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Agricultural Sciences in China 2011年第11期10卷 1665-1680页

作者： TANG Yong-lu LI Jun wu Yuan-qi WEI Hui-ting LI Chao-SU yang wu-yun CHEN Fang Crop Research Institute Sichuan Academy of Agricultural Sciences Chengdu 610066 P.R.China College of Life Sciences Sichuan University Chengdu 610064 P.R.China Agronomy College Sichuan Agricultural University Ya ＇an 625014 P.R. China

Synthetic hexaploid wheat （SHW） represents a valuable source of new resistances to a range of biotic and abiotic stresses. A recombinant inbred line （RIL） population with 127 recombinant inbred lines derived from a SHW-derived variety Chuanmai 42 crossing with a Chinese spring wheat variety Chuannong 16 was used to map QTLs for agronomic traits including grain yield, grains per square meter, thousand-kernel weight, spikes per square meter, grain number per spike, grains weight per spike, and biomass yield. The population was genotyped using 184 simple-sequence repeat （SSR） markers and 34 sequence-related amplified polymorphism （SRAP） markers. Of 76 QTLs （LOD〉2.5） identified, 42 were found to have a positive effect from Chuanmai 42. The QTL ***-4D.2 associated with grain yield on chromosome 4D was detected in four of the six environments and the combined analysis, and the mean yield, across six environments, of individuals carrying the Chuanmai 42 allele at this locus was 8.9% higher than that of those lines carrying the Chuannong 16 allele. Seven clusters of the yield-coincident QTLs were detected on 1A, 4A, 3B, 5B, 4D, and 7D.

关键词： yield-related traits quantitative trait loci Chuanmai 42 synthetic hexaploid wheat

Prognostic value of high-sensitivity cardiac troponin T in patients with en-domyocardial-biopsy proven cardiac amyloidosis

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Journal of Geriatric Cardiology 2014年第2期11卷 136-140页

作者： Geng QIAN Chen wu yang ZHANG yun-Dai CHEN Wei DONG Yi-Hong REN Department of Cardiology Chinese PLA General Hospital 28 Fuxing Road Beijing 100853 China

Objective To investigate prognostic predictors of long-term survival of patients with cardiac amyloidosis （CA）, and to determine predictive value of high-sensitivity cardiac troponin T （hs-cTnT） in CA patients. Methods We recruited 102 consecutive CA cases and followed these patients for 5 years. We described their clinical characteristics at presentation and used a new, high-sensitivity assay to determine the concentration of cTnT in plasma samples from these patients. Results The patients with poor prognosis showed older age （56 ±12 years vs. 50 ±15 years, P=0.022）, higher incidences of heart failure （36.92%vs. 16.22%, P=0.041）, pericardial effusion （60.00%vs. 35.14%, P=0.023）, greater thickness of interventricular septum （IVS）（15 ±4 mm vs. 13 ±4 mm, P=0.034）, higher level of hs-cTnT （0.186 ±0.249 ng/mL vs. 0.044 ±0.055 ng/mL, P=0.001） and higher NT-proBNP （N-terminal pro-B-type natriuretic pep-tide） levels （11,742 ± 10,464 pg/mL vs. 6,031 ± 7,458 pg/mL, P=0.006）. At multivariate Cox regression analysis, heart failure （HR：1.78, 95%CI：1.09-2.92, P=0.021）, greater wall thickness of IVS （HR：1.44, 95%CI：1.04-3.01, P=0.0375） and higher hs-cTnT level （HR：6.16, 95%CI：2.20-17.24, P=0.001） at enrollment emerged as independent predictors of all-cause mortality. Conclusions We showed that hs-cTnT is associated with a very ominous prognosis, and it is also the strongest predictor of all-cause mortality in multivariate analysis. Examination of hs-cTnT concentrations provides valuable prognostic information concerning long-term outcomes.

关键词： Cardiac amyloidosis Long-term survival Troponin T

Distribution and Selective Effects of Vrn-A1,Vrn-B1,and Vrn-D1 Genes in Derivative Varieties from Four Cornerstone Breeding Parents of Wheat in China

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Agricultural Sciences in China 2010年第10期9卷 1389-1399页

作者： ZHANG yang LIU Wan-chen LI Jun WEI Hui-ting HU Xiao-rong LI Yue-jian LU Bao-rong yang wu-yun Crop Research Institution Sichuan Academy of Agricultural Scienee Chengdu 610066 P.R. China School of Life Science Sichuan University Chengdu 610064 P.R.China Institution of Biodiversity Science Fudan University Shanghai 200433 P.R.China

Vernalization, the process of a long exposure to low temperature to induce flowering in plants, is essential for plants to adapt to cold winters. The presence of vernalization genes Vrn-A1, -B1, and -D1 in four cornerstone breeding parents of wheat in China （Funo, Mentana, Yanda 1817, and Bima 4） and 322 derivative varieties （mostly winter wheat） from these parents were determined using PCR based molecular method. The frequencies of the VRN-1 genes in these derivative varieties were in order of Vrn-Dl（67.1%）〉 Vrn-Bl（19.6%）〉 Vrn-Al（5.3%）, which are similar as the former conclusion that Vrn-D1 is associated with the latest heading time, Vrn-A1 the earliest, and Vrn-B1 intermediate values. The frequencies of Vrn-Al and Vrn-B1 loci in the derivative varieties from winter wheat zones were higher than that from spring winter zones. Based on the wheat breeding history in China and the fact of non-random distribution of Vrn-A1 and Vrn-B1 in the derivative varieties from the four parents, there could be a strong selective effect on VRN-1 genes in different regions where the derivative varieties were cultivated.

关键词： vernalization cornerstone breeding parents of wheat derivative varieties

Identification of QTL for adult plant resistance to stripe rust in bread wheat line C33

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Journal of Integrative Agriculture 2020年第3期19卷 624-631页

作者： LUO Jiang-tao ZHENG Jian-min WAN Hong-shen yang wu-yun LI Shi-zhao PU Zong-jun Crop Research Institute Sichuan Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Breeding in Wheat(Southwest)Ministry of Agriculture and Rural Affairs

Stripe rust,caused by Puccinia striiformis ***,is a serious disease in bread wheat(Triticum aestivum L.).Identification and use of adult plant resistance(APR) resources are important for stripe rust resistance *** wheat line C33 is an exotic germplasm that has shown stable APR to stripe rust for more than 10 years in Sichuan Province of ***,183 recombinant inbred lines(RILs) derived from the cross between C33 and a susceptible line X440 were genotyped with diversity arrays technology(DArT) markers to identify resistance quantitative trait locus(QTL).Field trials were conducted in five years at Chengdu and Xindu of Sichuan Province,using maximum disease severity(MDS) as stripe rust reaction phenotypes.A total of four quantitative trait loci(QTLs) were detected,respectively designed as ***-3 AS,***-5 AL,***-5 BL,and ***-7 DS,explaining 4.14-15.21% of the phenotypic ***-5 BL and ***-7 DS were contributed by ***,the level for stripe rust resistance contributed by them was not strong as C33,suggesting the presence of other unidentified QTLs in ***-7 DS corresponded to Yr18 and ***-5 BL remains to be formally *** RIL lines carrying combinations ***-5 AL,***-5 BL,and ***-7 DS showed comparability resistance with *** present study provides resources to pyramid diverse genes into locally adapted elite germplasm to improve the stripe rust resistance of bread wheat.

关键词： APR DArT QTL mapping Triticum aestivum L.

Effect of HMW-GS 6 + 8 and 1.5 + 10 from Synthetic Hexaploid Wheat on Wheat Quality Traits

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Agricultural Sciences in China 2008年第10期7卷 1161-1171页

作者： TANG Yong-lu yang wu-yun TIAN Ji-chun LI Jun CHEN Fang College of Life Sciences Sichuan University Chengdu 610064 P.R.China Crop Research Institute Sichuan Academy of Agricultural Sciences Chengdu 610066 P.R.China Agricultural College Shandong Agricultural University Tai＇an 250100 P.R.China

To determine the effect of 6 ＋ 8 and 1.5 ＋ 10 HMW-GS of synthetic hexaploid wheat （SHW） on main quality parameters of wheat, a set of recombinant inbred lines （RILs） derived from the cross between a SHW with N, 6 ＋ 8, 1.5 ＋ 10 HMW-GS and a cultivar Chuanyu 12-1 （CY 12-1） with 1, 7 ＋ 8, 2 ＋ 12 were planted in three environments in 2005 and 2006 and totally 16 quality parameters were tested for each line. Significant differences in all tested quality parameters but flour yield were observed between the two parents. The mean values of the RILs were intermediate to the parents for grain and protein parameters and some farinograph parameters, flour water absorption （FWA）, and farinograph softening （SOF） but beyond parents at dough stability time （DST）, breakdown time （BRT）, quality number （QN）, noodle score （NS）, and loaf volume （LOV）. All of the quality traits, especially in grain hardness （GH）, zeleny sedimentation volume （SED）, and most of farinograph parameters had significant difference between the different HMW-GS components. The effects of different alleles of HMW-GS at same locus （Glu-A1 or Glu-B1 or GIu-D1） on the different quality parameters were also different and affected by the other two loci. For most of parameters tested, 6 ＋ 8 was better than 7 ＋ 8 and there was no difference between 1.5 ＋ 10 and 2 ＋ 12. End-use quality was greatly influenced by components of HMW-GS. The components of 1, 6 ＋ 8, 1.5 ＋ 10 had the highest LOV and bread score （BS） values, whereas the components of 1, 7＋ 8 and 1.5 ＋ 10 had the highest NS values. Noodle score performed a positive linear relationship with falling number （FN） and its relationships to other quality parameters were affected by environments. Loaf volume had a significant negative relationship to SOF and positive associations with most of quality parameters. It could be concluded that HMW-GS 6＋ 8 from SHW had better overall quality characteristics than 7 ＋ 8, whereas the effects of 1.5 ＋ 10 on quality was different i

关键词： synthetic hexaploid wheat recombinant inbred lines 6 + 8 1.5 + 10 HMW-GS HMW-GS component quality traits

Molecular mapping of YrTZ2,a stripe rust resistance gene in wild emmer accession TZ-2 and its comparative analyses with Aegilops tauschii

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Journal of Integrative Agriculture 2018年第6期17卷 1267-1275页

作者： WANG Zhen-zhong XIE Jing-zhong GUO Li ZHANG De-yun LI Gen-qiao FANG Ti-lin CHEN Yong-xing LI Jun wu Qiu-hong LU Ping LI Miao-miao wu Hai-bin ZHANG Huai-zhi ZHANG Yan yang wu-yun LUO Ming-cheng Fahima Tzion LIU Zhi-yong China Rural Technology Development Center Beijing 100045P.R.China Institute of Genetics and Developmental Biology Chinese Academy of SciencesBeijing 100101P.R.China College of Agronomy and Biotechnology China Agricultural UniversityBeijing 100193P.R.China Department of Plant and Soil Sciences Oklahoma State UniversityStillwaterOK 74078USA Crop Research Institute Sichuan Academy of Agriculture SciencesChengdu 610066P.R.China China National Seed Group Co. Ltd.Beijing 100045P.R.China Department of Plant Sciences University of CaliforniaDavisCA 95616USA Institute of Evolution University of HaifaMount CarmelHaifa 31905Israel

Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici （Pst）, is a devastating disease that can cause severe yield losses. Identification and utilization of stripe rust resistance genes are essential for effective breeding against the disease. Wild emmer accession TZ-2, originally collected from Mount Hermon, Israel, confers near-immunity resistance against several prevailing Pst races in China. A set of 200 F6：7 recombinant inbred lines （RILs） derived from a cross between susceptible durum wheat cultivar Langdon and TZ-2 was used for stripe rust evaluation. Genetic analysis indicated that the stripe rust resistance of TZ-2 to Pst race CYR34 was controlled by a single dominant gene, temporarily designated YrTZ2. Through bulked segregant analysis （BSA） with SSR markers, YrTZ2 was located on chromosome arm 1BS flanked by Xwmc230 and Xgwm413 with genetic distance of 0.8 cM （distal） and 0.3 cM （proximal）, respectively. By applying wheat 90K iSelect SNP genotyping assay, 11 polymorphic loci （consisting of 250 SNP markers） closely linked to YrTZ2 were identified. YrTZ2 was further delimited into a 0.8-cM genetic interval between SNP marker IWB19368 and SSR marker Xgwm413, and cosegregated with SNP marker IWB28744 （co-segregated with 28 SNP）. Comparative genomics analyses revealed high level of collinearity between the YrTZ2 genomic region and the orthologous region of Aegilops tauschii 1DS. The genomic region between loci IWB19368 and IWB31649 harboring YrTZ2 is orthologous to a 24.5-Mb genomic region between AT1D0112 and AT1D0150, spanning 15 contigs on chromosome 1DS. The genetic and comparative maps of YrTZ2 rovide a framework for map-based cloning and marker-assisted selection of YrTZ2.

关键词： Triticum dicoccoides Puccinia striformis f. sp. tritici SNP comparative genomics

Sex-dependent alterations in extracellular vesicles linking chronic spinal cord injury to brain neuroinflammation and neurodegeneration

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Neural Regeneration Research 2025年第2期20卷 483-484页

作者： yun Li Junfang wu Department of Anesthesiology&Shock Trauma and Anesthesiology Research(STAR)CenterUniversity of Maryland School of MedicineBaltimoreMDUSA

Traumatic spinal cord injury(SCI)is a devastating exogenous injury with long-lasting consequences and a leading cause of death and disability *** in assistive technology,rehabilitative interventions,and the ability to identify and intervene in secondary conditions have significantly increased the long-term survival rate of SCI patients,with some people even living well into their seventh or eighth *** survival changes have led neurotrauma researchers to examine how SCI interacts with brain *** health and epidemiological data showed that patients with long-term SCI can have a lower life expectancy and quality of life,along with a higher risk of comorbidities and complications.

关键词： alterations inflammation injury

IoT Empowered Early Warning of Transmission Line Galloping Based on Integrated Optical Fiber Sensing and Weather Forecast Time Series Data

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Computers, Materials & Continua 2025年第1期82卷 1171-1192页

作者： Zhe Li yun Liang Jinyu Wang yang Gao State Grid Henan Electric Power Company Electric Power Scientific Research Institute State Grid Henan Electric Power CompanyZhengzhou450052China

Iced transmission line galloping poses a significant threat to the safety and reliability of power systems,leading directly to line tripping,disconnections,and power *** early warning methods of iced transmission line galloping suffer from issues such as reliance on a single data source,neglect of irregular time series,and lack of attention-based closed-loop feedback,resulting in high rates of missed and false *** address these challenges,we propose an Internet of Things(IoT)empowered early warning method of transmission line galloping that integrates time series data from optical fiber sensing and weather ***,the method applies a primary adaptive weighted fusion to the IoT empowered optical fiber real-time sensing data and weather forecast data,followed by a secondary fusion based on a Back Propagation(BP)neural network,and uses the K-medoids algorithm for clustering the fused ***,an adaptive irregular time series perception adjustment module is introduced into the traditional Gated Recurrent Unit(GRU)network,and closed-loop feedback based on attentionmechanism is employed to update network parameters through gradient feedback of the loss function,enabling closed-loop training and time series data prediction of the GRU network ***,considering various types of prediction data and the duration of icing,an iced transmission line galloping risk coefficient is established,and warnings are categorized based on this ***,using an IoT-driven realistic dataset of iced transmission line galloping,the effectiveness of the proposed method is validated through multi-dimensional simulation scenarios.

关键词： Optical fiber sensing multi-source data fusion early warning of galloping time series data IoT adaptive weighted learning irregular time series perception closed-loop attention mechanism

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《地震勘探爆炸安全规程》应用分析

中国安全生产科学技术 2014年第S1期10卷 328-333页

作者：吴杨云山西省煤炭地质物探测绘院山西晋中030600

通过借鉴矿山爆破被爆岩体的自由面、最小抵抗线、起爆方式、堵塞等技术参数,研究制定了地震勘探爆炸工作中爆炸物品使用标准、起爆方式、炮井距边坡安全距离、炮井堵塞、组合爆炸时起爆网络连接方式、炮井安全深度、起爆时人员失误、... 详细信息

通过借鉴矿山爆破被爆岩体的自由面、最小抵抗线、起爆方式、堵塞等技术参数,研究制定了地震勘探爆炸工作中爆炸物品使用标准、起爆方式、炮井距边坡安全距离、炮井堵塞、组合爆炸时起爆网络连接方式、炮井安全深度、起爆时人员失误、设备故障安全功能设计等七项补充安全技术条件,有效防止了地震勘探生产过程中出现的爆炸飞溅物伤人、充水炮孔冲天水柱触电、山区边坡炮孔炸塌田坎、岩壁等事故,论证了地震勘探爆炸安全规程(GB 12950-91)在实际应用过程中所存在的问题,以及地震勘探爆炸安全生产应具备的技术条件,提出了对《地震勘探爆炸安全规程》进行修订的建议。

关键词：安全规程缺陷研究成果应用价值

Inheritance of Late Maturity α-amylase (LMA) in a Recombinant Inbreed Lines of Chuanmai 42 Crossed with Chuannong 16

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Agricultural Science & Technology 2011年第3期12卷 322-323,329页

作者： yang En-nian ZHANG Yu LI Jun TANG Yong-lu yang wu-yun ZOU Yu-chun Crop Research Institute Sichuan Academy of Agricultural Sciences Chengdu 610066

[Objective] To study the inheritance and possible molecular marker of α-LMA and their relations to falling number. [Method] Recombined inbreed lines (RILS) of Chuanmai 42 (LMA genotype) crossed with Chuannong 16 (no LMA) were employed to test the late maturity α-amylase (LMA) by EL ISA kits,the Falling Number (FN),and three QTL,SSR markers (Xgwm 577,Xwmc276,Xwmc273) of LMA gene. [Result] Results suggested that LMA was controlled by a single,and the three SSR markers seemed to be not efficient to test LMA gene in this RILS. LMA was an important factor on FN,meanwhile FN was also affected by other factors. [Conclusion] The study could provide references for the breed of quality wheat.

关键词： Late maturity α-amylase (LMA) Falling number (FN) SSR s RILS