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Chinese Journal of Natural Medicines 2015年第10期13卷 791-795页

作者： ZHU Hui XU Liang yang shi-lin LI He-Ran College of Pharmaceutical Sciences Soochow University

The stereochemistry of two 6, 9-oxygen bridge dibenzocyclooctadiene lignans from Kadsura coccinea, are difficult to separate and very unstable. The present study was designed to develop a high-performance liquid chromatography using circular dichroism detection for the analysis of the stereochemistry. A new 6, 9-oxygen bridge dibenzocyclooctadiene lignans named Kadsulignan Q was firstly found with an S-biphenyl configuration. The other compound was identified as Kadsulignan L with an R- biphenyl configuration. In order to obtain kinetic data on their reversible interconversion, the stability was measured at different deuterated solvents such as deuterated methanol, deuterated chloroform and deuterated dimethylsulfoxide. The lignans were more unstable and converted more easily in deuterated methanol than in deuterated chloroform and deuterated dimethylsulfoxide.

关键词： 6,9-Oxygen bridge dibenzocyclooctadiene lignans Kadsura coccinea High-performance liquid chromatography using circular dichroism detection(HPLC-CD) Kadsulignan Q Kadsulignan L

Antioxidant and cardioprotective effects of Ilex cornuta on myocardial ischemia injury

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Chinese Journal of Natural Medicines 2017年第2期15卷 94-104页

作者： YUAN Yan PAN Shu yang shi-lin LIU Yan-Li XU Qiong-Ming Department of Pharmacology College of Pharmaceutical ScienceSoochow UniversitySuzhou 215123China Department of Pharmaceutical Chemistry Guizhou UniversityGuiyang 550025China Department of Pharmacognosy College of Pharmaceutical ScienceSoochow UniversitySuzhou 215123China

Previous studies have indicated that the Ilex genus exhibits antioxidant, neuroprotective, hepatoprotective, and antiinflammatory activities. However, the pharmacologic action and mechanisms of Ilex cornuta against cardiac diseases have not yet been explored. The present study was designed to investigate the antioxidant and cardioprotective effects of Ilex cornuta root with in vitro and in vivo models. The anti-oxidative effects of the extract of Ilex cornuta root(ICR) were measured by 2, 2-diphenyl-1- picrylhydrazyl(DPPH) free-radical scavenging and MTT assays as well as immunoassay. Furthermore, a rat model of myocardial ischemia was established to investigate the cardioprotective effect of ICR in vivo. Eight compounds were isolated and identified from ICR and exhibited DPPH free-radical scavenging activities. They also could increase cell viability and inhibit morphological changes induced by H_2O_2 or Na_2S_2O_4 in H9c2 cardiomyocytes, followed by increasing the SOD activities and decreasing the MDA and ROS levels. In addition, it could suppress the apoptosis of cardiomyocytes. In the rat model of myocardial ischemia, ICR decreased myocardial infarct size and suppressed the activities of LDH and CK. Furthermore, ICR attenuated histopathological alterations of heart tissues and the MDA levels, while increasing SOD activities in serum. In conclusion, these results suggest that ICR has cardioprotective activity and could be developed as a new food supplement for the prevention of ischemic heart disease.

关键词： Ilex cornuta Myocardial ischemia Antioxidant Apoptosis

Three new triterpenoids isolated from the aerial parts of Ilex cornuta and protective effects against H2O2-induced myocardial cell injury

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Chinese Journal of Natural Medicines 2017年第2期15卷 115-120页

作者： LI Shan-Shan LIU Yan-Li XU Qiong-Ming MAO Chen-Mei yang shi-lin Children's Hospital of Soochow University Suzhou 215003China College of Pharmaceutical Sciences Soochow UniversitySuzhou 215123China

In the present study, three new triterpenoids, 23-hydroxyurs-12, 18-dien-28-oic acid 3β-O-α-L-arabinopyranoside(1), 23-hydroxyurs-12, 18-dien-28-oic acid 3β-O-β-D-glucuronopyranoside-6-O-methyl ester(2), and urs-12, 18-dien-28-oic acid 3β-O-β-D-glucuronopyranoside-6-O-methyl ester(3), and a known triterpenoid, 3β-hydroxy-urs-2, 18-dien-28-oic acid(4, randialic acid B), were isolated from the aerial parts of Ilex cornuta. Their structures were identified by the spectroscopic analyses(IR, ESI-MS, HR-ESI-MS, and 1D and 2D NMR) and chemical reactions. Compound 4 showed significant cell-protective effects against H_2O_2-induced H9c2 cardiomyocyte injury. Compounds 1-4 did not show any significant DPPH radical scavenging activity.

关键词： Ilex cornuta Aquifoliaceae Triterpenoids Cardiomyocytes Oxidative injury

Isolation and Characterization of Two New Compounds from Lysimachia Davurica

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Chemical Research in Chinese Universities 2005年第5期21卷 549-551页

作者： TIAN Jing-kui ZOU Zhong-mei XU Li-zhen yang shi-lin Department of Chinese Medicine Science and Engineering Zhefiang University Hangzhou 310027P. R. China Institute of Medicinal Plants Development Chinese Academy of Medical Scienees and Peking Union Medical College Beijing 100094 P. R. China

Two new saponins named davuricoside H and davuricoside K were isolated from the whole plant of Lysimachia davurica. The structures of davuricoside H and davuricoside K were determined by 1-D and 2-D NMR, MS techniques, and chemical methods to be 3β,16α ,28-trihydroxy-olean-12-en-30-oic acid-3-O-β-D-glucopyranosyl-（ 1→2 ）- [β-D-glucopyranosyl-（ 1 → 4 ） ] -α-L-arabinopyranoside and 3β,16or ,28-trihydroxy-olean-12-en-30-oic acid-3-O-{β-D- glucopyranosyl- （ 1 →2 ） - [β-D-glucopyranosyl- （ 1 →4 ） ] -α-L-arabinopyranosyl } -30-O-β-D-glucopyranosyl-ester.

关键词： Lysimachia davurica Triterpenoid saponin Davuficoside H Davuricoside K

Identification and characterization of two new xanthones from Cudrania fruticosa

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Chinese Journal of Natural Medicines 2015年第10期13卷 781-785页

作者： LIANG Bo XU Li-Zhen yang shi-lin GONG Xiao-Jie Medical College Dalian University Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical College

The present study was designed to investigate the chemical constituents of the roots of Cudrania fruticosa Wight. Compounds were isolated by various column chromatographic methods including silica gel, polyamide, sephadex LH-20, and semi-preparative HPLC. Their structures were elucidated by a combination of 1D and 2D NMR techniques, mass spectrometry, and chemical methods. Two new xanthones, Cudraxanthone T and U(1-2), along with four known compounds(3-6) were isolated from the roots of Cudrania fruticosa Wight.

关键词： Cudrania fruticosa Xanthones Cudraxanthone T Cudraxanthone U

New triterpenoid sapoin from Ardisia gigantifolia Stapf.

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Chinese Chemical Letters 2010年第4期21卷 449-452页

作者： Gong, Qiang Qiang Mu, Li Hua Liu, Ping yang, shi lin Wang, Bo Feng, Yu lin Gen Hosp PLA Dept Clin Pharmacol Beijing 100853 Peoples R China JiangXi Univ Tradit Chinese Med Nanchang 330006 Peoples R China

One new triterpenoid sapoin with two known triterpenoid sapoins:3β-O-{α-L-rhamnopyranosyl-(1→3)-[β-D-xylopyranose-(1→2)]-β-D-glucopyranosyl-(1→4)-α-L-arabinopyranosyl}-3β-hydroxy-13β,28-epoxy-oleanan-16-oxo-30-al(1),3β-O-{α-L-rhamnopyranosyl-(1→3)-[(β-D-xylopyranosyl-(1→2)]-β-D-galactopyranosyl-(1→4)-[(β-D-glucopyranosyl-(1→2)]-α-L-ara-binopyranoside}-16α-hydroxy-13,28-epoxy-oleanane(2) and cyclamiretin A 3β-O-α-L-rhamnopyranosyl-(l→3)-[P-D-xylopyranosyl-(1→2)]-β-D-glucopyranosyl-(1→4)-[β-D-glucopyranosyl-(...

关键词： Ardisia gigantifolia Stapf. Myrsinaceae Triterpenoid sapoin

Laboratory Evaluation of the Molluscicidal Activity of Pulsatilla Chinensis(Bunge)Regel Saponins against the Snail Oncomelania Hupensis

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Biomedical and Environmental Sciences 2012年第2期25卷 224-229页

作者： CHEN Yan Qin XU Qiong Ming LIU Yan Li LI Xiao Ran yang shi lin ZHUGE Hong Xiang Department of Parasitology Medical College of Soochow UniversitySuzhou 215123JiangsuChina Medical College of Nanyang Nanyang 473000HenanChina College of Pharmacy Medical College of Sooehow UniversitySuzhou 215123JiangsuChina

Objective To observe the toxicity of Pulsatilla chinensis（Bunge） Regel saponins（PRS） against Oncomelania hupensis（O.hupensis）.Methods O.hupensis snails were exposed to 40% and 80% of 24 h LC50 of PRS for 24 h,and then choline esterase（CHE）,alanine aminotransferase（ALT）,alkaline phosphatase（ALP）,lactate dehydrogenase（LDH） activities in cephalopodium and liver of snails were determined.Niclosamide（NIC） was used as the reference molluscicide.Zebra fish lethality test was evaluated to non-target aquatic species of PRS.Results The molluscicidal activity of PRS（LC50 at 24 h：0.48 mg/L） was similar to that of NIC（LC50 at 24 h：0.16 mg/L）.Significant alterations about CHE,ALP,and ALT activities both in the cephalopodium and the liver of snails were observed when O.hupensis was exposed to 40% and 80% LC50 of PRS or NIC for 24 h.PRS and NIC could not affect LDH activity in the cephalopodium and the liver.Lower toxicity to fish of PRS was observed up to the highest concentration tested than NIC.Conclusion PRS,as compared with the reference molluscicide NIC,is thought to be used for the control of harmful vector snails safely.

关键词： Oncomelania hupensis Molluscicidal activity Saponin Pulsatilla chinensis (Bunge) Regel Niclosamide

Effects of ultrahigh pressure extraction on yield and antioxidant activity of chlorogenic acid and cynaroside extracted from flower buds of Lonicera japonica

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Chinese Journal of Natural Medicines 2015年第6期13卷 445-453页

作者： HU Wen GUO Ting JIANG Wen-Jun DONG Guang-Li CHEN Da-Wei yang shi-lin LI He-Ran College of Pharmacy Soochow University Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application Department of Polymer Science and Engineering College of Chemistry Chemical Engineering and Materials Science Soochow University Guiding Long Da Honeysuckle Technology Co. Ltd.

The present study was designed to establish and optimize a new method for extracting chlorogenic acid and cynaroside from Lonicera japonica Thunb. through orthogonal experimental designl. A new ultrahigh pressure extraction(UPE) technology was applied to extract chlorogenic acid and cynaroside from L. japonica. The influential factors, including solvent type, ethanol concentration, extraction pressure, time, and temperature, and the solid/liquid ratio, have been studied to optimize the extraction process. The optimal conditions for the UPE were developed by quantitative analysis of the extraction products by HPLC-DAD in comparison with standard samples. In addition, the microstructures of the medicinal materials before and after extraction were studied by scanning electron microscopy(SEM). Furthermore, the extraction efficiency of different extraction methods and the 2, 2-diphenyl-1-picrylhydrazyl(DPPH) radical scavenging activities of the extracts were investigated. The optimal conditions for extracting chlorogenic acid and cynaroside were as follows: ethanol concentration, 60%; extraction pressure, 400 MPa; extraction time, 2 min; extraction temperature, 30 °C; and the solid/liquid ratio, 1 : 50. Under these conditions, the yields of chlorogenic acid and cynaroside were raised to 4.863% and 0.080%, respectively. Compared with other extraction methods, such as heat reflux extraction(HRE), ultrasonic extraction(UE), and Sohxlet extraction(SE), the UPE method showed several advantages, including higher extraction yield, shorter extraction time, lower energy consumption, and higher purity of the extracts. This study could help better utilize L. japonica flower buds as a readily accessible source of natural antioxidants in food and pharmaceutical industries.

关键词：忍冬装饰用的梨树 Thunb 超离频压力抽取 Chlorogenic 酸 Cynaroside 抗氧化剂活动

The ethyl acetate extraction of Pileostegia tomentella(ZLTE) exerts anti-cancer effects on H1299 cells via ROS-induced canonical apoptosis

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Chinese Journal of Natural Medicines 2020年第7期18卷 508-516页

作者： FAN Qiu-Mei ZHAO Wen-Tong YUAN Renyikun WANG Qin-Qin ZHANG Li-Feng GAO Hong-Wei LENG Jing yang shi-lin College of Pharmacy Guangxi University of Chinese MedicineNanning 530000China Guangxi Engineering Technology Research Center of Advantage Chinese Patent Drug and Ethnic Drug Development Nanning 530200China State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment Jiangxi University of Traditional Chinese MedicineNanchang 330004China Guangxi Key Laboratory of Translational Medicine fo r Treating High-Incidence Infectious Diseases with Integrative Medicine NanningGuangxi 530200China Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica Nanning 530200China

Lung cancer is the leading cause of cancer death and the most common malignant tumor, the long-term survival of which has stagnated in the past several decades.Pileostegia tomentella Hand.Mazz is a traditional Chinese medicine called "Zhongliuteng"(ZLT) in the pharmacopeia, which has been proved to possess a potent anti-tumor effect on various cancers.In this study, the effects of ZLT N-butanol extraction(ZLTN) and ZLT ethyl acetate extraction(ZLTE) on the viability of non-small cell lung cancer cell(NSCLC) lines H1299 and A549 were evaluated.Here, we firstly reported that ZLTE significantly inhibited H1299 cells growth without affecting the release of lactate dehydrogenase(LDH).In addition, ZLTE induced caspase-dependent apoptosis in a concentration-dependent manner and increased the expression cleaved-PARP and decreased pro-caspase-3, pro-caspase-7, pro-caspase-8, and pro-caspase-9.Moreover, ZLTE increased the level of cellular reactive oxygen species(ROS) in H1299 cells to lead to apoptosis,which was reversed by N-acetyl-cysteine(NAC).Taken together, our results revealed that ZLTE induced caspase-dependent apoptosis via ROS generation, suggesting that ZLTE is a promising herbal medicine for the treatment of NSCLC.

关键词： Pileostegia tomentella Lung cancer Apoptosis ROS

基于临床代谢组学与蛋白质组学研究粉葛治疗轻度血脂异常的作用机制

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中国中药杂志 2024年第10期49卷 2818-2827页

作者：易建云朱卫丰管咏梅周旭陈晓凡杨世林冯育林欧阳辉江西中医药大学中药固体制剂制造技术国家工程研究中心江西南昌330006 江西中医药大学现代中药制剂教育部重点实验室江西南昌330004 江西中医药大学循证医学研究中心江西南昌330004

研究粉葛临床上治疗轻度血脂异常潜在的代谢途径和作用靶点。采用UPLC-Q-TOF-MS和EASY-nLC-timsTOF-Pro2技术对轻度血脂异常患者在基线和服药12周后的血浆样品展开代谢组学和蛋白质组学研究。通过多元统计分析比较组间差异,并对轻度血... 详细信息

研究粉葛临床上治疗轻度血脂异常潜在的代谢途径和作用靶点。采用UPLC-Q-TOF-MS和EASY-nLC-timsTOF-Pro2技术对轻度血脂异常患者在基线和服药12周后的血浆样品展开代谢组学和蛋白质组学研究。通过多元统计分析比较组间差异,并对轻度血脂异常密切相关的代谢物和蛋白与临床血脂指标分别进行相关性分析,结合基因本体(Gene Ontology,GO)功能分析和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析结果,绘制粉葛治疗轻度血脂异常可能的作用途径和靶点。结果显示,粉葛治疗后患者血浆中共有56个差异代谢物和78个差异蛋白产生回调效应,包括脂质和糖代谢相关的蛋白或代谢物(ApoB-100、9,10-DHOME、GAPDH、PGK1、PGAM1、ENO1等)、炎症与氧化应激相关的蛋白或代谢物(氧化磷脂、PLA2G7、LTA4H等)。结果表明,粉葛治疗轻度血脂异常的作用机制可能与下调ApoB-100的过表达、激活PPARα/γ、促进脂肪和甘油的分解、缓解LTB4与氧化磷脂介导的氧化应激密切相关。

关键词：粉葛轻度血脂异常代谢组学蛋白质组学脂质代谢氧化应激