检索结果-南通市图书馆

Thermal stability of oxygen evolution in photosystem Ⅱ core complex in the presence of digalactosyl diacylglycerol

在线全文

学校读者我要写书评

暂无评论

chinese Science Bulletin 2002年第24期47卷 2089-2092页

作者： yang zhenle, li liangbi & kuang Tingyunphotosynthesis research Center, institute of botany, chinese academy of sciences, beijing 100093. china photosynthesis research Center Institute of Botany Chinese Academy of Sciences Beijing China

The influence of digalactosyldiacylglycerol (DGDG), one of the photosynthetic membrane lipids, on heat inactivation of the process of oxygen evolution has been studied in vitro in photosystem Ⅱ(PS Ⅱ) core complex. It was found that the temperature of semi-inactivation of oxygen evolution in the complex increased from 40.0 to about 43.0℃ in the presence of DGDG with 5-min heat treatment in the dark. Furthermore, when PS Ⅱ core complex was incubated for 5 min at 45.0℃, the oxygen evolution in the complex was completely lost, whilst the DGDG-complexed PS Ⅱ core complex still retained a 16% of activity (100% for 25.0℃). In addition, a 1-h incubation at 38.0℃ inactivated absolutely the oxygen evolution for the PS Ⅱ core complex. By contrast, there remained about 20% of activity (zero time for 100%) for the complex in the presence of DGDG under the same condition. These results indicate a new role of DGDG in the protection of PS Ⅱ core complex against the deleterious effects of temperature. It was

关键词： digalactosyl diacylglycerol photosystem Ⅱ core complex extrinsic protein of 33 kD thermal stability oxygen evolution.

Depletion of phosphatidyl-glycerol head-group induces changes in oxygen evolution and protein secondary structures of photosystem Ⅱ

在线全文

学校读者我要写书评

暂无评论

chinese Science Bulletin 2002年第10期47卷 824-829页

作者： yang zhenle, WANG Zeneng, JIANG Guizhen, xu yinong, li liangbi & kuang Tingyunphotosynthesis research Center, institute of botany, chinese academy of sciences, beijing 100093. china photosynthesis research Center Institute of Botany Chinese Academy of Sciences Beijing China

The techniques of oxygen electrode polarogra-phy and Fourier transform infrared (FT-IR) spectroscopy were employed to explore the roles of polar head-group of phosphatidylglycerol (PG) molecules in the functional and structural aspects of photosystem Ⅱ (PS Ⅱ) through enzymatic approach. It was shown that the depletion of PG by treatment of phospholipase C (PLC) on PS Ⅱ particles caused the inhibition of oxygen evolving activity in PS Ⅱ. This effect also gave rise to changes in the protein secondary structures of PS Ⅱ, that is, an increase in a-helical conformation which is compensated by the loss of p-strand structures. It revealed that the head-group of PG molecules plays an important structural role in the maintenance of normal structure of PS Ⅱ proteins, which is required to maintain the appropriate physiological activity of the PS Ⅱ complex such as the oxygen evolving activity. It is suggested that there most probably exist hydrogen-bonding interactions between PG molecules and PS Ⅱ proteins.

关键词： photosystem II polar head-group of phosphatidylglyc-erol phospholipase C oxygen evolution protein secondary struc-ture.

Increase in electron transfer activity in photosystemⅡof spinach thylakoids caused by conversion of phosphatidyl-glycerol to phosphatidic acid molecules

在线全文

学校读者我要写书评

暂无评论

chinese Science Bulletin 2003年第15期48卷 1581-1585页

作者： WU Feng, yang zhenle, li liangbi & kuang Tingyun Key Laboratory of photosynthesis and Environmental Molecular Physiology & photosynthesis research Center, institute of botany, chinese academy of sciences, beijing 100093. china Correspondence should be addressed to yang zhenle (e-mail: yangzhl @***) Key Lab. of Photosyn. and Environmental Molec. Physiology and photosynthesis research Center Institute of Botany Chinese Academy of Sciences Beijing 100093 China

The techniques of oxygen electrode polarography, sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE) and thin layer chromatography (TLC) were employed to investigate the effect of phospholipase D treatment on physiological function of spinach thylakoids. It was shown that the phospholipase D treatment on thylakoid resulted in the degradation of phosphatidylglycerol (PG) and occurrence of phosphatidic acid (PA). The changes of PG to PA molecules caused an increase in oxygen evolution in photosystem Ⅱ (PSⅡ), which was accompanied by an uncoupling effect on thylakoid membrane. It was revealed that the head-groups of PG molecules play an important role in the maintenance of the appropriate physiological activity of thylakoid membrane.

关键词：电子转移活性类囊体光合作用菠菜磷脂酰甘油

The excitation energy transfer between β-Car and Chla molecules in PS Ⅱ core antenna complexes CP43 and CP47

在线全文

学校读者我要写书评

暂无评论

chinese Science Bulletin 2000年第17期45卷 1579-1583页

作者： SHAN Jixiu, WANG Jushuo, li liangbi,ZHAO Nanming & kuang Tingyun1. Laboratory of photosynthesis basic research, institute of botany, chinese academy of sciences, beijing 100093. china 2. Department of Biological sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, beijing 100084, chinaCorrespondence should be addressed to kuang Tingyun Laboratory of photosynthesis basic research Institute of Botany Chinese Academy of Sciences Beijing China Department of Biological sciences and Biotechnology State Key Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University Beijing China

The excitation energy transfer efficiency between p-Car and Chla molecules in purified CP43 and CP47 was calculated by comparing absorption and fluorescence excitation after normalization at 550 nm, CP43 had an energy transfer efficiency of 29.1% while the CP47 had an energy transfer efficiency of 62.8%, proving that excitation energy was transferred between p-Car and Chla molecules in CP43 and CP47 at normal conditions. The excitation energy transfer between p-Car and Chla molecules in CP43 and CP47 may occur through the "Dexter" mechanism and the distance between these two kinds of pigments should be less than 1 nm. In addition, the results were also used to discuss the conformational relationship between p-Car and Chla molecules in CP43 and CP47.

关键词： CP43 CP47 β-Car Chla excitation energy transfer.

The structural model of P_(680)

在线全文

学校读者我要写书评

暂无评论

chinese Science Bulletin 1999年第9期44卷 862-863页

作者： ZHANG Chunxi , ZHANG Xiaodong , li liangbi , ZHANG Qiyuan , ZHANG Xingkang and kuang Tingyunphotosynthesis research Center and Laboratory of photosynthesis basic research , institute of botany , chinese academy of sciences , beijing 100093. china State Key Laboratory for Structural Chemistry of Unstable and Stable Species , institute of Chemistry, chinese academy of sciences , beijing 100080, china photosynthesis research Center and Laboratory of photosynthesis Hasic research Institute of Botany Chinese Academy of Sciences Beijing China State Key Laboratory for Structural Chemistry of Unstable and Stuble Species. institute of Chemistry Chinese Academy of Sciences Beijing China

INVESTIGATION on photosystem Ⅱ （PS Ⅱ ） of high plants is one of the most important aspects in photosynthesis research. P680 is the primary electron donor in reaction center of PS Ⅱ , which is a crucial component for sun-light conversion and water split in PS Ⅱ. So study on its structure and function is very important. So far, a few structural models of P（680） have been proposed. Typical models are monomolecular model and bimolecular model. There are two types of bimolecular model: bimolecular parallel model and bimolecular angular model. (Note: There is a kind of multiple model. Because it can be obtained from the organization of two bimolecular models, this model is considered as the same sort of bi-

关键词： The structural model of P

The characteristics of CO_2 assimilation of photosynthesis and chlorophyll fluorescence in transgenic PEPC rice

在线全文

学校读者我要写书评

暂无评论

chinese Science Bulletin 2001年第13期46卷 1080-1084页

作者： JIAO Demao, li Xia, HUANG xueqing, CHI Wei, kuang Tingyun & Ku SB Maurice1. institute of Agrobiological Genetics and Physiology, Jiangsu academy of Agricultural sciences, Nangjing 210014, china 2. institute of botany, the chinese academy of sciences, beijing 100093. china 3. botany Department, Washington State University, Pullman, WA 99164-4236, USA institute of Agrobiological Genetics and Physiology Jiangsu Academy of Agricultural Sciences Nangjing China institute of botany The Chinese Academy of Sciences Beijing China botany Department Washington State University Pullman USA

With PEPC, PPDK, NADP-ME and PEPC+ PPDK transgenic and untransformed rice (Orysa sativa L.), the activities of related C4 photosynthesis enzymes, the chlorophyll fluorescence parameters, CO2 exchange and other physiological indexes were compared, in which the physiological characteristics of PEPC transgenic rice were mainly studied. The results were as follows: (i) The activities of PEPC in PEPC transgenic rice were 20-fold higher than those in untransformed rice; the light-saturation photosynthetic rates and the carboxylation efficiency of PEPC transgenic rice were increased by 55% and 50% more than those of untransformed rice, respectively, while the CO2 compensation point decreased by 27%. (ii) The PS II photochemical efficiency (Fv/Fm) and photochemical quenching (qp) of transgenic PEPC rice decreased less in comparison with those of untransformed rice after the treatment with high light intensity (3 h) or methyl viologen (MV), a photooxidative reagent, which demonstrated that the tolerance of PEPC

关键词： transgenic rice photosynthetic characteristics photoin-hibition of photosynthesis chlorophyll fluorescence phosphoenopy-ruvate carboxylase (PEPC).

Positional distribution of fatty acids on the glycerol backbone during the biosyn-thesis of glycerolipids in Ectocarpus fasciculatus

在线全文

学校读者我要写书评

暂无评论

chinese Science Bulletin 2002年第21期47卷 1802-1806页

作者： xu yinong WANG Zeneng VAN Xiaojun liN Wei li liangbi kuang Tingyun Key Laboratory of photosynthesis and Environmental Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China Key Laboratory of Experimental Marine Biology Institute of Marine BiologyChinese Academy of SciencesQingdao 266071China

The biosynthesis of glycolipids in ***-tus was studied by 14C label and chase. The fatty acids in sulphoquinovosyl diacylglycerol (SQDG) were almost 16-car-bon and 18-carbon ones. In addition to the two fatty acids, monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG) contained 8.5 mol% and 31.0 mol% of eicosapentaenoic acid (20 : 5), respectively, and this fatty acid was usually distributed in the sn-1 position of the glyc-erol backbone. When plants were incubated with [2-14C] acetate, differences existed in the positional distribution of the labeled fatty acids in sn-1 and sn-2 among the three glycerolipids. In SQDG, 14C-labeled fatty acids were distributed uniformly in the sn-1 and sn-2 positions. In DGDG, 14C-labeled fatty acids were mainly distributed in the sn-2 position. In MGDG, the radioactivity of fatty acids in sn-1 position was far greater than that in sn-2 position after a 30 min pulse label, and the difference in radioactivity between the two positions

关键词： E. fasciculatus glycerolipid fatty acid isotope label.

Changes in Thermostability of Photosystem Ⅱ and Leaf lipid Composition of Rice Mutant with Deficiency of light-harvesting Chlorophyll a/b Protein Complexes

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2007年第4期49卷 515-522页

作者： Yunlai Tang Mei Chen yinong xu Tingyun kuang College of life Science and Engineering Southwest University of Science and Technology Mianyang 621000 China photosynthesis research Center Institute of Botany the Chinese Academy of Sciences Beijing 100093 China

We studied the difference in thermostability of photosystem Ⅱ （PSII） and leaf lipid composition between a T-DNA insertion mutant rice （Oryza sativa L.） VG28 and its wild type Zhonghuau. Native green gel and SDS-PAGE electrophoreses revealed that the mutant VG28 lacked all light-harvesting chlorophyll a/b protein complexes. Both the mutant and wild type were sensitive to high temperatures, and the maximal efficiency of PSII photochemistry （FJ Fm） and oxygen-evolving activity of PSII in leaves significantly decreased with increasing temperature. However, the PSII activity of the mutant was markedly more sensitive to high temperatures than that of the wild type. lipid composition analysis showed that the mutant had less phosphatidylglycerol and sulfoquinovosyl diacylglycerol compared with the wild type. Fatty acid analysis revealed that the mutant had an obvious decrease in the content of 16：1t and a marked increase in the content of 18：3 compared with the wild type. The effects of lipid composition and unsaturation of membrane lipids on the thermostability of PSII are discussed.

关键词： high temperature lipid composition light-harvesting a/b protein complexes photosystem II rice.

Mutagenesis of Ser^(24) of cytochrome b559 α subunit affects PSII activities in Chlamydomonas reinhardtii

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

chinese Science Bulletin 2007年第7期52卷 896-902页

作者： MA JingJing li liangbi JING YuXiang kuang TingYun Key Laboratory of photosynthesis and Environmental Molecular Physiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China Graduate University of chinese academy of sciences Beijing 100049 China

In order to study the functions of cytochrome b559 (Cyt b559) in photosystem two (PSII) activity, mutant S24F of Chlamydomonas reinhardtii was constructed using site directed mutagenesis, in which Serine24 (Ser24) locating downstream of Histidine23 (His23) in α subunit of Cyt b559 was replaced by Phenylalanine (Phe). Physiological and biochemical analysis showed that mutant S24F could be grown photoautotrophically or photoheterotrophically. However, their growth rate was slower either on HSM or TAP medium than that of the control; Analysis of PSII activity revealed that its oxygen evolution was about 71% of wild type (WT); The Photochemical efficiency of PSII (Fv/Fm) of S24F was reduced 0.23 compared with WT; S24F was more sensitive to strong light irradiance than the wild type; Furthermore, SDS-PAGE and Western-blotting analysis indicated that the expression levels of α subunit of Cyt b559, LHCII and PsbO of S24F were a little less than those of the wild type. Overall, these data suggests that Ser24 plays a significant role in making Cyt b559 structure maintain PSII complex activity of oxygen evolution although it is not directly bound to heme group.

关键词：莱茵衣藻细胞色素b559α亚基丝氨酸残基 Ser24 突变光系统II 光合活性