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Sugarcane mosaic virus infection of model plants Brachypodium distachyon and Nicotiana benthamiana

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Journal of Integrative Agriculture 2019年第10期18卷 2294-2301页

作者： XU Jing-sheng DENG Yu-qing CHENG Guang-yuan ZHAI Yu-shan PENG Lei DONG Meng XU Qian yang yong-qing Key Laboratory of Sugarcane Biology and Genetic Breeding (Fujian) of Ministry of Agriculture and Rural Affairs Fujian Agriculture and Forestry UniversityFuzhou 350002P.R.China Key Laboratory of Ministry of Education for Genetics Breeding and Multiple Utilization of CropsCollege of Crop ScienceFujian Agriculture and Forestry UniversityFuzhou 350002P.R.China Root Biology Center Fujian Agriculture and Forestry UniversityFuzhou 350002P.R.China

Sugarcane mosaic virus (SCMV;genus Potyvirus, family Potyviridae) is a causal pathogen of sugarcane mosaic disease, and it is widespread in regions where sugarcane (Saccharum spp. hybrids) is grown. It is difficult to investigate the molecular mechanism of pathogen infection in sugarcane because of limited genomic information. Here, we demonstrated that SCMV strain FZ1 can systemically infect Brachypodium distachyon inbred line Bd21 and Nicotiana benthamiana through inoculation, double antibody sandwich enzyme-linked immunosorbent, transmission electron microscopy, and reverse transcription PCR assays. The leaves of Bd21 developed mosaic symptoms, while the leaves of N. benthamiana showed no obvious symptoms under the challenge of SCMV-FZ1. We concluded that B. distachyon inbred line Bd21 is a promising experimental model plant compared with N. benthamiana for study on the infectivity of SCMV. This is the first report on the SCMV infection of model plants B. distachyon inbred line Bd21 and N. benthamiana, which will shed light on the mechanism of SCMV infection of sugarcane and benefit sugarcane breeding against sugarcane mosaic disease.

关键词： SCMV infection sugarcane Brachypodium distachyon Nicotiana benthamiana

Marker-assisted pyramiding of soybean resistance genes R_(SC4),R_(SC8),and R_(SC14Q) to soybean mosaic virus

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Journal of Integrative Agriculture 2017年第11期16卷 2413-2420页

作者： WANG Da-gang ZHAO Lin LI Kai MA Ying WANG Li-qun yang yong-qing yang Yun-hua ZHI Hai-jian College of Agriculture Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement Crop Institute Anhui Academy of Agricultural Sciences/Anhui Key Laboratory of Crops Quality Improving

Soybean mosaic virus （SMV） is one of the major viral pathogens affecting soybean crops worldwide. Three SMV resistance genes, Rsc4, Rsc8, and Rsc14Q, have been identified and mapped on soybean chromosomes 14, 2, and 13 from Dabaima, Kefeng 1, and Qihuang 1 cultivars, respectively. Soybean cultivar Nannong 1138-2 is widely grown in the yangtze River Valley of China. In this study, crosses were made between Qihuang l^Kefeng 1 and DabaimaxNannong 1138-2. Ten simple sequence repeat （SSR） markers linked to three resistance loci （Rsc4, Rsc8, and Rsc^4Q） were used to assist pyramided breeding. Pyramided families containing three resistance loci （Rsc4, Rsc8, and Rsc14Q） were evaluated by inoculating them with 21 SMV strains from China. Results indicated that the 10 markers can be used effectively to assist the selection of resistant individuals containing Rsc4, Rsc8, and Rsc14Q. A total of 53 F6 plants were confirmed to contain three homozygous alleles conferring resistance to SMV. Five F7 homozygous pyramided families exhibited resistance to 21 strains of SMV and showed desirable agronomic traits using dual selection. The strategy of pyramiding resistance gene derived from different varieties has practical breeding value in providing broad-spectrum resistance against the existing strains of SMV in China.

关键词： soybean soybean mosaic virus resistance genes pyramiding marker-assisted breeding

Fine Mapping and Candidate Gene Analysis of Resistance Gene R_(SC3Q) to Soybean mosaic virus in Qihuang 1

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Journal of Integrative Agriculture 2014年第12期13卷 2608-2615页

作者： ZHENG Gui-jie yang yong-qing MA Ying yang Xiao-feng CHEN Shan-yu REN Rui WANG Da-gang yang Zhong-lu ZHI Hai-jian National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement Nanjing Agricultural University

Soybean mosaic virus （SMV） disease is one of the most destructive viral diseases in soybean （Glycine max （L.） Merr.）. SMV strain SC3 is the major prevalent strain in huang-huai and yangtze valleys, China. The soybean cultivar Qihuang 1 is of a rich resistance spectrum and has a wide range of application in breeding programs in China. In this study, F1, F2 and F2：3 from Qihuang 1×nannong 1138-2 were used to study inheritance and linkage mapping of the SC3 resistance gene in Qihuang 1. The secondary F2 population and near isogenic lines （nILs） derived from residual heterozygous lines （RhLs） of Qihuang 1×nannong 1138-2 were separatively used in the ifne mapping and candidate gene analysis of the resistance gene. Results indicated that a single dominant gene （designated RSC3Q） controls resistance, which was located on chromosome 13. Two genomic-simple sequence repeat （SSR） markers BARCSOYSSR_13_1114 and BARCSOYSSR_13_1136 were found lfanking the two sides of the RSC3Q. The interval between the two markers was 651 kb. Quantitative real-time PCR analysis of the candidate genes showed that ifve genes （Glyma13g25730, 25750, 25950, 25970 and 26000） were likely involved in soybean SMV resistance. These results would have utility in cloning of RSC3Q resistance candidate gene and marker-assisted selection （MaS） in resistance breeding to SMV.

关键词： Soybean mosaic virus (SMV) resistance gene ifne mapping residual heterozygous line (RHLs) near isogenic lines (nILs) qRT-PCR

Transarterial injection of H101 in combination with chemoembolization overcomes recurrent hepatocellular carcinoma

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World Journal of Gastroenterology 2011年第18期17卷 2353-2355页

作者： qing He yang Liu qing Zou yong-Song Guan Department of Oncology West China Hospital of Sichuan University Chengdu 610041 Sichuan Province China

Transcatheter arterial chemoembolization (TACE) has become the standard treatment modality for unresectable hepatocellular carcinoma (HCC). Nonetheless, the clinical outcomes in patients with unresectable HCC are often unsatisfactory, especially in those with recurrent HCC. H101, an E1B gene deleted adenovirus, is known to have a significant antitumor activity. In addition, local injection of H101 can enhance the effect of antitumor therapies (chemotherapy and radiotherapy). Transarterial H101 gene injection in combination with TACE may help to control refractory and recurrent HCC. In this study, we report a 55-year-old patient with recurrent HCC which was treated with transarterial injection of H101 in combination with TACE, leading to a good clinical prognosis of the patient.

关键词： Hepatocellular carcinoma H101 Transcatheter arterial chemoembolization Therapy

Influence of Chromatin 3D Organization on Structural Variations of the Arabidopsis thaliana Genome

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Molecular Plant 2017年第2期10卷 340-344页

作者： Tao, Jing-Fen Zhou, Jin-Zhi Xie, Ting Wang, Xiao-Tao yang, qing-yong Zhang, Hong-Yu Huazhong Agr Univ Hubei Key Lab Agr Bioinformat Coll Informat Wuhan 430070 Hubei Peoples R China Huazhong Agr Univ Natl Key Lab Crop Genet Improvement Wuhan 430070 Hubei Peoples R China

Dear Editor The spectrum of genetic variations ranges from single nucleotide polymorphisms （SNPs） to large-scale structural variants （SVs）. Genome SVs involving kilobase- to megabase-sized insertions, deletions, duplications, and inversions, as well as complex com- binations of rearrangements, are common during evolution in various species （Feuk et al., 2006; Saxena et al., 2014）. A larger fraction of the human genome is affected by SVs than SNPs,

关键词： Chromatin Structural Genome

Changes of Plasma Membrane H^+-ATPase Activities of Glycine max Seeds by PEG Treatment

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Forestry Studies in China 2005年第2期7卷 7-11页

作者： yang yong-qing Wang Xiao-feng College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 P. R. China

The soybean （Glycine max） Heihe No. 23 is sensitive to imbibitional chilling injury. Polyethylene glycol （PEG） treatment can improve chilling tolerance of soybean seeds to a certain extent. The changes of hydrolytic ATPase in plasma membranes and H^＋-pumping responses in soybean seeds were investigated during PEG treatments. Effects of exogenous calcium and exogenous ABA on the hydrolytic ATPase were also examined in order to understand the mechanism of chilling resistance. Highly purified plasma membranes were isolated by 6.0% aqueous two-phase partitioning from soybean seeds, as judged by the sensitivity of hydrolytic ATPase to sodium vanadate. PEG treatment resulted in a slight increase of the hydrolytic ATPase activity in 12 h. Then the activity decreased gradually, but still higher than the control. The H^＋-pumping activity increased steadily during PEG treatment. Exogenous calcium had both activating and inhibiting effects on the hydrolytic ATPase, but the activity was inhibited in soybean seeds treated with exogenous ABA. Results suggested that PEG treatment, not the exogenous calcium and ABA, up-regulated H^＋-ATPase activities in soybean seeds.

关键词： soybean seeds PEG treatment plasma membrane (PM) H^+-ATPase exogenous calcium exogenous ABA

Association mapping of lignin response to Verticillium wilt through an eight-way MAGIC population in Upland cotton

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Journal of Integrative Agriculture 2023年第5期22卷 1324-1337页

作者： TIAN Xiao-min HAN Peng WANG Jing SHAO Pan-xia AN Qiu-shuang Nurimanguli AINI yang qing-yong YOU Chun-yuan LIN Hai-rong ZHU Long-fu PAN Zhen-yuan NIE Xin-hui Key Laboratory of Oasis Eco-agricultural Xinjiang Production and Construction Corps/Agricultural CollegeShihezi UniversityShihezi 832003P.R.China College of Informatics Huazhong Agricultural UniversityWuhan 430070P.R.China Cotton Research Institute Shihezi Academy of Agricultural SciencesShihezi 832011P.R.China National Key Laboratory of Crop Genetic Improvement/College of Plant Sciences&Technology Huazhong Agricultural UniversityWuhan 430070P.R.China

Lignin metabolism plays a pivotal role in plant defense against pathogens and is always positively correlated as a response to pathogen infection. Thus, understanding resistance genes against plant pathogens depends on a genetic analysis of the lignin response. This study used eight Upland cotton lines to construct a multi-parent advanced generation intercross(MAGIC) population(n=280), which exhibited peculiar characteristics from the convergence of various alleles coding for advantageous traits. In order to measure the lignin response to Verticillium wilt(LRVW), the artificial disease nursery(ADN) and rotation nursery(RN) were prepared for MAGIC population planting in four environments. The stem lignin contents were collected, and the LRVW was measured with the lignin value of ADN/RN in each environment, which showed significant variations. We employed 9 323 high-quality single-nucleotide polymorphism(SNP) markers obtained from the Cotton-SNP63K array for genotyping the MAGIC population. The SNPs were distributed through the whole genome with 4.78 SNP/Mb density, ranging from 1.14(ChrA06) to 10.08(ChrD08). In addition, a genome-wide association study was performed using a Mixed Linear Model(MLM) for LRVW. Three stable quantitative trait loci(QTLs), qLRVW-A04, qLRVW-A10, and qLRVW-D05, were identified in more than two environments. Two key candidate genes, Ghi_D05G01046 and Ghi_D05G01221, were selected within the QTLs through the combination of variations in the coding sequence, induced expression patterns, and function annotations. Both genes presented nonsynonymous mutations in coding regions and were strongly induced by Verticillium dahliae. Ghi_D05G01046 encodes a leucine-rich extensin(LRx) protein involved in Arabidopsis cell wall biosynthesis and organization. Ghi_D05G01221 encodes a transcriptional co-repressor novel interactor of novel interactor of jasmonic acid ZIM-domain(JAZ–NINJA), which functions in the jasmonic acid(JA) signaling pathway. In summary, t

关键词： genome-wide association study lignin response MAGIC population Upland cotton Verticillium wilt

Comparison Study on Three Different Reconfigurable Optical Add Drop Multiplexer Architectures

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Semiconductor Photonics and Technology 2007年第4期13卷 268-271页

作者： ZHOU Bin ZHANG yang-an HUANG yong-qing REN Xiao-min Key Laboratory of Optical Communication and Lightwave Technologies Ministry of Education Beijing University of Posts and Telecommunications Beijing 100876 CHN

In this paper, we present the evolution of reconfigurable optical add drop multiplexer（ROADM） technologies, and compare three main ROADM architectures available on the market today. Three archhectures include broadcast-select and demux-switch-mux[and the integrated version planar lightwave circuit（PLC）] and wavelengths-selective switch.

关键词： ROADM broadcast-and-select demux-switeh-mux PLC wavelengths-selective switch

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大黄酸对大鼠前体脂肪细胞增殖与分化的影响

中国药学杂志 2007年第15期42卷 1148-1151页

作者：杨永青杨公社西北农林科技大学动物脂肪沉积与肌肉发育实验室

目的研究大黄酸（rhein，Rh）对大鼠前体脂肪细胞增殖与分化的影响。方法采用噻唑蓝（MTT）比色法和流式细胞术测定RH对大鼠前体脂肪细胞增殖的影响。采用油红O染色提取法，检测脂肪细胞内甘油三酯积聚量的变化；采用形态学观察，检... 详细信息

目的研究大黄酸（rhein，Rh）对大鼠前体脂肪细胞增殖与分化的影响。方法采用噻唑蓝（MTT）比色法和流式细胞术测定RH对大鼠前体脂肪细胞增殖的影响。采用油红O染色提取法，检测脂肪细胞内甘油三酯积聚量的变化；采用形态学观察，检测前体脂肪细胞充脂率、充脂程度及形态变化。结果 RH在5-160μmol·L^-1内，对前体脂肪细胞增殖与分化的抑制作用呈剂量和时间依赖性变化，并可在一定程度上诱发脂肪细胞凋亡。结论 Rh可抑制前体脂肪细胞的增殖与分化，具有潜在的减肥降脂作用。

关键词：大黄酸前体脂肪细胞增殖与分化凋亡