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Inhibiting ceramide synthase 5 expression in microglia decreases neuroinflammation after spinal cord injury

Neural Regeneration Research 2025年第10期20卷 2955-2968页

作者： Wei Zhang Yubao Lu Ruoqi Shen Yingjie Wu Chenrui Liu Xingxing Fang Liangming Zhang Bin Liu Limin rong Department of Spine Surgery The Third Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina National Medical Products Administration(NMPA)Key Laboratory for Quality Research and Evaluation of Cell Products GuangzhouGuangdong ProvinceChina Guangdong Provincial Center for Quality Control of Minimally Invasive Spine Surgery GuangzhouGuangdong ProvinceChina Guangdong Provincial Center for Engineering and Technology Research of Minimally Invasive Spine Surgery GuangzhouGuangdong ProvinceChina

Microglia,the resident monocyte of the central nervous system,play a crucial role in the response to spinal cord ***,the precise mechanism remains *** investigate the molecular mechanisms by which microglia regulate the neuroinflammatory response to spinal cord injury,we performed single-cell RNA sequencing dataset analysis,focusing on changes in microglial *** found that the MG1 subpopulation emerged in the acute/subacute phase of spinal cord injury and expressed genes related to cell pyroptosis,sphingomyelin metabolism,and neuroinflammation at high ***,we established a mouse model of contusive injury and performed intrathecal injection of siRNA and molecular inhibitors to validate the role of ceramide synthase 5 in the neuroinflammatory responses and pyroptosis after spinal cord ***,we established a PC12-BV2 cell co-culture system and found that ceramide synthase 5 and pyroptosis-associated proteins were highly expressed to induce the apoptosis of neuron *** ceramide synthase 5 expression in a mouse model of spinal cord injury effectively reduced ***,ceramide synthase 5-induced pyroptosis was dependent on activation of the NLRP3 signaling *** ceramide synthase 5 expression in microglia in vivo reduced neuronal apoptosis and promoted recovery of neurological ***2g7 formed a“bridge”between sphingolipid metabolism and ceramide synthase 5-mediated cell death by inhibiting the NLRP3 signaling ***,these findings suggest that inhibiting ceramide synthase 5 expression in microglia after spinal cord injury effectively suppressed microglial pyroptosis mediated by NLRP3,thereby exerting neuroprotective effects.

关键词： ceramide synthase 5 gasdermin D microglia neuroinflammation NLRP3 nuclear factor kappa B Pla2g7 pyroptosis sphingomyelin metabolism spinal cord injury

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COMPARISON OF RESULTS ON CAVITATION INCEPTION FOR CHECKING THE SCALE EFFECTS

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Journal of Hydrodynamics 2004年第3期16卷 308-311页

作者： yangzhi-ming DINGYu-jian WuhanUniversity Wuhan430072China

Results on scale effects for cavitation inception at small and large Reynoldsnumbers were compared, respectively. The empirical relation for scale effects suggested by *** Keller was checked, which showed that the comparison of results on cavitation inception atsmall Reynolds numbers below 1 x 10~6 was confirmed very well, but at larger Reynolds numbers abovethe magnitude of 1 x 10~6 was not clearly confirmed.

关键词： cavitation inception scale effects reynolds numbers

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Analysis on Dynamic Characteristics in Time Domain of Slider with Ultra Thin Spacing

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Chinese Journal of Mechanical Engineering 2012年第3期25卷 439-445页

作者： YAO Huaping HUANG Ping CHEN yangzhi ZHU Lixue School of Mechanical and Electrical Engineering Zhongkai University of Agriculture and EngineeringGuangzhou 510225China School of Mechanical and Automobile Engineering South China University of TechnologyGuangzhou 510640China

With increasing recording density in computer hard disks,the flying height of the magnetic head becomes 10 nm or *** numerical method of the control equations needs to be optimized consistently with the decrease of the flying *** iterative process is usually divergent with traditional methods which cannot ensure the accuracy of the analysis results and the stability of numerical ***,a new scheme is presented to solve the ultra-thin lubricating control equations in this *** equilibrium equation is obtained by taking the Couette flow terms and time term as dominating terms in iterative ***,the weighting flow factor is introduced for the abrupt change of film thickness in order to reduce calculated ***,the rules for variation of flying pose of the magnetic head with time are obtained by the time domain *** results show that the magnetic head can recover to the equilibrium position after being slightly influenced by outside *** the dynamic lubrication properties of the head suffer heavily from the vibrations in the height direction and fluctuation in the pitch ***,the analysis of the sine roughness-passing capacity of the head indicates that the head can surpass small sine waves and the equilibrium position can be retrieved after a period of decaying *** proposed research ensures the accuracy and stability of the numerical calculation of the control equations,and the dynamic characteristics and flying stability of a magnetic head is studied *** can also be the theoretical basis for the analysis of the flight characteristics of the slider.

关键词： dynamic characteristic ultra-thin spacing dynamic response magnetic head

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Numerical model of flow and pollutant transportation in non-orthogonal curvilinear coordinates

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Journal of Environmental Sciences 2004年第2期16卷 293-296页

作者： WUXiu-guang SHENYong-ming ZHENGYong-hong yangzhi-feng StateKeyLaboratoryofCoastalandOffshoreEngineering DalianUniversityofTechnologyDalian116024China. GuangzhouInstituteofEnergyConversion ChineseAcademyofSciencesGuangzhou510070China InstituteofEnvironmentalScience BeijingNormalUniversityBeijing100875China

The planar 2D k-ε double equations' turbulence model was adopted and transformed into non-orthogonal curvilinear coordinates. The concentration convection-diffusion was introduced to planar 2D SIMPLEC algorithm of flow in non-orthogonal curvilinear coordinates. The numerical model of pollutant transportation in non-orthogonal curvilinear coordinates was constructed. The model was applied to simulate the flow and pollutant concentration fields. In the testing concentration field, two optimal operations of contamination discharging both along bank and in the centerline at the first bend of the meandering channel were adopted. Comparison with available data showed the model developed was successful, was valuable to engineering application.

关键词： curvilinear coordinates diffusion-transportation SIMPLEC algorithm concentration fields meandering channel

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Full composition granules of Huanglian (Rhizoma Coptidis) decrease the serum monocyte chemotactic protein-1 and connective tissue growth factor levels and inhibit kidney nuclear factor-κB expression in rats with high-fat diet-induced diabetes

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Journal of Traditional Chinese Medicine 2021年第3期41卷 424-431页

作者： GUO yangzhi DU Juan JIANG Min GUO Wei Department of Traditional Chinese Medicine Capital Medical University Affiliated Beijing Shijitan HospitalBeijing 100038China Department of Obstetrics and Gynecology Beijing Changping Hospital of Integrated Chinese and Western MedicineBeijing 102208China Department of Traditional Chinese Medicine the Mongolian Medical and Traditional Chinese Medicine Hospital of WuhaiWuhai 016000China

OBJECTIVE: To investigate the efficacy of the full composition granules of Huanglian(Rhizoma Coptidis)(FGC) on the serum monocyte chemotactic protein-1(MCP-1) and connective tissue growth factor(CTGF) levels and kidney nuclear factor-κB(NF-κB) expression in rats with high-fat diet-induced ***: Diabetes was induced in rats by feeding a high-fat chow combined with intravenous streptozotocin injection. Forty diabetic SpragueDawley rats were randomly assigned to a normal group(NG), model group(MG), irbesartan group(IG), and low-, middle-, and high-dosage FGC groups(LFGC, MFGC, HFGC), with eight rats per group. The IG rats received 31.25 mg·kg^(-1)·d^(-1) irbesartan tablets, whereas those in the LFGC, MFGC,and HFGC were administered 52, 312.5, and 625 mg·kg^(-1)·d^(-1) FGC, respectively. After 12 weeks,bodyweight(BW), left kidney weight(KW), hemoglobin A1c(HbA1c), serum creatinine(Scre), blood urea nitrogen(BUN), and serum MCP-1 and CTGF levels were determined, pathological changes of the kidney were recorded, and kidney NF-κB p65(A) expression was ***: The 24-h urine albumin and levels of HbA1c, Scre, BUN, and serum MCP-1 and CTGF were significantly increased in in the MG compared with the NG, as was the kidney NF-κB(p65) expression(P < 0.05). Furthermore, clear pathological changes in kidney fibrosis were observed in the MG rats. Following irbesartan and FGC administration,the 24-h urine albumin and the levels of HbA1c,Scre, and serum MCP-1 and CTGF were significantly decreased in FCG groups compared with those in the MG, which is in agreement with the change in the kidney NF-κB(p65) expression, whereas the similarly significant decrease only exist in 24-h urine albumin and the levels of serum CTGF after irbesartan administration. Hematoxylin-eosin(HE)staining results indicated that the fibrosis observed in the MG samples was alleviated through FGC ***: FGC may alleviate potential kidney injury by decreasing the serum MCP-1 an

关键词： Huanglian(Rhizoma Coptidis) diabetic nephropathies chemokine CCL2 connective tissue growth factor NF-kappa B

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Research on buildings impacting on aerosol diffusing in urban area using remote sensing

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Journal of Environmental Sciences 2004年第3期16卷 509-512页

作者： YANGSheng-tian yangzhi-feng MAOXian-qiang ZHUQi-jiang InstituteofEnvironment BeijingNormalUniversityBeijiug100875China CenterforRS＆GIS BeijingNormalUniversityBeijing100875.China

Employing remote sensing method to interpret the building volumetric ratio and aerosol status in Guangzhou, China. The relation between them and identified characteristics of their spatial distribution was analyzed. Results showed that building density and aerosol status are strongly correlated. It is indicated that the resistance of building to aerosol diffusing is one of factors influencing air pollution in urban area. On the basis of calculated results, building voluminous ratio of 5 6 is taken as the threshold impacting on aerosol diffusing, so the building voluminous ratio of Guangzhou should be limited to less than 5 6 in order to alleviate air pollution.

关键词： remote sensing aerosol building voluminous ratio air pollution

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Recent Development of Air-Cooled Bainitic Steels Containing Manganese

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Journal of Iron and Steel Research International 2005年第2期12卷 1-10页

作者： FANGHong-sheng YANGFubao BAIBing-zhe yangzhi-gang YINJiang TsinghuaUniversity Beijing100084China GeneralResearchInstituteforNonferrousMetals Beijing100088China BaosteelGroupShanghaiNo.5SteelCoLtd Shanghai200940China

The superiorities of air-cooled bainitic steels were described. A series of air-cooled bainitic steels containing manganese were developed and presented, which include low carbon granular bainitic steels, low carbon grain-boundary allotriomorphic ferrite/granular bainite dual phase steels, medium and medium high carbon bainite/martensite dual phase steel, low carbon carbide free bainite/martensite dual phase steels and casting bainitic steels. The development of ultra-low carbon bainitic steels in China was also introduced.

关键词： bainite bainitic steel strength toughness

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A Role-Based PMI Security Model for E-Government

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Wuhan University Journal of Natural Sciences 2005年第1期10卷 329-332页

作者： WULi-jun SUKai-le yangzhi-hua DepartmentofComputerScienceandTechnology ZhongshanUniversityGuangzhou510275GuangdongChina

We introduce the general AC( atlribure certificate), the role specificationAC and the rolt assignment AC We discuss the rolt-based PMI(Privilege Management Infrastructure)architecture. The role-based PMT(Public-Kty In-frastructure) secure model forE-govcrnment isresearehed by combining the role-bastd PMI with PKI architeclure (Public Key Infrastructure). Themodel has advantages of flexibility, convenience, less storage space and less network consumptionetc. We are going to ust iht secure modelin the E-govern-ment system.

关键词： E-govemment public key certificate role-specification AC role-assignmentAC ihe security model for E- government

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Effects of interlinker sequences on the biological properties of bispecific single-chain antibodies

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Chinese Science Bulletin 2003年第21期48卷 2277-2283页

作者： FANGMin JIANGXin yangzhi YINChangcheng LIHua ZHAORui ZHANGZhong LINQing HUANGHualiang InstituteofGeneticsandDevelopmentalBiology ChineseAcademyofSciencesBeijing100101China PekingUniversitySchoolofOncology&BeijingCancerHospital Beijing100036China

Single-chain bispecific antibody (scBsAb) is one of the promising genetic engineering antibody formats for clinical application. But the effects of interlinker sequences on the biological properties of bispecific single-chain antibodies have not been studied in detail. Three interlinker sequences were designed and synthesized, and denominated as Fc, HSA, 205C′, respectively. Universal vectors with these different interlinker sequences for scBsAb expression in E. coli were constructed. A model scBsAb based on a reshaped single-chain antibody (scFv) against human CD3 and a scFv directed against human ovarian carcinoma were generated and expressed in E. coli. The results of SDS-PAGE and Western blot showed that the different interlinker sequences did not affect the expression level of scBsAb. However, as demonstrated by ELISA and pharmacokinetics studies performed in mice, scBsAbs with different interlinker sequences had difference in the antigen-binding activities and terminal half-life time (T 1÷2β) in vivo, the interlinker HSA could remarkably prolong the retention time of scBsAb in blood. These results indicated that the peptide sequence of interlinker could affect important biological properties of scBsAb, such as antigen-binding properties and stability in vivo. So, selection of an appropriate interlinker sequence is very important for scBsAb construction. Optimal interlinker can bring scBsAb biological properties more suitable for clinical application.

关键词：单链双榍抗体基因工程临床应用生物学连环序列

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Interaction of Hydrogen and Retained Austenite in Bainite/Martensite Dual-Phase High Strength Steel

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Journal of Iron and Steel Research International 2004年第1期11卷 42-46页

作者： GUJia-lin CHANGKai-di FANGHong-sheng yangzhi-gang BAIBing-zhe TsinghuaUniversity Beijing100084China ShougangResearchInstituteofTechnology Beijing100041China

The hydrogen trapping phenomena in two bainite/martensite dual-phase high strength steels(U20Si and U20DSi)were investigated by electrochemical permeation *** hydrogen diffusivity was calculated from data of permeation delay time,and the diffusion coefficient in U20 Si is far less than that in U20 ***,the hydrogen diffusivity decreases as the volume percent of retained austenite *** experiment results show that there are different hydrogen trappings and different volume percents of retained austenite in U20 Si and U20 *** retained austenite is precipitated as *** trap binding energy for the retained austenite and hydrogen is calculated to be 40.4kJ·mol-1.

关键词： bainite martensite dual-phase high strength steel electrochemical permeation technique diffusion coefficient retained austenite trap binding energy

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