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Soil Phosphorus Distribution as Affected by Irrigation Methods in Plastic Film House

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Pedosphere 2011年第6期21卷 712-718页

作者： yang li-juan ZHANG Yu-Long li Fu-Sheng J. H. LEMCOFF College of Land Recourses and Environmental Science Shenyang Agricultural University Shenyang 110161 （China） College of Agriculture Guangxi University Nanning 530005 （China） Institute of Soil Water and Environmental Sciences Volcani Center Bet Dagan 50250 （Israel）

Water-saving irrigation methods have been increasingly used for vegetable cultivation in greenhouse or plastic film house. However, there is limited information concerning the effect of water-saving irrigation methods on soil phosphorus （P） behavior. In this experiment, drip and subsurface irrigation methods were applied, with furrow irrigation method as control, in Mollic Gleysols. Soil P distribution throughout the depth was significantly affected by irrigation methods. Total, Olsen, organic and inorganic P contents were greater in the topsoil （0-10 and 10-20 cm） than in the subsoil （20-30, 30-40, 40-50 and 50-60 cm）. The Olsen P content throughout 0-60 cm layer under drip and subsurface irrigation treatments was lower than that under the furrow irrigation treatment. However, the total, organic and inorganic P contents from 20 to 60 cm under drip irrigation treatment were higher than or close to those under furrow irrigation treatment, but were lower under subsurface irrigation treatment than under furrow irrigation treatment. Under subsurface irrigation treatment, the contents of total, organic and inorganic P at the 0-10 cm layer were 78.0%, 1.3% and 3.7% greater than those at the 10-20 cm layer, respectively. But Olsen P content at the 10-20 cm layer was 5.7% larger than that at the 0-10 cm layer. These suggested that soil P behavior could be manipulated by soil water management to some extent. 在

关键词： drip irrigation furrow irrigation soil P forms subsurface irrigation tomato

A PEER-TO-PEER INCENTIVE SCHEME FOR OVERCOMING FREE RIDING

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Journal of Electronics(China) 2010年第1期27卷 60-67页

作者： Tian Junfeng yang lidan li juan Tian Rui Network Technology Institute Mathematics and Computer College Hebei University Baoding 071002 China

Free riding has a great influence on the expandability,robustness and availability of Peer-to-Peer(P2P) *** free riding has become a hot research issue both in academic and industrial *** incentive scheme is proposed to overcoming free riding in P2P network in this *** to the behavior and function of nodes,the P2P network is abstracted to be a Distributed and Monitoring-based Hierarchical Structure Mechanism(DMHSM) model.A utility function based on several influencing factors is defined to determine the contribution of peers to the whole *** paper also introduces reputation and permit mechanism into the scheme to guarantee the Quality of Service(QoS) and to reward or punish peers in the ***,the simulation results verify the effectiveness and feasibility of this model.

关键词： Peer-to-Peer(P2P) network Free riding Incentive mechanism Reputation Utility function

Biodegradation of Pentachloronitrobenzene by Labrys portucalensis pcnb-21 Isolated from Polluted Soil

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Pedosphere 2011年第1期21卷 31-36页

作者： li Rong ZHENG Jin-Wei NI Bin CHEN Kai yang Xiu-juan li Shun-Peng JIANG Jian-Dong Key Laboratory of Microbiological Engineering of Agricultural Environment Ministry of Agriculture College of Life Sciences Nanjing Agricultural University Nanjing 210095 (China)

A bacterial strain, pcnb-21, capable of degrading pentaehloronitrobenzene （PCNB） under aerobic and anoxic conditions, was isolated from a long-term PCNB-polluted soil by an enrichment culture technique and identified as Labrys portucalensis based upon its morphological, physiological and biochemical properties, as well as 16S rRNA gene sequence analysis. Effects of different factors, such as temperature and pH, on PCNB biodegradation were studied. Strain pcnb-21 efficiently degraded PCNB at temperatures from 20 to 30 ℃ and initial pH values from 4 to 7, which might be the first time that a Labrys strain was found capable of eflClciently degrading PC1NB. The degradation of PCNB was affected by oxygen, and the degradation decreased with increasing aeration. Exogenous electron donors such as glucose, lactic acid and succinic acid promoted the biodegradation of PCNB, while electron acceptors such as sodium nitrite, sodium sulfate, sodium nitrate and sodium sulfate inhibited PCNB biodegradation. The degradation of PCNB in sterile and non-sterile soils by a green fluorescent protein （GFP）-labeled strain, pcnb-21-gfp, was also studied. Cells of pcnb-21-gfp efficiently degraded 100 mg kg-1 PCNB in sterile and non-sterile soils and could not be detected after 42 days. Strain pcnb-21 might be useful in bioremediating PCNB-polluted soils and environment.

关键词： aeration bacterial strain electron acceptors electron donors sterile soil

Synthesis and Immunosuppressive Activity of New Amino Alcohols Derivatives(II)

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Chemical Research in Chinese Universities 2011年第5期27卷 808-812页

作者： CUI lin-lin MI Hao-yu ZHANG Qun-li WANG Wei-wei yang li-juan liANG Yong-tao WANG En-si College of life Science Jilin University Changchun 130012 t~ R. China College of Pharmacy Jilin University Changchun 130021 P. R. China

A series of new amino alcohols derivatives was synthesized with 6-bromo-2-tetralone as starting material, 6-substituted-2-tetralone as intermediate and Ullmann reaction, Suzuki reaction and spiro-hydantoin hydrolysis as key steps. The structures of key intermediate and target compounds were confirmed by 1H NMR, 13C NMR, IR and MS. Bioactivity researches show that the target compounds have certain inhibition effects on mice peripheral blood lymphocytes.

关键词： Immunosuppressant Amino alcohol derivative Bioactivity Buchere-Bergs reaction

Synthesis of Novel Benzoylphenylurea Chitin Inhibitors From Chlorothalonil(Ⅱ)

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Chemical Research in Chinese Universities 2004年第4期20卷 429-432页

作者： liN Jun MAO De-shou XU Rong YAN Sheng-jiao yang li-juan li Jun-feng liU Fu-chu Department of Applied Chemistry Yunnan UniversityKunming 650091P.R.China The Dyson Perrins Laboratory University of OxfordSouth Parks RoadOxfordOX13QYUK

Six novel benzoylphenylurea chitin inhibitor derivatives were synthesized in the yields of 30%_50% from the readily available starting material chlorothalonil 1 viasequential fluorine exchange, aminolysis, hydrolysis ... 详细信息

关键词： Benzoylphenylureas Chitin inhibitor Chlorothalonil

Activated vascular endothelia regulate invasion of glioma cells through expression of fibronectin

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Chinese Medical Journal 2010年第13期123卷 1754-1761页

作者： liN Zhi-xiong yang li-juan HUANG Qiang FU Jin Department of Neurosurgery the First Affiliated Hospital Fujian Medical University Fuzhou Fujian 350005 China Department of Pharmacology Fujian Medical University Fuzhou Fujian 350004 China Department of Neurosurgery the Second Hospital Suzhou University Suzhou Jiangsu 215004 China Department of Pharmacology the School of Medicine Xiamen University Xiamen 361005 China Department of Pharmacology University of California Irvine USA

Background Previous researches have indicated that glioma invasion may occur within a tumor-host microecology, and that fibronectin may be involved in glioma invasion as an important component of the extracellular matrix. However, how the interaction between tumor cells and vascular endothelial cells affects glioma invasion is poorly understood. The aim of this study was to investigate the effects of the interaction between tumor cells and vascular endothelial cells on glioma invasion, and the relationship of this interaction to fibronectin. Methods The localization of fibronectin in different brain astrocytoma tissues was determined by immunohistochemistry Then, vascular endothelial cells and glioma cells were co-cultured in a Transwell co-culturing system. Fibronectin expression was detected by reverse transcriptase-polymerase chain reaction, immunocytochemistry, and enzyme-linked immunosorbent assay. Additionally, the influence of the interaction between tumor cells and vascular endothelial cells on glioma cell invasion was determined by an in vitro rapid invasion test. Results In brain astrocytoma tissues, fibronectin was present on the endothelial cells, in the extracellular matrix. Fibronectin expression was greater in higher grade tumors than in lower grade tumors. The interaction of glioma cells and vascular endothelial cells in vitro induced fibronectin release from vascular endothelial cells, which in turn stimulated glioma cell migration. This effect was inhibited by fibronectin blocking antibody. Conclusion Glioma cells may induce vascular epithelial cells to express fibronectin, and in turn fibronectin could promote glioma cell invasion.

关键词： glioma fibronectin neoplasm invasiveness tumor-host microecology cell-to-cell communication

Sequence-activated chemiluminescence probes: programmable illumination for high-contrast in vivo sensing and imaging

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Science China Chemistry 2025年第1期68卷 26-34页

作者： Yutao Zhang juan li Wei-Hong Zhu Zhiqian Guo Shanghai Key Laboratory of Functional Materials Chemistry Joint International Research Laboratory of Precision Chemistry and Molecular Engineering Institute of Fine Chemicals School of Chemistry and Molecular Engineering East China University of Science and Technology State Key Laboratory of Bioreactor Engineering Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell MetabolismEast China University of Science & Technology

Sequence-activated chemiluminescence(CL) technique offers a unique approach to detecting specific biomolecules or cellular events with high sensitivity and spatial resolution. The fundamental principle behind sequence-activated CL probes involves tailoring highly-controllable CL signals in response to specific sequences or events of interest, thus enabling high-contrast signals under complex physiological conditions. This sequential activation could be triggered by enzymes, reactive oxygen species(ROS), biothiols or changes in the microenvironment. These unique sequence-activated CL probes bestow several advantages, including minimal background, deep-tissue penetration, and the ability of non-invasive or minimally invasive detection. The sequence-activated CL techniques represent a significant advancement in molecular imaging and diagnostics. In this mini-review, we focus on the latest developments in sequential CL probes, addressing the technical challenges and discussing the future trajectory by harnessing CL signaling and molecular recognition.

关键词： chemiluminescnece sequence-activation in-vivo bioimaging cyclic peroxide

Effects of heat shock protein-antigen peptide complexes (HACs) in melanoma B16 cell line on transplanted tumor development in mice

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白求恩医科大学学报 2001年第5期27卷 457-460页

作者： GONG Shou liang,yang Ying,li Xiu juan,FU Shi bo,SUN Zu yue,CHEN Sha li,li Xiu yi (MH Radiobiology Research Unit,Jilin University,Changchun 130021 China)

目的 :建立黑色素瘤 B16细胞热休克蛋白 -抗原肽复合物 (HACs)的制备方法并测其抑瘤效应。方法 :应用 Sephacryl S- 2 0 0凝胶过滤制备 HAC粗提物 ,应用 SDS- PAGE纯化 HACs,并测其抑瘤效应。结果 :应用 SDS- PAGE纯化的 HAC6 0、 HAC75... 详细信息

目的 :建立黑色素瘤 B16细胞热休克蛋白 -抗原肽复合物 (HACs)的制备方法并测其抑瘤效应。方法 :应用 Sephacryl S- 2 0 0凝胶过滤制备 HAC粗提物 ,应用 SDS- PAGE纯化 HACs,并测其抑瘤效应。结果 :应用 SDS- PAGE纯化的 HAC6 0、 HAC75和 HAC97不同程度地降低肿瘤发生率 ,延迟肿瘤发生时间和减慢肿瘤生长速度。结论 :6 0 0 0 0～ 970 0 0

关键词：黑色素瘤 B16细胞热休克蛋白-抗原肽复合物 HACs 抑瘤效应

Long non-coding RNA small nucleolar RNA host gene 6 aggravates pancreatic cancer through upregulation of far upstream element binding protein 1 by sponging microRNA-26a-5p

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Chinese Medical Journal 2020年第10期133卷 1211-1220页

作者： Xing-Xing Zhang Hua Chen Hui-Ying li Rui Chen Lei He juan-li yang lin-lin Xiao Jin-lian Chen departm ent of Gastroenterology Shanghai University of Medicine&Health Sciences Affiliated Sixth People's Hospital South CampusShanghai 201499China Department of Laboratory Medicine Shanghai University of Medicine&Health Sciences Affiliated Sixth People's Hospital South CampusShanghai 201499China.

Background:Pancreatic cancer(PC)is a highly deadly malignancy with few effective *** aimed to unmask the role that long non-coding RNA small nucleolar RNA host gene 6(SNHG6)plays in PC cells by targeting far upstream element binding protein 1(FUBP1)via microRNA-26a-5p(miR-26a-5p).Methods::SNHG6 expression was predicted by bioinformatics,followed by verification via reverse transcription quantitative polymerase chain ***,the interactions among SNHG6,miR-26a-5p,and FUBP1 were detected through online software analysis,dual luciferase reporter assay and RNA *** that,cells were treated with different small interfering RNAs and/or mimic to determine the interactions among SNHG6,miR-26a-5p,and FUBP1 and their roles in PC ***,the role of SNHG6 in tumor growth in vivo was evaluated by measuring the growth and weight of transplanted tumors in nude mice.A t-test,one-way and two-way analysis of variance were used for data ***::Compared with that in normal tissues,SNHG6 was highly expressed in PC tissues(1.00±0.05 vs.1.56±0.06,t=16.03,Plial cells(HPDE6-C7),SNHG6 showed the highest expression in PANC-1 cells(1.00±0.06 vs.3.87±0.13,t=34.72,Pliferation,migration,and ***6 overexpression exerted the opposite ***6 upregulated FUBP1 expression by sponging *** SNHG6 blocked the growth of PC in ***::S

关键词： Prostatic neoplasms Long non-coding RNA SNHG6 microRNA-26a FUBP1 Proliferation Invasion Migration Apoptosis