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Ethanolic extract of Puhuang(Pollen Typhae) modulates lipopolysaccharide-induced inflammatory response through inducible nitric oxide synthase/cyclooxygenase-2 signaling in RAW 264.7 macrophages

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Journal of Traditional Chinese Medicine 2021年第6期41卷 836-844页

作者： CHEN Tzufan CHEN Chuntzu HUANG yuanli BASKARAN Rathinasamy TSAI Jeffrey J.P. HU Rouhmei Department of Biotechnology Asia UniversityTaichung 41354China Taiwan Department of Bioinformatics and Medical Engineering Asia UniversityTaichung 41354China Taiwan

OBJECTIVE:To evaluate the immune modulatory response of Puhuang(Pollen Typhae),ethanolic extract of dried pollens(TP-E)and charcoal activated pollens(CTP-E)were used for their phytochemical evaluation and their modulatory response against lipopolysaccharide(LPS)induced inflammatory activity on RAW264.7 macrophage ***:Biochemical assays were carried out to quantify the 1,1-Diphenyl-2-picrylhydrazyl Radical Scavenging Activity,Reducing Power,Ferrous ion chelating ability and total polyphenol content and ***-toxic dose of the extract(TP-E and CTP-E)was chosen based on 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide *** of TP-E and CTP-E on lipopolysaccharides-induced inducible nitric oxide synthase(iNOS)and cyclooxygenase-2(COX-2)expression was measured by Western blot and quantitative PCR(q RT-PCR).Expression of inflammatory cytokines,such as interleukins(IL-1βand IL-6)and tumor necrosis factorα(TNF-α),was quantified using q ***-activated protein kinase pathway was analyzed using Western ***:Phytochemical analysis revealed that both TP-E and CTP-E have strong antioxidant activities and high flavonoid and phenolic ***-E and CTP-E effectively inhibit the expression of i NOS and COX-2,thereby inhibiting its downstream proinflammatory regulators,the extracellular signal-related kinase-1/2,that decreases the expression of IL-1β,IL-6 and TNF-α.CONCLUSION:Phytochemical constituents present in Typha angustifolia Linn could be used for treating inflammation-related diseases.

关键词： Typha angustifolia lipopolysaccharides inflammation NF-kappa B mitogen-activated protein kinases

Combined gene therapy of endostatin and interleukin 12 with polyvinylpyrrolidone induces a potent antitumor effect on hepatoma

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World Journal of Gastroenterology 2004年第15期10卷 2195-2200页

作者： Pei-yuanli Ju-ShengLin Zuo-HuaFeng Yu-FeiHe He-JunZhou XinMa Xiao-KunCai De-AnTian InstituteofLiverDiseases TongjiHospitalTongjiWuhan430030HubeiProvinceChina DepartmentofBiochemistryandMolecularBiology TongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhan430030HubeiProvinceChina

AIM: To study the antitumor effect of combined gene therapy of endostatin and interleukin 12 (IL-12) with polyvinylpyrrolidone (PVP) on mouse transplanted ***: Mouse endostatin eukaryotic plasmid (pSecES) with a mouse Igk signal sequence inside and mouse IL-12 eukaryotic plasmid (pmIL-22) were transfected into BHK-22cells respectively. Endostatin and IL-22 were assayed by ELISA from the supernant and used to culture endothelial cells and spleen lymphocytes individually. Proliferation of the latter was evaluated by M-I-r. H22 cells were inoculated into the leg musde of mouse, which was injected intratumorallywith pSecES/PVP, pmIL-12/PVP or pSecES+pmIL-12/PVPrepeatedly. Tumor weight, serum endostatin and serumIL-22 were assayed. Tumor infiltrating lymphocytes, tumormicrovessel density and apoptosis of tumor cells were also displayed by HE staining, CD32 staining and ***: Endostatin and IL-12 were secreted after transfection, which could inhibit the proliferation of endothelial cells or promote the proliferation of spleen *** growth was highly inhibited by 92.8% after injection of pSecES+pmIL-i2/PVP accompanied by higher serum endostatin and IL-22, more infiltrating lymphocytes, fewertumor vessels and more apoptosis cells compared with injection of pSecES/PVP, pmIL-i2/PVP or vector/***: Mouse endostatin gene and IL-12 gene can be expressed after intratumoral injection with *** of hepatoma can be inhibited synergisticly,lymphocytes can be activated to infiltrate, and tumor cells are induced to apoptosis. Hepatoma can be highly inhibited or eradiated.

关键词：组合基因白细胞间介素12 聚乙烯吡咯烷酮 IL-12 感应抗癌作用肝细胞癌肿瘤 PVP

Gene expression profiles in intracranial aneurysms

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Neuroscience Bulletin 2014年第1期30卷 99-106页

作者： Lanbing Yu Jinghan Fan Shuo Wang Dong Zhang Rong Wang yuanli Zhao Jizong Zhao Department of Neurosurgery Beijing Tiantan Hospital Capital Medical University

In this study, we extracted total RNA from 15 intracranial aneurysms and 17 superficial temporal artery samples, then performed genome-wide expression profiling using the Affymetrix U133 Plus 2.0 GeneChip. Genes that were differentially expressed between intracranial aneurysms and arterial samples were identified using significance analysis for microarrays, and the expression patterns of three randomly-selected genes were verified by real-time polymerase chain reaction analysis. We identified 3 736 differentially-expressed genes out of the 47 000 assayed transcripts. A total of 179 genes showed a 〉10-fold change in expression between the aneurysms and the arterial samples. Genes involved in the proliferation, migration, and apoptosis of vascular muscle cells, atherosclerosis, extracellular matrix disruption, and inflammatory reactions were associated with the formation of intracranial aneurysms. There were no significant differences in gene expression profile between unruptured and ruptured aneurysms.

关键词： intracranial aneurysms microarray gene expression profiles

Preliminary study on androgen dependence of calcitonin gene-related peptide in rat penis

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Asian Journal of Andrology 2005年第1期7卷 55-59页

作者： Zhou-JunShen Shan-WenChen Ying-LiLu Liao-yuanli Xie-LaiZhou Ming-GuangZhang Zhao-DianChen DepartmentofUrology FirstAffiliatedHospitalSchoolofMedicineZhejiangUniversityHangzhou310003China DepartmentofEndocrinology SirRunRunShawHospitalSchoolofMedicineZhejiangUniversityHangzhou310003China

Aim: To study the androgen dependence of the neurotransmitter, calcitonin gene-related peptide (CGRP) in rat penis. Methods: Forty-four Sprague-Dawley rats were randomly divided into Group A (intact controls), Group B (castrated) and Group C (gavaged with finasteride 4.5 mg·kg^(-1).day^(-1)). Four and ten weeks later respectively, half of rats in each group were anaesthetized. Blood samples were taken for the measurement of serum testosterone and dihydrotestosterone (DHT) by means of radioimmunoassay. Penile samples were harvested for the investigation of calcitonin gene related peptide (CGRP)-immunoreactive nerve fibers with immunohistochemistry. The computer-assisted imaging analysis system was applied to calculate the area proportion of the CGRP-positive nerve fibers (CGRP-PNF) in each group. Results: 1) Both 4 and 10 weeks later, testosterone and DHT levels in Group B decreased significantly compared with those in Group A, (P 0.05); However, 10 weeks later, the proportion of CGRP-PNF in Groups B and C was significantly less than that in Group A (P < 0.01);3) The proportion of CGRP-PNF of 4-week animals in Groups B and C was significantly higher than that of 10-week animals (P < 0.05). Conclusion: The expression of neurotransmitter, CGRP may depend on androgens, including testosterone and DHT in rat penis.

关键词： neurotransmitters calcitonin gene-related peptide androgens penis rats

Imbalance of matrix metalloproteinase-9 and matrix metalloproteinase tissue inhibitor-1 may contribute to hemorrhage in cerebellar arteriovenous malformations

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Neural Regeneration Research 2012年第19期7卷 1513-1519页

作者： Fei Di Tongyan Chen Hongli Li Jizong Zhao Shuo Wang yuanli Zhao Dong Zhang Department of Neurosurgery Beijing Tiantan Hospital Capital Medical University Beijing 100050 China Department of Laboratory Medicine Beijing Tiantan Hospital Capital Medical University Beijing 100050 China Department of Pathology Beijing Tiantan Hospital Capital Medical University Beijing 100050 China

In this study, we determined the expression levels of matrix metalloproteinase-2 and -9 and matrix metalloproteinase tissue inhibitor-1 and -2 in brain tissues and blood plasma of patients undergoing surgery for cerebellar arteriovenous malformations or primary epilepsy （control group）. Immunohistochemistry and enzyme-linked immunosorbent assay revealed that the expression of matrix metalloproteinase-9 and matrix metalloproteinase tissue inhibitor-1 was significantly higher in patients with cerebellar arteriovenous malformations than in patients with primary epilepsy. The ratio of matrix metalloproteinase-9 to matrix metalloproteinase tissue inhibitor-1 was significantly higher in patients with hemorrhagic cerebellar arteriovenous malformations compared with those with non-hemorrhagic malformations. Matrix metalloproteinase-2 and matrix metalloproteinase tissue inhibitor-2 levels were not significantly changed. These findings indicate that an imbalance of matrix metalloproteinase-9 and matrix metalloproteinase tissue inhibitor-I, resulting in a relative overabundance of matrix metalloproteinase-9, might be the underlying mechanism of hemorrhage of cerebellar arteriovenous malformations.

关键词： cerebellar arteriovenous malformations hemorrhage matrix metalloproteinase-2 matrixmetalloproteinase-9 tissue matrix metalloproteinase inhibitor-1 tissue matrix metalloproteinaseinhibitor-2 immunohistochemistry enzyme-linked immunosorbent assay neural regeneration

Quantitative angiographic haemodynamic evaluation of bypasses for complex aneurysms: a preliminary study

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Stroke & Vascular Neurology 2022年第1期7卷 54-61页

作者： Junlin Lu Chao Xue Xulin Hu yuanli Zhao Dong Zhang Xiaolin Chen Ji Zong Zhao Neurosurgery Beijing Tiantan HospitalBeijingPeople's Republic of China Department of Industrial Engineering Tsinghua UniversityBeijingPeople's Republic of China Chengdu Institute of Organic Chemistry Chinese Academy of SciencesChengduPeople's Republic of China University of the Chinese Academy of Sciences BeijingPeople's Republic of China China National Clinical Research Center for Neurological Diseases BeijingPeople's Republic of China Stroke Center Beijing Institute for Brain DisordersBeijingPeople's Republic of China Beijing Key Laboratory of Translational Medicine for Cerebrovascular Disease BeijingPeople's Republic of China Beijing Translational Engineering Enter for 3D Printer in Clinical Neuroscience BeijingPeople's Republic of China

Objective Open microsurgery,often with bypass techniques,is indispensable for complex *** date,it remains unknown whether arterial anatomy or quantitative blood flow measurements can predict insufficient flow-related stroke(IRS).The present study aimed to evaluate the risk factors for IRS in patients treated with open microsurgery with bypass procedures for complex internal carotid artery *** Patients with complex aneurysms undergoing bypass surgery were retrospectively *** recipient/donor flow index(RDFI)was preoperatively evaluated using colour-coding *** was defined as the ratio of the cerebral blood volume of the recipient and donor *** sizes of the recipient and donor arteries were *** recipient/donor diameter index(RDDI)was then *** was defined as the presence of new postoperative neurological deficits and infarction on postoperative CT *** assessed the association between RDFI and other variables and the *** Twenty patients(38±12 years)were *** was observed in 12 patients(60%).Patients with postoperative IRS had a higher RDFI than those without postoperative IRS(p2.3 were more likely to develop IRS(p2.3,rather than RDDI,was significantly associated with postoperative *** preoperative evaluation allows appropriate decisions regarding the treatment strategy for preventing postoperative IRS.

关键词： bypass anatomy aneurysm

Total suprarenal aortic occlusion with cardiac disease: a case series of three cases

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World Journal of Emergency Medicine 2024年第1期15卷 59-61页

作者： yuanli Lei Jiaozhen Chen Qin Chen Jiana Yin Weijia Huang Wenxing Song Shouquan Chen Department of Emergency Medicine the First Affi liated Hospital of Wenzhou Medical UniversityWenzhou 325000China Department of Electrocardiogram Wenzhou People's HospitalWenzhou 325000China

Acute aortic occlusion(AAO)has been very rarely reported in the literature and is a potentially life-threatening emergency.[1-5]AAO mostly occurs in cardiac disease patients,[1-4,6,7]and leads to cardiac complications.[1,2,5,6]Suprarenal aortic occlusion(SAO)is a very rare type of AAO,and infrarenal aortic occlusion(IAO)accounts for 75.8%-97.8%.[1,3,5-7]SAO is different from IAO in some clinical manifestations,treatment strategies,and prognoses.[3,6-8]SAO with cardiac disease will make the condition more complex and could easily cause a delay in diagnosis and portend worse outcomes with amputation and mortality rates.[1,3,6]Early and fast diagnosis and positive revascularization treatment are necessary to prevent cases from becoming fatal.[1-3]Herein,we present a case series of SAO with cardiac disease.

关键词： cardiac occlusion diagnosis

Simultaneous Detection of Three Arboviruses Using a Triplex RT-PCR Enzyme Hybridization Assay

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Virologica Sinica 2012年第3期27卷 179-186页

作者： DanDong Shi—hongFu Li-huaWang ZhiLv Tai-yuanli Guo.dongLiang PreventionofVeterinaryMedicineDepartment CollegeofAgricultureYanbianUniversityYanji13300~China StateKeyLaboratoryforInfectiousDiseasePreventionandControlInstituteforViralDiseaseControlandPrevention ChineseCenterforDiseaseControlandPreventionBeo'ing102206China

Arboviruses represent a serious problem to public health and agriculture worldwide. Fast, accurate identification of the viral agents of arbovirus-associated disease is essential for epidemiological surveillance and laboratory investigation. We developed a cost-effective, rapid, and highly sensitive one-step "triplex RT-PCR enzyme hybridization" assay for simultaneous detections of Japanese Encephallitis virus (JEV, Flaviviridae), Getah virus (GETV, Togaviridae), and Tahyna virus (TAHV, Bunyaviridae) using three pairs of primers to amplify three target sequences in one RT-PCR reaction. The analytical sensitivity of this assay was 1 PFU/mL for JEV, 10 PFU/mL for GETV, and 10 PFU/mL for TAHV. This assay is significantly more rapid and less expensive than the traditional serological detection and single RT-PCR reaction methods. When "triplex RT-PCR enzyme hybridization" was applied to 29 cerebrospinal fluid (CSF) samples that were JEV-positive by normal RT-PCR assay, all samples were strongly positive for JEV, but negative for GETV and TAHV, demonstrating a good sensitivity, specificity, and performance at CSF specimen detection.

关键词： Japanese Encephalitis virus (JEV) Getah Virus (GETV) Tahyna Virus (TAHV) Multiplex RT-PCR Enzyme Hybridization

Electromagnetic Tomography System for Defect Detection of High-Speed Rail Wheel

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Journal of Beijing Institute of Technology 2020年第4期29卷 474-483页

作者： Yu Miao Xianglong Liu Ze Liu yuanli Yue Jianli Wu Jiwei Huo Yong Li School of Electronic Information Engineering Beijing Jiaotong UniversityBeijing 100044China School of Electrical and Information Engineering Zhengzhou University of Light IndustryZhengzhou 450002China

A novel electromagnetic tomography(EMT)system for defect detection of high-speed rail wheel is proposed,which differs from traditional electromagnetic tomography systems in its spatial arrangements of coils.A U-shaped sensor array was designed,and then a simulation model was built with the low frequency electromagnetic simulation *** different algorithms were applied to perform image reconstruction,therefore the defects can be detected from the reconstructed *** on the simulation results,an experimental system was built and image reconstruction were performed with the measured *** reconstructed images obtained both from numerical simulation and experimental system indicated the locations of the defects of the wheel,which verified the feasibility of the EMT system and revealed its good application prospect in the future.

关键词： electromagnetic tomography(EMT) high-speed rail wheel defect detection image reconstruction