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Depletion of phosphatidyl-glycerol head-group induces changes in oxygen evolution and protein secondary structures of photosystem Ⅱ

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chinese Science Bulletin 2002年第10期47卷 824-829页

作者： yang zhenle, WANG Zeneng, JIANG Guizhen, xu yinong,{3. & {3. Tingyunphotosynthesis research Center, institute of botany, chinese academy of sciences, Beijing 100093. China photosynthesis research Center Institute of Botany Chinese Academy of Sciences Beijing China

The techniques of oxygen electrode polarogra-phy and Fourier transform infrared (FT-IR) spectroscopy were employed to explore the roles of polar head-group of phosphatidylglycerol (PG) molecules in the functional and structural aspects of photosystem Ⅱ (PS Ⅱ) through enzymatic approach. It was shown that the depletion of PG by treatment of phospholipase C (PLC) on PS Ⅱ particles caused the inhibition of oxygen evolving activity in PS Ⅱ. This effect also gave rise to changes in the protein secondary structures of PS Ⅱ, that is, an increase in a-helical conformation which is compensated by the loss of p-strand structures. It revealed that the head-group of PG molecules plays an important structural role in the maintenance of normal structure of PS Ⅱ proteins, which is required to maintain the appropriate physiological activity of the PS Ⅱ complex such as the oxygen evolving activity. It is suggested that there most probably exist hydrogen-bonding interactions between PG molecules and PS Ⅱ proteins.

关键词： photosystem II polar head-group of phosphatidylglyc-erol phospholipase C oxygen evolution protein secondary struc-ture.

Thermal stability of oxygen evolution in photosystem Ⅱ core complex in the presence of digalactosyl diacylglycerol

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chinese Science Bulletin 2002年第24期47卷 2089-2092页

作者： yang zhenle, li liangbi & kuang Tingyun{3. research Center, institute of botany, chinese academy of sciences, Beijing 100093. China photosynthesis research Center Institute of Botany Chinese Academy of Sciences Beijing China

The influence of digalactosyldiacylglycerol (DGDG), one of the photosynthetic membrane lipids, on heat inactivation of the process of oxygen evolution has been studied in vitro in photosystem Ⅱ(PS Ⅱ) core complex. It was found that the temperature of semi-inactivation of oxygen evolution in the complex increased from 40.0 to about 43.0℃ in the presence of DGDG with 5-min heat treatment in the dark. Furthermore, when PS Ⅱ core complex was incubated for 5 min at 45.0℃, the oxygen evolution in the complex was completely lost, whilst the DGDG-complexed PS Ⅱ core complex still retained a 16% of activity (100% for 25.0℃). In addition, a 1-h incubation at 3..0℃ inactivated absolutely the oxygen evolution for the PS Ⅱ core complex. By contrast, there remained about 20% of activity (zero time for 100%) for the complex in the presence of DGDG under the same condition. These results indicate a new role of DGDG in the protection of PS Ⅱ core complex against the deleterious effects of temperature. It was

关键词： digalactosyl diacylglycerol photosystem Ⅱ core complex extrinsic protein of 3. kD thermal stability oxygen evolution.

Increase in electron transfer activity in photosystemⅡof spinach thylakoids caused by conversion of phosphatidyl-glycerol to phosphatidic acid molecules

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chinese Science Bulletin 2003年第15期48卷 1581-1585页

作者： WU Feng, yang zhenle, li liangbi & kuang Tingyun Key Laboratory of {3. and Environmental Molecular Physiology & {3. research Center, institute of botany, chinese academy of sciences, Beijing 100093. China Correspondence should be addressed to yang zhenle (e-mail: yangzhl @***) Key Lab. of Photosyn. and Environmental Molec. Physiology and photosynthesis research Center Institute of Botany Chinese Academy of Sciences Beijing 100093 China

The techniques of oxygen electrode polarography, sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE) and thin layer chromatography (TLC) were employed to investigate the effect of phospholipase D treatment on physiological function of spinach thylakoids. It was shown that the phospholipase D treatment on thylakoid resulted in the degradation of phosphatidylglycerol (PG) and occurrence of phosphatidic acid (PA). The changes of PG to PA molecules caused an increase in oxygen evolution in photosystem Ⅱ (PSⅡ), which was accompanied by an uncoupling effect on thylakoid membrane. It was revealed that the head-groups of PG molecules play an important role in the maintenance of the appropriate physiological activity of thylakoid membrane.

关键词：电子转移活性类囊体光合作用菠菜磷脂酰甘油

The excitation energy transfer between β-Car and Chla molecules in PS Ⅱ core antenna complexes CP43.and CP47

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chinese Science Bulletin 2000年第17期45卷 1579-1583页

作者： SHAN Jixiu, WANG Jushuo, li liangbi,ZHAO Nanming & kuang Tingyun1. Laboratory of photosynthesis {3. research, institute of botany, chinese academy of sciences, Beijing 100093. China 2. Department of Biological sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, ChinaCorrespondence should be addressed to kuang Tingyun Laboratory of photosynthesis basic research Institute of Botany Chinese Academy of Sciences Beijing China Department of Biological sciences and Biotechnology State Key Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University Beijing China

The excitation energy transfer efficiency between p-Car and Chla molecules in purified CP43.and CP47 was calculated by comparing absorption and fluorescence excitation after normalization at 550 nm, CP43.had an energy transfer efficiency of 29.1% while the CP47 had an energy transfer efficiency of 62.8%, proving that excitation energy was transferred between p-Car and Chla molecules in CP43.and CP47 at normal conditions. The excitation energy transfer between p-Car and Chla molecules in CP43.and CP47 may occur through the "Dexter" mechanism and the distance between these two kinds of pigments should be less than 1 nm. In addition, the results were also used to discuss the conformational relationship between p-Car and Chla molecules in CP43.and CP47.

关键词： CP43 CP47 β-Car Chla excitation energy transfer.

The structural model of P_(680)

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chinese Science Bulletin 1999年第9期44卷 862-863页

作者： ZHANG Chunxi , ZHANG Xiaodong , li liangbi , ZHANG Qiyuan , ZHANG Xingkang and kuang Tingyunphotosynthesis {3. Center and Laboratory of photosynthesis basic {3. , institute of botany , chinese academy of sciences , Beijing 100093. China State Key Laboratory for Structural Chemistry of Unstable and Stable Species , institute of Chemistry, chinese academy of sciences , Beijing 100080, China photosynthesis research Center and Laboratory of photosynthesis Hasic research Institute of Botany Chinese Academy of Sciences Beijing China State Key Laboratory for Structural Chemistry of Unstable and Stuble Species. institute of Chemistry Chinese Academy of Sciences Beijing China

INVESTIGATION on photosystem Ⅱ （PS Ⅱ ） of high plants is one of the most important aspects in photosynthesis research. P680 is the primary electron donor in reaction center of PS Ⅱ , which is a crucial component for sun-light conversion and water split in PS Ⅱ. So study on its structure and function is very important. So far, a few structural models of P（680） have been proposed. Typical models are monomolecular model and bimolecular model. There are two types of bimolecular model: bimolecular parallel model and bimolecular angular model. (Note: There is a kind of multiple model. Because it can be obtained from the organization of two bimolecular models, this model is considered as the same sort of bi-

关键词： The structural model of P

The characteristics of CO_2 assimilation of photosynthesis and chlorophyll fluorescence in transgenic PEPC rice

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chinese Science Bulletin 2001年第13期46卷 1080-1084页

作者： JIAO Demao, li Xia, HUANG xueqing, CHI Wei, kuang Tingyun & Ku SB Maurice1. {3. of Agrobiological Genetics and Physiology, Jiangsu academy of Agricultural {3., Nangjing 210014, China 2. institute of botany, the chinese academy of sciences, Beijing 100093. China 3. botany Department, Washington State University, Pullman, WA 99164-423., USA institute of Agrobiological Genetics and Physiology Jiangsu Academy of Agricultural Sciences Nangjing China institute of botany The Chinese Academy of Sciences Beijing China botany Department Washington State University Pullman USA

With PEPC, PPDK, NADP-ME and PEPC+ PPDK transgenic and untransformed rice (Orysa sativa L.), the activities of related C4 photosynthesis enzymes, the chlorophyll fluorescence parameters, CO2 exchange and other physiological indexes were compared, in which the physiological characteristics of PEPC transgenic rice were mainly studied. The results were as follows: (i) The activities of PEPC in PEPC transgenic rice were 20-fold higher than those in untransformed rice; the light-saturation photosynthetic rates and the carboxylation efficiency of PEPC transgenic rice were increased by 55% and 50% more than those of untransformed rice, respectively, while the CO2 compensation point decreased by 27%. (ii) The PS II photochemical efficiency (Fv/Fm) and photochemical quenching (qp) of transgenic PEPC rice decreased less in comparison with those of untransformed rice after the treatment with high light intensity (3.h) or methyl viologen (MV), a photooxidative reagent, which demonstrated that the tolerance of PEPC

关键词： transgenic rice photosynthetic characteristics photoin-hibition of photosynthesis chlorophyll fluorescence phosphoenopy-ruvate carboxylase (PEPC).

Positional distribution of fatty acids on the glycerol backbone during the biosyn-thesis of glycerolipids in Ectocarpus fasciculatus

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chinese Science Bulletin 2002年第21期47卷 1802-1806页

作者： xu yinong WANG Zeneng VAN Xiaojun liN Wei li liangbi kuang Tingyun Key Laboratory of photosynthesis and Environmental Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China Key Laboratory of Experimental Marine Biology Institute of Marine BiologyChinese Academy of SciencesQingdao 266071China

The biosynthesis of glycolipids in ***-tus was studied by 14C label and chase. The fatty acids in sulphoquinovosyl diacylglycerol (SQDG) were almost 16-car-bon and 18-carbon ones. In addition to the two fatty acids, monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG) contained 8.5 mol% and 3..0 mol% of eicosapentaenoic acid (20 : 5), respectively, and this fatty acid was usually distributed in the sn-1 position of the glyc-erol backbone. When plants were incubated with [2-14C] acetate, differences existed in the positional distribution of the labeled fatty acids in sn-1 and sn-2 among the three glycerolipids. In SQDG, 14C-labeled fatty acids were distributed uniformly in the sn-1 and sn-2 positions. In DGDG, 14C-labeled fatty acids were mainly distributed in the sn-2 position. In MGDG, the radioactivity of fatty acids in sn-1 position was far greater than that in sn-2 position after a 3. min pulse label, and the difference in radioactivity between the two positions

关键词： E. fasciculatus glycerolipid fatty acid isotope label.

A cytochrome c551 mediates the cyclic electron transport chain of the anoxygenic phototrophic bacterium Roseiflexus castenholzii

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Plant Communications 2024年第2期5卷 79-93页

作者： Lu Yu Zhenzhen Min Menghua liu Yueyong Xin Aokun liu Jian kuang Wenping Wu Jingyi Wu Huimin He Jiyu Xin Robert EBlankenship Changlin Tian Xiaoling xu Department of Biochemistry and Molecular Biology School of Basic Medical Sciences and Affiliated HospitalKey Laboratory of Aging and Cancer Biology of Zhejiang ProvinceHangzhou Normal UniversityHangzhou 311121China High Magnetic Field Laboratory Chinese Academy of SciencesHefei 230031China photosynthesis research Center College of Life and Environmental SciencesHangzhou Normal UniversityHangzhou 311121China The First Affiliated Hospital of USTC Division of Life Sciences and MedicineCenter for Bioanalytical ChemistryHefei National Laboratory of Physical Science at MicroscaleUniversity of Science and Technology of ChinaHefei 230026China Departments of Biology and Chemistry Washington University in St.LouisSt.LouisMO 63130USA

Roseiflexus castenholzii is a gram-negativefilamentous phototrophic bacterium that carries out anoxygenic photosynthesis through a cyclic electron transport chain(ETC).The ETC is composed of a reaction center(RC)–light-harvesting(LH)complex(rcRC–LH);an alternative complex III(rcACIII),which functionally re-places the cytochrome bc1/b6f complex;and the periplasmic electron acceptor auracyanin(rcAc).Although compositionally and structurally different from the bc1/b6f complex,rcACIII plays similar essential roles in oxidizing menaquinol and transferring electrons to the ***,rcACIII-mediated electron transfer(which includes both an intraprotein route and a downstream route)has not been clearly elucidated,nor have the details of cyclic ***,we identify a previously unknown monoheme cytochrome c(cyt c551)as a novel periplasmic electron acceptor of *** reduces the light-excited rcRC–LH to complete a cyclic *** also reveal the molecular mechanisms involved in the ETC using electron paramagnetic resonance(EPR),spectroelectrochemistry,and enzymatic and structural *** that electrons released from rcACIII-oxidized menaquinol are transferred to two alternative periplasmic electron acceptors(rcAc and cyt c551),which eventually reduce the rcRC to form the complete cyclic *** work serves as a foundation for further studies of ACIII-mediated electron transfer in anoxygenic photosynthesis and broadens our under-standing of the diversity and molecular evolution of prokaryotic ETCs.

关键词： cytochrome c menaquinol oxidoreductase electron transport chain electron paramagnetic reso-nance light-harvesting complex

Expression of pea antisense Lhcb2 gene in transgenic tobacco and its spectrum analysis

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Expression of pea antisense Lhcb2 gene in transgenic tobacco...