检索结果-南通市图书馆

Expression and Purification of Goose HSP70 and Compound Formation with Virus Polypeptide

Agricultural Sciences in China 2008年第2期7卷 239-247页

作者： WANG Bao-wei WU Xiao-ping ZHANG Xu-hui jia Xiao-hui ZHANG ming-ai LONG Fang-yu YANG Zhi-gang WANG Lei Water Poultry Graduate School Laiyang Agricultural University Qingdao 266109 P.R.China

The experiment was conducted to study the specific expression of HSP70 caused by heat shock, HSP70 purification and the characteristics of coalescence with antigenic peptide in the formation of the complex. Sixty healthy 6-week-old male Wulong geese were selected and randomly divided into three groups. The control group was slaughtered without heat treatment. Treatment group 1 was shocked with an acute heat treatment at （42 ± 1）℃ for 5 h before they were immediately slaughtered. Treatment group 2 was kept for 12 h after the heat treatment under normal conditions in order to recover and was then slaughtered. Cardiac tissue was taken in order to make paraffin sections for the immunohistochemistry experiment and the liver tissue was used to purify HSP70. The geese heart HSP70 expression differences in the three groups were determined and at the same time the experiments of HSP70 purification and appraisal in the liver tissue were carried on. HSP70 purification and synthesis of HBV PreS1 multi-peptides unified the complex, which was determined by bi-specific antibody enzyme-linked immune sandwich assay. The results indicated that widespread HSP70 positive pellets in the cardiac muscle were found under hot shock conditions. HSP70 expression in the treatment group 1 was centered in the karyotheca and its periphery, but in treatment group 2, it was centered in the surrounding cell membrane. The HSP70 purification could be obtained through two sets of purification plans; both the synthesis peptide and the HSP70 purification form the complex under certain conditions. The double antibody sandwich ELISA technique was applied to detect if the complex had been formed. Positive results showed that the complex was formed. The specific expression of HSP70 under heat shock shifted with time, suggesting that HSP70 possibly had some function in cell protection. High-purity HSP70 protein can be obtained under low-pressure chromatography conditions, and in comparison with each other, it

关键词： heat shock goose HSP70 expression purification appraisal viral multi-peptides complex formation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Glioma stem cells and neural stem cells respond differently to BMP4 signaling

引用

Cell Regeneration 2022年第1期11卷 374-388页

作者： Xin-Xin Han Chunhui Cai Li-ming Yu Min Wang Wenhan Yang Dai-Yu Hu Jie Ren Lu-Ying Zhu jia-jia Deng Qing-Qing Chen Hua He Zhengliang Gao Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases Shanghai Stomatological Hospital&School of StomatologyFudan UniversityShanghai 200001China Yangzhi Rehabilitation Hospital(Shanghai Sunshine Rehabilitation Center) Tongji University School of MedicineShanghai 200072China Institute of Geriatrics(Shanghai University) Affiliated Nantong Hospital of Shanghai University(The Sixth People’s Hospital of Nantong)School of MedicineShanghai UniversityNantongChina School of Medicine Jiax-ing UniversityJiaxingChina Department of Neurosurgery Changzheng HospitalSecond Military Medical UniversityShanghaiChina Department of Neurosurgery Third Affiliated Hospital of Second Military Medical UniversityShanghaiChina

Malignant glioma is a highly heterogeneous and invasive primary brain tumor characterized by high recurrence rates,resistance to combined therapy,and dismal *** stem cells(GSCs)are likely responsible for tumor progression,resistance to therapy,recurrence,and poor prognosis owing to their high self-renewal and tumorigenic *** a family member of BMP signaling,bone morphogenetic protein4(BMP4)has been reported to induce the differentiation of GSCs and neural stem cells(NSCs).However,the molecular mechanisms underlying the BMP4-mediated effects in these two cell types are *** this study,we treated hGSCs and hNSCs with BMP4 and com-pared the phenotypic and transcriptional changes between these two cell ***,we found that the growth of hGSCs was greatly inhibited by BMP4,but the same treatment only increased the cell size of *** the RNA sequencing results showed that BMP4 treatment evoked significantly transcriptional changes in both hGSCs and hNSCs,the profiles of differentially expressed genes were distinct between the two groups.A gene set that specifically targeted the proliferation and differentiation of hGSCs but not hNSCs was enriched and then validated in hGSC *** results suggested that hGSCs and hNSCs responded differently to BMP4 *** and investigating different responses between hGSCs and hNSCs will benefit finding partner factors working together with BMP4 to further suppress GSCs proliferation and stemness without disturbing NSCs.

关键词： Glioma stem cells BMP4 Neural stem cells Cell differentiation Cell proliferation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

超小型水下机器人智能定位系统

引用

控制理论与应用 2010年第9期27卷 1140-1144页

作者：高延增叶家玮陈远明梁富琳华南理工大学船舶与海洋工程系广东广州510641

提出一种新的超小型水下机器人智能定位系统.融合短基线定位声纳、电子罗盘、X/Y倾角传感器和深度传感器组成超小型水下机器人定位的硬件系统,使用基于构件的方法开发了针对超小型水下机器人定位系统的水上控制计算机三维虚拟显示软件.... 详细信息

提出一种新的超小型水下机器人智能定位系统.融合短基线定位声纳、电子罗盘、X/Y倾角传感器和深度传感器组成超小型水下机器人定位的硬件系统,使用基于构件的方法开发了针对超小型水下机器人定位系统的水上控制计算机三维虚拟显示软件.使用马尔可夫自定位算法在实测定位声纳信号的间隙推算水下机器人的位置,提高定位速度,使其与姿态传感器同步.设计了定位算法有效性的测试实验,结果证明算法有效.

关键词：水下机器人马尔可夫自定位软件体系结构构件

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Preparation of Sb2O3/Sb2S3/FeOOH composite photoanodes for enhanced photoelectrochemical water oxidation

引用

Transactions of Nonferrous Metals Society of China 2020年第6期30卷 1625-1634页

作者： Yang PENG jia CHEN Liang-xing jiaNG Tian-yi WANG Hai-chao YANG Fang-yang LIU ming jia School of Metallurgy and Environment Central South UniversityChangsha 410083China BOE Technology Group Co. Ltd.Chengdu 611731China

A novel Sb2O3/Sb2S3/FeOOH photoanode was fabricated via a simple solution impregnation method along with chemical bath deposition and *** X-ray diffractometry,Raman measurement,and X-ray photoelectron spectroscopy show that the Sb2O3/Sb2S3/FeOOH thin films are successfully ***−EDS analyses reveal that the surface of Sb2O3/Sb2S3 thin films becomes rough after the immersion in the FeCl3 *** optimized impregnation time is found to be 8 *** FeOOH co-catalyst loaded Sb2O3/Sb2S3 electrode exhibits an enhanced photocurrent density of 0.45 mA/cm2 at 1.23 V versus RHE under simulated 1 sun,which is approximately 1.41 times compared to the photocurrent density of the unloaded *** the further tests of UV−Vis spectroscopy,the electrochemical impedance spectra,and the PEC measurements,the enhancement can result from the increased light-harvesting ability,the decreased interface transmission impedance,and the remarkably enhanced carrier injection efficiency.

关键词： Sb2O3/Sb2S3 FeOOH co-catalyst photoanode carrier injection efficiency

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

提高我国高校学生体质健康水平的思考

引用

上海海洋大学学报 2010年第1期19卷 140-144页

作者：叶鸣上海海洋大学人文学院上海201306

我国学生体质健康问题已经成为举国上下关心和重视的问题。学校体育教育担负着体育运动、健身技能的传授,学校体育是终身体育的基础,运动兴趣和习惯是促进学生自主学习和终身坚持锻炼的前提,提高学生体质健康是学校体育工作的落脚点,培... 详细信息

我国学生体质健康问题已经成为举国上下关心和重视的问题。学校体育教育担负着体育运动、健身技能的传授,学校体育是终身体育的基础,运动兴趣和习惯是促进学生自主学习和终身坚持锻炼的前提,提高学生体质健康是学校体育工作的落脚点,培养学生终身体育意识是学校体育工作的归宿。通过文献资料法、统计分析法和访问法对上述内容进行研究,本着"为人为本、健康第一"的指导思想,深入的了解高校学生目前的体质健康现状,真正关注学生的健康意识、锻炼习惯和卫生习惯的养成,将增进学生健康贯穿于体育课程实施的全过程,确保"健康第一"的思想落到实处,使学生健康成长。通过多年的第一线教学经验归纳、总结,找到相对适合促进高校学生体质健康发展的主要措施,从而为实现学生身体素质的全面发展取得良好的效果。

关键词：高校学生身体素质体质健康有效措施

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Laparoscopic vs open D2 gastrectomy for locally advanced gastric cancer: A meta-analysis

引用

World Journal of Gastroenterology 2014年第44期20卷 16750-16764页

作者： Zhen-Hong Zou Li-Ying Zhao Ting-Yu Mou Yan-Feng Hu jiang Yu Hao Liu Hao Chen jia-ming Wu Sheng-Li An Guo-Xin Li Department of General Surgery The Second Affiliated Hospital of Nanchang University Department of General Surgery Nanfang Hospital Southern Medical University Department of Biostatistics Southern Medical University

AIM: To conduct a meta-analysis comparing laparoscopic (LGD2) and open D2 gastrectomies (OGD2) for the treatment of advanced gastric cancer (AGC).

关键词： D2 lymph node dissection Gastrectomy Gastric cancer Laparoscopy Meta-analysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

组织芯片制作仪在制作免疫组化外对照中的应用

引用

诊断病理学杂志 2024年第7期31卷 678-679页

作者：贾双双杨冬梅张颖李雅惠李小辰邢莉卫明张勇首都医科大学附属北京同仁医院病理科头颈部分子病理诊断北京市重点实验室北京100730

目的免疫组化染是病理诊断过程中重要的检测手段,设置阳性对照尤其重要。本文旨在探讨一种流程简单的使用组织芯片制作仪制作单组织阳性外对照的方法。方法用组织芯片制作仪中直径2 mm规格的穿刺针在提前备好的空白蜡块上打孔。对照... 详细信息

目的免疫组化染是病理诊断过程中重要的检测手段,设置阳性对照尤其重要。本文旨在探讨一种流程简单的使用组织芯片制作仪制作单组织阳性外对照的方法。方法用组织芯片制作仪中直径2 mm规格的穿刺针在提前备好的空白蜡块上打孔。对照阳性切片的免疫组化染色结果,找出阳性染色部位对应的供体蜡块位置,用直径2 mm规格的穿刺针进行穿刺,并将穿刺出的组织芯注入受体蜡块孔洞中,组织芯片蜡块即制作完成。结果同一张玻片上,单组织阳性对照呈直径2 mm的圆形,与待检组织界限清楚,阳性定位准确,从而确认了试剂的有效性及染色过程的准确性。结论用组织芯片制作仪制作单组织阳性对照流程简单,效果良好,适合有条件的单位日常开展使用。

关键词：组织芯片免疫组化外对照

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

烤烟生产投入间接碳排放分析——以湖北省恩施州烟区为例

引用

江西农业学报 2024年第2期36卷 90-97页

作者：贾明军申国明夏鹏亮黄勇文涛向修志彭五星徐祥玉周剑雄先开远高林中国农业科学院烟草研究所山东青岛266101 中国农业科学院研究生院北京100081 湖北省烟草公司恩施州公司湖北恩施445000 湖北省农业科学院植保土肥研究所湖北武汉430064

以湖北省恩施州烟区为例,通过生产调研与数据统计,对烤烟不同生产阶段(育苗期、大田生长期和采后烘烤期)以及整个生产过程的间接碳排放量进行了核算。结果表明:烤烟生产过程中主要碳源的碳排放量表现为烘烤能源>电力>农膜和肥料>农药、... 详细信息

以湖北省恩施州烟区为例,通过生产调研与数据统计,对烤烟不同生产阶段(育苗期、大田生长期和采后烘烤期)以及整个生产过程的间接碳排放量进行了核算。结果表明:烤烟生产过程中主要碳源的碳排放量表现为烘烤能源>电力>农膜和肥料>农药、机械油耗和人工;采后烘烤期的碳排放量最大,大田生长期的次之,育苗期的最小;在大田生长期,不同肥力和不同种植规模烟田的碳排放总量有所差异,其中由肥料产生的碳排放量占总碳排放量的比例最大;按照烤房类型划分,烤烟整个生产过程的间接碳排放量表现为燃煤型烤房(5167.80 kg C/hm^(2))>生物质能源型烤房(2256.75 kg C/hm^(2))。恩施州烤烟生产中间接碳排放的主要影响因素为烘烤能源、电力,其次为农膜和肥料。因此,用生物质燃料全面替代煤炭作为烘烤能源是烤烟生产减少碳排放的重要途径。

关键词：恩施烤烟生产投入碳排放系数间接碳排放

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

弱聚电解质水凝胶溶胀释药的数值模拟

引用

高等学校化学学报 2018年第4期39卷 817-824页

作者：叶慧刘亚博贾玉玺山东大学材料液固结构演变与加工教育部重点实验室济南250061

在三相模型的基础上,结合Langmuir等温吸附方程和药物释放方程,建立了弱聚电解质水凝胶溶胀释药的数学模型;运用有限元方法,研究了药物在弱聚电解质水凝胶内均匀分布时凝胶体系的材料参数对溶胀及释药性能的影响;分析了水凝胶在不同阶... 详细信息

在三相模型的基础上,结合Langmuir等温吸附方程和药物释放方程,建立了弱聚电解质水凝胶溶胀释药的数学模型;运用有限元方法,研究了药物在弱聚电解质水凝胶内均匀分布时凝胶体系的材料参数对溶胀及释药性能的影响;分析了水凝胶在不同阶段的释药机理;比较了药物在载药基体内的均匀分布和不均匀分布模式对凝胶释药性能的影响,当药物在载药基体内正弦分布时呈现零级释药动力学特点;对比分析了模拟结果与实验结果,验证了弱聚电解质水凝胶溶胀释药模型的准确性.

关键词：弱聚电解质水凝胶溶胀药物释放数学模型有限元模拟

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Correlation of Individual Heterozygosity of Microsatellite Marker Loci with Heterosis of Growth Traits in Pig Populations

引用

Agricultural Sciences in China 2006年第8期5卷 635-642页

作者： ZHANG Jing-hu XIONG Yuan-zhu DENG Chang-yan jiaNG Si-wen LEI ming-gang LI jia-lian LI Feng-e ZHENG Rong Key Laboratory of Swine Genetics and Breeding Ministry of Agriculture/College of Animal Science and Veterinary Medicine Huazhong Agricultural University Wuhan 430070 P.R.China Department of Biology Science ＆ Technology Zhangzhou Teachers College Zhangzhou 363000 P.R.China

To investigate the correlation of individual heterozygosity and heterosis of three traits in crossbred F1 pig populations, the F1 populations were built by random mating Yorkshire x Meishan （YM, n = 82）, and its reciprocal （MY, n =47） and two straightbred populations （Yorkshire = 34, Meishan = 55） were used as control groups. The heterosis of birth weight （BWT）, average daily gain （ADG）, and feed conversion ratio （FCR） were acquired as well. In the research, the significant marker loci for the heterosis of the three traits were observed by one-way ANOVA （P〈0.01） in a total of 39 marker loci on SSC4, SSC6, SSC7, SSC8, and SSC13, and the numbers of the significant marker loci were 12 （BWT）, 18 （ADG）, and 17 （FCR）, respectively, based on which the general heterozygosity （GH） was divided into significant marker loci heterozygosity （SH） and insignificant marker loci heterozygosity （IH）. Furthermore, the trends of alteration in heterosis with the stepwise increase in heterozygosity by 0.05 were explored. This was done by the regression analysis of the three kinds of heterozygosity against heterosis of the three traits. The results showed that, for BWT, the heterosis increased with the increase in GH （r=0.9337, P=0.0021） and SH （r=0.9165, P=0.0102）; for ADG, the heterosis increased with the increase in IH （r=0.7012, P=0.0353） and GH （r=0.7470, P=0.0537, near significant）; for FCR, the heterosis of feed efficiency increased with the increase in IH （r=0.8721, P=0.0022）. The results indicated that the correlation was not always higher or more significant for SH with heterosis than it was for IH or GH with heterosis, and it might be because of the reciprocal cancellation of the positive effect and negative effect of QTL linked to the significant marker loci.

关键词： pig heterosis microsatellite marker individual heterozygosity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：