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Size-selective hybridization chain reaction for accurate signal amplification in living cancer cells

Science China Chemistry 2024年第12期67卷 4259-4266页

作者： Ting Chen Xiaojiao Wang Rongrong Gao meihe Yuan mei Chen Xiao-Bing Zhang Guoliang Ke State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringCollege of Materials Science and EngineeringHunan UniversityChangsha 410082China

Accurate signal amplification in living cells is highly important in biomedical research and medical *** from its enzyme-free,efficient isothermal signal amplification ability,hybridization chain reaction(HCR)plays an important role in intracellular signal amplification;however,HCR fails the accurate signal amplification in the situation when the properties of some biological targets and analogues are too ***,their signal amplification accuracy for mature mi RNAs is unsatisfactory due to the signal interference of precursor micro RNAs(abbreviated as pre-mi RNAs),which also contain the sequence of mature mi ***,we develop the first example of size-selective hybridization chain reaction probe for accurate signal amplification,which achieved accurate and sensitive biosensing of mature mi RNAs in living cancer *** probe,termed as q Tcage,consists of a DNA nanocage for size-selective responsive to mature mi RNAs,as well as a quadrivalent tetrahedral DNA structure for HCR signal *** from the size-selectivity of DNA nanocage,shorter mature mi RNAs(19–23 nt)rather than longer pre-mi RNAs(60–70 nt)could enter the cavity to release triggers strand,which activates HCR reaction for fluorescence signal *** probe efficiently reduces signal interference of pre-mi RNAs and improves the imaging sensitivity for intracellular mature mi RNAs,which was successfully applied for mature mi RNAs imaging during drug ***,this strategy provides the hybridization chain reaction with the feature of size-selective ability,which holds promise for further accurate signal amplification in biological processes study and clinical diagnostics.

关键词： hybridization chain reaction microRNA DNA nanotechnology

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The gender difference of utilization of cardiac implantable electronic device in China： data from Arrhythmia Interventional Therapy Data Registry

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Journal of Geriatric Cardiology 2018年第4期15卷 310-314页

作者： Ruo-Han CHEN Ke-Ping CHEN Wei HUA Jing XU Lin CHEN yang-Gang SU Xi SU Jian-Gang ZOU Ji YAN Jing-Feng WANG Bao-Peng TANG mei-Xiang XIANG Shu ZHANG Center of Arrhythmia Fuwai Hospital Chinese Academy of Medical Sciences Peking union Medical College Beijing China Department of Cardiology Tian Jing Chest Hospital Tianjing China Department of Cardiology FuJian Provincial Hospital Fuzhou China Department of Cardiology ZhongShan Hospital FuDang University Shanghai China Department of Cardiology WuHan Asia Cardiology Hospital Wuhan China Department of Cardiology the Ftrst Affiliated Hospital of Nangjing Medical University NanJing China Department of Cardiology AnHui Provincial Hospital Hefei China Department of Cardiology Sun- Yat-Sen Memorial Hospital Guangzhou China Department of Cardiology the First Affiliated Hospital of XinJiang Medical University Urumichi China Department of Cardiology the Second Affiliated Hospital of ZheJiang University of School of Medical Hangzhou China

Background Cardiac implantable electronic devices （CIEDs） greatly improve survival and life quality of patients. However, there are gender differences regarding both the utilization and benefit of these devices. In this prospective CIED registry, we aim to appraise the gender differences in CIED utilization in China. Methods Twenty centers from 14 provinces in China were included in our registry study. All patients who underwent a CIED implantation in these twenty centers between Jan 2015 and Dec 2016 were included. Results A total of 8570 patients were enrolled in the baseline cohort, including 7203 pacemaker, 664 implantable cardiac defibrillators （ICD） implants and 703 cardiac resynchronization therapy device （CRT/D）. Totally, 4117 （48.0%） CIED patients were female, and more than 59% pacemaker patients were female, but women account only one third of ICD or CRT/D implantation in this registry. There were significant differences between genders at pacemaker and ICD indications. Female was more likely received a pacemaker due to sick sinus syndrome （SSS）（63.9% vs. 51.0%, P 〈 0.001）. Female patients receiving an ICD were more likely due to cardiac ion channel disease （29.2% vs. 4.2%, P 〈 0.001）. The percentage of utilization of dual-chamber pacemaker in female patients was significantly higher than male （85.3% vs. 81.1%, P 〈 0.001）. But male patients were more likely received a cardiac resynchronization therapy devices with defibrillator than female （56.5% vs. 41.9%, P = 0.001）. In pacemaker patient, male was more likely to have structure heart disease （31.3% vs. 28.0%, P = 0.002）. In ICD patient, male patients were more likely to have ischemic heart disease （48.2% vs. 29.2%, P 〈 0.001）. The mean age of women at the time of CRT/D implantation was older than men （P = 0.014）. Nonischemic cardiomyopathy （70.9%） was the most common etiology in the patients who underwent the treat？ment of CRT/D, no matter male or female. Conclusions In real-world setting, female do have

关键词： Cardiac implantable electronic devices Gender Registry

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减税能否影响企业出口?——基于增值税有效税率的考察

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商业研究 2020年第6期 96-106页

作者：杨慧梅南开大学经济学院天津300071

本文以减税为切入点,选取2000-2006年中国工业企业和海关进出口贸易数据,利用2004年东北地区增值税转型改革的政策冲击构建工具变量,从出口决策与出口绩效两个维度系统考察增值税有效税率对企业出口的影响,并识别其内在作用机制。研究发... 详细信息

本文以减税为切入点,选取2000-2006年中国工业企业和海关进出口贸易数据,利用2004年东北地区增值税转型改革的政策冲击构建工具变量,从出口决策与出口绩效两个维度系统考察增值税有效税率对企业出口的影响,并识别其内在作用机制。研究发现,增值税有效税率降低可以提高企业的出口倾向,改善企业的出口绩效。这一效果因企业所有制、规模、盈利水平及行业技术水平不同而有所差异,表现为非国有企业、小型企业、低盈利水平企业及一般技术行业受到的影响更大。对影响机制的检验表明,生产率提升与产品竞争力提高是增值税有效税率影响企业出口决策与出口绩效的主要渠道。因此,应继续深化税制改革,减轻企业税负,注重公平化、合理化,发挥减税政策的引导和激励作用。

关键词：减税出口决策出口绩效增值税有效税率

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泄漏声振动传播信道辨识及其在泄漏点定位中的应用

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振动工程学报 2007年第3期20卷 260-267页

作者：杨进文玉梅李平重庆大学光电技术及系统教育部重点实验室

在基于声振动信号处理的传统泄漏定位方法中,已知振动信号在被测管道中的传播速度是该类方法的应用前提,但在实际检测中发现,该声速很难准确获知。采用盲系统辨识方法估计泄漏信号传播信道响应函数,并从中提取源信号从漏点传播到采集点... 详细信息

在基于声振动信号处理的传统泄漏定位方法中,已知振动信号在被测管道中的传播速度是该类方法的应用前提,但在实际检测中发现,该声速很难准确获知。采用盲系统辨识方法估计泄漏信号传播信道响应函数,并从中提取源信号从漏点传播到采集点的时间信息,从而不依赖泄漏信号传播速度定位漏点。盲辨识中,采用O verlap-Save和相关函数配准原理构建代价函数,解决高阶信道估计和信道间的病态问题;采用遗传算法对多目标函数进行全局最优化,避免梯度算法收敛陷入局部最小点。应用表明,通过泄漏声振动传播信道辨识,在声速未知情况下,泄漏定位平均误差在1 m以内。

关键词：泄漏定位泄漏声振动盲系统辨识遗传算法

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The Fano-Like Resonance in Self-Assembled Trimer Clusters

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Chinese Physics letters 2013年第7期30卷 236-239页

作者： ZHANG mei LI Liang-Sheng ZHENG Ning SHI Qing-Fan Faculty of Computers Guangdong University of TechnologyGuangzhou 510006 Science and Technology on Electromagnetic Scattering Laboratory Beijing 100854 School of Physics Beijing Institute of TechnologyBeijing 100081

We illustrate that three identical nanocylinders self-assembled into a trimer with a small interparticle gap separation support strong Fano-like *** asymmetry line shape of the Fano-like resonance can be controlled by modifying the cluster structures,and a narrow linewidth between a peak and a dip is displayed in the line *** resonant behaviors of the trimers could be strengthened by increasing the permittivity of the nanorods,and the asymmetry resonance is highly sensitive to the angle of the incident wave due to the orientation-dependent coupling between the cylinders in the *** present study provides a way to investigate the environmental change caused by the reshaped asymmetry line,which can be applied to future high-performance resonance sensors.

关键词： cylinders resonant narrow

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Genotoxicity evaluation of drinking water sources in human peripheral blood lymphocytes using the comet assay

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Journal of Environmental Sciences 2008年第4期20卷 487-491页

作者： WU Yulin CHEN Haigang LI Zhaoli SUN Liwei QU Mengmeng LI mei KONG Zhiming State Key Laboratory of Pollution Control and Resource Reuse School of the Environment Nanjing University Nanjing 210093 China Jiangsu Institute of Planned Parenthood Research Nanjing 210036 China

The potential harm of organic pollutants in drinking water to human health is widely focused on in the wodd; more and more pollutants with genotoxic substances are released into the aquatic environment. Water source samples were collected from 7 different localities of Nanjing City. The potential genotoxicity of organic extracts from drinking water sources were investigated by means of the comet assay in human peripheral lymphocytes. The results showed that all the organic extracts from all the water source samples could induce DNA damages of human peripheral blood lymphocytes at different levels. A significant difference （P 〈 0.01） was observed when compared with the solvent control, The DNA damage increased with the increase of the dosage of the original water source. Significant differences of DNA damage were observed in different drinking water sources, as shown by the multiple comparisons analysis at the dosage of 100x; the degree of DNA damage treated by Hushu waterworks （at town level） was the most serious, the arbitrary units （AU） was 141.62±6.96, however, that of Shangyuanmen waterworks （at city level） was only 109.64±2.97. The analysis also revealed that the genotoxicity of town＇s water sources was higher than that of the city. The results demonstrated that the comet assay can be successfully applied to the genotoxicity monitoring programs of drinking water sources.

关键词： comet assay drinking water sources genotoxicity human peripheral blood lymphocyte

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抗肿瘤药物的不良反应及防治措施

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中国新药杂志 2008年第21期17卷 1889-1893页

作者：杨梅汤致强中国医学科学院肿瘤医院药剂科北京100021

抗肿瘤药物的不良反应较多,文中从血液系统、消化系统、循环系统、泌尿系统、神经系统、生殖系统、皮肤反应和过敏反应等方面对肿瘤化疗药物的不良反应及防治措施进行了归纳、分析,为临床医生更加合理用药、临床药师进行药学监护提供参考。

关键词：抗肿瘤药物不良反应防治

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Excited State Intramolecular Proton Transfer of 1-Hydroxyanthraquinone

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Chinese Journal of Chemical Physics 2015年第5期28卷 -页

作者： Si-mei Sun Song Zhang Kai Liu Ya-ping Wang Miao-miao Zhou Bing Zhang State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics Wuhan Institute of Physics and Mathematics Chinese Academy of Sciences Wuhan 430071 China

The excited state intra-molecular proton transferdynamics of 1-hydroxyanthraquinone in solution are investigated by femtosecond transient absorption spectroscopy and quantum chemistry calculations. Two characteristic bands of excited state absorption and stimulated emission are observed in transient absorption spectra with the excitation by the pump wavelength of 400 nm. From the delayed stimulated emission signal, the time scale of the intra-molecular proton transfer is determined to be about 32 fs. The quantum chemistry calculations show that the molecular orbits and the order of the S2 and S1 states are reversal and a conical intersection is demonstrated to exist along the proton transfer coordinate. After proton transfer, the second excited state of tautomer populated via the conical intersection undergoes the internal conversion with ～200 fs and the following intermolecular energy relaxation with ～16 ps. The longer component 300 ps can be explained in terms of the relaxation from excited-state tautomer to its ground state. From our observations, two proton transfer pathways via a conical intersection are proposed and the dominated one preserves the molecular orbits.

关键词： Proton transfer Conical intersection Transient absorption spectroscopy 1-Hydroxyanthraquinone

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Dehalogenation of Aryl Halides Catalyzed by MontK10 Immobilized PVP-Pd-Sn Catalyst in Aqueous System

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Chinese Chemical letters 2001年第4期12卷 287-290页

作者： Xiao Li ZHEN Jian Rong HAN Ru Hong KANG Xing mei OUyang Department of Essential Education Hebei Science(Technique UniversityShijiazhuang 050018 Department of Chemistry Heibei Normal UniversityShijiazhuang 050016

A series of PVP-Pd-Sn/MontK10 catalysts were prepared by immobilization of PVP[poly(N-vinyl-2-pyrrolidone)] supported bimetallic catalyst using MontK10 as carrier. This catalyst has good catalytic activity for hydrogen transfer dehalogenation of aryl halides. The catalytic reaction was carried out in aqueous system in the presence of phase transfer catalyst and sodium formate as hydrogen source. The catalyst with loading Pd 0.19wt% and molar ratio of Pd/Dn 8:1 gives the highest activity and good stability. This catalyst is more reducible with NaBH4. It is also found that the catalyst is easily separated from the reaction system.

关键词： Phase transfer catalyst aryl halide dehalogenation MontK10

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Complete stabilization of severely As-contaminated soil by a simple H2O2 pre-oxidation method combined with non-toxic TMT-15 and FeCl3·6H2O

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International Journal of Minerals,Metallurgy and Materials 2019年第9期26卷 1105-1112页

作者： Chang-sheng Yue Ben Peng Wei Tian Guang-hua Lu Gui-bo Qiu mei Zhang Central Research Institute of Building and Construction Co. Ltd.Metallurgical Corporation of China Group (MCC Group)Beijing 100088China National Engineering Research Center for Industrial Environmental Protection Beijing 100088China School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing 100083China

The stabilization of severely As-polluted soil has been a challenge, especially for the extremely toxic As(Ⅲ) contaminants. In this study, soil with a high As concentration(26084 mg/kg) was availably stabilized by a H2O2 pre-oxidation assisted TMT-15(Na3S3C3N3 solution with a mass fraction of 15%) and FeCl3·6 H2O stabilization method. The results showed that the combination of the two stabilizers(i.e., TMT-15 and FeCl3·6 H2O) presented a better stabilization behavior than either stabilizer used individually. The use of the H2O2 pre-oxidation assisted TMT-15 and FeCl3·6 H2O stabilization approach not only converted the As(Ⅲ) to As(Ⅴ) but also reduced the toxic leaching concentration of As to 1.61 mg/L, which is a safe level, when the additions of TMT-15 and FeCl3·6 H2O were 2 mL and 0.20 g, respectively. Thus, using only a simple H2O2 pre-oxidation to combine clean stabilization with non-toxic stabilizers TMT-15 and FeCl3·6 H2O could render the severely As-contaminated soil safe for disposal in a landfill.

关键词： severely As-contaminated soil non-toxic stabilizers combining stabilization pre-oxidation

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