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Biomedical and Environmental Sciences 2020年第8期33卷 620-622页

作者： XIAO Kang yang xue hua ZOU Wen Quan DONG Xiao Ping SHI Qi State Key Laboratory for Infectious Disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases(Zhejiang University)National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Center for Global Public Health Chinese Center for Disease Control and PreventionBeijing 102206China Wuhan Institute of Virology Chinese Academy of ScienceWuhan 430071Hubei China

Real-time quaking-induced conversion(RT-QuIC)assay is a newly established PrPSc-detecting *** development of RT-QuIC improves the diagnosis of sporadic Creutzfeldt–Jakob disease(sCJD),showing good sensitivity and specificity in many countries when the method was used in cerebrospinal fluid(CSF)***,in China,the sensitivity and specificity of RT-QuIC has yet to be determined due to the lack of definitive diagnosis samples.

关键词： diagnosis CJD cerebrospinal

Preparation of PrP-specific Polyclonal Antibody via Immunization of PRNP-knockout Mice with Recombinant Human PrP Protein

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Biomedical and Environmental Sciences 2020年第7期33卷 493-501页

作者： yang xue hua WU Yue Zhang XIAO Kang GAO Li Ping CHEN Dong Dong DONG Xiao Ping SHI Qi State Key Laboratory for Infectious Disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases(Zhejiang University)National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Center for Global Public Health Chinese Center for Disease Control and PreventionBeijing 102206China Wuhan Institute of Virology Chinese Academy of ScienceWuhan 430071HubeiChina China Academy of Chinese Medical Sciences Beijing 100700China

Objective The definite diagnosis of human and animal prion diseases depends on the examination of special pathological changes and/or detection of PrPSc in the brain tissues of suspected ***,developing methods to obtain PrP antibody with good specificity and sensitivity is fundamental for prion *** We prepared a PrP-specific polyclonal antibody(pAb P54)in a PRNP-knockout mouse model via immunization with recombinant full-length human PrP protein residues 23–***,we verified that pAb in Western blot,immunohistochemistry(IHC),and immunofluorescent(IFA)*** Western blot illustrated that the newly prepared pAb P54 could react with recombinant PrP protein,normal brain PrPC from healthy rodents and humans,and pathological PrPSc in the brains of experimental rodents infected with scrapie and humans infected with different types of prion *** electrophoretic patterns of brain PrPC and PrPSc observed after their reaction with pAb P54 were nearly identical to those produced by commercial PrP monoclonal *** glycosylated PrP molecules in the brain homogenates were clearly demonstrated in the reactions of these molecules with pAb *** assay revealed apparent PrP deposits in the GdnCl-treated brain slices of 139 A-infected mice and 263 K-infected *** tests with pAb P54 also showed clear green signals surrounding blue-stained cell *** The newly prepared pAb P54 demonstrated reliable specificity and sensitivity and,thus,may have potential applications not only in studies of prion biology but also in the diagnosis of human and experimental rodent prion diseases.

关键词： Prion disease PrP Antibody PRNP-knockout mouse

Global Profiles of Acetylated Proteins in Brains of Scrapie Agents 139A- and ME7-Infected Mice Collected at Mid-Early, Mid-Late, and Terminal Stages

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Biomedical and Environmental Sciences 2022年第8期35卷 722-734页

作者： SHI Qi CHEN Dong Dong ADALATI Maimaitiming XIAO Kang GAO Li Ping yang xue hua WU Yue Zhang CHEN Cao DONG Xiao Ping State Key Laboratory of Infectious Disease Prevention and Control NHC Key Laboratory of Medical Virology and Viral DiseasesNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBejing 102200China Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Zhejiang UniversityHangzhou 310014ZhejiangChina China Academy of Chinese Medical Sciences Bejing 10000China Center for Biosafety Mega-Science Chinese Academy of SciencesWuhan 43000HubeiChina Shanghai Institute of lnfectious Disease and Biosafety Shanghai 201318China

Objective To describe the global profiles of acetylated proteins in the brains of scrapie agents 139Aand ME7-infected mice collected at mid-early,mid-late,and terminal *** The acetylated proteins from the cortex regions of scrapie agent(139A-and ME7)-infected mice collected at mid-early(80 days postinfection,dpi),mid-late(120 dpi),and terminal(180 dpi) stages were extracted,and the global profiles of brain acetylated proteins were assayed with proteomic mass *** proteins in the infected mice showing 1.5-fold higher or lower levels than that of agematched normal controls were considered as differentially expressed acetylated peptides(DEAPs).Results A total of 118,42,and 51 DEAPs were found in the brains of 139A-80,139A-120,and 139A-180dpi mice,***,390,227,and 75 DEAPs were detected in the brains of ME7-80,ME7-120,and ME7-180 dpi mice,*** overwhelming majority of DEAPs in the mid-early stage were down-regulated,and more portions of DEAPs in the mid-late and late stages were *** 22.1%(328/1,485) of acetylated peptides mapped to 74 different proteins were mitochondrial *** Encyclopedia of Genes and Genomes pathway analysis identified 39(80dpi),13(120 dpi),and 10(180 dpi) significantly changed pathways in 139A-infected ***,55,25,and 18 significantly changed pathways were observed in the 80,120,and 180 dpi samples of139A-and ME7-infected mice(P < 0.05),*** pathways were commonly involved in all tested ***,many steps in the citrate cycle(tricarboxylic acid cycle) were affected,represented by down-regulated acetylation for relevant enzymes in the mid-early stage and upregulated acetylation in the mid-late and late *** Our data here illustrated the changes in the global profiles for brain acetylated proteins during prion infection,showing remarkably inhibited acetylation in the early stage and relatively enhanced acetylation in the la

关键词： Prion Scrapie-infected mouse Acetylation Proteomics Kyoto Encyclopedia of Genes and Genomes pathway

The Red Edge Parameters of Different Wheat Varieties Under Different Fertilization and Irrigation Treatments

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Agricultural Sciences in China 2002年第7期1卷 745-751页

作者： ZHAO Chun-Jiang, huaNG Wen-Jiang, WANG Ji-hua, yang Min-hua and xue Xu-zhang( National Engineering Center for Information Technology in Agriculture , Beijing 100089 , P. R . China) National Engineering Center for Information Technology in Agriculture Beijing 100089 P. R. China

The dynamics of red edge parameters at different growth stages of wheat canopy was studied. The red edge position moved to the longer wavelength from erecting stage to heading stage, and returned to the original wavelength direction from grain-filling stage to maturity stage. The leaf total nitrogen content (LTN) is positively related to red edge amplitude (d λ red) and NIR platform amplitude (d λNIRP) and the correlation coefficient improved as growth stage extended. The chlorophyll content (TChl) is negatively related to d λ red from erecting stage to the elongation stage, and positively related to d λ red from heading stage to milky maturity stage. TChl is also positively related to d λNIRP. The leaf area index (LAI) is positively related to d λ red and d λNIRP, and the correlation coefficient improved as growth stage extended. It was concluded that d λ red and d λNIRP can be used as the indicators of winter wheat growth state, and guide the fertilization and irrigation decision making. The λNIRF is suitable for derivation of LTN, the d λ red for TChl, and the d λ red or d λNIRP for LAI.

关键词： Winter wheat Spectrum Red edge parameters (REP) Variety Fertilizer Water

氯诺昔康F127原位凝胶剂体内外释药相关性的研究

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药物生物技术 2018年第3期25卷 201-205页

作者：杨雪华杨莹黄桂华山东大学药学院山东济南250012

将普朗尼克F127和F68联合应用作为凝胶基质制备载氯诺昔康的原位凝胶剂,并对其体内外释药相关性进行研究。以胶凝温度、胶凝时间和通针性为评价指标,采用冷溶法制备氯诺昔康原位凝胶剂。首先,通过单因素考察筛选空白原位凝胶和载药原位... 详细信息

将普朗尼克F127和F68联合应用作为凝胶基质制备载氯诺昔康的原位凝胶剂,并对其体内外释药相关性进行研究。以胶凝温度、胶凝时间和通针性为评价指标,采用冷溶法制备氯诺昔康原位凝胶剂。首先,通过单因素考察筛选空白原位凝胶和载药原位凝胶的最佳处方,并对其理化性质进行表征;然后,采用动态膜透析法测定氯诺昔康原位凝胶剂的体外释药性,以大鼠为研究对象,皮下注射给药研究氯诺昔康原位凝胶剂的体内释药性;最后,采用体外释放曲线与体内吸收曲线各时间点分别相关的方法评价其体内外相关性。结果表明最佳处方的原位凝胶剂含20%的F127、2.3%的F68,且为假塑性流体,胶凝温度为36℃,胶凝时间为(252±1.53)s,通针性好;12 h体外累积释放百分率为82.33%,与氯诺昔康溶液相比具有明显缓释效果;大鼠皮下注射给药后,由体内血药浓度-时间曲线可知,与溶液相比,氯诺昔康原位凝胶剂在皮下滞留量较多;采用Loo-Riegelman法计算体内吸收百分数,评价原位凝胶剂的体外释放与体内吸收的相关性,相关系数为r=0.964 1。说明氯诺昔康F127原位凝胶剂制备工艺简单,大鼠皮下注射给药体内外相关性良好。

关键词：氯诺昔康温敏型原位凝胶普朗尼克F127 普朗尼克F68 局部注射体内外相关性

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提升机钢绳多层缠绕平稳过渡的研究

煤炭学报 2000年第2期25卷 212-216页

作者：龚宪生杨雪华夏荣海重庆大学机械传动国家重点实验室重庆400044 南桐矿务局重庆400802 中国矿业大学北京校区北京100083

从钢绳多层缠绕过渡过程入手 ,运用微分几何、力学、数学分析的方法 ,研究钢绳过渡情况 ,提出层间过渡块的几何形状和计算公式及钢绳圈间过渡长度和角度理论公式 .实验证明 ,按本文提出的公式设计过渡块和过渡区 ,能使过渡钢绳的冲击振... 详细信息

从钢绳多层缠绕过渡过程入手 ,运用微分几何、力学、数学分析的方法 ,研究钢绳过渡情况 ,提出层间过渡块的几何形状和计算公式及钢绳圈间过渡长度和角度理论公式 .实验证明 ,按本文提出的公式设计过渡块和过渡区 ,能使过渡钢绳的冲击振动和磨损大大降低 ,并保证圈间过渡平稳进行 .

关键词：提升机钢绳多层缠绕过渡振动

Prospects of elafibranor in treating alcohol-associated liver diseases

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World Journal of Gastroenterology 2025年第2期31卷 132-137页

作者： Wei-Tong Cui hua-Ru xue Dian-Fang Wei Xiao-Yu Feng Kai Wang School of Basic Medicine Qilu Medical UniversityZibo 255300Shandong ProvinceChina School of Medical Imaging Qilu Medical UniversityZibo 255300Shandong ProvinceChina

Alcohol-related liver disease(ALD),which is induced by excessive alcohol con-sumption,is a leading cause of liver-related morbidity and *** pa-tients exhibit a spectrum of liver injuries,including hepatic steatosis,inflam-mation,and fibrosis,similar to symptoms of nonalcohol-associated liver diseases such as primary biliary cholangitis,metabolic dysfunction-associated steatotic liver disease,and nonalcoholic *** has been approved for the treatment of primary biliary cholangitis and has been shown to improve symptoms in both animal models and in vitro cell models of metabolic dysfunc-tion-associated steatotic liver disease and nonalcoholic ***,the efficacy of elafibranor in treating ALD remains *** this article,we comment on the recent publication by Koizumi et al that evaluated the effects of elafibranor on liver fibrosis and gut barrier function in an ALD mouse *** findings indicate the potential of elafibranor for ALD treatment,but further experimental investigations and clinical trials are warranted.

关键词： Elafibranor Alcohol-associated liver diseases Peroxisome proliferator-activated receptor Lipid Apoptosis Steatosis Inflammation Fibrosis

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汾河上游水文干旱与气象干旱的关系

水电能源科学 2018年第9期36卷 19-22页

作者：皮卫阳赵雪花祝雪萍太原理工大学水利科学与工程学院山西太原030024

为分析汾河上游水文干旱与气象干旱的关系,基于汾河上游4个气象站1956~2000年月降水量、月平均气温资料建立流域标准化降水指数(SPI)和标准化降水蒸散指数(SPEI),并根据4个水文站同期月径流资料建立各水文站标准化径流指数(SRI);对1、3... 详细信息

为分析汾河上游水文干旱与气象干旱的关系,基于汾河上游4个气象站1956~2000年月降水量、月平均气温资料建立流域标准化降水指数(SPI)和标准化降水蒸散指数(SPEI),并根据4个水文站同期月径流资料建立各水文站标准化径流指数(SRI);对1、3、6、12个月4个时间尺度的指数进行趋势分析,然后分析各水文站4个时间尺度的SRI与流域SPI、SPEI的相关性,最后采用时移互相关分析、回归分析研究水文干旱对气象干旱的滞后性。结果表明,汾河上游呈干旱化趋势;各站水文干旱与气象干旱间的相关系数随时间尺度的增加而增加,都通过了显著性检验;相同时间尺度下,4个水文站水文干旱与气象干旱相关程度差异较弱;SRI与SPEI的相关程度较SRI与SPI更为紧密;12个月时间尺度下,汾河水库、寨上、兰村SRI表征的水文干旱滞后于以SPEI表征的气象干旱约1个月。

关键词：干旱标准化降水指数标准化降水蒸散指数标准化径流指数相关分析回归分析

胃癌的X线、螺旋CT三期增强扫描与病理学对照研究

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中国医学影像技术 2001年第4期17卷 295-297页

作者：高剑波 yang xue-hua GUO hua 杨学华郭华李荫太陈学军管生杨晓鹏河南医科大学第一附属医院放射科河南郑州450052 Department of Radiology the First Teaching Hospital of Henan Medical University

目的　探讨胃癌的螺旋CT强化特征 ,评价口服水螺旋CT三期增强扫描对胃癌分期及大体分型的诊断价值。方法　对 44例进展期胃癌患者实施胃气钡双对比造影及口服水螺旋CT三期增强扫描 ,并与术后病理对照。结果　① 44例胃癌中 ,32例以门静... 详细信息

目的　探讨胃癌的螺旋CT强化特征 ,评价口服水螺旋CT三期增强扫描对胃癌分期及大体分型的诊断价值。方法　对 44例进展期胃癌患者实施胃气钡双对比造影及口服水螺旋CT三期增强扫描 ,并与术后病理对照。结果　① 44例胃癌中 ,32例以门静脉期强化程度为著 ,与动脉期比较有显著性差异 (t =4.2 2 6 ,P <0 .0 5 )。②钡餐造影及螺旋CT对胃癌分型总的准确率分别为 75 .0 %和 86 .5 %。③螺旋CT三期增强扫描对胃癌TNM分期的准确性为 81.8%。结论　螺旋CT对胃癌大体分型的准确性优于钡餐造影 ,对胃癌术前TNM分期的准确性明显优于普通CT。

关键词：螺旋CT X线病理学胃癌

N-乙酰半乳糖胺及其衍生物作为小干扰核糖核酸和反义寡核苷酸递送载体的研究进展

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中国新药杂志 2023年第13期32卷 1336-1344页

作者：李大伟毛楷凡杨雪华薛松刘正平山东省药学科学院济南250101 国家药品监督管理局药物制剂技术研究与评价重点实验室济南250101 山东省皮肤与黏膜给药工程技术研究中心济南250101

近年来,使用N-乙酰半乳糖胺(GalNAc)缀合物靶向去唾液酸糖蛋白受体来递送寡核苷酸至肝细胞已成为治疗性寡核苷酸领域的突破方法。与传统的寡核苷酸递送方法相比,GalNAc偶联技术是一种更简单的肝脏递送方法,表现出肝靶向特异性、高效性... 详细信息

近年来,使用N-乙酰半乳糖胺(GalNAc)缀合物靶向去唾液酸糖蛋白受体来递送寡核苷酸至肝细胞已成为治疗性寡核苷酸领域的突破方法。与传统的寡核苷酸递送方法相比,GalNAc偶联技术是一种更简单的肝脏递送方法,表现出肝靶向特异性、高效性、安全性以及可规模化制备等优势,具有深入开发的研究价值和临床应用的广阔前景。利用GalNAc偶联技术开发的givosiran已被美国FDA批准用于治疗急性肝卟啉症,此外还有7种缀合物处于注册审评或Ⅲ期临床试验中,另外至少还有21种GalNAc核酸缀合物处于临床开发的早期阶段。但国内GalNAc偶联技术相关研究基础比较薄弱,也无相关产品上市,属于“卡脖子”技术,因此本综述重点介绍了GalNAc及其衍生物作为小干扰核糖核酸(siRNA)和反义寡核苷酸(ASO)这2种寡核苷酸递送载体的研究进展,以期为国内GalNAc缀合物的开发提供参考。

关键词： N-乙酰半乳糖胺小干扰核糖核酸反义寡核苷酸临床前研究临床研究上市情况