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An approach to extracting surface supply relationships between glaciers and lakes on the Tibetan Plateau

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International Journal of Digital Earth 2018年第11期11卷 1151-1165页

作者： Bei-Bei Ai Cheng-Zhi Qin qinghua ye A-Xing Zhu Graham Cogley State Key Laboratory of Resources and Environmental Information System Institute of Geographic Sciences and Natural Resources ResearchCASBeijingPeople’s Republic of China College of Resources and Environment University of Chinese Academy of SciencesBeijingPeople’s Republic of China Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application and School of Geography Nanjing Normal UniversityNanjingPeople’s Republic of China Key Laboratory of Tibetan Environment Changes and Land Surface Processes Institute of Tibetan Plateau ResearchCASBeijingPeople’s Republic of China Department of Geography University of Wisconsin-MadisonMadisonWIUSA Key Laboratory of Virtual Geographic Environment Ministry of EducationNanjing Normal UniversityNanjingPeople’s Republic of China Department of Geography Trent University PeterboroughCanada

Surface supply relationships between glaciers and lakes are needed to analyze and understand hydrological processes at regional and global ***,these supply relationships still cannot be extracted efficiently by existing *** paper proposes an automatic and efficient approach to extracting surface supply relationships between glaciers and lakes based on meltwater flow *** approach includes two stages:(1)identifying direct connections between objects(*** and lakes)based on flow direction derived from digital terrain analysis on a gridded digital elevation model(DEM)and(2)deriving all(or user-specified)kinds of surface supply relationships based on graph *** computation-intensive steps in this approach have been parallelized;and all steps in the proposed approach have been integrated as an automatic *** for the Tibetan Plateau show that given outline data for glaciers and lakes and the Shuttle Radar Topography Mission DEM,the proposed approach can automatically derive diverse surface supply relationships under userspecified restrictions on the attributes of the supply *** parallelization in the approach effectively improves the computing *** proposed approach could also be applied to developing a detailed fundamental dataset of supply relationships between glaciers and lakes for other region or period.

关键词： Glaciers and lakes surface supply relationship digital terrain analysis parallel computing Tibetan Plateau

基于免疫蛋白质组学的单增李斯特菌强免疫原性蛋白挖掘

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现代食品科技 2024年第3期40卷 301-308页

作者：张颖吴诗古其会叶青华张菊梅陈谋通吴清平华南农业大学食品学院广东广州510642 广东省科学院微生物研究所华南应用微生物国家重点实验室广东省微生物安全与健康重点实验室农业农村部农业微生物组学与精准应用重点实验室广东广州510070

该研究采用高毒力持留基因型单增李斯特菌819-2菌株全菌蛋白免疫SPF级Balb/C小鼠制备抗血清,利用免疫蛋白质组学对菌株胞外蛋白质组进行分析,旨在挖掘筛选单增李斯特菌强免疫原性蛋白作为特异性抗体制备的候选抗原。超声法提取819-2菌... 详细信息

该研究采用高毒力持留基因型单增李斯特菌819-2菌株全菌蛋白免疫SPF级Balb/C小鼠制备抗血清,利用免疫蛋白质组学对菌株胞外蛋白质组进行分析,旨在挖掘筛选单增李斯特菌强免疫原性蛋白作为特异性抗体制备的候选抗原。超声法提取819-2菌株全菌蛋白免疫SPF级Balb/C小鼠制备抗血清,四次免疫后经间接ELISA测定效价达1:512000。脱氧胆酸钠(DOC)-10%(m/V)TCA沉淀法提取单增李斯特菌819-2菌株胞外蛋白,利用免疫蛋白质组学和LC-MS/MS技术挖掘并鉴定具有强免疫反应的蛋白点,结果表明双向电泳图谱成功获得85个蛋白点,并成功鉴定了P60、InlC、MltG、Enolase和假定蛋白YxeA family protein等5个强免疫原性蛋白,研究结果为基于强免疫原性蛋白制备特异性抗体用于食品及其加工环境中单增李斯特菌富集及快速检测技术研制提供了数据基础。

关键词：单增李斯特菌免疫蛋白质组学抗血清免疫印迹

基于数值鉴定法的肠杆菌科生化试剂盒的研制及效果评价

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微生物学报 2024年第5期64卷 1668-1682页

作者：金乐萱万强曹璐璐叶青华王迅张菊梅蔡芷荷吴清平合肥工业大学食品与生物工程学院安徽合肥230601 广东省科学院微生物研究所、华南应用微生物国家重点实验室、广东省微生物安全与健康重点实验室国家卫健委微生物食品营养与安全科技创新平台广东广州510070 广东环凯生物科技有限公司广东肇庆526238

【背景】肠杆菌科细菌是食源性和临床感染的主要病原菌,对人类和动物健康有重要影响,以API 20E为“金标准”的数值鉴定法是其主要的鉴定方法之一,但现有的数值鉴定方法存在手工单个加样或/和准确率低、价格贵的问题。【目的】研制出半... 详细信息

【背景】肠杆菌科细菌是食源性和临床感染的主要病原菌,对人类和动物健康有重要影响,以API 20E为“金标准”的数值鉴定法是其主要的鉴定方法之一,但现有的数值鉴定方法存在手工单个加样或/和准确率低、价格贵的问题。【目的】研制出半自动化、准确性高及价格低的肠杆菌科细菌数值鉴定生化试剂盒。【方法】在本团队前期建立的肠杆菌科数值鉴定系统理论模型与配套软件的基础上,设计并优化筛选出的24种生化基质微量化配方,在此基础上,研制半自动冻干鉴定条;以现行商品化的数值鉴定条API 20E、MALDI-TOF MS及16S rRNA基因测序为对照,对研制的集半自动生化鉴定条与在线分析软件于一体的生化试剂盒进行效果评价。【结果】共获得458株肠杆菌科细菌的生化谱,在属水平总体鉴定准确率达到98.5%,在种水平总体鉴定准确率达到96.5%;仅需两次加样即可获得鉴定结果,且价格仅为API 20E的4.46%;产品保质期为7 d,生化试验具有稳定可重复性。【结论】本研究研制出的半自动化肠杆菌科细菌数值鉴定生化试剂盒简便经济、准确率高,为肠杆菌科细菌的鉴定提供技术支撑,并为其他科属细菌的数值鉴定生化产品的开发提供技术指引。

关键词：肠杆菌科数值鉴定生化试剂盒效果评价

Geospatial-temporal analysis of land-use changes in the yellow River Delta during the last 40 years

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Science China Earth Sciences 2004年第11期47卷 1008-1024页

作者： ye qinghua LIU Gaohuan TIAN Guoliang CHEN Shenliang HUANG Chong CHEN Shupeng LIU Qingshen CHANG Jun SHI Yanan State Key Laboratory of Remote Sensing Science Institute of Remote Sensing Applications(IRSA)Chinese Academy of Science(CAS)Beijing 100101China Institute of Geographic Sciences and Natural Resources Research CASBeijing 100101China State Key Laboratory of Estuarine and Coastal Research East China Normal UniversityShanghai 200062China Institute of Tibetan Plateau Research(ITPR) CASBeijing 100085China

Comprehensive study on land-use change of spatial pattern and temporal process is the key component in land use/land cover changes (LUCC) study nowadays. According to the theories and methods of Geo-information Tupu (Carto-methodology in Geo-information, CMGI) for geospatial and temporal analysis and integration models of spatial pattern and temporal processes in GIS, the paper puts forward Tupu methodology of land-use change and names the basic synthetic unit of spatial-temporal information Tupu unit. Tupu unit is the synthetic unit for geospatial-temporal analysis, which is synthesized by “space·attribute·process”features and composed of relatively homogeneous geographic unit and temporal unit. Based on the spatial features of 4 stages of land-use change in 1956, 1984, 1991, and 1996, a series of Tupu on land-use change in the yellow River Delta (YRD) are created and studied in the paper, which includes 3 temporal units of spatial-temporal Tupu, process Tupu of land-use changes during the last 40 years, and reconstructions of a series of “arisen”Tupu, “arisen”process Tupu and pattern Tupu on land-use changes in the last 40 years by remapping tables of a reclassifying system. There are 3 methods of Tupu analysis on land-use change that are used to disclose change processes of land-use spatial conversion in YRD, such as spatial query and statistics, order of Tupu units by area and land-use conversion matrixes. In order to reveal the spatial pattern of land-use change processes, we analyze spatial-temporal changes of each Tupu above in various temporal units and spatial difference of pattern Tupu in the last 40 years by dynamic Tupu units. Tupu analysis on regional land-use is a promising trial on the comprehensive research of “spatial pattern of dynamic process”and “temporal process of spatial pattern”in LUCC research. The geo-information Tupu methodology would be a powerful and efficient tool on integrated studies of spatial pattern and temporal process in geoscien

关键词： eo-information Tupu spatial-temporal analysis the yellow River Delta land-use changes Tupu Unit

Shifts in water-level variation of Namco in the central Tibetan Plateau from ICESat and Cryo Sat-2 altimetry and station observations

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Science Bulletin 2015年第21期60卷 1287-1297页

作者： Chunqiao Song qinghua ye Xiao Cheng Institute of Tibetan Plateau Research (ITP) Chinese Academy of Sciences Department of Geography University of California State Key Laboratory of Remote Sensing Science College of Global Change and Earth System Science Beijing Normal University

The dynamics of high-altitude inland lakes in the Tibetan Plateau are sensitive indicators of climate change. Due to the remoteness and hard access, satellite altimetry becomes an effective approach to obtaining largescale and temporally continuous information of lake-level changes. The CryoSat-2 altimetry is expected to solve the current problem that earlier radar altimeters are only practical for monitoring large water bodies, while ICESat laser altimetry is available only for the period 2003-2009. In this study, the comparison of CryoSat-2 altimetry for Namco with in situ water-level data suggests a high cor- relation coefficient of 0.71 （P 〈 0.01）, with the mean error of -0.12 m and root-mean-square error of 0.18 m. Further, the combination of ICESat and CryoSat-2 altimetry data and in situ lake-level observations reveals a rapid water- level rise of 0.24 4- 0.04 m/year during 2003-2008 and then a slightly decreasing trend of -0.09 4- 0.04 m/year during 2009-2013. This study suggests that the CryoSat-2 altimetry has the potential of sustaining the fine observa- tions on Tibetan lakes, following the ICESat mission. Besides, the examination of four key climatic variables （temperature, precipitation, potential evapotranspiration, and relative humidity） during 1990-2013 indicates that the wetting climate over Namco Basin stagnated or even reversed around 2006, which may be tightly related to the slowing lake growth.

关键词： Lake Satellite altimetry CryoSat-2ICESat Tibetan Plateau - Climate change

克罗诺杆菌属细菌数值鉴定系统的研制与效果评价

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微生物学通报 2024年第8期51卷 3165-3178页

作者：曹璐璐万强金乐萱吴毓薇赵昕宇高宝曲晓莹蒋富凤叶青华吴清平广东环凯生物科技有限公司广东肇庆526238 广东省科学院微生物研究所华南应用微生物国家重点实验室广东省微生物安全与健康重点实验室国家卫健委微生物食品营养与安全科技创新平台广东广州510070 合肥工业大学食品与生物工程学院安徽合肥230009

【背景】克罗诺杆菌属(Cronobacter)细菌的分离鉴定是临床医学中精准治疗的理论基础,然而,目前的生化鉴定方法仅可将其鉴定至属水平。【目的】开发一种简便、可靠的克罗诺杆菌属生化分种鉴定方法,建立克罗诺杆菌属细菌数值鉴定系统并对... 详细信息

【背景】克罗诺杆菌属(Cronobacter)细菌的分离鉴定是临床医学中精准治疗的理论基础,然而,目前的生化鉴定方法仅可将其鉴定至属水平。【目的】开发一种简便、可靠的克罗诺杆菌属生化分种鉴定方法,建立克罗诺杆菌属细菌数值鉴定系统并对其鉴定性能进行评价。【方法】将鉴定系统所需的生化反应配方浓缩整合到一组注塑件中制得鉴定条,辅以数值鉴定理论构建克罗诺杆菌属细菌数值鉴定系统,利用克罗诺杆菌属标准株及分离株对其鉴定准确度进行验证。分离株的鉴定以fusA基因序列分析方法的鉴定结果作为参考标准,与数值鉴定法的检测结果进行比较。【结果】创建了用于鉴别克罗诺杆菌属内9个种/亚种的生化反应阳性概率数据库,同时确定了数值法中的计算方法和结果评价标准,并完成了数值鉴定系统软件的开发。5株克罗诺杆菌属标准株通过细菌数值鉴定系统均得到了正确鉴定,结果评价为极好的鉴定结果;其中,DSM 18707被准确鉴定为都柏林克罗诺杆菌奶粉亚种(*** ***)。测试集的84株克罗诺杆菌属分离株的数值鉴定法与fusA基因测序法的总鉴定一致率为100%;除*** IV型(1株)及*** III型(1株)的数值鉴定为不可接受的鉴定结果外,其余菌株均得到了明确的鉴定。【结论】本研究开发的克罗诺杆菌属细菌数值鉴定系统操作简便、价格低廉、准确率高,可为临床诊断及相关食品安全检测提供实验依据,具有良好的应用推广前景。

关键词：克罗诺杆菌属生化鉴定数值鉴定系统微生物安全

A broadband high resolution direction of arrival estimation algorithm based on conditional wavenumber spectral density

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Chinese Journal of Acoustics 2020年第4期39卷 482-497页

作者： LI Xuemin HUANG Haining LI Yu ye qinghua ZHANG Yangfan Institute of Acoustics Chinese Academy of SciencesBeijing 100190 Key Laboratory of Aduanced Underuwater Information Chinese Academy of SciencesBeijing 100190 University of Chinese Academg of Sciences Beijing 100049

A broadband high resolution direction of arrival(DOA) estimation algorithm named the conditional wavenumber spectral density based(CWSD-based) is proposed in this paper aimed at the problem of large error and high complexity of wideband high resolution *** super-resolution DOA estimation,minimal main-lobe width,ultra-low side-lobe and high precision target resolution performance can be obtained after transforming the array signals to f-k space and using the characteristic of wideband signal power distributed as a line on the conditional wavenumber spectral ***,there are no DOA pre-estimates of the incident signals and the number of sources to be required in this *** simulations indicate that the theoretical resolution of the algorithm is inversely proportional to the highest processing frequency,the estimated mean square error is about 0.1°,and also is robust to array shape distortion and has high computing *** data on the sea indicates that the proposed algorithm,whose performance is superior to CBF and MVDR in the aspects of resolution,weak target detection,non-target direction noise suppression and robustness et al.,can achieve ultra-low sidelobe high resolution DOA in the actual ocean.

关键词： resolution estimation algorithm

Spatial-temporal characteristics in landscape evolution of the yellow River Delta during 1855- 2000 and a way out for the yellow River estuary

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Chinese Science Bulletin 2006年第B11期51卷 197-209页

作者： ye qinghua CHEN Shenliang CHEN Qi HUANG Chong TIAN Guoliang CHEN Shupeng SHI Yanan LIU Qingshen LIU Gaohuan State Key Laboratory of Remote Sensing Science Institute of Remote Sensing Applications (IRSA) Chinese Academy of Sciences Beijing 100101 China State Key Laboratory of Estuarine and Coastal Research East China Normal University Shanghai 200062 China Institute of Geographical Sciences and Natural Resources Research Chinese Academy of Sciences Beijing 100101 China Institute of Tibetan Plateau Research Chinese Academy of Sciences Beijing 100085 China Department of Environmental Science Policy and ManagementUniversity of California Berkeley Berkeley 94720 USA

基于风景生态学 andGee 信息 Tupu 的图形、量的方法论，这篇论文学习风景黄河三角洲(YRD ) 的空间时间的进化特征，它被黄河(年) 尾部隧道的周期的物理秋千在 1855-2000 期间建立。根据关于 YRDand 年尾部隧道的空间时间的进化的研究... 详细信息

基于风景生态学 andGee 信息 Tupu 的图形、量的方法论，这篇论文学习风景黄河三角洲(YRD ) 的空间时间的进化特征，它被黄河(年) 尾部隧道的周期的物理秋千在 1855-2000 期间建立。根据关于 YRDand 年尾部隧道的空间时间的进化的研究，我们断定 Qingshuigou 隧道到达了它的限制，一条新流动隧道迟早多年应该被建立。它建议年隧道的移动应该接受它在以前的隧道之中通过消沉流动的自然规则并且能被人造的努力执行。论文在河口在 YR 流动为淤泥的高内容的处理外面讨论这些问题和交替的方法。

关键词：黄河三角洲黄河河口景观物理演化进程水文学

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上海复合极端风暴洪水淹没模拟

水科学进展 2019年第4期30卷 546-555页

作者：王璐阳张敏温家洪种振涛 ye qinghua KE Qian 上海师范大学环境与地理科学学院上海200234 华东师范大学河口海岸学国家重点实验室上海200062 Deltares Boussinesqweg 12629 HV Delftthe Netherlands Department of Hydraulic Engineering Delft University of TechnologyStevinweg 12628 CN Delftthe Netherlands

河口三角洲和沿海城市面临着台风、暴雨、高潮位和上游下泄洪水叠加的“四碰头”复合极端风暴洪水的严重威胁。构建了大气-海洋-陆地相耦合的一体化数值模拟系统,实现了上海市“风”“暴”“潮”“洪”多灾种复合情景的极端洪涝淹没模拟... 详细信息

河口三角洲和沿海城市面临着台风、暴雨、高潮位和上游下泄洪水叠加的“四碰头”复合极端风暴洪水的严重威胁。构建了大气-海洋-陆地相耦合的一体化数值模拟系统,实现了上海市“风”“暴”“潮”“洪”多灾种复合情景的极端洪涝淹没模拟,并验证了耦合方法的有效性,为复合风暴洪水的一体化模拟提供了一套可行的数值模拟方法。在9711台风影响下,模拟了1998年堤防升级改造后淹没面积(水深>0.2m)比改造前减少了62%,表明沿海沿江堤防设施建设在上海市防台防汛中起着关键性的作用。复合极端风暴洪水的有效模拟可为财产保险和未来市政规划提供参考。

关键词：复合风暴洪水数值模拟模型耦合上海