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Circular RNA TLK1 aggravates neuronal injury and long-term neurological deficits via miR-335-3p/TIPARP after ischemic stroke

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中国药理学与毒理学杂志 2018年第9期32卷 676-677页

作者： WU Fang-fang yao hong-hong Department of Pharmacology School of MedicineSoutheast University Department of Neurology Zhongda HospitalNeuropsychiatric InstituteSchool of MedicineSoutheast University

OBJECTIVE Circular RNAs(circRNAs) are highly expressed in the brain and involved in various central nervous system ***,the potential role of circRNAs in ischemic brain injury remains largely *** present study investigated the functional significance of a circular RNA TLK1(circTLK1) in cerebral ischemic *** The expression of circTLK1,neurological deficit scores,infarct volume,and long-term neurological deficits were examined in a rodent model of middle cerebral artery occlusion using real-time polymerase chain reaction,triphenyltetrazolium chloride staining,magnetic resonance imaging,GolgiCox staining and behavior *** injury was undertaken in primary cultured cortical neurons challenged by oxygen-glucose deprivation using Western blotting and *** interaction between circTLK1 and miR-335-3 p was examined using fluorescence in situ hybridization and affinity isolation *** downstream target of miR-335-3 p-TIPARP was determined by luciferase assay and Western blotting *** CircTLK1 levels were significantly increased in brain tissues in a mouse model of focal cerebral ischemia and *** of circTLK1 expression significantly decreased infarct volumes,attenuated neuronal injury,and improved subsequent long-term neurological ***,circT LK1 functions as an endogenous microRNA-335-3 p sponge to inhibit miR-335-3 p activity,resulting in the inhibition of 2,3,7,8-tetrachlorodibenzo-p-dioxin-inducible polymerase expression and subsequent attenuation of neuronal *** studies confirmed the up-regulation of circT LK1 in plasma of patients with ischemic *** CircTLK1 and its underlying mechanisms are involved in ischemic brain injury,suggesting circTLK1 as a potential therapeutic target for ischemic stroke.

关键词： circular RNA TLK1 miR-335 neuronal injury stroke

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Engagement of circular RNA HECW2 in non-autophagic role of ATG5 implicated in endothelial-mesenchymal transition

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中国药理学与毒理学杂志 2018年第9期32卷 736-737页

作者： YANG Li HAN Bing ZHANG Yuan BAI Ying CHAO Jie HU Gang yao hong-hong Department of PharmacologySchool of Medicine Southeast University Nanjing 210009 China Department of Physiology School of Medicine Southeast University Nanjing 210009 China Jiangsu Key Laboratory of Neurodegeneration Department of Pharmac

Endothelial-mesenchymal transition(EndoMT) is associated with damage to blood-brain barrier(BBB) *** RNAs(circRNAs) are highly expressed in the brain and are involved in brain diseases;however,whether circR NAs regulate the EndoM T in the brain remains *** study demonstrated that circH ECW2 regulated the EndoM T by directly binding to MIR30 D,a significantly downregulated miR NA from miR NA profiling,which subsequently caused an increased expression of *** findings shed new light on the understanding of the noncanonical role of ATG5 in the EndoMT induced by methamphetamine or *** in vivo relevance was confirmed as microinjection of circHecw2 siRNA lentivirus into the mouse hippocampus suppressed the EndoMT induced by *** findings provide novel insights regarding the contribution of circHECW2 to the nonautophagic role of ATG5 in the EndoMT process in the context of drug abuse and the broad range of neuroinflammatory disorders.

关键词： ATG5 circular RNA HECW2 EndoMT MIR30D nonautophagy

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Ⅱ、Ⅲ组亲代谢型谷氨酸受体激动剂逆转1-甲基-4-苯基吡啶离子抑制C6胶质瘤细胞摄取谷氨酸

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中国临床药理学与治疗学 2005年第10期10卷 1096-1099页

作者：王芳姚红红胡刚南京医科大学药理学与神经生物学系

目的：探讨Ⅱ、Ⅲ组亲代谢型谷氨酸受体(metabotropic glutamate receptors，mGluRs)激动剂对1-甲基-4-苯基吡啶离子(1-metyl-4-phenylpyridinium，MPP^+)抑制C6胶质瘤细胞摄取谷氨酸(glutamate，Glu)的影响。方法：应用同位素标记法测定C6胶质瘤细胞对培养液中[~3H]-D，L-谷氨酸的摄取。结果：Ⅱ组mGluRs激动剂(2S，2’R，3’R)-2-(2’，3’-di-carboxycyclopropyl)glycine(DCG-Ⅳ)和Ⅲ组mGluRs激动刺L(+)-2-amino-4-phosphonobutyric acid(LAP4)100μmol·L^(-1)可以逆转MPP^+抑制C6胶质瘤细胞摄取Glu的作用，而Ⅱ组mGluRs拮抗剂(RS)-1-Amino-5-phosphonoinan-1-carboxylic acid(APICA)和Ⅲ组mGluRs拮抗剂(RS)-α-methvlserine-O-phosphate(MSOP)可以完全阻断其激动剂的逆转作用。结论：激活C6胶质瘤细胞上的Ⅱ、Ⅲ组mGluRs可以通过促进谷氨酸转运体摄取Glu、进而降低细胞外液的Glu浓度。

关键词：亲代谢型谷氨酸受体 1-甲基-4-苯基吡啶离子 C6胶质瘤细胞谷氨酸摄取

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II、III组亲代谢型谷氨酸受体激动剂对脂多糖抑制C6胶质瘤细胞摄取谷氨酸的影响

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中国临床药理学与治疗学 2007年第6期12卷 626-629页

作者：邹静姚红红胡刚南京医科大学药理学系

目的:探讨II、III组亲代谢型谷氨酸受体(metabotropic glutamate receptors,mGluRs)激动剂对脂多糖(LPS)抑制C6胶质瘤细胞摄取谷氨酸(gluta-mate,Glu)的影响。方法:应用同位素标记法测定C6胶质瘤细胞对[3H]-D,L-Glu的摄取。应用Ho-echst染色法、噻唑蓝比色法(MTT)分别检测C6胶质瘤细胞的凋亡、细胞活力。结果:LPS(4、6μg/mL)显著抑制C6胶质瘤细胞摄取[3H]-D,L-Glu,抑制率分别达17.6%和22.2%。II组mGluRs激动剂DCG-IV100μmol/L和III组mGluRs激动剂L-AP4100μmol/L逆转LPS对C6胶质瘤细胞摄取[3H]-D,L-Glu的抑制作用,这种逆转作用分别被II、III组mGluRs拮抗剂APICA和MSOP取消。结论:DCG-IV和L-AP4逆转LPS引起的C6胶质瘤细胞Glu摄取抑制,提示II、III组mGluRs激动剂通过促进Glu摄取,降低细胞外Glu浓度,从而发挥神经保护作用。

关键词：亲代谢型谷氨酸受体脂多糖 C6胶质瘤细胞谷氨酸摄取

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Opening of microglial K-ATP channels inhibits rotenone-induced neuroinflammation

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南京医科大学学报（自然科学版） 2008年第12期28卷 1612-1612页

作者： Zhou, Fang yao, hong-hong Wu, Jia-Yong Ding, Jian-Hua Sun, Tao Hu, Gang Nanjing Med Univ Dept Anat Histol & Pharmacol Jiangsu Key Lab Neurodegenerat Nanjing 210029 Jiangsu Peoples R China

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Drosophila models used to simulate human ATP1A1 gene mutations that cause Charcot-Marie-Tooth type 2 disease and refractory seizures

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Neural Regeneration Research 2025年第1期20卷 265-276页

作者： yao Yuan Lingqi Yu Xudong Zhuang Dongjing Wen Jin He Jingmei hong Jiayu Xie Shengan Ling Xiaoyue Du Wenfeng Chen Xinrui Wang Institute of Life Sciences College of Biological Science and EngineeringFuzhou UniversityFuzhouFujian ProvinceChina NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate(Fujian Maternity and Child Health Hospital) FuzhouFujian ProvinceChina Medical Research Center Fujian Maternity and Child Health HospitalCollege of Clinical Medicine for Obstetrics&Gynecology and PediatricsFujian Medical UniversityFuzhouFujian ProvinceChina Department of Neurology and Institute of Neurology of The First Affiliated Hospital Institute of Neuroscienceand Fujian Key Laboratory of Molecular NeurologyFujian Medical UniversityFuzhouFujian ProvinceChina

Certain amino acids changes in the human Na^(+)/K^(+)-ATPase pump,ATPase Na^(+)/K^(+)transporting subunit alpha 1(ATP1A1),cause Charcot-Marie-Tooth disease type 2(CMT2)disease and refractory *** develop in vivo models to study the role of Na^(+)/K^(+)-ATPase in these diseases,we modified the Drosophila gene homolog,Atpα,to mimic the human ATP1A1 gene mutations that cause *** located within the helical linker region of human ATP1A1(I592T,A597T,P600T,and D601F)were simultaneously introduced into endogenous Drosophila Atpαby CRISPR/Cas9-mediated genome editing,generating the Atpα^(TTTF)*** addition,the same strategy was used to generate the corresponding single point mutations in flies(Atpα^(I571T),Atpα^(A576T),Atpα^(P579T),and Atpα^(D580F)).Moreover,a deletion mutation(Atpα^(mut))that causes premature termination of translation was generated as a positive *** these alleles,we found two that could be maintained as homozygotes(Atpα^(I571T)and Atpα^(P579T)).Three alleles(Atpα^(A576T),Atpα^(P579)and Atpα^(D580F))can form heterozygotes with the Atpαmut *** found that the Atpαallele carrying these CMT2-associated mutations showed differential phenotypes in *** heterozygous for Atpα^(TTTF)mutations have motor performance defects,a reduced lifespan,seizures,and an abnormal neuronal *** Drosophila models will provide a new platform for studying the function and regulation of the sodium-potassium pump.

关键词： ATP1A1 Atpα bang-sensitive paralysis Charcot-Marie-Tooth disease type 2 CRISPR/Cas9 homology-directed repair Na^(+)/K^(+)-ATPase point mutation seizures sodium pump

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测定麦芽糖转糖苷反应体系组成的高效液相色谱法

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分析测试学报 2002年第3期21卷 34-36页

作者：邹耀洪居红芳陈爱英常熟高等专科学校生物化学系江苏常熟215500

建立了分析低聚异麦芽糖组成的高效液相色谱法 ,采用Spherisorb_NH2色谱柱,示差折光检测器 ,乙腈 -水 (体积比70∶30)为流动相 ,外标法定量测定 ;结果显示各糖质量浓度在0.1～10g/L范围内与峰面积呈良好线性关系 ,相关系数为0.9990～0.9... 详细信息

建立了分析低聚异麦芽糖组成的高效液相色谱法 ,采用Spherisorb_NH2色谱柱,示差折光检测器 ,乙腈 -水 (体积比70∶30)为流动相 ,外标法定量测定 ;结果显示各糖质量浓度在0.1～10g/L范围内与峰面积呈良好线性关系 ,相关系数为0.9990～0.9997 ;应用该法跟踪了pH5.0的柠檬酸 -柠檬酸钠缓冲溶液中以α_葡萄糖转苷酶为催化剂在58℃温度下的麦芽糖转糖苷反应 ,分析了反应体系组成随时间的变化 ,得到了上述反应条件下麦芽糖最大限度地转化为低聚异麦芽糖的最佳反应时间为24h;该法快捷、简单、准确 ,可用于低聚异麦芽糖生产的质量控制。

关键词：测定转糖苷反应组成低聚异麦芽糖转糖苷作用高效液相色谱

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A STUDY OF E-CADHERIN AND B-CATENIN EXPRESSION AND THEIR CORRELATION WITH PROGNOSIS OF ADENOCARCINOMA OF THE GALLBLADDER

A STUDY OF E-CADHERIN AND B-CATENIN EXPRESSION AND THEIR COR...

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第十一届国际体视学大会暨第十届中国体视学与图像分析学术会议

作者： hong yao, hong WANG, Xinyan LI, Mali ZHAO, Suping PING, Qingsheng ZHANG, Zhihong NIE, Qiusuo WANG, Qunli SHI Depratment of Pathology, Taiyuan People’s Hospital, Taiyuan 030001,china

Objective: To investigate the protein and mRNA expression of E-cadherin and P -catenin in adenocarcinoma of the gallbladder and to find their correlation with differentiation, metastasis, TNM and prognosis. Methods: High sensitive sp immunohistochemical method and in situ hibridization were used to detect the protein and mRNA expression of E-cadherin and β-catenin. Results: Imm- unohistochemistry revealed that among the 60 *** positive rates of E-cadherin and β-catenin were 40% and 45% *** abnormal exprssion rates of these two proteins were 58.3% and 63.3% *** was no significant relationship between E-cadherin and β-catenin staining and histological type of the tumor(p>0.05). However, there was a statistically significant difference between well and moderately differentiated cells and poorly differentiated cells(p<0.05. In cases with lymphatic *** abnormal expression rates of E-cadherin and β-catenin were significantly higher than those in nonmetastatic cases(p<0.05=.The mean survivaltime in cases with abnormal E-cadherin and β-catenin expression were significantly shorter than thatin cases with the expression grading(++) - (+++). In situ hybridization showed that in adenocarcinoma of the *** positive rate of E-cadherin and β-catenin mRNA was 60% and 40% respectively. Their concordant rates with(++)-(+++) protein expression were 78.8% and 82.4%,***: The concordant rate of E-cadherin and β-catenin mRNA and protein expression was relatively high. They can be used as markers of prognosis of adenocarcinoma of the gallbladder in clinical practice.

关键词： Adenocarcinoma of the gallbladder E-cadherin β -catenin prognosis Immunohistochemistry in situ hibridization

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3D Printing of Tough Hydrogel Scaffolds with Functional Surface Structures for Tissue Regeneration

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Nano-Micro Letters 2025年第2期17卷 18-45页

作者： Ke yao Gaoying hong Ximin Yuan Weicheng Kong Pengcheng Xia Yuanrong Li Yuewei Chen Nian Liu Jing He Jue Shi Zihe Hu Yanyan Zhou Zhijian Xie Yong He State Key Laboratory of Fluid Power and Mechatronic Systems School of Mechanical EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China Liangzhu Laboratory Zhejiang UniversityHangzhou 310027People’s Republic of China Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province College of Mechanical EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China Stomatology Hospital School of StomatologyZhejiang University School of MedicineHangzhou 310027People’s Republic of China State Key Laboratory of Advanced Welding and Joining Harbin Institute of TechnologyHarbin 150001People’s Republic of China Institute of Digital Medicine Nanjing First HospitalNanjing Medical UniversityNanjing 210006People’s Republic of China

Hydrogel scaffolds have numerous potential applications in the tissue engineering ***,tough hydrogel scaffolds implanted in vivo are seldom reported because it is difficult to balance biocompatibility and high mechanical *** by Chinese ramen,we propose a universal fabricating method(printing-P,training-T,cross-linking-C,PTC&PCT)for tough hydrogel scaffolds to fill this ***,3D printing fabricates a hydrogel scaffold with desired structures(P).Then,the scaffold could have extraordinarily high mechanical properties and functional surface structure by cycle mechanical training with salting-out assistance(T).Finally,the training results are fixed by photo-cross-linking processing(C).The tough gelatin hydrogel scaffolds exhibit excellent tensile strength of 6.66 MPa(622-fold untreated)and have excellent ***,this scaffold possesses functional surface structures from nanometer to micron to millimeter,which can efficiently induce directional cell ***,this strategy can produce bionic human tissue with mechanical properties of 10 kPa-10 MPa by changing the type of salt,and many hydrogels,such as gelatin and silk,could be improved with PTC or PCT *** experiments show that this scaffold can effectively promote the new generation of muscle fibers,blood vessels,and nerves within 4 weeks,prompting the rapid regeneration of large-volume muscle loss injuries.

关键词： 3D printing Tough hydrogel scaffold Functional surface structure Tissue regeneration Biomaterials

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基于OSG的飞行器实时监控软件设计

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计算机技术与发展 2017年第4期27卷 21-24页

作者：郝朝药红红山寿中国飞行试验研究院陕西西安710089

在飞行试验中,飞行器实时监控是保障飞行安全和提高工作效率的一个关键环节。为了直观地实时监控飞行器的位置姿态和工作状态,保证飞行器试飞安全,提出了基于OSG(Open Scene Graph)技术的飞行器三维实时监控软件设计方案。软件采用C++... 详细信息

在飞行试验中,飞行器实时监控是保障飞行安全和提高工作效率的一个关键环节。为了直观地实时监控飞行器的位置姿态和工作状态,保证飞行器试飞安全,提出了基于OSG(Open Scene Graph)技术的飞行器三维实时监控软件设计方案。软件采用C++语言设计,读取飞行器的部件模型构建三维可视化飞行场景,通过TCP/IP协议与服务器完成数据交互,实时驱动飞行场景中模型运动,设计独立线程响应键盘和鼠标操作,采用场景漫游和模型选取技术,通过三维模型运动与数字显示相结合的方式,更加可视化地监控飞行器的飞行过程。利用模块化和多线程的设计思路,提高了软件的执行效率和可扩展性。目前该软件已成功应用到多个型号任务试飞中。实际应用效果表明,该监控软件具有良好的实时性、准确性、可靠性和人机交互功能,能够满足飞行器实时监控系统的要求。

关键词： OSG 飞行器实时监控多线程人机交互

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