fluctuations of the microscope body all result in time-varying aberration and focus drifts,significantly degrading the imaging performance for long-term ***,the aberrations are sample-and environmentdependent,and cannot be compensated by a fixed optical design,thus necessitating rapid dynamic correction in the imaging ***,we report an adaptive optical QPI method based on annular illumination *** this method,the annular matched illumination configuration(i.e.,the illumination numerical aperture(NA)strictly equals to the objective NA),which is the key for recovering low-frequency phase information,is further utilized for the accurate imaging aberration *** using only 6 low-resolution images captured with 6 different illumination angles matching the NA of a 10x,0.4 NA objective,we recover high-resolution quantitative phase images(synthetic NA of 0.8)and characterize the aberrations in real time,restoring the optimum resolution of the system *** our method to live-cell imaging,we achieve diffraction-limited performance(full-pitch resolution of 655 nm at a wavelength of 525 nm)across a wide field of view(1.77mm2)over an extended period of time.

关键词： Quantitative phase imaging Fourier ptychographic microscopy Adaptive optics Long-term imaging

Hybrid brightfield and darkfield transport of intensity approach for high-throughput quantitative phase microscopy

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advanced Photonics 2022年第5期4卷 15-26页

作者： Linpeng Lu Jiaji Li yefeng shu Jiasong Sun Jie Zhou Edmund Y.Lam Qian Chen Chao Zuo Nanjing University of Science and Technology Smart Computational Imaging Laboratory(SCILab)NanjingChina Nanjing University of Science and Technology School of Electronic and Optical EngineeringNanjingChina Jiangsu Key Laboratory of Spectral Imaging&Intelligent Sense NanjingChina Nanjing University of Science and Technology Smart Computational Imaging Research Institute(SCIRI)NanjingChina The University of Hong Kong Department of Electrical and Electronic EngineeringPokfulamHong KongChina

Transport of intensity equation(TIE)is a well-established non-interferometric phase retrieval approach that enables quantitative phase imaging(QPI)by simply measuring intensity images at multiple axially displaced *** advantage of a TIE-based QPI system is its compatibility with partially coherent illumination,which provides speckle-free imaging with resolution beyond the coherent diffraction ***,TIE is generally implemented with a brightfield(BF)configuration,and the maximum achievable imaging resolution is still limited to the incoherent diffraction limit(twice the coherent diffraction limit).It is desirable that TIE-related approaches can surpass this limit and achieve high-throughput[high-resolution and wide field of view(FOV)]*** propose a hybrid BF and darkfield transport of intensity(HBDTI)approach for highthroughput quantitative phase *** through-focus intensity stacks corresponding to BF and darkfield illuminations are acquired through a low-numerical-aperture(NA)objective *** high-resolution and large-FOV complex amplitude(both quantitative absorption and phase distributions)can then be synthesized based on an iterative phase retrieval algorithm taking the coherence model decomposition into *** effectiveness of the proposed method is experimentally verified by the retrieval of the USAF resolution target and different types of biological *** experimental results demonstrate that the half-width imaging resolution can be improved from 1230 nm to 488 nm with 2.5×expansion across a 4×FOV of 7.19 mm2,corresponding to a 6.25×increase in space-bandwidth product from∼5 to∼30.2 *** contrast to conventional TIE-based QPI methods where only BF illumination is used,the synthetic aperture process of HBDTI further incorporates darkfield illuminations to expand the accessible object frequency,thereby significantly extending the maximum available resolution from 2NA to∼5NA with a∼5×promotion of the coherent diffra

关键词： transport of intensity equation phase retrieval darkfield imaging high-throughput microscopy

Correction:Adaptive optical quantitative phase imaging based on annular illumination Fourier ptychographic microscopy

在线全文

学校读者我要写书评

暂无评论

PhotoniX 2022年第1期3卷 73-73页

In the original publication of this article[1],the video in the additional file 2 was uploaded mistakenly due to a typesetting error,and needs to be updated with the correct *** original article[1]was updated.

关键词： illumination annular phase

大鼠纹状体边缘区的多巴胺受体D1A参与了学习记忆功能

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

神经解剖学杂志 2012年第1期28卷 23-27页

作者：周莹蔡业峰李东风肖鹏舒斯云华南师范大学生命科学学院广州510631 广东省中医院第一神经内科内科广州510120 华南师范大学认知与神经科学研究所广州510631

目的:研究多巴胺受体D1A在纹状体边缘区的分布及其是否参与了大鼠的学习记忆过程。方法:先用免疫组织化学方法观察多巴胺受体D1A在纹状体边缘区的分布,再向纹状体边缘区内立体定位微量注射多巴胺受体D1A拮抗剂SCH-23390(1%,0.1μl/侧)... 详细信息

目的:研究多巴胺受体D1A在纹状体边缘区的分布及其是否参与了大鼠的学习记忆过程。方法:先用免疫组织化学方法观察多巴胺受体D1A在纹状体边缘区的分布,再向纹状体边缘区内立体定位微量注射多巴胺受体D1A拮抗剂SCH-23390(1%,0.1μl/侧)或等量的生理盐水,检测大鼠注射前后Y-迷宫结果的变化。结果:多巴胺受体D1A免疫组化阳性产物广泛分布于大脑皮层、海马、杏仁核、Meynert基核、下丘脑等处的胞体和突起上。在纹状体内,多巴胺受体D1A的阳性产物广泛而不均匀地分布于神经元的细胞核内,密度最大的区域在纹状体的尾内侧边缘。在纹状体尾内侧边缘,分布有多层密集平行排列的多巴胺受体D1A强阳性的椭圆形胞核,围绕苍白球外侧边缘,沿背腹方向呈带状分布,细胞核形态与位置和边缘区一致。行为实验证明,边缘区微量注射多巴胺受体D1A拮抗剂SCH-23390后,显著降低了大鼠的长期学习记忆能力。结论:大鼠脑纹状体边缘区神经元内存在多巴胺受体D1A,它们参与了成年SD大鼠的长期学习记忆功能。

关键词：多巴胺受体纹状体大鼠脑免疫组化 Y-迷宫

纯电动汽车CO_(2)热泵模块与热管理系统方案设计

在线全文

学校读者我要写书评

暂无评论

汽车工程师 2021年第4期 33-35页

作者：唐丹萍王子安王雨晴刘业凤何曙上海市质量监督检验技术研究院上海仪器仪表自控系统检验测试所有限公司上海理工大学能源与动力工程学院

随着环境和能源问题的加剧,纯电动汽车作为无污染、安全可靠的交通工具渐渐进入汽车市场。由于燃油汽车的空调系统不适配,且面临传统制冷剂淘汰的风险,因此文章综合考虑功能、性能、可靠性等因素设计了1套CO_(2)热泵模块与热管理系统,... 详细信息

随着环境和能源问题的加剧,纯电动汽车作为无污染、安全可靠的交通工具渐渐进入汽车市场。由于燃油汽车的空调系统不适配,且面临传统制冷剂淘汰的风险,因此文章综合考虑功能、性能、可靠性等因素设计了1套CO_(2)热泵模块与热管理系统,该系统特点在于通过载冷剂回路来实现冷热量传递,并增加余热回收功能以降低热泵模块耗电量。

关键词：纯电动汽车 CO_(2)热泵模块热管理系统余热回收

Dysbindin-1与脆性X相关蛋白1、Y盒结合蛋白2的相互作用

在线全文

学校读者我要写书评

暂无评论

生命的化学 2021年第12期41卷 2667-2675页

作者：李海雁张姝张盼盼孙欣袁业锋冯雅琴山西医科大学组织胚胎学教研室太原030001 首都医科大学附属北京儿童医院北京100045

(肌)营养不良短小蛋白结合蛋白1(dystrobrevin-binding protein 1, dysbindin-1)是溶酶体相关细胞器生物发生复合体-1(biogenesis of lysosome-related organelles complex-1, BLOC-1)的一个亚基,在内体-溶酶体通路的货物运输中发挥一定作用。Dysbindin-1在睾丸组织中的作用尚不明确。本文从前期已筛选到的dysbindin-1在小鼠睾丸组织中的潜在互作蛋白质中,选取2个RNA结合蛋白(RNA-binding proteins, RBPs)脆性X相关蛋白1(fragile X related protein 1, FXR1P)和Y盒结合蛋白2(Y-box binding protein 2, YBX2),用GST pull-down和内源性免疫沉淀的方法进行相互作用的验证。结果显示,dysbindin-1与FXR1P和YBX2存在相互作用,提示dysbindin-1在睾丸组织RNA的运输中可能发挥作用。

关键词： dysbindin-1 FXR1P YBX2 相互作用小鼠睾丸

同方期刊数据库