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Small particles,big effects:How nanoparticles can enhance plant growth in favorable and harsh conditions

Journal of Integrative Plant Biology 2024年第7期66卷 1274-1294页

作者： Jie Wang Honghong Wu Yichao Wang wuwei ye Xiangpei Kong Zujun Yin Zhengzhou Research Base State Key Laboratory of Cotton Bio‐breeding and Integrated UtilizationSchool of Agricultural SciencesZhengzhou UniversityZhengzhou 450001China State Key Laboratory of Cotton Bio‐breeding and Integrated Utilization Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyang 455000China National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China School of Engineering Design and Built EnvironmentWestern Sydney UniversityPenrith NSW 2751Australia Western Agricultural Research Center Chinese Academy of Agricultural SciencesChangji 831100China The Key Laboratory of Plant Development and Environmental Adaptation Biology Ministry of EducationCollege of Life SciencesShandong UniversityQingdao 266237China

By 2050,the global population is projected to reach 9 billion,underscoring the imperative for innovative solutions to increase grain yield and enhance food *** has emerged as a powerful tool,providing unique solutions to this ***(NPs)can improve plant growth and nutrition under normal conditions through their high surface-to-volume ratio and unique physical and chemical ***,they can be used to monitor crop health status and augment plant resilience against abiotic stresses(such as salinity,drought,heavy metals,and extreme temperatures)that endanger global *** of NPs can enhance stress tolerance mechanisms in plants,minimizing potential yield losses and underscoring the potential of NPs to raise crop yield and *** review highlights the need for a comprehensive exploration of the environmental implications and safety of nanomaterials and provides valuable guidelines for researchers,policymakers,and agricultural *** thoughtful stewardship,nanotechnology holds immense promise in shaping environmentally sustainable agriculture amid escalating environmental challenges.

关键词： abiotic stress agricultural defense system nano-particles nanotechnology oxidative stress reactive oxygen species toxicity

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“大思政课”视阈下高校辅导员工作课程化探析

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赣南师范大学学报 2024年第2期45卷 115-120页

作者：叶芜为赣南师范大学教育科学学院江西赣州341000

党和国家推动“大思政课”建设给高校辅导员工作课程化研究和实践提供了历史性机遇。基于“大思政课”背景,推进高校辅导员工作课程化实践具有辅导员身份、职能和发展变革诉求的大逻辑,具备辅导员供优课、能上课和讲道理核心功能的大价... 详细信息

党和国家推动“大思政课”建设给高校辅导员工作课程化研究和实践提供了历史性机遇。基于“大思政课”背景,推进高校辅导员工作课程化实践具有辅导员身份、职能和发展变革诉求的大逻辑,具备辅导员供优课、能上课和讲道理核心功能的大价值,需要辅导员重在落实、基于事实和成在课改行动力的大担当。倡导辅导员工作课程化,绝非推动专职辅导员兼职化的历史性倒退,也不是促使专职辅导员专任教师化的转型,而是试图创设一种融辅导员“干部+教师”双重身份、“工作+教育”双重职能和“职称+职务”双重晋升于一体的途径和方法,形成有利于辅导员潜下心来育人、静下心来教育教学的制度环境,建设一支职业化专业化专家化辅导员队伍,使他们真正成长为学生心目中具有“大先生”情怀、教育家精神的知心朋友和人生导师。

关键词：大思政课辅导员工作课程化双重身份

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中国乡村创新创业发展:指数构建与测度分析

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经济与管理 2024年第5期38卷 9-18页

作者：阮建青杨奇明叶武威张雨薇浙江大学中国农村发展研究院浙江杭州310058 浙江理工大学经济管理学院浙江杭州310018 企研数据科技(杭州)有限公司浙江杭州310018

鼓励乡村创新创业是推进乡村振兴战略的重要抓手,但尚未有科学严谨的研究全面揭示中国乡村创新创业的发展程度和时空特征。通过构建包括2个一级指标、7个二级指标和21个三级指标的乡村创新创业指标体系,计算2014—2021年中国县、市、省... 详细信息

鼓励乡村创新创业是推进乡村振兴战略的重要抓手,但尚未有科学严谨的研究全面揭示中国乡村创新创业的发展程度和时空特征。通过构建包括2个一级指标、7个二级指标和21个三级指标的乡村创新创业指标体系,计算2014—2021年中国县、市、省级三个层面的乡村创新创业指数,并对其发展趋势、空间特征等展开分析。研究发现:中国乡村创新创业总体上蓬勃发展,但区域间存在差异;乡村振兴示范县的创新创业程度要显著高于其他县,国家重点帮扶县的创新创业增长速度要显著高于非重点帮扶县;乡村创新创业活动存在明显的空间集聚现象,同时也表现出区域收敛的趋势。乡村创新创业指数已向社会开放,将为各界研究中国乡村创新创业提供基础性数据。

关键词：乡村创新创业指标体系空间集聚区域收敛重点帮扶县

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Genome-wide identification and expression analysis of DNA demethylase family in cotton

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Journal of Cotton Research 2019年第3期2卷 142-150页

作者： YANG Xiaomin LU Xuke CHEN Xiugui WANG Delong WANG Junjuan WANG Shuai GUO Lixue CHEN Chao WANG Xiaoge WANG Xinlei ye wuwei Institute of Cotton Research of Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton Biology Key Laboratory for Cotton Genetic ImprovementMOAA

Background:DNA methylation is an important epigenetic factor that maintains and regulates gene *** mode and level of DNA methylation depend on the roles of DNA methyltransferase and demethylase,while DNA demethylase plays a key role in the process of DNA *** results showed that the plant’s DNA demethylase all contained conserved DNA glycosidase *** study identified the cotton DNA demethylase gene family and analyzed it using bioinformatics methods to lay the foundation for further study of cotton demethylase gene ***:This study used genomic information from diploid Gossypium raimondii JGI(D),Gossypium arboreum ***(A),Gossypium hirsutum ***(AD1) and Gossypium barbadebse L NAU(AD2) to Arabidopsis *** DNA demethylase genes sequence of Arabidopsis as reference,25 DNA demethylase genes were identified in cotton by BLAST *** are 4 genes in the genome D,5 genes in the genome A,10 genes in the genome AD1,and 6 genes in the genome *** gene structure and evolution were analyzed by bioinformatics,and the expression patterns of DNA demethylase gene family in Gossypium hirsutum L were *** the phylogenetic tree analysis,the DNA demethylase gene family of cotton can be divided into four subfamilies:REPRESSOR of SILENCING 1(ROS1),DEMETER(DME),DEMETER-LIKE 2(DML2),and DEMETER-LIKE3(DML3).The sequence similarity of DNA demethylase genes in the same species was higher,and the genetic relationship was also relatively *** of the gene structure revealed that the DNA demethylase gene family members of the four subfamilies varied *** them,the number of introns of ROS1 and DME subfamily was larger,and the gene structure was more *** the analysis of the conserved domain,it was known that the DNA demethylase family gene member has an endonuclease Ⅲ(END03 c) ***:The genes of the DNA demethylase family are distributed differently in different cotton species,and the ge

关键词： DNA demethylase Gossypium arboreum L. Gossypium barbadebse L. Gossypium hirsutum L. Gossypium raimondii

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Characters and structures of the nucleobase-ascorbate transporters(NAT)family genes in Gossypium hirsutum and their roles in responding to salt and drought stresses

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Journal of Cotton Research 2022年第2期5卷 115-129页

作者： GUO Lixue ZHAO Lanjie LU Xuke CHEN Xiugui WANG Shuai WANG Junjuan WANG Delong YIN Zujun CHEN Chao FAN Yapeng ZHANG Yuexin ye wuwei State Key Laboratory of C otton Biology/I nstitute of Cott on Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base School of Agricultural SciencesZhengzhou University/Key Laboratory for Cotton Genetic ImprovementMOARAAnyang 455000HenanChina

Background: Nucleobase-ascorbate transporters(NAT), synonymously called nucleobase-cation symporter 2(NCS2) proteins, were earlier reported to be involved in plant growth, development and resistance to stress. Previous studies concluded that s a polymorphic SNP associated with NAT12 was significant di erent between salt-tolerant and salt-sensitive materials of upland cotton. In current study, a comprehensive analysis of NAT family genes was conducted for the first time in ***: In this study, we discovered 32, 32, 18, and 16 NAT genes in Gossypium hirsutum, G. barbadense, G. raimondii and G. arboreum, respectively, which were classified into four groups(groups I–IV) based on the multiple sequence analysis. These GhNAT genes were unevenly distributed on At and Dt sub-genome in G. hirsutum. Most GhNAT members in the same group had similar gene structure characteristics and motif composition. The collinearity analysis revealed segmental duplication as well as tandem duplication contributing to the expansion of the GhNATs. The analysis of cis-acting regulatory elements of GhNATs showed that the function of GhNAT genes in cotton might be related to plant hormone and stress response. Under di erent conditions, the expression levels further suggested the GhNAT family genes were associated with plant response to various abiotic stresses. GhNAT12 was detected in the plasma membrane. And it was validated that the GhNAT12 gene played an important role in regulating cotton resistance to salt and drought stress through the virus-induced gene silencing(VIGS) ***: A comprehensive analysis of NAT gene family was performed in cotton, including phylogenetic analysis, chromosomal location, collinearity analysis, motifs, gene structure and so on. Our results will further broaden the insight into the evolution and potential functions of NAT genes in cotton. Current findings could make significant contribution towards screening more candidate genes related to b

关键词： Gossypium hirsutum NAT gene family VIGS Salt and drought stresses

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Zinc finger transcription factor ZAT family genes confer multi-tolerances in Gossypium hirsutum L.

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Journal of Cotton Research 2021年第3期4卷 268-281页

作者： FAN Yapeng ZHANG Yuexin RUI Cun XU Nan ZHANG Hong WANG Jing MALIK Waqar Afeal HAN Mingge ZHAO Lanjie LU Xuke CHEN Xiugui CHEN Chao ye wuwei Key Laboratory of Cotton Biology Institute of Cotton Research of Chinese Academy of Agricultural SciencesAnyang 455000HenanChina

ZAT(Zinc Finger of Arabidopsis thaliana)proteins are composed of a plant-specific transcription factor family,which play an important role in plant growth,development,and stress resista *** study the potential function of ZAT family in cotton,the whole genome identification,expression,and structure analysis of ZAT gene family were carried *** this study,our analysis revealed the presenee of 115Z 56,59,and 115 ZAT genes in Gossypium hirsutum,G raimondii,*** and G barbadense,*** to the number of domains and phylogenetic characteristics,we divided ZAT genes of four Gossypium species into 4 different clades,and further divided them into 11 *** results of collinearity an alysis showed that segmental duplicati on was the main method to amplify the cotton ZAT genes *** of c/s-elements of promoters indicated that most GhZAT genes contained c/5-elements related to plant hormones and abiotic *** to heatmap analysis,the expression patterns of GhZAT genes under different stresses indicated that GhZAT genes were significantly involved in the response to cold,heat,salt,and PEG stress,possibly through different *** the highly expressed genes,we cloned a G hirsutum gene *** virus-induced gene silencing(VIGS),we found that its expression level decreased significantly after being *** alkaline treatment,the wilting degree of silenced plants was even greater than the wild type,which proved that GhZAT67 gene was involved in the response to alkaline stress.

关键词： ZAT Phylogenetic analysis Collinearity analysis c/s-element VIGS

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Identification and Characterization of Salt-responsive MicroRNAs and Their Target Genes in Cotton

Identification and Characterization of Salt-responsive Micro...

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2015年全国棉花青年学术研讨会

作者： Yin Zujun Wang Junjuan Wang Delong Fan Weili Wang Shuai ye wuwei State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese Academy of Agricultural Sciences

Soil salinity is one of the most serious factors limiting the productivity of agricultural *** salt concentrations in the soil affect various aspects of plant growth and development,such as inhibition of enzymatic activities and reductions in photosynthetic *** respond to stress using multiple gene regulatory mechanisms including post-transcriptional regulation of gene ***（miRNAs）,an abundant class of short non-coding（21-23 nt） RNAs,are generated from single-strand hairpin RNA precursors and are sometimes conserved over long evolutionary *** regulate gene expression by binding to targeted mRNA sequences in a perfect or near-perfect complementary site,leading to mRNA cleavage or,in a few cases,translational repression in *** this study,three cotton small RNA libraries were constructed and sequenced under normal conditions,short-term salt stress（SS） and long-term salt stress（LS）.The length distribution of obtained small RNAs was significantly different between *** SS sample showed significantly higher 21-nt small RNA abundance（48.86%） than CK（40.63%） and the LS sample（36.31%）.To investigate the repertoire of miRNAs,small RNA reads in three small RNA libraries were first mapped to protein-coding and structural RNA-coding regions in the Rfam and GenBank ***,tRNAs,snRNAs and snoRNAs were ***,the remaining sequences were aligned with all known miRNAs in the central miRNA Registry Database,miRBase *** a result,a total of 201 conserved miRNAs were *** were classified into 88 families on the basis of sequence *** remaining non-encoding sequences were used to predict novel miRNAs using the MIREAP program,based on their secondary *** 24 of them,the complementary miRNA species were cloned in libraries,providing an indication of precise excision from the stem-loop *** first made a comparative analysis of miRNA expression between CK and SS lib

关键词： cotton microRNAs target genes ARFs

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Identification and Expression Analysis Mitogen-Activated Protein Kinase Signalling Cascades in Cotton

Identification and Expression Analysis Mitogen-Activated Pro...

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中国农学会棉花分会40年征文暨2019年年会

作者： Zhu Weidong Wang Junjuan Wang Delong Wang Shuai Guo Lixue Yin Zujun ye wuwei State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese Academy of Agricultural Sciences

Mitogen-activated protein kinase(MAPK) cascades are involved in plant growth and development,as well as play a central role in response to biotic and abiotic ***,few complete MAPK signaling cascades have been characterized in *** this study,a total of 169 MAPKKK,25 MAPKK and 56 MAPK genes were identified in Gossypium hirsutum genome and classified into distinct groups supported by the phylogenetic *** structures and conserved motifs analyses pointed out that the majority of MAPKKK,MAPKK and MAPK genes in the same subfamily shared similar gene structures and conserved ***,analyses of cis-acting regulatory elements in promoter regions revealed that MAPK cascades might participate in plant responses to diverse abiotic stresses and ***,the different stages of transcriptome analysis demonstrated that 39 MAPKKK genes,10 MAPKK genes and 20 MAPK genes play an important role in drought stress *** the similarity of expression patterns could indicate the correlation and the similar biological function of *** addition,interaction networks analyses of three gene families suggest that MAPK signalling cascade mediate network participates in multiple stress *** are some high confidence interactive proteins involved in MAPKKK,MAPKK and MAPK networks,including MEKK1,WRKY33,MKKs,and ***,our study will provide a useful reference for further functional analysis of MAPK cascades in cotton.

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Comparative Analysis of Salinity-Induced Proteomic Changes in Cotton (Gossypium hirsutum L.)

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Agricultural Sciences 2015年第1期6卷 78-86页

作者： Yupeng Cui Xuke Lu Delong Wang Junjuan Wang Zujun Yin Weili Fan Shuai Wang wuwei ye State Key Laboratory of Cotton Biology Institute of Cotton Research of Chinese Academy of Agricultural Sciences Anyang China

Salt stress on cotton varieties of distinct salinity tolerance can induce expression of different proteins. Zhong 07, a salt-tolerant variety and Zhong s9612, a salt-sensitive variety, were utilized as experimental materials. The leaves of trefoil seedlings treated with or without 0.4% NaCl for 24 h were harvested for whole-protein extraction. Two-dimensional technology, combined with mass spectroscopy (MS) analysis and protein database searching, was employed to detect differentially expressed proteins and determine their identities and biological functions. Compared with the control, Zhong 07 showed 10 differentially expressed proteins under salt stress, of which 6 were upregulated and 4 were downregulated. Meanwhile, 12 differentially expressed proteins were detected in Zhong s9612 under salt stress, of which 10 were upregulated and 2 were downregulated. In the matrix-assisted laser desorption-ionization/time of flight-time of flight/MS analysis, 14 differentially expressed proteins were successfully identified, including the ribulose-1, 5-bisphosphate carboxylase/oxygenase (RuBisco) large subunit-binding protein subunit alpha (RuBisco α), luminal binding protein (LBP), heat shock protein 70 (Hsp1, 2, 3), pathogenesis-related protein class 10 (PR-10), quinoneoxidoreductase-like protein (QOR), S-adenosylmethioninesyn-thetase (SAMS), enolase (EN), and RuBisco large subunit-binding protein subunit beta (RuBisco β). Cellular function is ultimately executed by proteins, and cotton varieties with different salt tolerance can be influenced by salt stress to various degrees, which can provide certain theoretical foundation for the identification of salt tolerance of cotton varieties. The findings also provide some proteins, such as the RuBisco large subunit binding proteins α and β subunits, OEE2 protein, HSP70, and S-adenosylmethionine synthetase, which can be used as protein markers of salt-to-lerance before- and post-treatment, making a big difference in salt-toleranc

关键词： Upland Cotton (Gossypium hirsutum L.) Salt Stress Differential Protein Mass Spectrum Analysis

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Transcriptome-Wide Identification of Salt-Responsive miRNA Targets in Cotton Using Degradome Sequencing

Transcriptome-Wide Identification of Salt-Responsive miRNA T...

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中国农学会棉花分会2016年年会

作者： Yin Zujun Wang Junjuan Wang Delong Fan Weili Wang Shuai Guo Lixue ye wuwei State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese Academy of Agricultural Sciences

＜正＞MicroRNAs（miRNAs）are a class of endogenous,non-coding small RNAs that play important roles in many developmental processes and stress responses in *** finely modulate gene expression by binding to targeted mRN...

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