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Time-resolved ESI-MS Investigation on Reduction and Alkylation in Unfolding Process of Lysozyme

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Chemical Research in Chinese Universities 2001年第4期17卷 387-393页

作者： SONG Hao-wei XU Chong-feng FU Yun-lin yang peng-yuan WANG Hong-hai Department of Chemistry State Key Laboratory of Genetic Engineering Fudan UniversityShanghai 200433P.R.China

The kinetic procedure of the unfolding of lysozyme induced by the reduction of disulfide was monitored by the time-resolved ESI-MS with a sheath liquid assistant electrospray interface. It was found that the reduction process for the eight disulfides had a less difference in the reaction time after denatured treatment. In addition, the alkylation of the reduced free thiols was much slower than the reduction procedure. An artifact peak produced by the CID fragmentation in the mass spectra was identified and the possible mechanism of the Hofmann elimination reaction was proposed.

关键词： Unfolding Lysozyme Reduction and alkylation Time-resolved ESI-MS

Non-decoloured In-gel Digestion of Coomassie Blue-stained Proteins

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Chemical Research in Chinese Universities 2003年第4期19卷 399-403页

作者： ZHANG Guo-an BAO Hui-min FAN Hui-zhi yang peng-yuan Department of Chemistry Fudan UniversityShanghai 200433P.R.China

A simplified method is presented for tryptic digestion of Coomassie brilliant blue(CBB)-stained proteins in polyacrylamide gels. Compared with conventional methods, the proposed method does not require a removal of the dye before digestion, and is thus faster and saves a lot of labor. The resulted digest can be analyzed by either RPLC/ESIMS or MALDI MS for identification of the protein in a conventional way. Model studies with bovine serum albumin(BSA) showed that 50 ng of the protein could be routinely identified. The simplified procedure displays a tendency to produce more incompletely cleaved peptides, which is favorable for improving the sequence coverage.

关键词： In-gel digestion Polyacrylamide gel electrophoresis Coomassie brilliant blue Mass spectrometry

Electronic Noses Using Quantitative Artificial Neural Network

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Chemical Research in Chinese Universities 2001年第4期17卷 380-386页

作者： WEN Li-jing BIAN Li-ping LU Yu ZHANG Mei-zhuo YU Li-ping yang peng-yuan Department of Chemistry Fudan UniversityShanghai 200433P.R.China

The present paper covers a new type of electronic nose (e-nose) with a four-sensor array, which has been applied to detecting gases quantitatively in the presence of interference. This e-nose has adapted fundamental aspects of relative error (RE) in changing quantitative analysis into the artificial neural network (ANN). Thus, both the quantitative and the qualitative requirements for ANN in implementing e-nose can be satisfied. In addition, the e-nose uses only 4 sensors in the sensor array, and can be designed for different usages simply by changing one or two sensor(s). Various gases were tested by this kind of e-nose, including alcohol vapor, CO, iiquefied-petrol-gas and CO2. Satisfactory quantitative results were obtained and no qualitative mistake in prediction was observed for the samples being mixed with interference gases.

关键词： E-nose ANN Relative error Quantitative analysis

Preparation of A New Type of Stress-absorbed Material

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Journal of Wuhan University of Technology(Materials Science) 2004年第4期19卷 76-78页

作者：吴少鹏 Wuhan University of Technology

Neoprene latex modified emulsified bitumen and fine aggregate are used to prepare a new type of stress-absorbed material, which has strong ability of anti-reflective cracking on asphalt concrete over layer-constructed upon a semi-rigid type base course or cement concrete pavement block. Experimental results demonstrate the stress-absorbed material have excellent mechanical properties including a low modulus of elasticity, high ultimate tensile stress and strain, and a strong distortion ability. Stress concentration in asphalt over layer originated by temperature changes and traffic loads can be alleviated.

关键词： reflective cracking stress-absorbed emulsified bitumen

Inductively Coupled Plasma(ICP) Mass Spectrometry(MS) Hyphenated with Atomic Emission Spectrometry(AES) for Simultaneous Determination of Major, Minor and Micro Amounts of Elements in Geochemical Samples

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Chemical Research in Chinese Universities 2005年第6期21卷 753-756页

作者： HUANG Zhen-yu ZHANG Qin HU Ke WU Jian-ling yang peng-yuan Department of Chemistry Fudan University Shanghai 200433 P. R. China Academy of Chinese Geology Science Langfang 065000 P. R. China Lab Tech Beijing 101312 P. R. China Institute of Chemistry and Chemical Engineering Guangxi University Nanning 530004 P. R. China

Introduction Geological resource survey demands for determining various constituents including major, minor, micro, trace and ultra-trace levels of elements for preparing the map of resource distribution of our country. As a powerful and popularly used technique for multi-element analysis, inductively coupled plasma（ICP） atomic emission spectrometry （AES） has been applied to this field for a period of time. However, ICP spectrometric determination of those micro, trace and ultratrace elements needs enrichment procedures for improving the detection limit, which is unacceptable in case a great mass of samples should be analyzed as that in the task of geological resource survey. On the other hand, although ICP mass spectrometry （MS） is considered the most powerful method for trace elements determination, it is difficult for ICP-MS to be used to determine the trace and major analytes simultaneously in a spectrum.

关键词： ICP-MS ICP-AES Hyphenated method Geochemistry sample

Detection　of　Combustible　Gases　by　Semiconductor　Sensors──Rapid　Separation　of　Hydrogen　Sulphide　with　β，β＇－oxydipropionitrile

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Chemical Research in Chinese Universities 1996年第4期12卷 401-406页

作者： yang Yan-ming WANG Xiao-ru yang peng-yuan and CAI Lu-xin(Department of Chemistry,The Research Laboratory of SEDC of ASM&LC,Xiamen University,Xiamen,361005) XIAMEN UNIV DEPT CHEMRES LAB SEDC ASM & LCXIAMEN 361005PEOPLES R CHINA

ＤｅｔｅｃｔｉｏｎｏｆＣｏｍｂｕｓｔｉｂｌｅＧａｓｅｓｂｙＳｅｍｉｃｏｎｄｕｃｔｏｒＳｅｎｓｏｒｓ──ＲａｐｉｄＳｅｐａｒａｔｉｏｎｏｆＨｙｄｒｏｇｅｎＳｕｌｐｈｉｄｅｗｉｔｈβ，β＇－ｏｘｙｄｉｐｒｏｐｉｏｎｉｔｒ... 详细信息

ＤｅｔｅｃｔｉｏｎｏｆＣｏｍｂｕｓｔｉｂｌｅＧａｓｅｓｂｙＳｅｍｉｃｏｎｄｕｃｔｏｒＳｅｎｓｏｒｓ──ＲａｐｉｄＳｅｐａｒａｔｉｏｎｏｆＨｙｄｒｏｇｅｎＳｕｌｐｈｉｄｅｗｉｔｈβ，β＇－ｏｘｙｄｉｐｒｏｐｉｏｎｉｔｒｉｌｅＹＡＮＧＹａｎ－ｍｉｎｇ；Ｗ...

关键词： s:Hydrocarbon Hydrogen sulphide Rapid separation

液相等电聚焦结合乙酰化标记在比较蛋白质组学中的应用

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分析化学 2008年第4期36卷 449-453页

作者：李娜杨芃原复旦大学化学系复旦大学化学系上海200433

建立了液相等电聚焦,一维电泳分离以及肽段乙酰化同位素标记的新技术,此技术在1∶5;∶1范围内具有较好的动态范围（误差小于20%）。本方法不仅可以提高比较蛋白质组分离的通量,并有助于低丰度差异蛋白中的检出;并成功应用于正常肝脏与... 详细信息

建立了液相等电聚焦,一维电泳分离以及肽段乙酰化同位素标记的新技术,此技术在1∶5;∶1范围内具有较好的动态范围（误差小于20%）。本方法不仅可以提高比较蛋白质组分离的通量,并有助于低丰度差异蛋白中的检出;并成功应用于正常肝脏与癌变肝脏组织的低丰度、偏碱性蛋白的差异比较蛋白质组分析,鉴定出16种差异蛋白,结果令人满意。

关键词：液相等电聚焦(LIEF) 一维凝胶电泳乙酰化标记蛋白质组学

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液相等电聚焦结合双向凝胶电泳分离碱性蛋白

高等学校化学学报 2006年第10期27卷 1830-1834页

作者：钟华张扬樊惠芝杨芃原复旦大学生物医学研究院蛋白质组学研究中心上海200433

在蛋白组学研究中,经典的双向凝胶电泳法(2-DE)对碱性蛋白及低丰度蛋白的分离存在技术障碍,但预分离技术的应用可弥补其缺陷.液相等电聚焦可有效地分离富集复杂蛋白样品.碱性胶条用于2-DE可极大地提高蛋白上样量和凝胶分辨率.将上述两... 详细信息

在蛋白组学研究中,经典的双向凝胶电泳法(2-DE)对碱性蛋白及低丰度蛋白的分离存在技术障碍,但预分离技术的应用可弥补其缺陷.液相等电聚焦可有效地分离富集复杂蛋白样品.碱性胶条用于2-DE可极大地提高蛋白上样量和凝胶分辨率.将上述两种技术相结合用于碱性蛋白质和低丰度蛋白质的分离鉴定,可使碱端区域双向凝胶图谱质量显著提高,蛋白点更清晰且点数增多,质谱鉴定确信度提高,碱性蛋白和低丰度蛋白质谱鉴定成功率提高,对于蛋白组学研究具有一定的意义.

关键词：液相等电聚焦双向凝胶电泳碱性蛋白

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糖蛋白质组学的信息学资源和方法

生物化学与生物物理进展 2016年第9期43卷 910-918页

作者：刘杭姚鋆杨芃原张扬复旦大学医学院生物医学研究院上海200032

糖基化修饰是生物体内最常见、最重要的蛋白质翻译后修饰之一.哺乳动物体内超过50%的蛋白质都会发生糖基化修饰.糖蛋白广泛分布于各种组织的细胞膜表面,执行着重要的生物学功能.随着高通量、高灵敏度和高分辨率的蛋白质组学时代的来临,... 详细信息

糖基化修饰是生物体内最常见、最重要的蛋白质翻译后修饰之一.哺乳动物体内超过50%的蛋白质都会发生糖基化修饰.糖蛋白广泛分布于各种组织的细胞膜表面,执行着重要的生物学功能.随着高通量、高灵敏度和高分辨率的蛋白质组学时代的来临,许多基于串级质谱技术解析糖链结构的生物数据库和分析软件也亦应运而生.本文综述了目前文献中最常用的糖类生物信息学资源,包括各种糖蛋白的数据库以及质谱解析糖类的相关工具和新技术、新方法.

关键词：糖蛋白质组学质谱糖信息学数据库软件