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Carrier-Envelope Phase-Dependent Atomic Coherence and Its Detection Scheme

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Communications in Theoretical Physics 2008年第4期49卷 1022-1024页

作者： yang Xiao-xue WU ying Physics Department Huazhong University of Science and Technology Wuhan 430074 China

The carrier-envelope phase （CEP） of few-cycle laser pulses is shown to have profound effects on the bound- state atomic coherence even in the weak-field regime where ionizations hardly take place, and a simple schem... 详细信息

关键词： carrier-envelope phase ultrafast processes atomic coherence

Weak-Coupling Theory for Semiclassical Periodically Driven Two-Level Systems： Beyond Rotating-Wave Approximation

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Communications in Theoretical Physics 2007年第2X期48卷 339-342页

作者： yang Xiao-xue WU ying Department of Physics Huazhong University of Science and Technology Wuhan 430074 China

We present a weak-coupling theory of semiclassical periodically driven two-level systems. The explicit analytical approximating solution is shown to reproduce highly accurately the exact results well beyond the regime... 详细信息

关键词： periodically driven two-level systems weak-coupling theory beyond rotating-wave approximation

Cloning, Expression and Purification of Wheat Acetyl-CoA Carboxylases CT Domain in E. coil

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Chemical Research in Chinese Universities 2008年第6期24卷 752-755页

作者： WANG Rui-jian yang xue-ying ZHENG Liang-yu yang Ye GAO Gui CAO Shu-gui Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education Jilin University Changchun 130021 P. R. China College of Forestry Beihua University Jilin 132013 P. R. China

The entire gene of carboxyltransferase（CT） domain of acetyl-CoA carboxylase（ACCase） from Chinese Spring wheat（CSW） plastid was cloned firstly, and the 2.3 kb gene was inserted into PET28a^＋ vector and expressed in E. coil in a soluble state. The （His）6 fusion protein was identified by SDS-PAGE and Western blot. The recombinant protein was purified by affinity chromatography, and the calculated molecular mass（Mr） was 88000. The results of the sequence analysis indicate that the cloned gene（GeneBank accession No. EU124675） was a supplement and revision of the reported ACCase CT partial cDNA from Chinese Spring wheat plastid. The recombinant protein will be significant for us to investigate the recognizing mechanism between ACCase and herbicides, and further to screen new herbicides.

关键词： Chinese Spring wheat Acetyl-CoA carboxylase Carboxyltransferase(CT) domain Cloning Expression Purification

Design of 2.1GHz CMOS Low Noise Amplifier

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The Journal of China Universities of Posts and Telecommunications 2006年第1期13卷 71-74页

作者： LI En-ling SONG Lin-hong yang Dang-qiang xue ying CHU Meng Science School Xi'an University of Technology Xi'an 710048 P.R. China

This paper discusses the design of a fully differential 2.1 GHz CMOS low noise amplifier using the TSMC 0.25 μm CMOS process. Intended for use in 3G, the low noise amplifier is fully integrated and without off-chip components. The design uses an LC tank to replace a large inductor to achieve a smaller die area, and uses shielded pad capacitances to improve the noise performance. This paper also presents evaluation results of the design.

关键词： LNA LC tank noise figure shielded pads

A Simple Method to Obtain Exact Soliton Solutions for a Nonlinear Equation in a Loss Fibre System

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Communications in Theoretical Physics 2002年第11期 615-616页

作者： yang Xiao-xue WU ying Department of Physics and National Key Laboratory for Laser Technique Huazhong University of Science and Technology Wuhan 430074 China Department of Physics and National Key Laboratory for Laser Technique Huazhong University of Science and Technology Wuhan 430074 China Wuhan Institute of Physics and Mathematics the Chinese Academy of Sciences Wuhan 430071 China

We show that the nonlinear equation governing wave propagation in a loss fibre system considered byNakkeeran in J. Phys. A34 (2001) 5111 can be brought into the standard nonlinear Schrodinger equation by a simpletrans... 详细信息

关键词： nonlinear optics, fibre communication

Refolding and Purification of Yeast Acetyl-CoA Carboxylases CT Domain Expressed as Inclusion Bodies in Escherichia coli

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Chemical Research in Chinese Universities 2009年第5期25卷 690-694页

作者： yang xue-ying TAO Jin ZHENG Liang-yu WANG Rui-jian ZHAO Ke CAO Shu-gui Key Laboratory for Molecular Enzymology and Engineering Ministry of Education Jilin University Changchun 130021 P. R. China

Acetyl-CoA carboxylase（ACCase） is a crucial enzyme in fatty acid synthesis, by regulating the first committed step in the process. Therefore, it is a potential target for the development of new compounds against obesity or as herbicides. The cDNA encoding yeast ACCase CT domains（YCTs） from Saccharvmyces cerevisiae was amplified by RT-PCR and inserted into the vector PET28a（＋） for bacterial expression of YCT fused to N-terminal His-tag（YCT-his6）. YCTs-his6 was expressed in Escherichia coli BL21（DE3） PLys as inclusion bodies, which was solubilized in 8 mol/L urea. Ni-agarose chromatography was used to purify the inclusion bodies under denaturing condition. Correct refolding was achieved via systematic dialysis to remove the denaturant gently in the presence of 0.5 mmol/L Triton X-100. The low concentration Triton X-100 was included in the refolding buffer to increase the solubilization and enhance dimeric formation of refolding proteins. The activity of the refolded YCT-his6 was 1.2 U/rag as measured in a spectrophotometric assay using malonyl-CoA as the substrate. To our knowledge, it is the first time that the bioactive YCT-his6 has been expressed successfully in E. coli and isolated from their inclusion bodies.

关键词： Carboxyltransferase domain Expression Inclusion body Refolding

Strain Mesorhizobium ***035:A Stress-Tolerant Isolate from Glycyrrhiza glabra Displaying a Wide Host Range of Nodulation

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Pedosphere 2008年第1期18卷 102-112页

作者： WEI Ge-Hong yang xue-ying ZHANG Zhi-Xin yang Ya-Zhen K. LINDSROM Shaanxi Key Laboratory of Molecular Biology for Agriculture College of Life Science Northwest A＆F University Yangling Shaanxi 712100 （China） Department of Applied Chemistry and Microbiology Biocenter 1 Fin-0014 University of Helsinki （Finland）

Thirty-nine rhizobial isolates were isolated from the root nodules of Glycyrrhiza uralensis and Glyeyrrhiza glabra, growing in the arid and semiarid regions of northwestern China, to test their taxonomic position and stress tolerance and to select one promising putative inoeulant strain for further studies. On the basis of 113 physiological and biochemical characteristics, the isolates were clustered into three groups. One isolate CCNWGX035 was found to have high tolerance to NaC1, pH, and temperature. By sequencing the 16S rDNA, isolate CCNWGX035 was placed in genus Mesorhizobium. Nodulation tests demonstrated that the isolate not only formed nitrogen-fixing nodules on its original host plant Glyeyrrhiza glabra, but also on Sophora vieiifolia, Lotus cornieulatus, Trifolium repens, Melilotus suaveolens, and Sophora alopecuroides. On the basis of sequence analysis of the nodA gene, isolate CCNWGX035 was closely related to strains of the genus Mesorhizobium, exhibiting some novel characteristics of root nodule bacteria.

关键词： Glycyrrhiza host range Mesorhizobium nodA stress tolerance

The impact of climate change on forest fire danger rating in China’s boreal forest

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Journal of Forestry Research 2011年第2期22卷 249-257页

作者： yang Guang DI xue-ying GUO Qing-xi SHU Zhan ZENG Tao YU Hong-zhou WANG Chao College of Forestry Northeast Forestry University Harbin 150040 P.R.China.

The Great Xing＇an Mountains boreal forests were focused on in the northeastern *** simulated future climate scenarios of IPCC SRES A2a and B2a for both the baseline period of 1961-1990 and the future scenario periods were downscaled by the Delta Method and the Weather Generator to produce daily weather *** the verification with local weather and fire data,the Canadian Forest Fire Weather Index System was used to assess the forest fire weather situation under climate change in the study *** increasing trend of fire weather severity was found over the 21st century in the study region under the both future climate change scenarios,compared to the 1961-1990 baseline *** annual mean/maximum fire weather index was predicted to rise continuously during 2010-2099,and by the end of the 21st century it is predicted to rise by 22%-52% across much of China＇s boreal *** significant increases were predicted in the spring from of April to June and in the summer from July to *** the summer,the fire weather index was predicted to be higher than the current index by as much as 148% by the end of the 21st *** the scenarios of SRES A2a and B2a,both the chance of extremely high fire danger occurrence and the number of days of extremely high fire danger occurrence was predicted to increase in the study *** is anticipated that the number of extremely high fire danger days would increase from 44 days in 1980s to 53-75 days by the end of the 21st century.

关键词： climate change wildfire boreal forest fire weather species West Africa

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莓状黄铁矿:环境与生命的示踪计

地球科学（中国地质大学学报） 2011年第4期36卷 643-658页

作者：杨雪英龚一鸣中国地质大学生物地质与环境地质国家重点实验室湖北武汉430074

莓状黄铁矿这一奇妙的微晶（0．1～1μm）矿物集合体（5-50μm）自科学家首次发现（1923年）和冠名（1935年）至今一直是不同学科竞相研究的热点．主要从莓状黄铁矿的形成机制、莓状黄铁矿与环境的关系等方面回顾了莓状黄铁矿的生物可... 详细信息

莓状黄铁矿这一奇妙的微晶（0．1～1μm）矿物集合体（5-50μm）自科学家首次发现（1923年）和冠名（1935年）至今一直是不同学科竞相研究的热点．主要从莓状黄铁矿的形成机制、莓状黄铁矿与环境的关系等方面回顾了莓状黄铁矿的生物可因说（1923—1969年）、非生物成因说（1969-2000年）和多元成因说（2000-现今）各研究阶段取得的进展和存在的问题，指出莓状黄铁矿作为表层生物圈、深部生物圈和地外环境与生命示踪计具有巨大潜力，提出从地球科学、生命科学、材料科学、刊学和纳米科技以及凝聚态物理学融合的角度加强对莓状黄铁矿研究的建议．

关键词：莓状黄铁矿生物成因非生物成因硫同位素氧化还原环境