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A genetic linkage map with 178 SSR and 1901 SNP markers constructed using a RIL population in wheat (Triticum aestivum L.)

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Journal of Integrative Agriculture 2015年第9期14卷 1697-1705页

作者： ZHAI Hui-jie FENG Zhi-yu LIU Xin-ye CHENG Xue-jiao PENG Hui-ru yao ying-yin SUN Qi-xin NI Zhong-fu State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Heterosis and Utilization Ministry of Education/Key Laboratory ofCropGenomicsandGeneticImprovementMinistryofAgriculture/BeijingKeyLaboratoryofCropGeneticImprovementNational Plant Gene Research Centre (Beijing)China Agricultural University

The construction of high density genetic linkage map provides a powerful tool to detect and map quantitative trait loci(QTLs) controlling agronomically important traits. In this study, simple sequence repeat(SSR) markers and Illumina 9K i Select single nucleotide polymorphism(SNP) genechip were employed to construct one genetic linkage map of common wheat(Triticum aestivum L.) using 191 recombinant inbred lines(RILs) derived from cross Yu 8679×Jing 411. This map included 1 901 SNP loci and 178 SSR loci, covering 1 659.9 c M and 1 000 marker bins, with an average interval distance of 1.66 c M. A, B and D genomes covered 719.1, 703.5 and 237.3 c M, with an average interval distance of 1.66, 1.45 and 2.9 c M, respectively. Notably, the genetic linkage map covered 20 chromosomes, with the exception of chromosome 5D. Bioinformatics analysis revealed that 1 754(92.27%) of 1 901 mapped SNP loci could be aligned to 1 215 distinct wheat unigenes, among which 1 184(97.4%) were located on o ne single chromosome, and the rest 31(2.6%) were located on 2 to 3 chromosomes. By performing in silico comparison, 214 chromosome deletion bin-mapped expressed sequence tags(ESTs), 1 043 Brachypodium genes and 1 033 rice genes were further added onto the genetic linkage map. This map not only integrated genetic and physical maps, SSR and SNP loci, respectively, but also provided the information of Brachypodium and rice genes corresponding to 1 754 SNP loci. Therefore, it will be a useful tool for comparative genomics analysis, fine mapping of QTL/gene controlling agronomically important traits and marker-assisted selection breeding in wheat.

关键词： wheat genetic linkage map SNP SSR unigene deletion bin-mapped ESTs

Wheat 14-3-3 Protein Conferring Growth Retardation in Arabidopsis

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Journal of Integrative Agriculture 2013年第2期12卷 209-217页

作者： LI Jing SONG Su-sheng ZHAO Yu-sheng GUO Wei-wei GUO Guang-hui PENG Hui-ru NI Zhong-fu SUN Qi-xin yao ying-yin State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization Minstry of Education/Key Laboratory of Crop Genomics and Genetic Improvement Minstry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement China Agricultural University School of Life Sciences Tsinghua University

14-3-3 proteins belong to a family of phosphoserine/threonine-binding modules and participate in a wide array of signal transduction and regulatory events. Our previous study demonstrated that Ta14-3-3 was significantly down-regulated in leaf and root tissues of hybrid wheat at the tillering stage. In this paper, three homoeologous Ta14-3-3 genes were cloned from common wheat （Triticum aestivum L., 2n=6x=42, AABBDD） and mapped on chromosomes 2A, 2B, and 2D, respectively. Transgenic Arabidopsis plants ectopically overexpressing Ta14-3-3 displayed shorter primary roots, delayed flowering and retarded growth rates, indicating that Ta14-3-3 acted as a growth inhibitor in Arabidopsis. In wheat, Ta14-3-3 was down-regulated in roots and leaves of hybrids as compared to their parental lines. We proposed that Ta14-3-3 proteins might regulate growth vigor in hybrid wheat.

关键词： wheat Ta14-3-3 heterosis gene expression

Research on Credit Regulation Mechanism of E-commerce Platform Based on Evolutionary Game Theory

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Journal of Systems Science and Systems Engineering 2024年第3期33卷 330-359页

作者： Zeguo Qiu Yuchen yin yao Yuan Yunhao Chen School of Computer and Information Engineering Harbin University of CommerceHarbin150028China School of Management Harbin University of CommerceHarbin150028China

In the growing e-commerce industry,the problem of malicious business operations has become increasingly prominent,exposing many problems such as weak supervision of e-commerce platforms and no way for consumers to *** order to solve the problems of counterfeit and shoddy products on e-commerce platforms and promote the sustainable development of the e-commerce industry in China,this paper constructs a three-party evolutionary game model of e-commerce platforms,merchants,and consumers,investigates the influence of each influencing factor on each party’s strategy choice,and provides targeted suggestions to e-commerce platforms based on relevant ***,the impact of several important parameters on the equilibrium solution is discussed through sensitivity *** results show that:1)the smaller the cost difference between active and negative regulation,the more the e-commerce platform tends to active regulation strategy,but increasing fines for dishonest merchants and consumer complaints have little impact on the e-commerce platform;2)increasing consumer compensation,creating an honest business environment,and reducing the cost of honest business all help companies tend to operate in good faith;3)the only factor that affects the tendency of consumer complaint strategies is the cost of *** loss suffered by silence and the compensation given to consumers have little effect on consumers’tendency to complain *** results can provide theoretical guidance for participants to make useful strategic decisions in the e-commerce market.

关键词： Evolutionary game e-commerce data simulation

A robust viscoelastic surfactant tolerating 20% HCl up to 150℃ for oil well stimulation

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Petroleum Science 2024年第3期21卷 1865-1874页

作者： Ji Wang Ning Qi Hong-yao yin Yu-Jun Feng Polymer Research Institute State Key Laboratory of Polymer Materials EngineeringSichuan UniversityChengdu610065SichuanPR.China Tianfu Yongxing Laboratory New Theory and Technology of CO_(2) Capture Research CenterChengdu610065SichuanPR China School of Petroleum Engineering China University of Petroleum(East China)Qingdao266580ShandongPR China

Viscoelastic surfactants(VES)are often used as viscous diverters in acidizing stimulation to prolong the acid consumption time and maximize zonal coverage of the acid for improving well ***,the ceiling temperature of commercial VES cannot exceed 120℃in practical use because of the poor thermal stability and fragile molecular structure,hindering their implementation in hightemperature oil reservoirs,i.e.,≥150℃.Here we synthesized a novel C22-tailed diamine,N-erucaminopropyl-N,N-dimethylamine(EDPA),and examined comparatively its rheological behavior,assemblies morphology and molecular stability in 20 wt%HCl with a commercial VES,erucyl dimethyl amidopropyl betaine(EDAB).The feasibility of EDPA for acidizing stimulation was assessed by acid etching of carbonate rock with its HCl solution at 150℃.Rheological results showed that the 2.5 wt%EDPA—20 wt%HCl solution maintains stable viscosity of 90 m Pa s at 150℃for 60 min,while that of 2.0 wt%EDAB HCl solution is just 1 m Pa s under identical conditions.1H NMR spectra and cryo-TEM observations revealed that the chemical structure and self-assembled architectures of EDPA remained intact in such context,but the EDAB suffered from degradation due to the hydrolysis of the amide group,accounting for the poor heat-resistance and *** reaction rate of 2.5 wt%EDPA HCl solution with carbonate rock was one order of magnitude lower than that of 20 wt%HCl solution at 150℃,underpinning the potential of EDPA to be used in the high-temperature reservoirs *** work improved the thermal tolerance of VES in highly concentrated HCl solution,paving a feasible way for the acidization of high-temperature reservoir environments(~150℃).

关键词： Viscoelastic surfactants Thermal stability Self-diverting acid Wormlike micelles Acidizing stimulation

Global output feedback regulation of time-varying nonlinear systems via the dual-gain method

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Science China(Information Sciences) 2024年第5期67卷 333-334页

作者： Xian-Long yin Zong-yao SUN Changyun WEN Institute of Automation Qufu Normal University School of Electrical and Electronic Engineering Nanyang Technological University

Notably, owing to the limited information available, the problem of output feedback stabilization/regulation for nonlinear systems has been explored based on state *** addition, the sensors accounting for state measurement could fail to detect the system states accurately due to the limitations of manufacturing techniques and instruments.

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Comparison of microwave- and thermal-assisted rock fragmentation methods at different temperatures and loading rates

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International Journal of Mining Science and Technology 2024年第6期34卷 799-819页

作者： Wei yao Shuai Wang Bangbiao Wu ying Xu Kaiwen Xia State Key Laboratory of Hydraulic Engineering Intelligent Construction and Operation School of Civil EngineeringTianjin UniversityTianjin 300072China China Railway Design Corporation Tianjin 300308China Institute of Geosafety School of Engineering and TechnologyChina University of GeosciencesBeijing 100083China

Understanding the effects of microwave irradiation and thermal treatment on the dynamic compression and fragmentation properties of rocks is essential to quantify energy consumption in rock *** this study,Fangshan granite(FG)specimens were exposed to microwave irradiation and heat *** damage of FG specimens induced by these two methods was compared using X-ray CT scanning and ultrasonic wave *** temperatures of FG after microwave irradiation and thermal treatment were effectively evaluated using a newly proposed technique.A novelty method for precisely determining the geometric features of fragments is developed to estimate the fragmentation ***,the dynamic uniaxial compressive strength(UCS),the dynamic fragmentation characteristics,and the fragmentation energy of FG after these two pretreatment methods can be reasonably *** noticeable distinction of loading rate effect on the dynamic UCS of FG between these two pretreatment methods is first observed.A relationship is established between the dynamic UCS and the damage induced by microwave irradiation and heat ***,fragmentation energy fan analysis is introduced to accurately compare the fragmentation properties of FG after two pretreatment methods in dynamic compression tests.

关键词： Fangshan granite Dynamic experiments Microwave irradiation Thermal treatment Loading rate Fragmentation

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Research on intelligent search-and-secure technology in accelerator hazardous areas based on machine vision

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Nuclear Science and Techniques 2024年第4期35卷 96-107页

作者： ying-Lin Ma yao Wang Hong-Mei Shi Hui-Jie Zhang School of Traffic and Transportation Beijing Jiaotong UniversityBeijing 100044China School of Mechanical Electronic and Control EngineeringBeijing Jiaotong UniversityBeijing 100044China Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China

Prompt radiation emitted during accelerator operation poses a significant health risk,necessitating a thorough search and securing of hazardous areas prior to ***,manual sweep methods are ***,the limitations of manual sweeps have become increasingly evident with the implementation of large-scale *** leveraging advancements in machine vision technology,the automatic identification of stranded personnel in controlled areas through camera imagery presents a viable solution for efficient search and *** the criticality of personal safety for stranded individuals,search and security processes must be sufficiently *** ensure comprehensive coverage,180°camera groups were strategically positioned on both sides of the accelerator tunnel to eliminate blind spots within the monitoring *** YOLOV8 network model was modified to enable the detection of small targets,such as hands and feet,as well as larger targets formed by individuals near the ***,the system incorporates a pedestrian recognition model that detects human body parts,and an information fusion strategy is used to integrate the detected head,hands,and feet with the identified pedestrians as a cohesive *** strategy enhanced the capability of the model to identify pedestrians obstructed by equipment,resulting in a notable improvement in the recall ***,recall rates of 0.915 and 0.82were obtained for Datasets 1 and 2,*** there was a slight decrease in accuracy,it aligned with the intended purpose of the search-and-secure software *** tests conducted within an accelerator tunnel demonstrated the effectiveness of this approach in achieving reliable recognition outcomes.

关键词： Search and secure Machine vision Camera Human body parts recognition Particle accelerator Hazardous area

Trends and Age-Period-Cohort Effect on Incidence of Varicella Under Age 35—China,2005-2021

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China CDC weekly 2024年第18期6卷 390-395,I0005页

作者： Guijie Luan Hongyan yao Dapeng yin Jianjun Liu Department of Education and Training Chinese center for Disease Control and PreventionBeijingChina Hainan Provincial Center for Disease Control and Prevention Haikou CityHainan ProvinceChina Chinese center for Disease Control and Prevention BeijingChina

Summary What is already known about this topic?Varicella is an acute respiratory infectious disease primarily affecting ***,recent studies have indicated an increasing susceptibility to varicella among older age *** is added by this report?The findings demonstrate a significant rise in the incidence rate among individuals aged *** under 20 years old were found to have a higher risk compared to females,whereas males had a lower risk compared to females aged 20-35 years.

关键词： acute respiratory Incidence

Gradient-based algorithms for multi-objective bi-level optimization

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Science China Mathematics 2024年第6期67卷 1419-1438页

作者： Xinmin Yang Wei yao Haian yin Shangzhi Zeng Jin Zhang National Center for Applied Mathematics in Chongqing Chongqing401331China School of Mathematical Sciences Chongqing Normal UniversityChongqing401331China Department of Mathematics Southern University of Science and TechnologyShenzhen518055China National Center for Applied Mathematics Shenzhen Shenzhen518000China Department of Mathematics and Statistics University of VictoriaVictoriaBCV8W 2Y2Canada

Multi-objective bi-level optimization(MOBLO)addresses nested multi-objective optimization problems common in a range of ***,its multi-objective and hierarchical bi-level nature makes it notably ***-based MOBLO algorithms have recently grown in popularity,as they effectively solve crucial machine learning problems like meta-learning,neural architecture search,and reinforcement ***,these algorithms depend on solving a sequence of approximation subproblems with high accuracy,resulting in adverse time and memory complexity that lowers their numerical *** address this issue,we propose a gradient-based algorithm for MOBLO,called gMOBA,which has fewer hyperparameters to tune,making it both simple and ***,we demonstrate the theoretical validity by accomplishing the desirable Pareto *** experiments confirm the practical efficiency of the proposed method and verify the theoretical *** accelerate the convergence of gMOBA,we introduce a beneficial L2O(learning to optimize)neural network(called L2O-gMOBA)implemented as the initialization phase of our gMOBA *** results of numerical experiments are presented to illustrate the performance of L2O-gMOBA.

关键词： multi-objective bi-level optimization convergence analysis Pareto stationary learning to optimize