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reduction of Chilling Injury and Ultrastructural Damage in Cherry Tomato Fruits After Hot Water Treatment

Agricultural sciences in china 2009年第3期8卷 304-310页

作者： yang jing, fu mao-run, zhao yu-ying and mao lin-chun department of food science and nutrition, college of biosystem engineering and food science, zhejiang university, hangzhou 310029, p.r.china [a]department of food science and nutrition College of Biosystem Engineering and Food Science Zhejiang University Hangzhou 310029 P.R. China

The effects of hot water treatment in alleviating chilling injury and reducing ultrastructural damage of mature-green cherry tomatoes （Lycopersicun esculentum cv. cerasiform Alef） were investigated. Mature-green cherry tomato fruits were treated in water at 40℃ or 45℃ for 5 min or 15 min, and then stored at 5℃ for 19 days followed by ripening at 20℃. Water treatment at 40℃ for 15 min increased tolerance of cherry tomato fruits to chilling stress, indicating as low outbreak of skin lesion, high color a＊ value, and low electrolyte leakage. Treated fruits showed typical climacteric respiration and developed normal red color with chlorophyll degradation and lycopene accumulation during ripening, while fruits without treatment failed to develop red color and suffered skin lesion. After 19 days of chilling, heated fruits showed the conversion of chloroplast to chromoplast with the disappearance of thylakoids. Mitochondria and other cell organelles were not adversely affected in treated fruits. However, ultrastructures in pericarp cells in control fruits severely damaged with extensive disorganization of cytoplasm, swelled chloroplasts, distorted and unstacked thylakoids. Chloroplast was the first and most severely impacted organelle by chilling stress. Hot water treatment （40℃ for 15 min） before storage alleviated chilling injury in cherry tomato fruits. The results suggest that chilling injury is related with the damage of cell structure under chilling stress.

关键词： cherry tomato chilling injury heat treatment ultrastructure

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Effects of Drying processes on the Antioxidant properties in Sweet potatoes

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Agricultural sciences in china 2010年第10期9卷 1522-1529页

作者： yang jing CHEN Jin-feng zhao yu-ying mao lin-chun department of food science and nutrition College of Biosystem Engineering and Food Science Zhejiang University Hangzhou 310029 P.R. China

The effects of different drying methods （hot-air drying, microwave drying and vacuum-freeze drying） on the antioxidant activity and antioxidants in sweet potato （lpomoea batatas L. Lain.） tubers were investigated to determine the potential drying process. Sweet potato tubers were cut into 5 mm thick slices, steamed at 100℃ for 10 rain, then dried in either hotair, microwave, or vacuum-freeze. The dried sweet potatoes in microwave possessed the highest antioxidant activity, while the lowest activity was observed in hot-air dried samples. The phenolic contents were positively correlated with scavenging activity and reducing power of DppH^＋. The microwave drying retains the highest antioxidant activity with the highest content of phenolic compounds in dried sweet potatoes. β-carotene and ascorbic acid showed minor contribution to the antioxidant activity in dried sweet potatoes.

关键词： sweet potato (lpomoea batatas L. Lam.) drying antioxidant activity antioxidants

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responses of phospholipase D and lipoxygenase to mechanical wounding in postharvest cucumber fruits

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Journal of zhejiang university-science B(Biomedicine & Biotechnology) 2010年第6期11卷 443-450页

作者： yu-ying zhao chun-lu QIAN Ji-cheng CHEN Yan pENG lin-chun mao department of food science and nutrition College of Biosystem Engineering and Food Seience Zhejiang University Hangzhou 310029 China college of Horticulture and Gardening Yangtze University Jingzhou 434025 China

This study was to investigate the responses of phospholipase D（pLD） and lipoxygenase（LOX） to mechanical wounding in postharvest cucumber（Cucumis sativus ***-2） ***-associated Ca2＋ content,activities and gene expression of pLD and LOX,and contents of phosphatidylcholine（pC）,phosphatidylinositol（pI）,and phosphatidic acid（pA） were determined in cucumber fruits following mechanical *** show that pLD and LOX activities increased with the pLD and LOX mrNAs which are upregulated upon wounding,while membrane-associated Ca^2＋ content *** with the increase of pLD and LOX activities,accumulation of pA and losses of pC and pI were observed in all fruits,but there were differences of degrees between wounded and control *** suggest that pLD and LOX might be the main hydrolytic enzymes of phospholipids in postharvest cucumber fruits participating in the mechanical wounding *** activation of pLD and LOX might be the result of gene expression,which could be This study was to investigate the responses of phospholipase D（pLD） and lipoxygenase（LOX） to mechanical wounding in postharvest cucumber（Cucumis sativus ***-2） ***-associated Ca^2＋ content,activities and gene expression of pLD and LOX,and contents of phosphatidylcholine（pC）,phosphatidylinositol（pI）,and phosphatidic acid（pA） were determined in cucumber fruits following mechanical *** show that pLD and LOX activities increased with the pLD and LOX mrNAs which are upregulated upon wounding,while membrane-associated Ca2＋ content *** with the increase of pLD and LOX activities,accumulation of pA and losses of pC and pI were observed in all fruits,but there were differences of degrees between wounded and control *** suggest that pLD and LOX might be the main hydrolytic enzymes of phospholipids in postharvest cucumber fruits participating in the mechanical wounding *** activation of pLD and LOX might be

关键词： Cucumber Mechanical wounding phospholipase D Lipoxygenase phospholipid

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Biomarkers of aging

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science china(Life sciences) 2023年第5期66卷 893-1066页

作者： Aging Biomarker Consortium Hainan Bao Jiani Cao Mengting Chen Min Chen Wei Chen Xiao Chen Yanhao Chen yu Chen yutian Chen Zhiyang Chen Jagadish K Chhetri yingjie Ding Junlin Feng Jun Guo Mengmeng Guo Chuting He yujuan Jia Haiping Jiang ying jing Dingfeng Li Jiaming Li jingyi Li Qinhao Liang rui Liang Feng Liu Xiaoqian Liu Zuojun Liu Oscar Junhong Luo Jianwei Lv jingyi Ma Kehang mao Jiawei Nie Xinhua Qiao Xinpei Sun Xiaoqiang Tang Jianfang Wang Qiaoran Wang Siyuan Wang Xuan Wang Yaning Wang yuhan Wang rimo Wu Kai Xia fu-Hui Xiao lingyan Xu yingying Xu Haoteng Yan Liang yang ruici yang yuanxin yang Yilin ying Le Zhang Weiwei Zhang Wenwan Zhang Xing Zhang Zhuo Zhang Min Zhou rui Zhou Qingchen Zhu Zhengmao Zhu Feng Cao Zhongwei Cao piu Chan Chang Chen Guobing Chen Hou-Zao Chen Jun Chen Weimin Ci Bi-Sen Ding Qiurong Ding Feng Gao jing-Dong JHan Kai Huang Zhenyu Ju Qing-peng Kong Ji Li Jian Li Xin Li Baohua Liu Feng Liu lin Liu Qiang Liu Qiang Liu Xingguo Liu Yong Liu Xianghang Luo Shuai Ma Xinran Ma Zhiyong mao jing Nie Yaojin peng jing Qu Jie ren ruibao ren Moshi Song Zhou Songyang Yi Eve Sun yu Sun Mei Tian Shusen Wang Si Wang Xia Wang Xiaoning Wang Yan-Jiang Wang yunfang Wang Catherine CL Wong Andy peng Xiang Yichuan Xiao Zhengwei Xie Daichao Xu jing Ye rui yue Cuntai Zhang Hongbo Zhang Liang Zhang Weiqi Zhang Yong Zhang yun-Wu Zhang Zhuohua Zhang Tongbiao zhao yuzheng zhao Dahai Zhu Weiguo Zou Gang pei Guang-Hui Liu Aging Biomarker Consortium(ABC) Beijing 100101China CAS Key Laboratory of Genomic and precision Medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijing 100101China State Key Laboratory of Stem Cell and reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China department of Dermatology Xiangya HospitalCentral South UniversityChangsha 410008China Hunan Key Laboratory of Aging Biology Xiangya HospitalCentral South UniversityChangsha 410008China National Clinical research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangsha 410008China Clinic Center of Human Gene research Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Hubei Clinical research Center of Metabolic and Cardiovascular Disease Huazhong University of Science and TechnologyWuhan 430022China Hubei Key Laboratory of Metabolic Abnormalities and Vascular Aging Huazhong University of Science and TechnologyWuhan 430022China Stem Cell Translational research Center Tongji HospitalTongji University School of MedicineShanghai 200065China department of Nuclear Medicine Daping HospitalThird Military Medical UniversityChongqing 400042China CAS Key Laboratory of nutrition Metabolism and Food SafetyShanghai Institute of Nutrition and HealthUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200031China Shanghai Key Laboratory of Maternal Fetal Medicine Clinical and Translational Research Center of Shanghai First Maternity and Infant HospitalFrontier Science Center for Stem Cell ResearchShanghai Key Laboratory of Signaling and Disease ResearchSchool of Life Sciences and TechnologyTongji UniversityShanghai 200092China The department of Endovascular Surgery The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China Key Laboratory of regenerative Medicine of Ministry of Education Institute of Ageing and Regenerative MedicineJ

Aging biomarkers are a combination of biological parameters to(i)assess age-related changes,(ii)track the physiological aging process,and(iii)predict the transition into a pathological *** a broad spectrum of aging biomarkers has been developed,their potential uses and limitations remain poorly *** immediate goal of biomarkers is to help us answer the following three fundamental questions in aging research:How old are we?Why do we get old?And how can we age slower?This review aims to address this ***,we summarize our current knowledge of biomarkers developed for cellular,organ,and organismal levels of aging,comprising six pillars:physiological characteristics,medical imaging,histological features,cellular alterations,molecular changes,and secretory *** fulfill all these requisites,we propose that aging biomarkers should qualify for being specific,systemic,and clinically relevant.

关键词： aging senescence biomarker clock

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盐地碱蓬发酵动力学模型构建及抗氧化功能评价

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食品科学 2023年第6期44卷 172-179页

作者：徐晶赵学群王珍珍殷树鹏沈晓艳沙如意毛建卫山东科技职业学院生物与化学工程系山东潍坊261053 北京中医药养生工程研究院北京102200 浙江科技学院生物与化学工程学院浙江杭州310023 潍坊高新生物园发展有限公司山东潍坊261205

对盐地碱蓬发酵过程中不同发酵时间的酵母菌、乙醇、还原糖、pH值、可滴定酸、总酚等代谢产物及羟自由基清除率、超氧阴离子自由基清除率、还原力等抗氧化指标进行跟踪检测,研究其发酵动力学和抗氧化能力。采用SGompertz、Doseresp、Log... 详细信息

对盐地碱蓬发酵过程中不同发酵时间的酵母菌、乙醇、还原糖、pH值、可滴定酸、总酚等代谢产物及羟自由基清除率、超氧阴离子自由基清除率、还原力等抗氧化指标进行跟踪检测,研究其发酵动力学和抗氧化能力。采用SGompertz、Doseresp、Logistic和Boltzmann模型建立盐地碱蓬发酵动力学模型;利用相关性与主成分分析法构建盐地碱蓬发酵的综合评价指标体系。结果表明,用Doseresp和Boltzmann方程建立酵母菌生长动力学模型,用SGompertz方程建立乙醇产量生成动力学模型,用Logistic方程建立还原糖基质消耗动力学模型,拟合系数r2分别为0.977、0.995、0.982,均呈良好拟合效果;相关性分析结果表明,总酚和有机酸是盐地碱蓬发酵液中抗氧化能力主要来源;主成分分析表明,综合评价指标呈先升高趋于稳定再下降的趋势,综合考虑发酵终点为第8天。该研究揭示了盐地碱蓬发酵过程中代谢产物以及抗氧化活性的变化规律,为盐地碱蓬发酵液的精准制备提供理论基础。

关键词：盐地碱蓬发酵代谢产物动力学模型抗氧化功能综合评价指标

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