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Genomic and transcriptomic analyses of Citrus sinensis varieties provide insights into Valencia orange fruit mastication trait formation

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Horticulture Research 2021年第1期8卷 2861-2871页

作者： guizhi Feng Xiu Ai Hualin Yi Wenwu Guo Juxun Wu Key Laboratory of Horticultural Plant Biology Ministry of EducationHuazhong Agricultural UniversityWuhanPR China

Valencia orange(Citrus sinensis Osbeck)(VO)is a type of late-ripening sweet orange whose ripening occurs 4 to 5 months later than that of the mid-ripening common sweet orange(CO).Notably,the mastication trait of VO fruit is inferior to that of CO *** date,how inferior pulp mastication trait forms in VO has not been *** this study,13 VO varieties and 12 CO varieties were subjected to whole-genome resequencing.A total of 2.98 million SNPs were identified from 25 varieties,and a SNP molecular marker was developed to distinguish VO and ***,144 and 141 genes identified by selective sweep analysis were selected during VO and CO evolution,*** on gene functional enrichment analysis,most of the selected VO genes were related to the stress response and lignin ***,we comparatively analyzed the transcriptome profiles of peel and pulp tissues among three VO varieties and three CO varieties,and the results demonstrated differences in lignin biosynthesis between VO and CO ***,coexpression network analysis was performed to identify hub genes of lignin-related and variety-specific networks,which included CsERF74,CsNAC25,CsHSFB3,CsSPL4/13,***,this study provides important insights into the mastication trait formation of Valencia orange fruit.

关键词： orange insight varieties

Spectroscopic Investigation of the Interaction between Human Serum Albumins and 10-Hydroxycamptothecin

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Wuhan University Journal of Natural Sciences 2009年第3期14卷 257-261页

作者： LI guizhi LIU Yongming College of Chemistry Yantai University Yantai 264005Shandong China

The binding reaction between 10-hydroxycamptothecin （10-HCPT） and human serum albumins （HSA） is studied by means of fluorescence spectroscopy, UV-Vis absorption spectrum, IH NMR spectrum, and molecular simulation. The results indicate that the binding reaction of 10-HCPT and HSA is a single static quenching process, and the binding equilibrium constant for 10-HCPT binding with HSA is estimated K0-4.93×10^4 L · mol-I at 25 ℃ with the molar ratio of I ： 1. The distance （r） and energy transfer efficiency（E） between donor （HSA） and acceptor （10-HCPT） are obtained as follows, r=3.51 nm; E-0.27. The enthalpy change （△Hφ） and entropy change （△Sφ） are calcu- lated at different temperatures, and the hydrophobic force and shidipole force are the functions in the reaction. The results show that 10-HCPT binds within the subdomain II A of HSA by the hydrophobic force, and the 10-OH and 20-OH of 10-HCPT bind with both residue Leu-238 of HSA and Ala 291 of HSA by hydrogen bonds.

关键词： 10-hydroxycamptothecin human serum albumins (HSA) fluorescence spectroscopy UV absorption spectroscopy 1H NMR spectrum molecular simulation

Characterization and mapping of QTLs on chromosome 2D for grain size and yield traits using a mutant line induced by EMS in wheat

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The Crop Journal 2015年第2期3卷 135-144页

作者： guizhi Zhang Yingying Wang Ying Guo Yan Zhao Fanmei Kong Sishen Li State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology Shandong Agricultural University

Production of mutants with altered phenotypes is a powerful approach for determining the biological functions of genes in an organism. In this study, a high-grain-weight mutant line M8008 was identified from a library of mutants of the common wheat cultivar YN15 treated with ethylmethane sulfonate(EMS). F2 and F2:3generations produced from crosses of M8008 × YN15(MY) and M8008 × SJZ54(MS) were used for genetic analysis. There were significant differences between M8008 and YN15 in plant height(PH), spike length(SL),fertile spikelet number per spike(FSS), grain width(GW), grain length(GL), GL/GW ratio(GLW), and thousand-grain weight(TGW). Most simple correlation coefficients were significant for the investigated traits, suggesting that the correlative mutations occurred in M8008. Approximately 21% of simple sequence repeat(SSR) markers showed polymorphisms between M8008 and YN15, indicating that EMS can induce a large number of mutated loci. Twelve quantitative trait loci(QTLs) forming QTL clusters(one in MY and two in MS) were detected. The QTL clusters coinciding with(MY population) or near(MS population) the marker wmc41 were associated mainly with grain-size traits, among which the M8008 locus led to decreases in GW, factor form density(FFD), and TGW and to increases in GLW. The cluster in the wmc25–barc168 interval in the MS population was associated with yield traits, for which the M8008 locus led to decreased PH, spike number per plant(SN), and SL.

关键词： Common wheat Mutant Simple sequence repeat(SSR) Quantitative trait locus(QTL) Grain size trait Yield trait

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混凝土实体重力坝断面拓扑与形貌优化研究

水力发电 2025年第1期51卷 51-56页

作者：李景丽王桂智张宏伟郑州西亚斯学院建筑工程学院河南郑州451100 江苏省水利勘测设计研究院有限公司江苏扬州225127 河海大学土木与交通学院江苏南京210024 河海大学水利水电学院江苏南京210024

为了获得较为经济的混凝土实体重力坝断面形式,提出了基于拓扑与形貌优化方法的混凝土实体重力坝优化设计方法。该方法以混凝土实体重力坝断面柔度最小为优化目标,对混凝土实体重力坝断面先进行拓扑优化设计,然后进行形貌优化设计,进而... 详细信息

为了获得较为经济的混凝土实体重力坝断面形式,提出了基于拓扑与形貌优化方法的混凝土实体重力坝优化设计方法。该方法以混凝土实体重力坝断面柔度最小为优化目标,对混凝土实体重力坝断面先进行拓扑优化设计,然后进行形貌优化设计,进而有效地减小混凝土实体重力坝断面面积。某混凝土实体重力坝断面优化结果表明:优化得到的断面与一般传统方法设计的断面较为接近,但断面面积有所减少,且抗滑稳定安全系数与应力等均满足相关规范要求,证明了优化设计方法的合理性;重力坝形貌优化断面优于拓扑优化断面与传统设计断面;随着形貌优化迭代次数的增加,抗滑稳定安全系数逐渐增大,坝体竖向应力与竖向位移最大值逐渐减少;重力坝形貌优化断面在坝体上部存在颈缩现象,该现象可为重力坝设计提供新的思考方向。

关键词：混凝土重力坝断面设计拓扑优化形貌优化变密度法

PREPARATION AND PROPERTIES OF THE MICRO-POWDER PHASE AND A NEW PHASE OF AMMONIUM TETRAMOLYBDATES

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中国有色金属学会会刊：英文版 1997年第1期7卷 19-22页

作者： Yin, Zhoulan Chen, Qiyuan Zhang, Hengzhong Zhou, guizhi Li, Yuangao Zhang, Pingmin Central South Univ of Technology Changsha China

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关键词： new phase of ammonium tetramolybdate micro powder phase of ammonium tetramolybdate phase transformation molybdenum

Electro-Pulse-Boring(EPB): Novel Super-Deep Drilling Technology for Low Cost Electricity

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Journal of Earth Science 2015年第1期26卷 37-46页

作者： Hans O.Schiegg Arild Rdland guizhi Zhu David A.Yuen SwissG eo Power AG Stadtturmstrasse 19 CH-5401Switzerland Department of Petroleum Engineering and Applied Geophysics Norwegian University of Science and Technology7491 TrondheimNorway Department of Earth Sciences ETH-Zurich CH-8092Switzerland Department of Earth Sciences and Minnesota Supercomputing Institute University of MinnesotaMinneapolis MN 55455USA School of Environmental Studies China University of Geosciences

The inexhaustible heat deposit in great depths （5-10 km） is a scientific fact. Such deposit occurs around the globe. Thereby, everybody is enabled to generate autonomously clean and renewable energy, ample electricity and heat. The economical exploration and exploitation of this superdeep geothermal heat deposit requires a novel drilling technique, because the currently only deep drilling method （Rotary） is limited to about 5 km, due to the rising costs, depending exponentially on depth. Electro-pulse-boring （EPB） is a valuable option to Rotary drilling. EPB, originally investigated in Russia, is ready to be developed for industrialization. The feasibility of EPB is proven by many boreholes drilled up to 200 m in granite （crystalline）. Estimates show outstanding low costs for drilling by EPB： 100 E/m for a borehole with a large diameter （φ） such as 20 （50 cm）, independent on depth and applicable likewise for sediments and crystalline rocks, such as granite. The current rate of penetration （ROP） of 3 m per hour is planned to be augmented up to 35 m per hour, and again, irrespective whether in sedimentary or crystalline formations. Consequently, a 10 km deep borehole with φ 50 cm will ultimately be drilled within 12 days. EPB will create new markets, such as：（i） EPB shallow drilling for geotechnics, energy piles, measures in order to mitigate natural hazards, etc., （ii） EPB deep drilling （3-5 km） for hydro-geothermics, exploration campaigns etc. and （iii） EPB super-deep drilling （5-10 km） for petro-geothermies, enabling the economic generation of electricity. The autonomous and unlimited supply with cost efficient electricity, besides ample heat, ensures reliably clean and renew- able energy, thus, high supply security. Such development will provide a substantial relief to cope with the global challenge to limit the climate change below 2 ℃. The diminution of fossil fuels, due to the energy transition in order to mitigate the climate change, implies likewise the decr

关键词： drilling technology electro pulse petro-geothermics geoenergy geothermal electricity climate change air pollution.

Carbonate dynamics in a tropical coastal system in the South China Sea featuring upwelling, river plumes and submarine groundwater discharge

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Science China Earth Sciences 2022年第12期65卷 2267-2284页

作者： Wei yang Xianghui GUO Zhimian CAO Jianzhong SU Liguo GUO Lifang WANG Yi XU Tao HUANG Yan LI Yanping XU Zhe WANG guizhi WANG Minhan DAI State Key Laboratory of Marine Environmental Science College of Ocean and Earth SciencesXiamen UniversityXiamen 361102China

This study examined carbonate dynamics in the northwestern South China Sea(NWSCS),an area jointly influenced by upwelling,river plumes and submarine groundwater *** were obtained from two cruises conducted in summer 2009 and *** 2009,a high salinity-low temperature water mass occurred nearshore off northeastern Hainan Island,indicative of upwelling,commonly referred to as HNEU.A river plume fueled primarily by local rivers and characterized by low salinity and high temperature was observed in the NWSCS off the mainland roughly along the 30 m *** 2012,coastal upwelling off northeastern Hainan Island was not detectable at the surface,but was observed at a different location off eastern Hainan Island(HEU).River plume waters in 2012 were patchily distributed,with a low salinity zone further westerly than that in 2009 and another on the mid-shelf of the NWSCS sourced from the Pearl River which reached out~250 km from the mouth of the Pearl River *** 2009,elevated dissolved inorganic carbon(DIC)and total alkalinity(TA)occurred in the coastal plume,where submarine groundwater discharge contributed DIC and TA additions of 38.9±20.5 and 42.5±22.3μmol kg^(−1),respectively,with a DIC/TA ratio of~0.92,which made a minor contribution to the variation of seawater partial pressure of CO_(2)(pCO_(2)),pH and the aragonite saturation state index(Ω_(arag)).Additionally,high surface phytoplankton production consumed DIC of 10.0±10.4μmol kg^(−1) but did not significantly affect TA,which dominated pCO_(2) drawdown in the coastal plume water and increased the pH andΩ_(arag) at *** groundwater discharge was also observed in the region influenced by upwelling,but to a lesser degree than that impacted by coastal *** pH andΩ_(arag) and higher pCO_(2) values than in offshore waters were observed downstream of the upwelling system,attributable largely to organic matter remineralization with a DIC addition of 23.8±8.4μmol kg^(−1).In 2012,submari

关键词： Carbonate system Northwestern South China Sea Coastal upwelling Submarine groundwater discharge Ocean acidification

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Interictal Electrophysiological Source Imaging Based on Realistic Epilepsy Head Model in Presurgical Evaluation:A Prospective Study

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Chinese Journal of Electrical Engineering 2023年第1期9卷 61-70页

作者： Ruowei Qu Zhaonan Wang Shifeng Wang Le Wang Alan Wang guizhi Xu State Key Laboratory of Reliability and Intelligence of Electrical Equipment Hebei University of TechnologyTianjin 300401China Tianjin Universal Medical Imaging Diagnostic Center Tianjin 300110China Department of Functional Neurosurgery Huanhu HospitalTianjin 300350China Auckland Bioengineering Institute The University of AucklandGraftonAuckland 1010New Zealand

Invasive techniques are becoming increasingly important in the presurgical evaluation of *** the electrophysiological source imaging(ESI)of interictal scalp electroencephalography(EEG)to localize the epileptogenic zone remains a *** accuracy of the preoperative localization of the epileptogenic zone is key to curing *** T1 MRI and the boundary element method were used to build the realistic head *** solve the inverse problem,the distributed inverse solution and equivalent current dipole(ECD)methods were employed to locate the epileptogenic ***,a combination of inverse solution algorithms and Granger causality connectivity measures was *** ECD method exhibited excellent focalization in lateralization and localization,achieving a coincidence rate of 99.02%(p<0.05)with the stereo *** combination of ECD and the directed transfer function led to excellent matching between the information flow obtained from intracranial and scalp EEG *** ECD inverse solution method showed the highest performance and could extract the discharge information at the cortex level from noninvasive low-density EEG ***,the accurate preoperative localization of the epileptogenic zone could reduce the number of intracranial electrode implantations required.

关键词： Epilepsy epileptogenic zone realistic head model functional brain connectivity ESI

CVT-Based Asynchronous BCI for Brain-Controlled Robot Navigation

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Cyborg and Bionic Systems 2023年第1期4卷 318-329页

作者： Mengfan Li Ran Wei Ziqi Zhang Pengfei Zhang guizhi Xu Wenzhe Liao State Key Laboratory of Reliability and Intelligence of Electrical Equipment School of Health Sciences and Biomedical EngineeringHebei University of Technology300132 TianjinChina Hebei Key Laboratory of Bioelectromagnetics and Neuroengineering 300132 TianjinChina Tianjin Key Laboratory of Bioelectromagnetic Technology and Intelligent Health 300132 TianjinChina School of Artificial Intelligence Hebei University of Technology300132 TianjinChina

Brain-computer interface(BCI)is a typical direction of integration of human intelligence and robot *** control is an essential form of combining human and robot agents in a common task,but still faces a lack of freedom for the human *** paper proposes a Centroidal Voronoi Tessellation(CVT)-based road segmentation approach for brain-controlled robot navigation by means of asynchronous *** electromyogram-based asynchronous mechanism is introduced into the BCI system for self-paced control.A novel CVT-based road segmentation method is provided to generate optional navigation goals in the road area for arbitrary goal *** event-related potential of the BCI is designed for target selection to communicate with the *** robot has an autonomous navigation function to reach the human selected goals.A comparison experiment in the single-step control pattern is executed to verify the effectiveness of the CVT-based asynchronous(CVT-A)BCI *** subjects participated in the experiment,and they were instructed to control the robot to navigate toward a destination with obstacle avoidance *** results show that the CVT-A BCI system can shorten the task duration,decrease the command times,and optimize navigation path,compared with the single-step ***,this shared control mechanism of the CVT-A BCI system contributes to the promotion of human and robot agent integration control in unstructured environments.

关键词： robot agent Robot