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贵州烟区产业综合体发展模式研究

园艺与种苗 2024年第4期44卷 108-110页

作者：杨丽梅遵义市播州区种植业发展服务中心贵州遵义563100

为了稳定烟区发展,提升烟农收入,推动烟草产业与现代农业的有机结合,探索烟区产业综合体高质量发展,文章概述了烟区产业综合体的含义及发展特征,深入分析了贵州省现有的农业内部融合型、农业延伸拓展型和新技术渗透型3种烟区产业综合体... 详细信息

为了稳定烟区发展,提升烟农收入,推动烟草产业与现代农业的有机结合,探索烟区产业综合体高质量发展,文章概述了烟区产业综合体的含义及发展特征,深入分析了贵州省现有的农业内部融合型、农业延伸拓展型和新技术渗透型3种烟区产业综合体的发展模式。贵州省3种烟区产业综合体为其他地区烟草产业的发展提供了可供借鉴、可供复制的经验,各地在建设和发展烟区产业综合体时要注意把握相关要点,结合实际选择烟草产业的可持续发展之路。

关键词：贵州省烟区综合体发展模式

面向车流量智能检测的YOLOv7算法改进与应用

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公路 2024年第1期69卷 242-249页

作者：马庆禄吴跃川张梓轩李杨梅重庆交通大学交通运输学院重庆市400074 宁夏交投高速公路管理有限公司银川市750000

针对当前机器视觉识别中车流量检测的精度问题,以YOLOv7人工智能算法为基础,通过视觉跟踪并叠加注意力机制,提出一种基于YOLOv7和Deep SORT的改进型车流量智能检测方法。通过将注意力模块GAM与YOLOv7网络进行融合增强检测网络的特征提... 详细信息

针对当前机器视觉识别中车流量检测的精度问题,以YOLOv7人工智能算法为基础,通过视觉跟踪并叠加注意力机制,提出一种基于YOLOv7和Deep SORT的改进型车流量智能检测方法。通过将注意力模块GAM与YOLOv7网络进行融合增强检测网络的特征提取能力;同时在改进后的YOLOv7网络中引入Deep SORT跟踪算法以改善车辆间相互遮挡导致复检漏检问题。实验选取重庆市渝中区经纬大道双向六车道为研究对象,在新铺社天桥上采用固定相机连接移动笔记本电脑的方式进行数据采集以及算法验证,为了保证算法的可重复性,分别选取早高峰、午平峰和晚高峰3个时段分别录取了5 min的交通流视频。利用在交通视频中通过设置虚拟检测线,让新算法在车辆检测的同时对车辆运行轨迹进行跟踪,当车辆经过检测线时记录车辆的身份编号,以此来实现交通视频的车流量监测与跟踪计数。实验结果表明:改进后的新算法相比于原YOLOv7算法在车辆检测方面平均精度提高了2.3%,视频车流量统计的精度提高了8.2%。

关键词：智能交通车流量检测 YOLOv7 Deep SORT 深度学习

Preparation and application of efficient TiO_2 /ACFs photocatalyst

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Journal of Environmental Sciences 2006年第5期18卷 979-982页

作者： liU Jian-hua yang Rong li Song-mei School of Materials Science and Engineering Beihang University Beijing 100083 China

Activated carbon fibers （ACFs） supported titanium dioxide （TiO2） photocatalyst was developed by sol-gel method. The surface morphology and microstructure of the photocatalyst were characterized with scan electron microscope（SEM）, X-ray diffraction patterns and specific surface area analysis. The prepared photocatalyst is specially helpful for the removal of low molecular weight organic pollutants in wastewater. Decomposition efficiency of methylene blue solution by TiO2/ACFs catalyst reached almost 100% under 60 min reaction, while the decomposition efficiency by pure TiO2 was only 25% under 3 h reaction. The mineralization of toluene aqueous solution was measured by total organic carbon instrument, and the evolution of intermediate species was detected by gas chromatograph instrument. The results indicated that the prepared photocatalyst not only enhanced the photoactivity of TiO2, but also suppressed the emergence of intermediate species, which may be more deleterious to human. The enhancement of photocatalysis was due to increased efficiency of adsorption and desorption, which were control steps in heterogeneous photocatalysis.

关键词： titanium dioxide activated carbon fibers photocatalysis toluene methylene blue

Transformation of Cabbage(Brassica oleracea ***) with Bt cry1Ba3 Gene for Control of Diamondback Moth

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Agricultural Sciences in China 2011年第11期10卷 1693-1700页

作者： YI Deng-xia CUI Lei liU Yu-mei ZHUANG Mu ZHANG yang-yong FANG Zhi-yuan yang li-mei Key Laboratory of Horticultural Crops Genetic Improvement Ministry of Agriculture~Institute of Vegetables and Flowers Chinese Academyof Agricultural Sciences Beijing 100081 P.R.China

To obtain transgenic cabbage line with broad insect resistance, a new synthetic Bacillus thuringiensis crylBa3 gene was introduced into white cabbage via Agrobacterium tumefaciens-mediated transformation and 37 transformants were obtained. Polymerase chain reaction （PCR） and Southern blot analyses confirmed that crylBa3 was successfully inserted into the genome of cabbage. Reverse transcription-polymerase chain reaction （RT-PCR） demonstrated that crylBa3 was expressed. Western blot results confirmed the production of insecticidal protein encoded by crylBa3. Insect bioassays showed that transgenic cabbages effectively controlled both susceptible and CrylAc-resistant diamondback moth （DBM） larvae.

关键词： Bacillus thuringiensis crylBa3 cabbage diamondback moth resistance

Induces Resistance Against Fusarium and Pink Rots by Acibenzolar-S-Methyl in Harvested Muskmelon (***)

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Agricultural Sciences in China 2008年第1期7卷 58-64页

作者： GE Yong-hong BI yang li Xuan li mei College of Food Science and Engineering Gansu Agricultural University Lanzhou 730070 P.R.China

Acibenzolar-S-methyl （ASM） is a chemical activator of systematic resistance in many plants. The effect of preharvest and postharvest application of ASM was evaluated for its ability to induce resistance in muskmelon fruit. The results indicated that 50 and 100 mg L^-1 ASM or 1 mL L^-1 imazalil at 1 week or 1 day before harvest were effective in reducing the lesion area with 100 mg L^-1 ASM the most effective. No treatment inhibited the infection rate. The postharvest results showed that 50 and 100 mg L^-1 ASM, and 0.1 mL L^-1 imazalil were effective in reducing the lesion area with 100 mg L^-1 ASM the most effective. No treatment inhibited the infection rate. There was a clear time-dependent response of the fruit to postharvest ASM treatment, in which treatments applied 1, 3, and 5 day before inoculation provided the best results. ASM did not demonstrate any fungicide effect in vitro and suppressed lesion area in treated muskmelons, indicating that disease resistance was induced. The protection of ASM was associated with the activation of peroxidase （POD） in treated muskmelons.

关键词： acibenzolar-S-methyl (ASM) induced resistance muskmelon peroxidase (POD)

灰树花多糖纳米乳的制备及其对H9亚型禽流感病毒与常见细菌抑制效果的评估

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中国预防兽医学报 2024年第8期46卷 856-862页

作者：刘博毕研丽杨丽梅冯宗玲李佳苏海歌孙红武张艳王文秀海南省农业科学院畜牧兽医研究所海南省热带动物繁育与疫病研究重点实验室海南海口571100 山东省滨州畜牧兽医研究院山东滨州256600 山东绿都生物科技有限公司山东滨州256600 滨州市科技创新发展研究院山东滨州256600 中国人民解放军陆军军医大学重庆400038 滨州点石成金技术转移与成果转化服务中心山东滨州256600

为制备灰树花多糖(GFP)纳米乳,并观察其理化及形态学特征,评估其对H9亚型禽流感病毒(AIV)及3种常见细菌的抑制效果,本研究采用低能乳化法制备GFP纳米乳,经不同方式处理后观察GFP纳米乳的稳定性,分别采用透射电镜和扫描电镜观察其形态特... 详细信息

为制备灰树花多糖(GFP)纳米乳,并观察其理化及形态学特征,评估其对H9亚型禽流感病毒(AIV)及3种常见细菌的抑制效果,本研究采用低能乳化法制备GFP纳米乳,经不同方式处理后观察GFP纳米乳的稳定性,分别采用透射电镜和扫描电镜观察其形态特征,使用激光粒度仪测定GFP纳米乳的粒径与多分散系数(PDI),使用电位仪测定其Zeta电位。结果显示:该GFP纳米乳外观澄清、透明,8000 r/min离心20 min和室温静止7 d后不分层、不破乳,表明制备的GFP纳米乳性状稳定。电镜观察可见许多单一分布的均匀圆球,其周围包裹着网格状凝胶基质;GFP纳米乳的粒径为10 nm~100 nm,平均粒径24.12 nm,PDI为0.096,Zeta电位为-18.6±4.3 m V,表明GFP纳米乳稳定性较好。将不同浓度的GFP纳米乳及免手洗凝胶消毒剂分别于MDCK细胞中与H9亚型AIV NJ02株作用,通过计算各药物对H9亚型AIV的抑制率比较GFP纳米乳对H9亚型AIV的抑制效果。将不同稀释度的GFP纳米乳、GFP溶液及免手洗凝胶和75%乙醇分别与AIV NJ02株作用后,采用荧光定量PCR(q PCR)检测H9亚型AIV基因的拷贝数,并计算各药物对H9亚型AIV的抑制率。采用药敏纸片法检测GFP纳米乳、GFP原液、免手洗凝胶对禽舍3种常见细菌的抑菌效果。对H9亚型AIV的抑制率检测结果显示,当浓度低于10μL/mL时,GFP纳米乳及免手洗凝胶消毒剂对H9亚型AIV的抑制率均小于55%,效果不明显;当浓度大于40μL/mL时,GFP纳米乳对H9亚型AIV的抑制率极显著高于同浓度的免手洗凝胶(P<0.01);当浓度为80μL/mL时,GFP纳米乳可以100%抑制病毒增殖,且对细胞无毒性。qPCR结果显示,10倍稀释的各药物对H9亚型AIV的抑制率均明显高于100倍稀释的各药物,且10倍稀释的GFP纳米乳对H9亚型AIV的抑制率均极显著高于免手洗凝胶、GFP原液和75%乙醇(P<0.01)。纸片扩散法结果显示,GFP纳米乳对金黄色葡萄球菌的抑菌效果较差,对枯草芽孢杆菌和大肠杆菌的抑菌效果较强,但总体GFP纳米乳的抑菌效果比免手洗凝胶稍差。上述结果表明,本研究制备的GFP纳米乳性状稳定,对体外培养的H9亚型AIV具有良好的抑制效果,对禽舍常见细菌具有一定的抑制效果,且安全无毒副作用,有望作为安全高效的纳米消毒剂用于畜禽和人类的消毒。本研究为开发新型抗病毒中草药纳米消毒剂奠定了实验基础。

关键词：灰树花多糖纳米乳 H9亚型禽流感病毒抑菌效果

Application of extended Kalman filtering on aircraft pose prediction of image sequences

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Optoelectronics Letters 2007年第1期3卷 69-72页

作者： yang li-mei GUO li-hong Changchun Institute of Optics Fine Mechanics and Physics Chinese Academy of Sciences Changchun 130033 China Graduate School of the Chinese Academy of Sciences Beijing 100039

In allusion to the character of monocular image sequences, a method based on extended Kalman filtering to predict the aircraft pose of image sequences is proposed. With α - β - γ stable state filtering technique, a mathematics model is built to realize the prediction of aircraft pose of image sequences. In the model, not only the influence of noise during the image process is considered, but also the shortcoming of low precision in the constant velocity model is overcomed. The derivation of acceleration is considered as white noise. The predictive curve plotted with Matlab proves that the maximum of error of using this method is about 3’. So its precision is higher and error standard deviation is lower than those of the constant velocity model.

关键词：图象序列飞行器姿态预测扩展卡尔曼滤波应用

A Co-Dominant Marker BoE332 Applied to Marker-Assisted Selection of Homozygous Male-Sterile Plants in Cabbage (Brassica oleracea *** L.)

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Journal of Integrative Agriculture 2013年第4期12卷 596-602页

作者： CHEN Chen ZHUANG Mu FANG Zhi-yuan WANG Qing-biao ZHANG yang-yong liU Yu-mei yang li-mei CHENG Fei Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Horticulture College of Qingdao Agricultural University

The dominant genic male sterility （DGMS） gene CDMs399-3 derived from a spontaneous mutation in the line 79-399-3 of spring cabbage （Brassica oleracea var. capitata L.）, has been successfully applied in hybrid seed production of several cabbage cultivars in China. During the development of dominant male sterility lines in cabbage, the conventional identification of homozygous male-sterile plants （CDMs399-3/CDMs399-3） is a laborious and time-consuming process. For marker-assisted selection （MAS） of the gene CDMs399-3 transferred into key spring cabbage line 397, expressed sequence tag-simple sequence repeats （EST-SSR） and SSR technology were used to identify markers that were linked to CDMs399-3 based on method of bulked segregant analysis （BSA）. By screening a set of 978 EST-SSRs and 395 SSRs, a marker BoE332 linked to the CDMs399-3 at a distance of 3.6 cM in the genetic background of cabbage line 397 were identified. 7 homozygons male-sterile plants in population P1170 with 20 plants were obtained finally via MAS of BoE332. Thus, BoE332 will greatly facilitate the transferring of the gene CDMs399-3 into the key spring cabbage line 397 and improve the application of DGMS in cabbage hybrid breeding.

关键词： cabbage (Brassica oleracea var. capitata L.) dominant genic male sterility (DGMS) expressed sequencetag-simple sequence repeats (EST-SSR) bulked segregant analysis (BSA) marker-assisted selection (MAS)

Effects of kallikrein gene transfer on penumbral microvascular proliferation and on regional cerebral blood flow following cerebral ischemia/reperfusion injury

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Neural Regeneration Research 2008年第10期3卷 1045-1050页

作者： Ruiyan Lu Qingyu Shen lianhong yang mei li Yidong Wang Ying Peng Department of Neurology Second Affiliated Hospital of Sun Yat-sen University Guangzhou 510120 Guangdong Province China

BACKGROUND： Recent findings have demonstrated that the kallikrein-kinin system （KKS） participates in the pathological process of cerebral ischemia/reperfusion injury. Kallikrein gene transfer exhibits neural protective effects following cerebral infarction. OBJECTIVE： To observe the effects of kallikrein gene transfer on vascular proliferation in the peripheral infarct focus and on regional cerebral blood flow （rCBF） following cerebral ischemia/reperfusion injury. DESIGN, TIME AND SETTING： The completely randomized, controlled experiment was performed at the lin Baixin Laboratory Center, the Second Affiliated Hospital of Sun Yat-sun University between September 2007 and April 2008. MATERIALS： pUCI9-HTK plasmid was constructed and maintained in the Laboratory for Neurology, the Second Affiliated Hospital of Sun Yat-sen University, China. Mouse anti-human kallikrein 1 monoclonal antibody was purchased from R＆D Systems, USA. METHODS Ninety healthy, male, Sprague Dawley rats were used. Middle cerebral artery occlusion （MCAO） was established in all rats to induce cerebral ischemia/reperfusion injury. Following MCAO establishment, all rats were randomly divided into three groups （n = 30）： blank control, saline, and pAdCMV-HTK. The saline and pAdCMV-HTK groups were stereotactically micro-injected with 5μL of physiological saline or with pAdCMV-HTK [multiplicity of infection （MOI） = 20], respectively, into the ischemic penumbra. In the blank control group, only sham injection was performed. MAIN OUTCOME MEASURES： At 12, 24, and 72 hours after treatment, cerebral infarction volume was measured by 2, 3, 5-triphenyltetrazolium chloride （TTC） staining. Exogenous HTK expression, as well as regional vascular endothelial growth factor （VEGF） expression, was detected by immunohistochemistry. rCBF was examined by 14C-iodoantipyrine micro tracing. In addition, neurological severity score （NSS） was performed. Higher scores indicated more severe neurological deficits. RESULTS： NSS resu

关键词： cerebral ischemia ischemia/reperfusion injury adenovirus gene transfer kallikrein vascular endothelial growth factor perfusion, regional