检索结果-南通市图书馆

Study on the Molecular Variation and PCR Detection of Strawberry mottle virus

维普期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2009年第10期8卷 1203-1209页

作者： yang hong-yi LI Li-li DAI hong-yan ZHANG Zhi-hong College of Life Sciences Northeast Forestry University Harbin 150040 P.R. China College of Horticulture Shenyang Agricultural University Shenyang 110161 P.R. China College of Horticulture Northeast Agricultural University Harbin 150030 P.R.China

Strawberry mottle virus （SMoV） is an important viral pathogen infecting strawberry （Fragaria spp.）. The study was conducted to analyze the characterization of the molecular variation of SMoV and develop the methods for detection of SMoV by nested PCR and transcriptional enhancement techniques. The 3 non-coding region （NCR） and large coat protein （LCP） gene of SMoV genome were amplified by reverse transcription-polymerase chain reaction （RT-PCR）. The specific segments were cloned and sequenced. The characterization of the molecular variation for some isolates of SMoV and phylogenetic analysis were studied. Based on the primers located in the conserved region of genome of SMoV, SMoV could be steadily detected using semi-nested PCR and transcriptional enhancement techniques. Both semi-nested PCR and transcriptional enhancement techniques were considerably more sensitive than the standard RT-PCR. The nucleotide sequences of NCR and partial LCP gene of Chinese isolates were obtained, and sequence analysis of the partial LCP gene of various SMoV isolates showed nucleotide identities ranging from 76.8 to 99.7%. There was a slight tendency for isolates to group according to their geographical origin. All 3 Polish isolates, 4 isolates of 7 Dutch isolates, and 3 isolates of 4 Chinese isolates formed a small separate branch, respectively. Two Germanic isolates had a far relationship with other isolates, and formed a separate clade. A high level of sequence variability was found among SMoV isolates, and the Germanic isolates were likely to a special strain group.

关键词： Strawberry mottle virus RT-PCR semi-nested PCR variation phylogenetic analysis

Enhancement of natural ventilation in rectangular enclosure with two openings by thin fin

在线全文

学校读者我要写书评

暂无评论

Journal of Central South University of Technology 2005年第z1期12卷 256-261页

作者： yang yi DENG Qi-hong School of Energy and Power Engineering Central South University Changsha 410083 China School of Energy and Power Engineering Central South University Changsha 410083 China

A numerical method was used to study the natural ventilation in a rectangular enclosure with two symmetrical openings. In order to improve the natural ventilation efficiency, a fin was introduced into the ***-state heat transfer by laminar natural ventilation in a partially divided rectangular enclosure was investigated by numerically solving equations of mass, momentum and energy. Streamlines and isotherms were produced and heat transfer rate were calculated. A parametric study was carried out using the following parameters: Rayleigh number (1 × 103 - 1 × 106) , dimensionless length (0 - 0.7) and position values (-0. 7 - 0. 7). It is found that the Nusselt number is an increasing function of Rayleigh number. By comparing with no-fin case, it is concluded that fin can effectively enhance the natural ventilation in the enclosure.

关键词： fin length fin position discrete heat sources

Novel 18β-glycyrrhetinic acid amide derivatives show dual-acting capabilities for controlling plant bacterial diseases through ROS-mediated antibacterial efficiency and activating plant defense responses

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2023年第9期22卷 2759-2771页

作者： SONG ying-lian LIU hong-wu yang yi-hong HE Jing-jing yang Bin-xin yang Lin-li ZHOU Xiang LIU Li-wei WANG Pei-yi yang Song National Key Laboratory of Green Pesticide/Key Laboratory of Green Pesticide and Agricultural Bioengineering Ministry of Education/Center for R&D of Fine ChemicalsGuizhou UniversityGuiyang 550025P.R.China

Natural products have long been a crucial source of,or provided inspiration for new agrochemical *** occurring 18β-glycyrrhetinic acid shows broad-spectrum bioactivities and is a potential skeleton for novel drug *** extend the utility of 18β-glycyrrhetinic acid for agricultural uses,a series of novel 18β-glycyrrhetinic acid amide derivatives were prepared and evaluated for their antibacterial ***,compound 5k showed good antibacterial activity in vitro against Xanthomonas oryzae ***(Xoo,EC50=3.64 mg L–1),and excellent protective activity(54.68%)against Xoo in *** 5k induced excessive production and accumulation of reactive oxygen species in the tested pathogens,resulting in damaging the bacterial cell *** interestingly,compound 5k could increase the activities of plant defense enzymes including catalase,superoxide dismutase,peroxidase,and phenylalanine ammonia *** together,these enjoyable results suggested that designed compounds derived from 18β-glycyrrhetinic acid showed potential for controlling intractable plant bacterial diseases by disturbing the balance of the phytopathogen’s redox system and activating the plant defense system.

关键词： 18β-glycyrrhetinic acid antibacterial activities defense enzyme activity reactive oxygen species

BMI-1 activates hepatic stellate cells to promote the epithelialmesenchymal transition of colorectal cancer cells

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2023年第23期29卷 3606-3621页

作者： Zhong-yang Jiang Xi-Mei Ma Xiao-Hui Luan Zhen-Yu Liuyang yi-yang hong Yuan Dai Qing-Hua Dong Guan-Yu Wang Department of General Surgery Sir Run Run Shaw HospitalSchool of MedicineZhejiang UniversityHangzhou 310016Zhejiang ProvinceChina Department of Emergency The Second Affiliated Hospital of Zhejing UniversityHangzhou 310016Zhejiang ProvinceChina Biomedical Research Center Sir Run Run Shaw HospitalSchool of MedicineZhejiang UniversityHangzhou 310016Zhejiang ProvinceChina Key Laboratory of Cancer Prevention and Intervention China National Ministry of EducationHangzhou 310009Zhejiang ProvinceChina

BACKGROUND Activated hepatic stellate cells(aHSCs)are the major source of cancer-associated fibroblasts in the *** the crosstalk between aHSCs and colorectal cancer(CRC)cells supports liver metastasis(LM),the mechanisms are largely *** To explore the role of BMI-1,a polycomb group protein family member,which is highly expressed in LM,and the interaction between aHSCs and CRC cells in promoting CRC liver metastasis(CRLM).METHODS Immunohistochemistry was carried out to examine BMI-1 expression in LM and matched liver specimens of *** expression levels of BMI-1 in mouse liver during CRLM(0,7,14,21,and 28 d)were detected by Western blotting(WB)and the quantitative polymerase chain reaction(qPCR)*** overexpressed BMI-1 in HSCs(LX2)by lentivirus infection and tested the molecular markers of aHSCs by WB,qPCR,and the immunofluorescence *** cells(HCT116 and DLD1)were cultured in HSC-conditioned medium(LX2 NC CM or LX2 BMI-1 CM).CM-induced CRC cell proliferation,migration,epithelial-mesenchymal transition(EMT)phenotype,and transforming growth factor beta(TGF-β)/SMAD pathway changes were investigated in vitro.A mouse subcutaneous xenotransplantation tumor model was established by co-implantation of HSCs(LX2 NC or LX2 BMI-1)and CRC cells to investigate the effects of HSCs on tumor growth and the EMT phenotype in *** Positive of BMI-1 expression in the liver of CRLM patients was 77.8%.The expression level of BMI-1 continued to increase during CRLM in mouse liver ***2 overexpressed BMI-1 was activated,accompanied by increased expression level of alpha smooth muscle actin,fibronectin,TGF-β1,matrix metalloproteinases,and interleukin *** cells cultured in BMI-1 CM exhibited enhanced proliferation and migration ability,EMT phenotype and activation of the TGF-β/SMAD *** addition,the TGF-βR inhibitor SB-505124 diminished the effect of BMI-1 CM on SMAD2/3 phosphorylation in CRC ***,BMI-1 overexpressed LX2 HSCs promot

关键词： BMI-1 Hepatic stellate cells Colorectal cancer Liver metastasis Epithelial-mesenchymal transition

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

复杂应力对结构功率流的影响

上海交通大学学报 2017年第9期51卷 1050-1057页

作者：杨念易宏上海交通大学海洋工程国家重点实验室高新船舶与深海开发装备协同创新中心上海200240

基于Kirchhoff平板理论,利用特定阶模态的正交性推导含复杂应力的结构功率流解析式,用于计算含任意分布应力的结构功率流,并通过数值算例验证了所推导的结构功率流解析式的正确性.同时,利用结构功率流解析式分析某焊接平板的复杂应力对... 详细信息

基于Kirchhoff平板理论,利用特定阶模态的正交性推导含复杂应力的结构功率流解析式,用于计算含任意分布应力的结构功率流,并通过数值算例验证了所推导的结构功率流解析式的正确性.同时,利用结构功率流解析式分析某焊接平板的复杂应力对结构速度场和内力场以及功率流的影响.结果表明,复杂应力对焊接平板功率流的影响比对其速度场和内力场的影响更大.在实际工程的振动预测及控制中,应考虑应力因素的影响.

关键词：结构应力振动功率流

GenerationⅣInternational Forum(GIF) R&D Progress for Sodium Cooled Fast Reactor

在线全文

学校读者我要写书评

暂无评论

GenerationⅣInternational Forum(GIF) R&D Progress for Sodium...

Targeting Therapeutic Windows for Rheumatoid Arthritis Prevention

作者： yang hong-yi

After subscribing the Charter of GIF and submitting the Framework Agreement,China formally signed the Sodium Cooled Fast Reactor System Arrangement in March 2009,which is a important milestone of GIF SFR R&D for our *** this basis,we make a big progress in international cooperation of GIF SFR. After the System Arrangement was signed,it is required that making a R&D plan immediately in according to GIF cooperation framework,for participating the international cooperation of GIF and carrying out the cooperation research on SFR.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Chinese Journal of Integrative Medicine 2024年第9期30卷 842-851页

作者： GAO Kai-xin yang yi-hong LIANG Qi MEI Li-yan LIANG You-bang WANG Mao-jie CHEN Xiu-min HUANG Qing-chun WEN Ze-huai HUANG Run-yue Section of Rheumatology and Immunology Research The Second Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou510120China The Second Clinical Medical College Guangzhou University of Chinese MedicineGuangzhou510405China State Key Laboratory of Dampness Syndrome of Chinese Medicine The Second Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou510120China Guangdong-hong Kong-Macao Joint Lab on Chinese Medicine and Immune Disease Research Guangzhou University of Chinese MedicineGuangzhou510405China Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome Guangzhou510120China Guangdong Provincial Key Laboratory of Chinese Medicine for Prevention and Treatment of Refractory Chronic Diseases Guangzhou510120China The Key Unit of Methodology of Clinical Research The Second Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou510120China

Rheumatoid arthritis(RA)is a worldwide public health *** to delay or prevent the onset of RA have attracted much attention in recent years,and researchers are now exploring various prevention *** present,there is still no unified consensus for RA prevention,but targeting therapeutic windows and implementing interventions for at-risk individuals are extremely *** to the limited number of clinical trials on pharmacologic interventions,further studies are needed to explore and establish optimal intervention regimens and effective measures to prevent progression to *** this review,we introduce the RA disease process and risk factors,and present research on the use of both Western and Chinese medicine from clinical perspectives regarding RA ***,we describe several complete and ongoing clinical studies on the use of Chinese herbal formulae for the prevention of RA.

关键词： rheumatoid arthritis prevention Chinese medicine review

在线全文

学校读者我要写书评

暂无评论

福岛核事故后核电厂安全改进行动分析

原子能科学技术 2014年第3期48卷 486-491页

作者：张琳李文宏杨红义清华大学核能与新能源技术研究院北京100084 环境保护部核二司北京100035 深圳中广核工程设计有限公司广东深圳518031 中国原子能科学研究院快堆研究设计所北京102413

介绍了福岛核事故后世界上主要核电国家相继开展的核电厂安全检查、再评价行动,并得出相应的检查和测试结论。法国、美国和中国等国家分别提出了福岛核事故后改进核电厂安全的建议、要求和行动,并制定了具体工程措施:在极端外部事件的设... 详细信息

介绍了福岛核事故后世界上主要核电国家相继开展的核电厂安全检查、再评价行动,并得出相应的检查和测试结论。法国、美国和中国等国家分别提出了福岛核事故后改进核电厂安全的建议、要求和行动,并制定了具体工程措施:在极端外部事件的设防,严重事故预防和缓解,水、电、通风实体改进,限制严重事故下的放射性释放和应急准备等主要方面开展的安全改进行动,将会提高核电厂的安全水平并提升缓解严重事故的能力。反思福岛核事故,总结福岛核事故对核电安全技术改进的促进作用,对未来核电安全技术的发展进行了展望。

关键词：福岛核事故核电厂核安全改进行动

在线全文

学校读者我要写书评

暂无评论

论苏轼对精神寓居空间的营造与书写

西北师大学报（社会科学版） 2024年第4期61卷 64-72页

作者：杨一泓北京师范大学文学院北京100875

苏轼一生迁转的空间直接触动着他的心境,进而影响其诗文创作。他在感知与体认不同生存环境的基础上,营造与书写自己的精神寓居空间。在京城名利场域,苏轼通过对家乡瑞草桥的回望与童年的游历想象使心灵重返故园;贬谪迁徙过程中,苏轼标... 详细信息

苏轼一生迁转的空间直接触动着他的心境,进而影响其诗文创作。他在感知与体认不同生存环境的基础上,营造与书写自己的精神寓居空间。在京城名利场域,苏轼通过对家乡瑞草桥的回望与童年的游历想象使心灵重返故园;贬谪迁徙过程中,苏轼标记、营造不同的谪居处所并将其体认为亲切的归处;苏轼所遇的山水因承载了他的想象与神游亦具有了空间意蕴,庐山和西湖即被书写为真实可返、不断相逢的人世桃源。苏轼的精神寓居空间为或置身于宦场、或辗转于江湖中的他提供了化解当下困境的精神力量之源。

关键词：苏轼寓居空间心境黄州