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Journal of Integrative Agriculture 2012年第3期11卷 439-445页

作者： LI Shan-shan WANG Jia-qi WEI Hong-yang yang yong-xin BU Deng-pan ZHANG Le-ying ZHOU Ling-yun State Key Laboratory of Animal Nutrition Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193P.R.China

Protein phosphorylation is an important post-translational modification that regulates milk protein structure and *** objective of this study was to analyze the presence of phosphorylated *** milk proteins were first separated by SDS polyacrylamide gel *** in gels digestion and extraction,phosphorylated peptides were enriched by titanium dioxide and identified by ultra performance liquid chromatography coupled with nano electrospray ionization tandem mass *** method ensured the identification of 20 phosphorylated peptides,including 7 phosphorylated forms of α_s1-casein,8 α_s2-casein,and 5 β-*** phosphorylated sites derived from 3 α_s1-caseins,3 α_s2-caseins,and 2 β-caseins were also identified,and localized on residues Ser^61,Ser^63 and Ser^130 in α_s1-casein;Thr^145,Ser^146 and Ser^158 in α_s2-casein;and Ser^50 and Thr^56 in β-*** findings provide valuable information for investigating casein phosphorylation of the bovine milk.

关键词： bovine milk casein phosphorylation mass spectrometry titanium dioxide enrichment

Human RING finger protein ZNF645 is a novel testis-specific E3 ubiquitin ligase

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Asian Journal of Andrology 2010年第5期12卷 658-666页

作者： Yun-Qiang Liu Gang Bai Hao Zhang Dan Su Da-Chang Tao Yuan yang yong-xin Ma Si-Zhong Zhang Department of Medical Genetics State Key Laboratory of Biotherapy West China Hospital West China Medical SchoolSiehuan University Chengdu 610041 China

A large number of testis-specific genes are involved in the complex process of mammalian spermatogenesis. Identification of these genes and their roles is important for understanding the mechanisms underlying spermatogenesis. Here we report on a novel human RING finger protein, ZNF645, which contains a C3HC4 RING finger domain, a C2H2 zinc-finger domain, and a proline-rich region, indicating that it has a structure similar to that of the c-Cbl-like protein Hakai. ZNF645 was exclusively expressed in normal human testicular tissue. Immunohistochemical analysis confirmed that ZNF645 protein was present in spermatocytes, round and elongated spermatids, and Leydig cells. Immunofluorescence staining of mature sperms further showed that the ZNF645 protein was localized over the postacrosomal perinuclear theca region and the entire length of sperm tail. An in vitro ubiquitination assay indicated that the RING finger domain of the ZNF645 protein had E3 ubiquitin ligase activity. Therefore, we suggest that ZNF645 might act as an E3 ubiquitin-protein ligase and play a role in human sperm production and quality control.

关键词： E3 ubiquitin ligase RING finger protein spermatogenesis ZNF645

典型因素对无网格压缩波束形成声源识别的影响

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机械工程学报 2022年第5期58卷 98-107页

作者：杨洋褚志刚杨咏馨重庆大学机械与运载工程学院重庆400044 重庆工业职业技术学院车辆工程学院重庆401120

基于传声器阵列测量和压缩感知理论且将目标声源区域看作连续体处理的无网格压缩波束形成声源识别方法在军事、工业、环境等领域具有良好应用前景。采用蒙特卡罗数值模拟和验证试验探究声源相干性、声源最小分离、噪声干扰和数据快拍数... 详细信息

基于传声器阵列测量和压缩感知理论且将目标声源区域看作连续体处理的无网格压缩波束形成声源识别方法在军事、工业、环境等领域具有良好应用前景。采用蒙特卡罗数值模拟和验证试验探究声源相干性、声源最小分离、噪声干扰和数据快拍数目四个典型因素对声源识别性能的影响,结果表明:该方法适用于任意相干性声源和任意数据快拍数目;高概率获得准确结果的条件是声源足够分离(采用矩形阵列时,单数据快拍下,通常要求声源最小分离不小于1/√AB,A和B分别为矩形阵列的行数和列数)和噪声干扰不过强(单数据快拍下,通常要求信噪比优于15 d B);声源不完全相干时,增多数据快拍降低对声源分离和噪声干扰强度的要求,声源完全相干时,增多数据快拍仅降低对噪声干扰强度的要求。典型因素影响的揭示对无网格压缩波束形成方法的恰当运用及声源识别结果的正确分析具有重要指导意义。

关键词：声源识别无网格压缩波束形成典型因素影响

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高喜马拉雅混合岩特征及其成因

地震地质 2013年第2期35卷 261-277页

作者：杨永鑫杨晓松中国地震局地质研究所地震动力学国家重点实验室北京100029

混合岩是深熔作用的存在标志,对研究中下地壳深熔作用机制、地壳流变以及造山带演化和花岗岩的成因具有重要意义。文中对西藏林芝地区和聂拉木地区的混合岩进行了详细的岩相学、岩石学和地球化学特征的研究。岩相学特征显示,研究区的混... 详细信息

混合岩是深熔作用的存在标志,对研究中下地壳深熔作用机制、地壳流变以及造山带演化和花岗岩的成因具有重要意义。文中对西藏林芝地区和聂拉木地区的混合岩进行了详细的岩相学、岩石学和地球化学特征的研究。岩相学特征显示,研究区的混合岩可划分为浅色体、中色体和暗色体3个基本组成部分。对三者切割分离,分别进行了主量元素和稀土元素的分析。结果表明:浅色体由迁移的熔体结晶形成;中色体可以是未发生熔融的原岩,也可以经由未发生迁移的熔体与熔融残留体反应形成;暗色体是由迁移汇集后的熔体与中色体反应形成。由于聚集的熔体可以为暗色矿物的结晶提供良好的结晶空间和物质来源,因此暗色体多数以窄条带产出于浅色体边缘。浅色体和暗色体通常具有岩浆岩的结构,矿物粒径粗大且分布不具有定向性,这是其区别于中色体的重要特征。浅色体显示出明显Eu正异常,暗示长石大量参与了部分熔融过程,并且初始熔体在近源区的冷凝过程中长石优先结晶。浅色花岗岩的Eu负异常可能与熔体在源区的长石结晶有关。退变质反应有可能使部分熔融反应形成的矿物完全消失,因此不宜将反应矿物存在与否作为发生过脱水熔融的判别标准。

关键词：混合岩深熔作用暗色体浅色体中色体高喜马拉雅结晶岩系

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平板耦合颤振过程中气动能量转换特性

振动与冲击 2013年第10期32卷 55-61页

作者：刘祖军杨詠昕葛耀君华北水利水电学院土木与交通学院郑州450011 同济大学桥梁工程系上海200092

基于分步分析思路对耦合颤振运动方程进行解耦,通过引入考虑阻尼影响的初始运动方程,建立了结构-气流系统颤振能量机理的分析框架。结合平板风洞试验研究了颤振发生过程中系统内主要气动能量的变化规律。研究结果表明在平板耦合颤振过... 详细信息

基于分步分析思路对耦合颤振运动方程进行解耦,通过引入考虑阻尼影响的初始运动方程,建立了结构-气流系统颤振能量机理的分析框架。结合平板风洞试验研究了颤振发生过程中系统内主要气动能量的变化规律。研究结果表明在平板耦合颤振过程中联合气动导数数A1*H3*建立了能量在两个自由度上传递的途径,并且在颤振发生过程中由于扭转振动和竖向振动的相位差逐渐减小,使得该联合气动导数对系统的输能能力不断增强。扭转气动阻尼是系统的主要耗能项,并且耗能能力与导数A2*的数值密切相关而与相位差没有关系。扭转气动刚度在一个振动周期内对系统能量几乎没有贡献,对耦合颤振的影响很小。

关键词：颤振能量气动能量输入和转换特性激励-反馈机制分步分析平板断面

Targeting TrkB–PSD-95 coupling to mitigate neurological disorders

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Neural Regeneration Research 2025年第3期20卷 715-724页

作者： xin yang Yu-Wen Alvin Huang John Marshall Department of Molecular Biology Cell Biology and BiochemistryBrown UniversityProvidenceRIUSA Department of Neurology Warren Alpert Medical School of Brown UniversityProvidenceRIUSA Center for Translational Neuroscience Robert J.and Nancy D.Carney Institute for Brain Science and Brown Institute for Translational ScienceBrown UniversityProvidenceRIUSA

Tropomyosin receptor kinase B(TrkB)signaling plays a pivotal role in dendritic growth and dendritic spine formation to promote learning and *** activity-dependent release of brain-derived neurotrophic factor at synapses binds to pre-or postsynaptic TrkB resulting in the strengthening of synapses,reflected by long-term ***,the association of postsynaptic density protein-95 with TrkB enhances phospholipase Cγ-Ca^(2+)/calmodulin-dependent protein kinaseⅡand phosphatidylinositol 3-kinase-mechanistic target of rapamycin signaling required for long-term *** this review,we discuss TrkB-postsynaptic density protein-95 coupling as a promising strategy to magnify brain-derived neurotrophic factor signaling towards the development of novel therapeutics for specific neurological disorders.A reduction of TrkB signaling has been observed in neurodegenerative disorders,such as Alzheimer's disease and Huntington's disease,and enhancement of postsynaptic density protein-95 association with TrkB signaling could mitigate the observed deficiency of neuronal connectivity in schizophrenia and *** with brain-derived neurotrophic factor is problematic,due to poor pharmacokinetics,low brain penetration,and side effects resulting from activation of the p75 neurotrophin receptor or the truncated TrkB.T1 *** TrkB agonists and antibodies that activate TrkB are being intensively investigated,they cannot distinguish the multiple human TrkB splicing isoforms or cell type-specific *** TrkB–postsynaptic density protein-95 coupling provides an alternative approach to specifically boost TrkB signaling at localized synaptic sites versus global stimulation that risks many adverse side effects.

关键词： Angelman syndrome autism brain-derived neurotrophic factor depression neurodegenerative disorder neurodevelopmental disorder postsynaptic density protein-95 synaptic plasticity TrkB

基于大涡模拟方法的多层动网格技术识别平板气动参数

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振动与冲击 2011年第4期30卷 156-160页

作者：刘祖军葛耀君杨詠昕同济大学桥梁工程系上海200092

从弱耦合的角度出发,对流体计算软件fluent进行二次开发,利用其用户自定义函数(UDF)描述结构的运动状态并结合动网格技术实现流固耦合。在保证动网格运动速度符合空间守恒法则的条件下,针对固体模型在流场中运动受网格尺寸限制且易造成... 详细信息

从弱耦合的角度出发,对流体计算软件fluent进行二次开发,利用其用户自定义函数(UDF)描述结构的运动状态并结合动网格技术实现流固耦合。在保证动网格运动速度符合空间守恒法则的条件下,针对固体模型在流场中运动受网格尺寸限制且易造成网格变形过大导致计算失败的问题提出了多层动网格的解决方法。流体动力计算时考虑湍流的作用,采用大涡模拟方法求解N-S方程。数值模拟了平板做单自由度强迫振动的断面绕流流场,通过最小二乘法拟合气动力时程曲线获得气动导数。仿真结果与通过Theodorsen理论导出的平板气动导数具有良好的一致性。

关键词：多层动网格大涡模拟气动参数 UDF 流固耦合空间守恒法则

Distribution and mapping of temperate savanna in the sandy lands of eastern China

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Science China Earth Sciences 2025年第1期68卷 128-141页

作者： Xiaoya LI Feng WANG Tao DUAN Kaijie yang Bin yang Chunmei WANG xin TIAN Qi LU Institute of Ecological Conservation and Restoration Institute of Desertification Studies Chinese Academy of Forestry Institute of Desertification Institute of Desertification Studies Chinese Academy of Forestry Institute of Great Green Wall Satellite Application Centre for Ecology and Environment MEE Institute of Microelectronics Chinese Academy of Sciences Aerospace Information Research Institute Chinese Academy of Sciences Institute of Forest Resource Information Techniques Chinese Academy of Forestry

Savannas are distributed globally across various *** perform a significant socioeconomic role in regulating net primary productivity and the terrestrial carbon *** to the high spatial heterogeneity induced by the mixed distribution of trees and grasses,current global land-cover classification maps often classify savannas as forests or grasslands Temperate savannas in China cover a vast area,yet their spatial patterns and extent remain poorly *** study developed a novel remote sensing temperate savannas mapping method by integrating very high-resolution （VHR） unmanned aerial vehicle （UAV） and GF-6 satellite imagery based on the vegetation structure characteristics of temperate savannas using the FAO definition of *** and validation datasets were collected from VHR UAV imagery covering the main temperate savannah types with a ground area of 1818 *** fractional woody vegetation cover （FWVC） and fractional herbaceous vegetation cover （FHVC） in temperate savannas were estimated by integrating Classification and Regression Tree and Random Forest *** current distribution of temperate savannas in the Horqin and Otindag Sandy Lands of Eastern China was mapped to a unit area of 0.5 *** root-mean-square error （RMSE） values for the FWVC and FHVC models were 10.69% and 11.67%,and the R2values were 0.66 and 0.63,*** assessment indicated that the new temperate savanna map had high identification accuracy,with a Kappa coefficient of 0.70,overall accuracy of 0.88,and average accuracy of *** savannas in the sandy lands of eastern China were primarily distributed in the northern Horqin Sandy Land the central and eastern regions of the Otindag Sandy Land,and the transition zone from the southern slopes of the Greater Khingan Mountains to the plains,covering a total area of 3.27×10～6 *** main savannah types were elm,oak/maple,and shrub *** savannas were widely distributed in the Otindag Sandy

关键词： Temperate savanna UAV imagery GF-6 Fractional woody vegetation cover Fractional herbaceous vegetation cover Machine learning

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飞行模拟机操纵负荷系统操纵力建模与仿真

电光与控制 2014年第9期21卷 80-84页

作者：杨永欣中国民航大学航空地面特种设备基地天津300300

基于某型民航客机的不可逆式操纵系统,对其飞行模拟机电动操纵负荷系统进行建模。以升降舵操纵系统为例,将参数集中化处理,建立操纵系统数学模型,并将复合控制方法嵌入电动操纵负荷系统。利用某自行研发的飞行仿真实验系统进行仿真实验... 详细信息

基于某型民航客机的不可逆式操纵系统,对其飞行模拟机电动操纵负荷系统进行建模。以升降舵操纵系统为例,将参数集中化处理,建立操纵系统数学模型,并将复合控制方法嵌入电动操纵负荷系统。利用某自行研发的飞行仿真实验系统进行仿真实验,并将结果与波音公司实际试飞曲线对比。结果表明,操纵力误差小于10%,舵偏角误差小于2%,满足CCAR-60对飞行模拟机操纵负荷系统的精度要求。

关键词：飞行模拟机操纵负荷系统等效动态模型法复合控制

上、下组合中央稳定板对于箱梁颤振性能的影响

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中国公路学报 2017年第7期30卷 86-93页

作者：夏锦林杨詠昕葛耀君同济大学土木工程防灾国家重点实验室上海200092

为比较不同高度稳定板单侧和上、下组合设置时断面的颤振性能,并说明设置中央稳定板前后不同攻角下颤振发散机理的改变,针对一特定的单箱断面悬索桥,设计并开展了一系列节段模型风洞试验。首先研究了3个攻角(3°,0°,-3°)下单侧设置稳... 详细信息

为比较不同高度稳定板单侧和上、下组合设置时断面的颤振性能,并说明设置中央稳定板前后不同攻角下颤振发散机理的改变,针对一特定的单箱断面悬索桥,设计并开展了一系列节段模型风洞试验。首先研究了3个攻角(3°,0°,-3°)下单侧设置稳定板对颤振性能的影响,进而同时改变上侧和下侧稳定板的高度,探索对应攻角下断面的抗风效果,从而给出稳定板组合设置时的最优高度取值。结合二维三自由度颤振分析方法(2d-3DOF),分析了最优中央稳定板组合设置断面相比于原始断面颤振发散机理的变化。结果表明:无论是上侧或下侧,单侧设置稳定板断面的颤振临界风速基本呈随着稳定板高度先增大后减小的趋势,合理高度的稳定板能提高颤振临界风速;相比于单侧稳定板,同时设置上、下稳定板对于气动稳定性能的改善效果更佳;二维三自由度分析结果显示,中央稳定板显著改变了颤振发散驱动机理,组合设置稳定板的最优断面和原始断面气动阻尼随风速的变化趋势明显不同。

关键词：桥梁工程中央稳定板风洞试验颤振性能二维三自由度方法箱梁