检索结果-南通市图书馆

Single-cell discrimination based on optical tweezers Raman spectroscopy

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2013年第21期58卷 2594-2600页

作者： MA HongFei ZHANG Yong ye anpei Key Laboratory for the Physics and Chemistry of Nanodevices School of Electronics Engineering and Computer Science Peking University Beijing Institute of Biomedicine

The ability to discriminate between single cells in a label-free and noninvasive fashion is important for the classification of cells, and for the identification of similar cells from different origins. In this paper, we present the Raman spectroscopy-based identifi- cation of different types of single cells in aqueous media, and discrimination between the same types of cells from different donors using a novel Laser Tweezers Raman Spectroscopy (LTRS) technique, which combines laser trapping and micro-Raman spectroscopy. First, we measured the spectra of individual living human erythrocytes, i.e. red blood cells, and leucocytes (U937 cancer cells). High-quality Raman spectra with low fluorescence were obtained using a home-LTRS apparatus and 20 cells were measured for each cell type. The smoothing, baseline subtraction, and normalization of the data were followed by a principal components analysis (PCA). The PCA loading plots showed that the two different types of cells could be completely separated based only on the first component (PC1) (i.e. the peaks at 1300 cm1 ); the discrimination accuracy could therefore reach 100%. More than 50 spectra were taken for each erythrocyte obtained from the four healthy volunteers. The average discrimination accuracy was 84.5% for two random individuals taken from the four volunteers, according to the first and second PCs. This work demonstrates that LTRS is a powerful tool for the accurate identification and discrimination of single cells, and it has the potential to be applied for the highly sensitive identification of cells in clinical diagnosis and medical jurisprudence.

关键词：拉曼光谱技术单细胞激光光镊细胞类型光谱识别显微拉曼光谱测量精度主成分分析

THE EXCESS HEAT EXPERIMENTS ON COLD FUSION IN TITANIUM LATTICE

在线全文

学校读者我要写书评

暂无评论

原子与分子物理学报 1995年第2期12卷 165-169页

作者： Zhang Qingfu Gou Qingquan Zhu Zhenghe Luo Jiaoming Liu Fusheng Sun Jiuxun Miao Bingyou ye anpei Cheng Xianmeng(The Institute of Atomic and Molecular Science at High Temperature and High Pressure Chengdu University of Science and Technology Chengdu 四川大学高温高压与原子分子科学所四川成都

ＴＨＥ　ＥＸＣＥＳＳ　ＨＥＡＴ　ＥＸＰＥＲＩＭＥＮＴＳ　ＯＮ　ＣＯＬＤ　ＦＵＳＩＯＮ　ＩＮ　ＴＩＴＡＮＩＵＭ　ＬＡＴＴＩＣＥＴＨＥＥＸＣＥＳＳＨＥＡＴＥＸＰＥＲＩＭＥＮＴＳＯＮＣＯＬＤＦＵＳＩＯＮＩＮＴＩＴＡＮＩＵＭ... 详细信息

ＴＨＥ　ＥＸＣＥＳＳ　ＨＥＡＴ　ＥＸＰＥＲＩＭＥＮＴＳ　ＯＮ　ＣＯＬＤ　ＦＵＳＩＯＮ　ＩＮ　ＴＩＴＡＮＩＵＭ　ＬＡＴＴＩＣＥＴＨＥＥＸＣＥＳＳＨＥＡＴＥＸＰＥＲＩＭＥＮＴＳＯＮＣＯＬＤＦＵＳＩＯＮＩＮＴＩＴＡＮＩＵＭＬＡＴＴＩＣＥ￥ＺｈａｎｇＱｉｎｇ...

关键词： Cold Fusion Excess Heat Titanium Lattice X-ray diffraction

Measurement of atmospheric nanoparticles:Bridging the gap between gas-phase molecules and larger particles

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2023年第1期123卷 183-202页

作者： Chao Peng Chenjuan Deng Ting Lei Jun Zheng Jun Zhao Dongbin Wang Zhijun Wu Lin Wang Yan Chen Mingyuan Liu Jingkun Jiang anpei ye Maofa Ge Weigang Wang State Key Laboratory for Structural Chemistry of Unstable and Stable Species Beijing National Laboratory for Molecular Sciences(BNLMS)CAS Research/Education Center for Excellence in Molecular SciencesInstitute of ChemistryChinese Academy of SciencesBeijing 100190China State Key Joint Laboratory of Environment Simulation and Pollution Control School of EnvironmentTsinghua UniversityBeijing 100084China School of Environment Science and Engineering Nanjing University of Information Science&TechnologyNanjing 210044China School of Atmospheric Sciences Sun Yat-sen UniversityZhuhaiGuangdong 519082China State Key Joint Laboratory of Environmental Simulation and Pollution Control College of Environmental Sciences and EngineeringPeking UniversityBeijing 100871China Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention(LAP3) Department of Environmental Science&EngineeringFudan UniversityShanghai 200433China Key Laboratory for the Physics and Chemistry of Nanodevices Department of ElectronicsSchool of Electronics Engineering and Computer SciencePeking UniversityBeijing 100871China University of Chinese Academy of Sciences Beijing 100049China

Atmospheric nanoparticles are crucial components contributing to fine particulate matter(PM_(2.5)),and therefore have significant effects on visibility,climate,and human health.Due to the unique role of atmospheric nanoparticles during the evolution process from gas-phase molecules to larger particles,a number of sophisticated experimental techniques have been developed and employed for online monitoring and characterization of the physical and chemical properties of atmospheric nanoparticles,helping us to better understand the formation and growth of new particles.In this paper,we firstly review these state-of-the-art techniques for investigating the formation and growth of atmospheric nanoparticles(e.g.,the gas-phase precursor species,molecular clusters,physicochemical properties,and chemical composition).Secondly,we present findings from recent field studies on the formation and growth of atmospheric nanoparticles,utilizing several advanced techniques.Further-more,perspectives are proposed for technique development and improvements in measuring atmospheric nanoparticles.

关键词： Nanoparticle Measurement techniques Size distributions Physical properties Chemical composition

SEGMENTATION AND 3D RECONSTRUCTION OF ANIMAL LIVER VESSELS BASED ON MR IMAGING

在线全文

学校读者我要写书评

暂无评论

SEGMENTATION AND 3D RECONSTRUCTION OF ANIMAL LIVER VESSELS B...

RAS-RAF蛋白相互作用结构域的单分子力谱分析

第十一届北京分析测试学术报告会暨展览会(BCEIA2...

作者： Yajun WU anpei ye Xiaoying WANG School of EE & CS Peking University Department of Radiology Peking University First Hospital

＜正＞There are more than 5000 children dead from crocking up of liver function because of abnormality of liver.The only way to treat the diseases related to the liver function is the liver transplantation.By now peop...

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

生物物理学报 2011年第11期27卷 941-947页

作者：韩澍闻丞叶安培北京大学信息科学与技术学院纳米器件物理与化学教育部重点实验室北京100871

利用自行构建的光镊系统,通过in vitro单分子力谱测量,在单分子水平研究了RAS/RAF/MEK/ERK信号传导通路中的重要信号传导蛋白RAF与其上游G蛋白RAS结合的势垒形态。RAS与野生型RAF的单分子结合力分布情况显示,RAS-RAF结合至少涉及RAS结合... 详细信息

利用自行构建的光镊系统,通过in vitro单分子力谱测量,在单分子水平研究了RAS/RAF/MEK/ERK信号传导通路中的重要信号传导蛋白RAF与其上游G蛋白RAS结合的势垒形态。RAS与野生型RAF的单分子结合力分布情况显示,RAS-RAF结合至少涉及RAS结合区(RAS binding domain,RBD)及半胱氨酸富集区(cysteine rich domain,CRD)两个作用位点。而RAS与RAF突变体RAF(Q257R)的单分子结合力分布,存在两个以上的结合域:最可几力为95 pN的包络对应RAS-RAF[RBD]结合;最可几力为117 pN的包络对应RAS-RAF[CRD]结合;最可几力为159 pN的包络对应RAS-RAF[RBD]和RAS-RAF[CRD]同时存在(叠加)时的相互作用。而且在大于200 pN的范围(光镊有效量程之外),RAS与RAF(Q257R)的结合力仍然有相当的概率分布。这说明,突变体RAF(Q257R)比其野生型更易于与RAS结合,且可能涉及新的结合位点。该发现对解释Q257R的致病机理及设计拮抗剂均有重要指导意义。

关键词：光镊单分子力谱 RAS-RAF相互作用结构域

Dynamic process research of individual living monocytic U937 cell activated by IL-6 with Raman tweezers

在线全文

学校读者我要写书评

暂无评论

Dynamic process research of individual living monocytic U937...

第十七届国际生物物理大会;第十二届中国生物物理大会

作者： anpei ye~1 Yule Xiong~1 Yin Zheng~1 1.School of Electronic Engineering and Computer Science Peking University Beijing China

Interleukin-6(IL-6) plays an important role in the immune system.It induces growth and differentiation or activation of several human cell lines by triggering a sequence

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

微球透镜超分辨成像研究进展与发展前景

激光与光电子学进展 2016年第7期53卷 19-31页

作者：刘畅金璐頔叶安培北京大学纳米器件物理与化学教育部重点实验室北京100871

受衍射极限的限制,传统光学显微镜的分辨率只能达到入射光波长的一半。超分辨显微镜已有很多,但制作工艺复杂,适用样品有限,对成像条件要求苛刻,因此应用受到很多限制。研究表明,将直径为几微米至几十微米的透明电介质微球置于样品表面... 详细信息

受衍射极限的限制,传统光学显微镜的分辨率只能达到入射光波长的一半。超分辨显微镜已有很多,但制作工艺复杂,适用样品有限,对成像条件要求苛刻,因此应用受到很多限制。研究表明,将直径为几微米至几十微米的透明电介质微球置于样品表面,能显著提高传统光学显微镜的分辨能力,在白光下即可实现超分辨成像,与其他类型显微镜结合使用时也能保持超分辨能力。这种新型透镜为纳米结构和生物样本的实时超分辨成像提供了一种简单、直接的方式。结合本课题组研究结果,介绍并总结了国内外微球透镜的研究进展。

关键词：成像系统超分辨成像微球透镜光子纳米喷射效应生物成像

在线全文

学校读者我要写书评

暂无评论

单颗粒气溶胶液滴吸湿挥发特性研究

大气与环境光学学报 2020年第6期15卷 486-495页

作者：仝玉恺方藤柳瑶瑶赵东平叶安培北京大学信息科学技术学院电子学系纳米器件物理与化学教育部重点实验室北京100871

大气气溶胶粒子的热动力学过程主要源于多元物质的非理想混合,其演化过程包括液-液相分离、吸湿-挥发、非平衡传质等。相关物理化学参数是理解气溶胶演化过程、分析演化动因、预测演化路径的基础,而精确的单粒子测量是获取这些重要参数... 详细信息

大气气溶胶粒子的热动力学过程主要源于多元物质的非理想混合,其演化过程包括液-液相分离、吸湿-挥发、非平衡传质等。相关物理化学参数是理解气溶胶演化过程、分析演化动因、预测演化路径的基础,而精确的单粒子测量是获取这些重要参数的关键。利用自主开发的气溶胶拉曼光镊系统,实现了单颗粒气溶胶液滴无接触长时间捕获,并通过改变气溶胶粒子所处环境的相对湿度,模拟了实际大气中悬浮气溶胶液滴的吸湿-挥发热力学演化过程。通过测量单颗粒液滴粒子的腔共振拉曼光谱信号,结合相应的物理模型精确测量了氯化钠、蔗糖和柠檬酸三种不同气溶胶液滴粒子在吸湿-挥发过程中的粒径、折射率、扩散系数、挥发通量等重要物化参数,分析了有机/无机气溶胶液滴的吸湿-挥发特性对相对湿度变化的不同响应以及气溶胶液滴可能存在的玻璃态、胶态等相变行为,为理解实际大气气溶胶吸湿-挥发过程提供了重要参考。

关键词：气溶胶液滴吸湿性挥发性拉曼光镊

在线全文

学校读者我要写书评

暂无评论

新疆昌吉市灯下昆虫群落结构及时间动态

干旱区资源与环境 2020年第8期34卷 179-187页

作者：张航杨安沛田明昭喜献珍叶新宇李广阔王锁牢新疆农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物综合治理重点实验室乌鲁木齐830091 昌吉市农业技术推广中心昌吉831100

为研究新疆昌吉市灯下昆虫群落结构及多样性,2018年5至9月份在昌吉市滨湖镇应用智能测报灯诱集昆虫,系统调查分析了灯下昆虫群落的结构组成、个体数量、群落特征参数及其时间动态。共收集到昆虫8847头,隶属7目19个类群,灯下植食性昆虫... 详细信息

为研究新疆昌吉市灯下昆虫群落结构及多样性,2018年5至9月份在昌吉市滨湖镇应用智能测报灯诱集昆虫,系统调查分析了灯下昆虫群落的结构组成、个体数量、群落特征参数及其时间动态。共收集到昆虫8847头,隶属7目19个类群,灯下植食性昆虫亚群落中,黑额喙丽金龟累计数量最大,而旋幽夜蛾在各月均为优势类群,灯下捕食/寄生性昆虫亚群落中,草蛉类累计数量最大,且在各月均为优势类群。6、7月份灯下昆虫群落个体数量、类群数量、多样性指数、丰富度指数较高,均匀度指数较低,8月份灯下昆虫群落多样性指数、均匀度指数、丰富度指数均较高,优势度指数最低,9月份灯下昆虫群落个体数量、类群数量、多样性指数、丰富度指数最低,优势度指数最高。灯下昆虫群落、植食性昆虫亚群落结构特征时间动态变化趋势相似,多样性指数、均匀度指数在6月下旬至7月上旬出现明显下降,优势度指数则明显上升。灯下捕食/寄生性昆虫亚群落结构特征时间动态波动较大。

关键词：趋光性昆虫群落结构多样性时间动态