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MBOAT1 homozygous missense variant causes nonobstructive azoospermia

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Asian Journal of Andrology 2022年第2期24卷 186-190页

作者： yang-yang wan Lan Guo Yao Yao Xiao-Yun Shi Hui Jiang Bo Xu Juan Hua Xian-Sheng Zhang Department of Urology The First Affiliated Hospital of Anhui Medical UniversityHefei 230022China Department of Biochemistry and Molecular Biology School of Basic Medical SciencesAnhui Medical UniversityHefei 230032China The Department of Urology Peking University Third HospitalPeking 100191China Reproductive and Genetic Hospital The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China

Nonobstructive azoospermia(NOA)is a common cause of infertility and is defined as the complete absence of sperm in ejaculation due to defective *** aim of this study was to identify the genetic etiology of NOA in an infertile male from a Chinese consanguineous family.A homozygous missense variant of the membrane-bound O-acyltransferase domain-containing 1(MBOAT1)gene(c.770C>T,***257Met)was found by whole-exome sequencing(WES).Bioinformatic analysis also showed that this variant was a pathogenic variant and that the amino acid residue in MBOAT1 was highly conserved in *** polymerase chain reaction(Q-PCR)analysis showed that the mRNA level of MBOAT1 in the patient was 22.0%lower than that in his ***,we screened variants of MBOAT1 in a broader population and found an additional homozygous variant of the MBOAT1 gene in 123 infertile *** data identified homozygous variants of the MBOAT1 gene associated with male *** study will provide new insights for researchers to understand the molecular mechanisms of male infertility and will help clinicians make accurate diagnoses.

关键词： male infertility membrane-bound O-acyltransferase domain-containing 1 nonobstructive azoospermia whole-exome sequencing

Lymphoplasmacyte-rich meningioma with atypical cystic-solid feature:A case report

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World Journal of Clinical Cases 2020年第18期8卷 4272-4279页

作者： Kang-Chen Gu yang wan Li Xiang Long-Sheng wang Wen-Jun Yao Department of Radiology The Second Affiliated Hospital of Anhui Medical UniversityHefei 230601Anhui ProvinceChina Department of Pathology The Second Affiliated Hospital of Anhui Medical UniversityHefei 230601Anhui ProvinceChina

BACKGROUND Lymphoplasmacyte-rich meningioma(LPRM)is one of the rarest variants of meningioma and is classified as grade I(benign)*** is characterized by abundant infiltrates of lymphocytes and plasma ***,we report an extremely rare case of LPRM with an atypical imaging finding of multiple cysts around a solid *** SUMMARY The patient was a 36-year-old man with intermittent headache,dizziness,and vomiting for 2 *** tomography and magnetic resonance imaging presented a cystic solid mass in the right frontal lobe with heavy peritumoral edema and obvious contrast *** patient was treated with right frontotemporal craniotomy,and gross total resection of the tumor was achieved without adjuvant *** was no clinical or neuroradiological evidence of recurrent or residual tumor for 3 years after initial *** LPRM is one of the rarest variants of ***,the mass of this case had common features,multiple cysts with nonuniform size and thin wall around the solid part are uncommon imaging finding,increasing the rate of *** definitive diagnosis of LPRM relies on histopathological findings.

关键词： Lymphoplasmacyte-rich meningioma Lymphocyte Plasmacyte Computed tomography Magnetic resonance imaging Case report

Recent Advances of DNA Hydrogels in Biomedical Applications

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Journal of Analysis and Testing 2021年第2期5卷 155-164页

作者： Huai-Qing Chen wan yang Hua Zuo Hua-Wei He Ye-Jing wang State Key Laboratory of Silkworm Genome Biology Biological Science Research CenterSouthwest UniversityChongqing 400715China College of Pharmaceutical Sciences Southwest UniversityChongqing 400715China Chongqing Key Laboratory of Sericultural Science Chongqing Engineering and Technology Research Center for Novel Silk MaterialsSouthwest UniversityChongqing 400715China Chongqing Key Laboratory of Soft-Matter Material Chemistry and Function Manufacturing Chongqing 400715China

DNA hydrogel is one of DNA-based nanomaterials with unique advantages such as precise self-assembly,programmability,addressability,high stability,excellent biocompatibility and biodegradability,and tunable *** features have greatly promoted the development of DNA hydrogels in various applications,especially molecular diagnostics,biosensing,drug delivery,and cancer *** this review,we briefly review the history of DNA hydrogels,the latest advances of DNA hydrogels in biomedical applications especially in biosensing,drug delivery and cancer therapy,and prospect the key challenges and future directions.

关键词： DNA hydrogels Self-assembly Biosensing Drug carrier Cancer therapy

BACNN: Multi-scale feature fusion-based bilinear attention convolutional neural network for wood NIR classification

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Journal of Forestry Research 2024年第4期35卷 202-214页

作者： Zihao wan Hong yang Jipan Xu Hongbo Mu Dawei Qi College of Science Northeast Forestry UniversityHarbin 150040People’s Republic of China

Effective development and utilization of wood resources is *** modification research has become an integral dimension of wood science research,however,the similarities between modified wood and original wood render it challenging for accurate identification and classification using conventional image classification ***,the development of efficient and accurate wood classification techniques is *** paper presents a one-dimensional,convolutional neural network(i.e.,BACNN)that combines near-infrared spectroscopy and deep learning techniques to classify poplar,tung,and balsa woods,and PVA,nano-silica-sol and PVA-nano silica sol modified woods of *** results show that BACNN achieves an accuracy of 99.3%on the test set,higher than the 52.9%of the BP neural network and 98.7%of Support Vector Machine compared with traditional machine learning methods and deep learning based methods;it is also higher than the 97.6%of LeNet,98.7%of AlexNet and 99.1%of ***,the classification method proposed offers potential applications in wood classification,especially with homogeneous modified wood,and it also provides a basis for subsequent wood properties studies.

关键词： Wood classification Near infrared spectroscopy Bilinear network SE module Anti-noise algorithm

片仔癀对情志内热诱导流感病毒易感性的作用研究

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中国药理学通报 2024年第8期40卷 1565-1572页

作者：王容陈新星黄睿婷孙万阳何蓉蓉李怡芳黄丰云南中医药大学中药学院云南昆明650500 暨南大学广东省疾病易感性及中医药研发工程技术研究中心广东广州510632 暨南大学中医药与大健康广东省工程研究中心广东广州510632 暨南大学中药及天然药物研究所广东广州510632 暨南大学广东省中药药效物质基础及创新药物研究重点实验室广东广州510632 暨南大学附属江门中医院中国江门529099

目的本研究在中医情志致病理论指导下,基于课题组前期利用小鼠拘束应激附加H1N1感染构建情志内热诱导流感病毒易感模型,评价了名优清热中成药片仔癀对流感病毒易感性的作用。方法小鼠拘束应激18 h后滴鼻感染H1N1流感病毒,记录小鼠的体... 详细信息

目的本研究在中医情志致病理论指导下,基于课题组前期利用小鼠拘束应激附加H1N1感染构建情志内热诱导流感病毒易感模型,评价了名优清热中成药片仔癀对流感病毒易感性的作用。方法小鼠拘束应激18 h后滴鼻感染H1N1流感病毒,记录小鼠的体征和体质量变化,统计各组小鼠的发病率。在病毒感染后的第4日取肺脏组织采用HE染色、免疫组化、Western blot、ELISA等方法检测病理情况和炎症水平;分别通过Western blot和试剂盒检测脂质过氧化终产物水平;利用氧化磷脂组学检测肺组织中各类氧化磷脂的含量。结果片仔癀能够显著降低情志应激小鼠感染流感病毒的发病率,抑制肺组织流感病毒复制与炎症因子释放,改善肺部炎症,缓解脂质过氧化终产物堆积。氧化磷脂组学结果显示,情志应激附加流感感染小鼠肺组织中氧化磷脂酰胆碱和氧化磷脂酰乙醇胺水平均明显增加,片仔癀给药能显著降低磷脂过氧化水平。结论清热中药片仔癀对情志内热诱导的流感病毒易感有良好的改善作用,作用机制可能与调节磷脂过氧化有关。

关键词：情志应激片仔癀清热中药病毒性肺炎氧化应激脂质过氧化

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Acyclic Edge Coloring of 1-planar Graphs without 4-cycles

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Acta Mathematicae Applicatae Sinica 2024年第1期40卷 35-44页

作者： Wei-fan wang Yi-qiao wang wan-shun yang Department of Mathematics Zhejiang Normal UniversityJinhua321004China School of Management Beijing University of Chinese MedicineBeijing100029China School of Mathematics and Information Science Weifang UniversityWeifang261061China

An acyclic edge coloring of a graph G is a proper edge coloring such that there are no bichromatic cycles in *** acyclic chromatic index χ'α(G) of G is the smallest k such that G has an acyclic edge coloring using k *** was conjectured that every simple graph G with maximum degree Δ has χ'_α(G) ≤Δ+2.A1-planar graph is a graph that can be drawn in the plane so that each edge is crossed by at most one other *** this paper,we show that every 1-planar graph G without 4-cycles has χ'_α(G)≤Δ+22.

关键词： 1-planar graph acyclic edge coloring acyclic chromatic index

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百合鳞茎腐烂病菌鉴定及种质资源抗性评价

南方农业学报 2024年第7期55卷 2148-2160页

作者：陈俊吉杨琬芸卢芳连李雪娴李成丽吴红芝云南农业大学园林园艺学院云南昆明650201

【目的】鉴定云南红河地区百合鳞茎腐烂病病原菌并评价百合种质资源抗性,为百合优质生产及抗性品种选育提供参考依据。【方法】收集引起云南百合鳞茎腐烂病样,进行病原菌分离、培养后,通过光学显微镜观察孢子形态及产孢细胞形态和大小,... 详细信息

【目的】鉴定云南红河地区百合鳞茎腐烂病病原菌并评价百合种质资源抗性,为百合优质生产及抗性品种选育提供参考依据。【方法】收集引起云南百合鳞茎腐烂病样,进行病原菌分离、培养后,通过光学显微镜观察孢子形态及产孢细胞形态和大小,并基于百合鳞茎腐烂病病原菌ITS和TEF-1α序列相似性构建系统发育进化树,鉴定引起红河地区切花百合鳞茎腐烂病的病原种类。以梯伯百合鳞茎和橙影百合叶片为试验材料,筛选最优百合鳞茎腐烂病接种方式;评价156份百合种质对层出镰刀菌(Fusarium proliferatum)的抗性强弱并确定抗性等级。【结果】在云南红河地区切花百合鳞茎腐烂病样中,通过分离、纯化培养后获得15株形态相异的疑似百合鳞茎腐烂病病原菌,其中11号病样致病力最强;光学显微镜下,11号菌株的病原菌小孢子数量多,无镰刀形大孢子。系统发育进化树结果显示,11号菌株与MW015680.1:2-519层出镰刀菌LC13659等同属层出镰刀菌的序列相似性达98%以上,11号菌株分别与*** strain MW016480.1:2-519和*** strain MK414217.1:20-677聚为一支,因此将11号菌株鉴定为层出镰刀菌。使用百合鳞片菌饼有伤接种方法的病情等级最高。156份百合种质对层出镰刀菌的抗性评价结果显示,经鳞片接种后,病情指数(DI)最小的是栽培品种Fredo,为18.33%;以Arbatax、Pretty Woman等为代表的69份百合资源为中抗(MR);其中DI最高的种质是野百合1(保山)和滇百合(丽江),达到96.67%。【结论】导致云南红河地区百合鳞茎腐烂病主要病原菌为层出镰刀菌,鳞片有伤接种是百合抗性鉴定适用的方法,筛选出1份高抗百合层出镰刀菌种质Fredo可作为抗性育种材料。

关键词：百合鳞茎腐烂病层出镰刀菌鉴定方法抗病性

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SENEX gene promotes cell proliferation by activating RB/E2F pathway in diffuse large B-cell lymphoma cells

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BIOCELL 2021年第4期45卷 933-942页

作者： JIYU wanG YING PAN yang wan ZHIXIANG wanYAN ZHITAO wanG QIANSHAN TAO ZHIMIN ZHAI Department of Hematology The Second Hospital of Anhui Medical UniversityHefei230601China

The present study aimed to clarify the role of SENEX in malignant cell proliferation in diffuse large B-cell lymphoma(DLBCL).22 DLBCL patients(6 newly diagnosed cases,7 cases at complete remission,and 9 relapsed cases)were included in the *** results indicated that both SENEX gene and protein were significantly increased in peripheral blood mononuclear cells(PBMCs)and tumor cells of relapsed DLBCL patients,accompanied by overexpression of p21,p16,and phosphorylated retinoblastoma(Rb).Silencing the SENEX gene in a DLBCL cell line caused a significant decrease in cell proliferation and inhibited cell cycle progression in the G1 *** Rb and E2F1 were also decreased,and activation of the Rb/E2F1 pathway was obviously *** conclude,the SENEX gene promotes proliferation in PBMC and tumor cells of DLBCL patients by activating the Rb/E2F1 pathway,in a manner suggesting that increased SENEX expression affects the relapse of DLBCL and may serve as an important target for DLBCL therapy.

关键词： SENEX DLBCL Cell proliferation Rb E2F

Does exposure to poultry and wild fowl confer immunity to H5N1 ？

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Chinese Medical Journal 2014年第18期127卷 3335-3343页

作者： wan yang Jeffrey Shaman Department of Environmental Health Sciences Mailman School ofPublic Health Columbia University New York NY 10032 USA

Human outbreaks of highly pathogenic avian influenza （HPAI） such as H5N1 and novel avian strains such as H7N9 have provoked significant public health concern. An outbreak of H5N1 in humans was first reported in Hong Kong （HK）, China in 1997. This event was curtailed by a variety of public health measures including the culling of over 1.5 million chickens in the city.1 HSN1 has since re-emerged in multiple countries with over 600 reported infections and, since 2003, a case fatality of about 59% （World Health Organization （WHO） report, as of January 24, 2014）.

关键词： H5N1 poultry environmental exposure cross-immunity stem antibodies