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基于miR-665/DRAM1信号介导的自噬探讨补阳还五汤延缓血管衰老的作用

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南京中医药大学学报 2024年第4期40卷 369-378页

作者：叶才博陈祥宇杜杰勇杨玉彬舒尊鹏张莉广东药科大学附属第一医院广东广州510080 广东药科大学中药学院广东广州510006 北京师范大学文理学院广东珠海519085

目的研究补阳还五汤对血管衰老的延缓作用,探讨其机制是否与microRNA-665(miR-665)/DNA损伤调节自噬调控因子1(DNA damage-regulated autophagy modulator 1,DRAM1)信号介导的自噬有关。方法将自然衰老雄性SD大鼠随机分为衰老组,补阳还... 详细信息

目的研究补阳还五汤对血管衰老的延缓作用,探讨其机制是否与microRNA-665(miR-665)/DNA损伤调节自噬调控因子1(DNA damage-regulated autophagy modulator 1,DRAM1)信号介导的自噬有关。方法将自然衰老雄性SD大鼠随机分为衰老组,补阳还五汤低、中、高(9.25、18.5、37.0 g·kg^(-1))剂量组和白藜芦醇组(80 mg·kg^(-1)),同时设立年轻组。分离胸主动脉,ELISA法测定血管组织衰老相关β-半乳糖苷酶(Senescence associatedβ-galactosidase,SA-β-Gal)活性和晚期糖基化终末产物(Advanced glycation end products,AGEs)水平;HE、Masson和EVG染色观察血管组织形态结构;qPCR检测血管组织miR-665表达;生物信息学分析和双荧光素酶报告基因实验验证miR-665与DRAM1靶向关系;透射电镜观察血管内自噬小体;Western blot法检测血管组织p16、DRAM1蛋白及自噬相关蛋白LC3、Beclin1和p62的表达;免疫组织化学法检测血管组织DRAM1的蛋白表达。结果与年轻组相比,衰老组大鼠血管中SA-β-Gal活性、AGEs水平和p16蛋白表达增加(P<0.01);血管组织排列紊乱,中膜增厚,胶原纤维增加,弹力纤维出现断裂、紊乱;miR-665基因表达上调(P<0.01);自噬小体数量减少,Beclin1和LC3Ⅱ/Ⅰ蛋白表达降低(P<0.01),p62蛋白表达升高(P<0.01);DRAM1蛋白表达降低(P<0.01)。与衰老组相比,补阳还五汤和白藜芦醇干预能够降低衰老大鼠血管中SA-β-Gal活性(P<0.01)、AGEs水平和p16蛋白表达(P<0.05,P<0.01);改善血管形态和弹力纤维结构,降低血管组织胶原纤维含量。高剂量补阳还五汤明显下调miR-665基因表达(P<0.01),增加血管内自噬小体数量;不同剂量补阳还五汤明显上调Beclin1蛋白表达(P<0.05,P<0.01),中剂量和高剂量补阳还五汤显著上调LC3Ⅱ/Ⅰ蛋白表达(P<0.01),下调p62蛋白表达(P<0.01);高剂量补阳还五汤明显上调DRAM1蛋白表达(P<0.05)。生物信息学分析显示,miR-665与DRAM1基因序列存在特异性互补结合位点,双荧光素酶报告实验证实miR-665靶向DRAM1基因并负调控DRAM1蛋白表达。结论补阳还五汤可能通过靶向抑制miR-665促进DRAM1蛋白表达,进而促进血管自噬,延缓血管衰老。

关键词：补阳还五汤 miR-665 DRAM1 自噬转录后调控血管衰老

番禺区托幼机构儿童眼保健视力筛查及防治体系建设探讨

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中国医药科学 2024年第2期14卷 142-145页

作者：张小华古艳芳杨育彬广州市番禺区妇幼保健计划生育服务中心儿童群体保健科广东广州511400 广州市番禺区妇幼保健院儿童群体保健科广东广州511400 广州市番禺区妇幼保健院眼科广东广州511400

目的托幼机构儿童开展眼保健视力筛查,了解托幼机构儿童视力筛查异常发生率,调查视力异常诊治情况,建立适当的托幼机构儿童眼保健视力筛查及防治体系。方法抽取番禺区2022年1—12月10间幼儿园共4308名托幼机构儿童视力筛查数据,并进行... 详细信息

目的托幼机构儿童开展眼保健视力筛查,了解托幼机构儿童视力筛查异常发生率,调查视力异常诊治情况,建立适当的托幼机构儿童眼保健视力筛查及防治体系。方法抽取番禺区2022年1—12月10间幼儿园共4308名托幼机构儿童视力筛查数据,并进行回顾性分析,追踪所有视力筛查初筛结果异常儿童的复查和诊治情况。结果在4308名托幼机构儿童中,视力筛查初筛结果未见异常4010名,轻度异常179名,异常119名,异常率为6.92%。初筛结果为轻度异常和异常的共298名儿童中,追踪到去医疗机构复诊的有278名,复查率为93.29%。复查结果为正常的25名,复查结果为异常的有253名,已经配镜的有110名儿童。提示儿童的视力异常情况呈现低龄化。结论番禺区托幼机构儿童眼保健视力筛查结果中视力筛查结果异常儿童占一定比例,情况不容忽视,早期开展视力筛查可以及时发现视力异常儿童,通过及时有效的矫治可以延缓视力异常的发展,有效的宣教和及时的干预可降低托幼机构儿童视力异常发生率和严重率。

关键词：托幼机构儿童眼保健视力筛查防治体系建设

Study on Function of Rare Earth During Producing Phosphate Fertilizer by Wet Process

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Journal of Rare Earths 2004年第z1期22卷 261-264页

作者： Wang Jiachen Liu Xiangsheng yang Jun Fan yubin Wu Yanping Beijing General Research Instiutes for Nonferrous Metals 100088 BeijingChina National Rare Earth Center for Agriculture of China100088 BeijingChina Grirem Advanced Materials Co. Ltd. Beijing 100088China Beijing General Research Instiutes for Nonferrous Metals100088 BeijingChina National Rare Earth Center for Agriculture of China100088 BeijingChina Grirem Advanced Materials Co. Ltd. Beijing 100088China

The impacts of the Rare Earth (RE) on the contents of available Phosphorus (P) and the physical character of the calcium superphosphate produced by the wet process on the basis of simulating the consumption of concentrated sulphuric acid, the slurry concentration and the cure conditions in different wet process were studied in laboratory, and the results show that adding RE into different wet process does not raise available P, but changes its physical character,for example, the composite is loose and the color is offwhite.

关键词： rare earth phosphate fertilizer, wet process, physical characteristics, available P

Effects of modified Lingguizhugan decoction combined with weekend fasting on metabolic syndrome

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Journal of Traditional Chinese Medicine 2014年第1期34卷 48-51页

作者： yubin yang Qiong Li Shuqing Chen Bin Ke Yinjuan Huang Jian Qin Department of Traditional Chinese Medi- cine First Affiliated Hospital of Sun Yat-Sen University

OBJECTIVE:To assess the therapeutic effect of weekend fasting and administration of a modified Lingguizhugan decoction on metabolic syndrome(MetS).METHODS:Twenty-one patients with MetS were recruited from the First Affiliated Hospital of Sun Yat-Sen *** plasma glucose(FPG),30-min and 2-h post-prandial blood glucose(PG),fasting serum insulin(FINS),blood pressure(BP),body mass index(BMI),waist circumference(WC),homeostasis model assessment for insulin resistance index(HOMA-IR),and levels of triglyceride(TG),total cholesterol(TC),low-density lipoprotein-cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C)were *** were allowed to drink only water and a Chinese herbal decoction during *** samples were tested again after 12 weeks of ***:FPG,30-min PG,2-h PG,FINS,LDL-C,systolic BP,diastolic BP,BMI,WC,and HOMA-IR de-creased significantly(P<0.05)compared with before *** of TG,TC,and HDL-C did not change ***:Weekend fasting improved glucose metabolism,lowered BP,reduced LDL-C levels,BMI,and *** data suggest that weekend fasting may be an effective therapy for MetS by protection against coronary atherosclerosis.

关键词： Fasting Metabolic syndrome X Blood glucose Lingguizhugan decoction

Effect of Linguizhugan decoction on hyperlipidemia rats with intermittent fasting

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Journal of Traditional Chinese Medicine 2013年第2期33卷 250-252页

作者： yubin yang Jian Qin Bin Ke Junjie Zhang Lanying Shi Qiong Li Traditional Chinese Medicine Department First Affiliated Hospital of Sun Yat-Sen University

OBJECTIVE:To explore the effect of Linguizhugan decoction on hyperlipidemia rats with caloric ***:The hyperlipidemia model of rat was induced by high fat diet for 8 *** the model was established,26 rats were randomly divided into 4 groups:the control group(n=6),the model group(n=6),the intermittent fasting(IF) group(n= 8),and the IF and herbal medicine(IFH) group(n= 6).IF group was applied intermittent fasting every other *** IFH group was given Linguizhugan decoction every day and intermittent fasting every other *** samples were taken at the end of 16 weeks,and serum ghrelin and lipid was ***:Serum ghrelin in the IF group significantly increased(P<0.01).Serum ghrelin in IFH group was lower than the IF group(P<0.05),but higher than the model group(P<0.01).CONCLUSION:Linguizhugan decoction may play a part in regulation of energy and appetite in hyperlipidemia rats with IF.

关键词： Hyperlipidemias Fasting Ghrelin Lin-guizhugan decoction

Velocity transformation for compressible wall-bounded turbulence——An approach through the mixing length hypothesis

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Science China(Physics,Mechanics & Astronomy) 2024年第9期67卷 108-125页

作者： Xuke Zhu yubin Song Xiaoshuo yang Zhenhua Xia State Key Laboratory of Fluid Power and Mechatronic Systems and Department of Engineering Mechanics Zhejiang UniversityHangzhou 310027China

In an endeavor to establish a connection between the mean velocity profile in compressible wall-bounded turbulence and its incompressible analogue,a refined version of the Trettel and Larsson's(TL)transformation is systematically derived and rigorously assessed across diverse flow *** the recently proposed intrinsic compressibility effects and modeling the multi-layer structure of mixing lengths,the proposed transformation demonstrates exceptional performance in collapsing 57canonical flow cases,including cooled channel and pipe flows,channel flows with pseudo heat sources,as well as adiabatic and diabatic boundary layer ***,the transformation seamlessly extends to low Reynolds number cooled channel and pipe flows,achieving a level of accuracy unparalleled by other transformations in the current state-of-the-art.

关键词： mixing length hypothesis compressible wall-bounded turbulence velocity transformation law of the wall

Enhanced boron neutron capture therapy(BNCT)through controlled drug release via boron-loaded nanofiber mats

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Nano Research 2024年第8期17卷 7479-7492页

作者： Wenyong Huang Yongjin yang Yong Pan Shiwei Jing Yanxin Qi yubin Huang Faculty of Chemistry Northeast Normal UniversityChangchun 130024China Key Laboratory of Sustainable Advanced Functional Materials of Jilin Province Changchun 130024China School of Physics Northeast Normal UniversityChangchun 130024China

Boron neutron capture therapy(BNCT)is a novel binary therapy combining boron targeted drugs and neutron irradiation,which can selectively and effectively kill cancer cells at the cellular *** release of boron drug and its accumulation in tumor sites are the crux of ***,we developed a^(10)B-boric acid(^(10)BA)-loaded nanofiber applying for BNCT by in situ *** nanofibers were obtained by electrospinning technique using polyethylene glycol/polylactide(PEO/PLA)block *** changing the ratio of hydrophilicity to hydrophobicity of the nanofibers,the controlled release and the effective accumulation of boron 10 isotope(^(10)B)were achieved in ***^(10)B content in tumor could reach to 2540μg/g,significantly exceeding the required level of 20–50μg/g for BNCT *** pertinent DNA damage experiments,direct evidence and quantified data of BNCT-induced DNA damage in tumor cells were obtained for the first *** sequencing was employed to predict the molecular mechanisms and potential signaling pathways of BNCT,providing theoretical basis for future combined *** antitumor efficiency of BNCT was demonstrated by establishing mice model of subcutaneous tumor and tumor *** research presents a novel boron-loaded nanofiber mats for BNCT,which enables controlled drug release and holds significant potential in the treatment of unresectable or postoperative residual tumors.

关键词： boron neutron capture therapy nanofibers controlled release in situ administration

Progress on immuno-microenvironment and immune-related therapies in patients with pseudomyxoma peritonei

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Cancer Biology & Medicine 2024年第7期21卷 586-605页

作者： Qidi Zhao Tian Wei Ru Ma yubin Fu Rui yang Yandong Su yang Yu Bing Li Yan Li School of Clinical Medicine Tsinghua UniversityBeijing 100084China Department of Surgical Oncology Beijing Tsinghua Changgung HospitalSchool of Clinical MedicineTsinghua UniversityBeijing 102218China Department of Peritoneal Cancer Surgery Bejing Shijitan HospitalCapital Medical UniversityBejing 100038China

Pseudomyxoma peritonei(PMP) is an indolent malignant syndrome. The standard treatment for PMP is cytoreductive surgery combined with intraperitoneal hyperthermic chemotherapy(CRS + HIPEC). However, the high recurrence rate and latent clinical symptoms and signs are major obstacles to further improving clinical outcomes. Moreover, patients in advanced stages receive little benefit from CRS + HIPEC due to widespread intraperitoneal metastases. Another challenge in PMP treatment involves the progressive sclerosis of PMP cell-secreted mucus, which is often increased due to activating mutations in the gene coding for guanine nucleotide-binding protein alpha subunit(GNAS). Consequently, the development of other PMP therapies is urgently needed. Several immune-related therapies have shown promise, including the use of bacterium-derived non-specific immunogenic agents, radioimmunotherapeutic agents, and tumor cell-derived neoantigens, but a well-recognized immunotherapy has not been established. In this review the roles of GNAS mutations in the promotion of mucin secretion and disease development are discussed. In addition, the immunologic features of the PMP microenvironment and immune-associated treatments are discussed to summarize the current understanding of key features of the disease and to facilitate the development of immunotherapies.

关键词： Pseudomyxoma peritonei tumor immuno-microenvironment immune-related therapy mucin 2 GNAS mutation

High Performance Hydroxide Zinc Carbonate Composite for Supercapacitors

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Journal of Materials Science and Chemical Engineering 2019年第11期7卷 54-60页

作者： Yu Zhao yubin yang Ruiming Ren Liaoning Provincial Key Laboratory of New Energy Battery Dalian Jiaotong University Dalian China

Energy density and production cost for high-performance electrode materials are the main challenge for the capacitive storage technology. In this paper, novel Hydroxide Zinc Carbonate (Zn4CO3(OH)6·H2O, HZC) catalyst layers, which are composed of irregular stagger arrangement nanosheets, have been synthesized successfully on foam Ni from inorganic precursors by a feasible in situ hydrothermal method. Measured by electrochemical tests as electrode materials for supercapacitors, the HZC@Ni foam show high specific capacitance (1329.2 F·g−1 at 1 A·g−1 and 882.8 F·g−1 at 10 A·g−1, respectively). These results show that the HZC@Ni foam could be a potential electrode material for supercapacitors. These encouraging results make these low-cost and eco-friendly materials promising for energy storage application.

关键词： Hydroxide Zinc Carbonate In situ Hydrothermal Method Supercapacitor Specific Capacitance