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Journal of Environmental Sciences 2006年第2期18卷 373-378页

作者： ye yang-fang MIN Hang LU Zhen-mei College of Life Science and Biotechnology Ningbo University Ningbo 315211 China College of Life Science Zhejiang University Hangzhou 310029 China

The effect of acetanilide herbicide mefenacet on soil microbial communities was studied using paddy soil samples with different short-term treatments. The culturable bacteria （plate counts）, dehydrogenase activity and changes in community structure （denaturing gradient gel electrophoresis （DGGE） analysis） were used for biological community assessments. Mefenacet was a significant stimulus to cultural aerobic bacteria and dehydrogenase activity while Sphingobacterium multivorum Y1, a bacterium efficiently degrading the mefenacet, only induced the increasing colony-forming unit （CFU） of bacteria but little effect on dehydrogenase activity during the whole experiment. The degree of similarity between the 16S rDNA profiles of the communities was quantified by numerically analyzing the DGGE band patterns. Similarity dendrograms showed that the microbial community structures of the mefenacet-treated and non-treated soils were not significantly different. But supplement of S. multivorum Y1 could increase the diversity of the microbial community in the mefenacet-polluted paddy soil. This work is a new attempt to apply the S. multivorum Y1 for remediation of the mefenacet-polluted environments.

关键词： denaturing gradient gel electrophoresis(DGGE) soil microbial diversity mefenacet dehydrogenase

GPC3 fused to an alpha epitope of HBsAg acts as an immune target against hepatocellular carcinoma associated with hepatitis B virus

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Hepatobiliary & Pancreatic Diseases International 2011年第2期10卷 164-170页

作者： Jun-Wen yang,Dong-ye yang,fang-Gen Lu,Cai-Hong Li,Hui Chen,Ning Xie and Xin Zhao Department of Digestive Diseases,Second Xiangya Hospital,Central South University,Changsha 410011,China Department of Digestive Diseases Second Xiangya Hospital Central South University Changsha 410011 China

BACKGROUND:The incidence of hepatocellular carcinoma (HCC)in China is closely related to the population infected with hepatitis B virus(HBV).HCC cells with HBV secrete soluble HBsAg into blood but do not express it on the cell membrane This study aimed to construct and investigate a new glycosyl phosphatidylinositol(GPI)-anchored protein(GPC3+α+EGFP) as a DNA vaccine against HCC associated with HBV. METHODS:A recombinant plasmid(pcDNA3.1(+)/GPC3+ α+EGFP)was constructed and verified by restriction endo nuclease digestion and ***3.1(+)/GPC3+α+ EGFP was transfected into HepG2 cells(experimental group) using lipofectamine ***-N1-transfected HepG2 cells were used as a negative control,and non-transfected HepG2 cells sreved as a blank ***2 cells that steadily expressed the fusion protein GPC3+α+EGFP were screened by G418,propagated,and co-cultured with lymphocytes from healthy *** proliferation was measured by the classic sulforhodamine B *** was assessed by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL),and Fas gene transcription was determined by quantitative fluorescent PCR. RESULTS:The pcDNA3.1(+)/GPC3+α+EGFP plasmid was successfully *** the experimental group,green fluorescence was observed at the cell periphery and in the cytoplasm,whereas in the negative control group,fluorescence was evenly distributed throughout the *** of the experimental group significantly decreased after 72 hours compared to the negative and blank control ***,the number of apoptotic cells was statistically different among the three groups as determined by a contingency table Chisquare test;the experimental group had the highest incidence of *** gene transcription in the experimental group was higher than in the two control groups,and an increasing trend with time in the experimental group was observed. CONCLUSION:A chimeric,membrane-anchored protein, GPC3+α+EGFP,localized

关键词： HBsAg-αepitope glypican 3 hepatocellular carcinoma hepatitis B virus protein engineering

基于核磁共振的代谢组学技术在食品科学中的应用

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食品与发酵工业 2016年第3期42卷 256-261页

作者：陈黛安叶央芳宁波大学海洋学院浙江宁波315211

基于核磁共振(nuclear magnetic resonance,NMR)的代谢组学技术是组学研究中一个正在蓬勃兴起的技术,该技术专注于生物体中所有小分子代谢物的高通量分析,但在对食品的物质组成分析上也同样行之有效。该文就近年来该技术在食品科学领域... 详细信息

基于核磁共振(nuclear magnetic resonance,NMR)的代谢组学技术是组学研究中一个正在蓬勃兴起的技术,该技术专注于生物体中所有小分子代谢物的高通量分析,但在对食品的物质组成分析上也同样行之有效。该文就近年来该技术在食品科学领域的最新研究进展做一简要综述。

关键词：核磁共振(nuclear magnetic resonance,NMR) 代谢组学食品科学

基于多达理念的延续护理干预对脑卒中患者吞咽和行为功能康复的影响

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护理实践与研究 2019年第19期16卷 54-56页

作者：杨叶芳无锡市第二人民医院A9病房

目的探讨基于多达理念的延续护理干预对脑卒中患者吞咽和行为功能康复的影响效果。方法选取2017年1月至2019年1月我院收治的93例脑卒中患者为研究对象,随机将其分为研究组47例和对照组46例,对照组实施常规护理,研究组实施基于多达理念... 详细信息

目的探讨基于多达理念的延续护理干预对脑卒中患者吞咽和行为功能康复的影响效果。方法选取2017年1月至2019年1月我院收治的93例脑卒中患者为研究对象,随机将其分为研究组47例和对照组46例,对照组实施常规护理,研究组实施基于多达理念的延续护理干预,比较两组脑卒中患者吞咽和行为功能康复效果。结果研究组患者吞咽功能恢复效果高于对照组(P<0.05);研究组患者FMI和MAI功能评分高于对照组(P<0.05)。结论脑卒中患者应用基于多达理念的延续护理,可有效刺激吞咽功能障碍患者神经反馈能力,进而逐渐恢复患者行为康复功能。

关键词：多达理念延续护理干预脑卒中吞咽功能行为功能

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海苔营养物质的核磁共振分析

食品科技 2014年第10期39卷 121-125页

作者：夏梦洁杨锐娄永江叶央芳宁波大学海洋学院宁波315211

海苔是深受广大消费者喜爱的海产加工品,为了分析海苔的营养物质组成,采用基于核磁共振的代谢组学技术对海苔中的物质组成进行了研究。结果表明,海苔主要含有35种营养物质,其中绝大部分是有机羧酸和氨基酸,还含有几种碱类、糖类和糖苷... 详细信息

海苔是深受广大消费者喜爱的海产加工品,为了分析海苔的营养物质组成,采用基于核磁共振的代谢组学技术对海苔中的物质组成进行了研究。结果表明,海苔主要含有35种营养物质,其中绝大部分是有机羧酸和氨基酸,还含有几种碱类、糖类和糖苷类物质以及甜菜碱醛和海带醇。这些物质中含量最丰富的是蔗糖和谷氨酸。

关键词：海苔核磁共振(NMR) 物质组成

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条斑紫菜外生细菌的遗传多样性

海洋学报 2008年第4期30卷 161-168页

作者：杨锐方文雅单媛媛陈海敏孙雪叶央芳宁波大学应用海洋生物技术教育部重点实验室

为研究藻际微生物多样性与紫菜健康养殖的关系,从江苏南通(养殖)、山东青岛(野生)、浙江温州(养殖)三地的条斑紫菜叶状体和江苏东台条斑紫菜贝壳丝状体及其周围的海水样品中分离了条斑紫菜外生细菌。根据菌落特征和菌体形态将获得的63... 详细信息

为研究藻际微生物多样性与紫菜健康养殖的关系,从江苏南通(养殖)、山东青岛(野生)、浙江温州(养殖)三地的条斑紫菜叶状体和江苏东台条斑紫菜贝壳丝状体及其周围的海水样品中分离了条斑紫菜外生细菌。根据菌落特征和菌体形态将获得的63株细菌分为21种表型。16SrDNA-V3片段DGGE分析结合序列测序比对结果表明:这些菌株与假交替单孢菌(Pseudoalter-omonas)、嗜冷单胞菌(Psychrobacter)和芽孢杆菌(Bacillus)等10属相应菌株具有较高的同源性。通过对条斑紫菜外生细菌区系多样性分析得出:条斑紫菜藻体外生细菌组成与生活环境相关,但不同于周围海水细菌群落;不同来源(地点)、不同生理状况(健康与病烂)和不同生长阶段(叶状体和丝状体)的条斑紫菜外生菌种群组成差异较大。许多外生细菌对常见测试菌、海水养殖动物致病菌和人致病菌具有抑菌活性,其抑菌特点与紫菜生理性状相关。从健康条斑紫菜样品分离的外生菌中假交替单孢菌占优势,病烂紫菜未分离到假交替单孢菌,提示假交替单孢菌可能与紫菜健康生长有密切关系。

关键词：条斑紫菜外生细菌遗传多样性抑菌活性

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三疣梭子蟹肠道菌群结构及代谢表型分析

分析化学 2016年第12期44卷 1867-1873页

作者：曾廷兰叶央芳母昌考王凯李荣华王春琳宁波大学应用海洋生物技术教育部重点实验室波谱与原子分子物理国家重点实验室武汉磁共振中心中国科学院武汉物理与数学研究所

建立16S rRNA基因的高通量测序和基于核磁共振（NMR）的代谢组学技术分析三疣梭子蟹肠道菌群结构及代谢物组成的方法。经对梭子蟹肠道样品提取基因组DNA和16 S rRNA基因的V3-V4可变区片段的扩增,产物用于MiSeq测序。经对测序结果进行聚... 详细信息

建立16S rRNA基因的高通量测序和基于核磁共振（NMR）的代谢组学技术分析三疣梭子蟹肠道菌群结构及代谢物组成的方法。经对梭子蟹肠道样品提取基因组DNA和16 S rRNA基因的V3-V4可变区片段的扩增,产物用于MiSeq测序。经对测序结果进行聚类和注释分析,发现梭子蟹肠道的细菌群落结构以变形杆菌门、拟杆菌门、梭杆菌门、软壁菌门和酸杆菌门为主,其中以发光杆菌属、Paludibacter和产丙酸菌属的细菌丰度最高。梭子蟹肠道样品经组织破碎、甲醇-水（2∶1,V/V）提取、高速离心和冷冻干燥后,重悬在钠钾磷酸盐缓冲液中,用于NMR分析。采用标准的Noesypr1D脉冲序列采集;H NMR谱,设置90°脉冲宽度约为10μs,等待时间为2 s,混合时间为100 ms,谱宽为20 ppm,采样点数为32 K,自由感应衰减累加次数为64。再利用2D NMR谱对肠道代谢物进行归属,共获得包括氨基酸、有机羧酸和有机胺类等30种代谢产物。本方法可用于系统分析梭子蟹肠道菌群及其代谢物组成。

关键词：三疣梭子蟹肠道菌群高通量测序代谢表型核磁共振

Inhibitory gamma-aminobutyric acidergic neurons in the anterior cingulate cortex participate in the comorbidity of pain and emotion

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Neural Regeneration Research 2025年第10期20卷 2838-2854页

作者： Lu Guan Mengting Qiu Na Li Zhengxiang Zhou Ru ye Liyan Zhong Yashuang Xu Junhui Ren Yi Liang Xiaomei Shao Jianqiao fang Junfan fang Junying Du Department of Neurobiology and Acupuncture Research Third School of Clinical MedicineZhejiang Chinese Medical UniversityHangzhouZhejiang ProvinceChina Key Laboratory for Research of Acupuncture Treatment and Transformation of Emotional Diseases Third School of Clinical MedicineZhejiang Chinese Medical UniversityHangzhouZhejiang ProvinceChina Fuchun Community Health Service Center of Fuyang District HangzhouZhejiang ProvinceChina

Pain is often comorbid with emotional disorders such as anxiety and *** of the anterior cingulate cortex has been implicated in pain and pain-related negative emotions that arise from impairments in inhibitory gamma-aminobutyric acid *** review primarily aims to outline the main circuitry(including the input and output connectivity)of the anterior cingulate cortex and classification and functions of different gamma-aminobutyric acidergic neurons;it also describes the neurotransmitters/neuromodulators affecting these neurons,their intercommunication with other neurons,and their importance in mental comorbidities associated with chronic pain *** understanding on their role in pain-related mental comorbidities may facilitate the development of more effective treatments for these ***,the mechanisms that regulate gamma-aminobutyric acidergic systems remain *** is also unclear as to whether the mechanisms are presynaptic or *** exploration of the complexities of this system may reveal new pathways for research and drug development.

关键词： anterior cingulate cortex anxiety chronic pain circuit communication comorbidity depression gamma-aminobutyric acidergic neurons parvalbumin neurons synaptic transmission