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Melatonin prevents endothelial cell pyroptosis via regulation of long noncoding RNA MEG3/miR-223/NLRP3 axis

中国药理学与毒理学杂志 2019年第10期 759-760页

作者： LIU Xin ZHANG Yong yang bao-feng Harbin Medical University

OBJECTIVE Atherosclerosis(AS) is an inflammatory disease linked to endothelial *** is reported to possess substantial anti-inflammatory properties, which has proven to be effective in AS. Emerging literature suggests that pyroptosis plays a critical role during AS progression. However, whether pyroptosis contributes to endothelial dysfunction and the underlying molecular mechanisms remained *** study was designed to investigate the antipyroptotic effects of melatonin in atherosclerotic endothelium and to elucidate the potential mechanisms. METHODS ApoE-/-mice were fed a high-fat diet to establish an atherosclerotic model, then divided into normal diet(ND) group, normal diet+melatonin(ND+melatonin) group, high fat diet(HFD)group and high fat diet+melatonin(HFD+melatonin) *** 12 weeks, HE and oil Red O staining were used to detect the formation of atherosclerosis; qRT-RCR and Western blotting were used to detect the expression of NLRP3, ASC, IL-1β, IL-18, GSDMD, NF-κB, miR-223 and MEG3 in aortic endothelium; The luciferase assay was used to detect the binding of mi R-223 to MEG3. Human aortic endothelial cells(HAECs) were pretreated with ox-LDL. After melatonin treatment, qRT-RCR was used to detect the expression of mi R-223 and MEG3. Western blotting was used to detect NLRP3, ASC, c-caspase1,p-caspase1, GSDMD expression. In addition, after overexpressing MEG3 and knocking out mi R-223, the pyroptosis of HAECs was also detected. RESULTS We found intragastric administration of melatonin for 12 weeks markedly reduced the atherosclerotic plaque in ***, melatonin also attenuated the expression of pyroptosis-related genes, including NLRP3, ASC,cleaved caspase1, NF-κB/GSDMD, GSDMD N-termini,IL-1β, and IL-18 in aortic endothelium of melatonin-treated animals. Consistent antipyroptotic effects were also observed in ox-LDL-treated human aortic endothelial cells(HAECs). We found that lnc RNA MEG3 enhanced pyroptosis in HAECs. M

关键词： melatonin atherosclerosis miR-223 MEG3 NLRP3

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Long noncoding RNAs: emerging players in cardiac electrical and structural remodeling

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中国药理学与毒理学杂志 2019年第10期 805-806页

作者： ZHANG Yong ZHANG Ying SUN Li-hua yang bao-feng Department of Pharmacology(State-Province Key Laboratories of Biomedicine-Pharmaceutics of China Key Laboratory of Cardiovascular Medicine ResearchMinistry of Education) College of Pharmacy Harbin Medical University Faculty of Medicine Department of Pharmacology and Herapeutics Melbourne School of Biomedical Sciences Dentistry and Health Sciences University of Melbourne

OBJECTIVE Cardiac remodeling results in poor prognosis because of its association with ventricular dysfunction and malignant arrhythmias. METHODS We firstly performed the microarray profiling and bioinformatics analyses of long non-coding RNAs(lnc RNAs) in a mouse model of heart failure(HF) characterized by cardiac remodeling. Then we measured the levels of selected lnc RNAs with high profiles linking to cardiac dysfunction in clinical samples. RESULTS We found that circulating level of ZFAS1 was markedly lower in AMI than in non-AMI subjects. While CCRR is decreased in patients with *** we identified ZFAS1 as an inhibitor of SERCA2 a by binding to SERCA2 a protein. Abnormally increased ZFAS1 in MI impaired cardiac function. Most prominently,we identified a conserved functional domain of ZFAS1,which is much shorter and responsible for its deleterious effects. Moreover, we found CCRR silencing induced arrhythmias by destruction of intercalated discs and gap junctions to slow longitudinal cardiac conduction in healthy mice. CCRR overexpression improves cardiac conduction by blocking endocytic trafficking of connexin43(Cx43) to prevent its degradation via binding to Cx43-interacting protein CIP85. We identified the functional domain of CCRR, which can reproduce the functional roles of full-length CCRR. CONCLUSION Our studies suggest ZFAS1 and CCRR play pivotal roles in cardiac remodeling, and provide therapeutic strategy for ameliorating cardiac dysfunction.

关键词： lncRNA ZFAS1 CCRR cardiac remodeling cardiac function

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Impact of Arsenic Trioxide on LS-174T Cell Growth in vitro and the Activity of Telomerase

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Chemical Research in Chinese Universities 2006年第2期22卷 209-212页

作者： WANG Xi-shan WANG Gui-yu XU Hai-tao DONG De-li FU Song-bin ZHANG Qi-fan yang bao-feng Department of Abdominal Surgery the Affiated Tumor Hospital Department of Pharmacology Department of Genetics Harbin Medical University Harbin 150001 P. R. China

The therapeutic action of arsenic trioxide（ As203 ） on solid tumors has aroused widespread interest among scholars. To study the impact of As2O3 on human colorectal carcinoma cells（ LS-174T cell） and the activity of telomerase, the methods of PCR-ELISA, flow cytometry （FCM） and MTT assay in vitro were utilized. The results show that （1） with an increase in the concentration of As2O3, the ratio of living the cells to dead cells decreases significantly, and the IC50 value is 5.23 μg/mL; （2） the cells of the experimental groups can endure a series of morphological changes similar to the features of apoptosis ; （3） the apoptotic curves of FCM pictures appear after 24 h, and the cells show the apoptosis in a time-dependent manner; （4） As2O3 can inhibit the activity of telomerase of the cell extraction obviously in a concentration-dependent and time-dependent manner after 24 h. It can be concluded from the experiment results in vitro that As2O3 can induce the apoptosis of LS-174T cells and inhibit the telomerase activity. Therefore, it has been proposed, for the first time, that these two factors （the apoptosis of LS-174T cells and the inhibition to the telomerase activity） are important causes of the LS-174T cell death caused by As2O3.

关键词： Arsenic trioxide Telomerase Colorectal carcinoma Apoptosis

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Priming　effect　of　substance　Ponsuper　oxideanionradical　production　inhumanneu　trophils

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中国药理学与毒理学杂志 1996年第2期 14-18页

作者： bao Li-Hua, yang bao-feng, feng Bin 1 , LI bao-Xin, HE Shu-Zhuang, LI Wen-Han (Department of Pharmacology, Harbin Medical University, Harbin 150086 1 Department of Medicine, First Hospital of Harbin, Harbin 150010)

神经肽Ｐ物质能激活多种参与炎症和免疫反应的细胞．然而Ｐ物质直接刺激人粒细胞产生超氧阴离子（Ｏ÷２）需要较高的浓度（＞１０μｍｏｌ·Ｌ－１）．本实验观察了低浓度Ｐ物质对人粒细胞超氧阴离子生成的活化作用．结果表明，Ｐ物质... 详细信息

神经肽Ｐ物质能激活多种参与炎症和免疫反应的细胞．然而Ｐ物质直接刺激人粒细胞产生超氧阴离子（Ｏ÷２）需要较高的浓度（＞１０μｍｏｌ·Ｌ－１）．本实验观察了低浓度Ｐ物质对人粒细胞超氧阴离子生成的活化作用．结果表明，Ｐ物质在低浓度时（３μｍｏｌ·Ｌ－１）能活化人粒细胞，使甲酰基－甲硫氨酰基－亮氨酰基苯丙氨酸（ｆＭＬＰ）刺激产生的超氧阴离子明显增多．Ｐ物质的这一活化作用呈剂量和时间依赖性．在无细胞外钙的情况下，Ｐ物质没有这种活化作用．Ｐ物质活化ｆＭＬＰ引起的Ｏ÷２生成增多作用可被神经激肽（ＮＫ）－１受体阻断剂ｓｐｅｎｔｉｄｅ（１μｍｏｌ·Ｌ－１），磷脂酶Ｃ抑制剂Ｕ－７３１２２（１０ｎｍｏｌ·Ｌ－１）以及Ｃａ２＋通道阻断剂尼卡地平（１μｍｏｌ·Ｌ－１）所抑制．这些发现提示，Ｐ物质活化人粒细胞可能是经ＮＫ－１受体偶联的磷脂酰肌醇代谢途径，并且是细胞外钙依赖性的

关键词： P物质中性粒细胞,人超氧化物钙,细胞外

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城轨轨道系统排水设计的几个常见问题

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铁道工程学报 2016年第12期33卷 105-108页

作者：杨宝峰铁道第三勘察设计院集团有限公司天津300251

研究目的:排水设计是轨道系统设计的主要内容之一。由于工程环境条件的复杂性,特别是地下结构空间的局限性,线路排水设计存在较多困难,导致实际的排水效果不是很理想。废水若不能及时排除,会对隧道结构、轨道结构以及电气设备的安全性... 详细信息

研究目的:排水设计是轨道系统设计的主要内容之一。由于工程环境条件的复杂性,特别是地下结构空间的局限性,线路排水设计存在较多困难,导致实际的排水效果不是很理想。废水若不能及时排除,会对隧道结构、轨道结构以及电气设备的安全性产生影响,进而影响线路正常运营。本文旨在研究如何在保证道床结构安全的前提下,合理利用有限的地下结构空间和线路纵坡,将隧道渗漏水、冲洗和消防废水排放至废水泵站,再集中排入市政废水排放通道。研究结论:(1)总结多个项目设计的经验、教训、体会,研究参考国外试验成果和国内高速铁路应用实例,提出了平坡车站、道岔转辙机坑、道床内设置废水泵站以及敞口段的轨道系统排水设计方法及处理措施;(2)本研究成果可为城市轨道交通轨道系统的排水设计提供参考。

关键词：排水设计平坡车站转辙机坑废水泵站敞口段

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Effects of arsenic trioxide on voltage-dependent potassium channels and on cell proliferation of human multiple myeloma cells

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Chinese Medical Journal 2007年第14期120卷 1266-1269页

作者： ZHOU Jin WANG Wei WEI Qing-fang feng Tie-ming TAN Li-jun yang bao-feng Department of Hematology First Clinical Medical College of Harbin Medical University Harbin 150001 China Pharmacological Department of Harbin Medical University Harbin 150001 China

Arsenic trioxide （ATO） can induce cellular apoptosis ,and inhibit the activities of multiple myeloma （MM）cells in vitro, but how it works is not very clear. Recent studies showed that ATO worked on the voltagedependent potassium channel and L-type calcium channel in myocardial cells, but the effect of ATO on ion channels of tumor cells was rarely reported. As the potassium channel plays an important role in controlling cell proliferation, we studied the effects of ATO on the voltage-dependent potassium current （Ikv） of the voltage-dependent potassium channel in an MM cell line, and probed into the relationship between changes of the Ikv caused by ATO and cell proliferation.

关键词： multiple myeloma arsenicals ion channel cell cycle proliferation

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Effects of 3-aminobenzamide on expressions of poly（ADP ribose） polymerase and apoptosis inducing factor in cardiomyocytes of rats with acute myocardial infarction

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Chinese Medical Journal 2009年第11期122卷 1322-1327页

作者： ZHAO Yu-juan WANG Jian-hua FU Bing MA Mu-xin LI bao-xin HUANG Qi yang bao-feng Department of Cardiology First Clinical Medical College Harbin Medical University Harbin 150001 China Pharmacological College Harbin Medical University Harbin 150086 China Centre for Cellular Morphology Harbin Medical University Harbin 150086 China

Background Poly（ADP-ribose） polymerase （PARP） plays an important role in cell survival and death. However, the mechanisms involved are not fully understood. Therefore, we investigated the effect of inhibition of PARP on acute myocardial infarction （AMI） at different time points in rats. Methods AMI was induced in rats by ligating the left anterior descending coronary artery. One group received 3-aminobenzamide （3-AB, a kind of PARP inhibitor）（30 mg/kg） by intraperitoneal injection. The changes of ultramicrostructure of cardiocytes in infarction region were noted, PARP cleavage was measured by Western blotting, and expressions of protein of PARP and apoptosis inducing factor （AIF） were measured by immunohistochemical staining after treatment with 3-AB for 2 hours, 4 hours, 6 hours, 1 week, 4 weeks and 8 weeks. Results Few damages to the ultramicrostructure of cardiocytes were observed after treatment with 3-AB. PARP cleavage was detected as early as 4 hours and markedly increased by 6 hours following AMI without 3-AB, but was not found until 6 hours following AMI treated with 3-AB. There were significant differences between 3-AB and AMI groups at the same time points. The expression of PARP was observed gradually increased, but that of AIF was suppressed for 6 hours after treatment of 3-AB, compared with AMI groups in positive cells at the same time points. There was significantly less cleavage of PARP and more PARP expression in 3-AB treated group compared with AMI and control groups at all matched time points. Conclusions Our results suggest that 3-AB inhibits degradation of PARP, increases the expression of PARP protein, and suppresses the expression of AIF protein. Inhibition of PARP activity may protect cardiocytes in rats with AMI and reduce apoptosis.

关键词： 3-aminobenzamide acute myocardial infarction poly(ADP-ribose) polymerase apoptosis inducing factor DNA repair apoptosis

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证据冲突的衡量方法研究

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华北工学院学报 2005年第1期26卷 58-60页

作者：杨风暴中北大学电子信息工程系山西太原030051

　对于有冲突的证据,D-S证据理论无法使用或得出错误的结果,解决该问题的基础首先是研究证据冲突的衡量方法.分析了D-S证据理论在合成冲突证据时遇到的问题,定义了一些衡量证据间冲突程度的参数,包括证据一致度、证据冲突度、证据冲突... 详细信息

　对于有冲突的证据,D-S证据理论无法使用或得出错误的结果,解决该问题的基础首先是研究证据冲突的衡量方法.分析了D-S证据理论在合成冲突证据时遇到的问题,定义了一些衡量证据间冲突程度的参数,包括证据一致度、证据冲突度、证据冲突强度、证据冲突/一致度等.通过实例证实了这些参数能够较好地说明证据间存在冲突的程度.

关键词：衡量方法冲突程度问题定义基础实例错误参数例证

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Kanglexin, a novel anthraquinone compound,protects against myocardial ischemic injury in mice by suppressing NLRP3 and pyroptosis

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中国药理学与毒理学杂志 2019年第10期 770-771页

作者： BIAN Yu LI Xin PANG Ping WANG Ning WANG Jin-hui XIAO Wei DU Wei-jie yang bao-feng Department of Pharmacology Harbin Medical University Department of Medicinal Chemistry and Natural Medicine Chemistry Harbin Medical University Jiangsu Kanion Pharmaceutical Co. Ltd.

OBJECTIVE To investigate the myocardial protection effect of a novel anthraquinone compound（4,5-dihydroxy-7-methyl-9,10-anthraquinone-2-ethyl succinate） named Kanglexin（KLX） in vivo and in vitro. METHODS Male C57 BL/6 mice were pre-treated either with KLX(20, 40 mg·kg;·d;, ig) or vehicle for 7 consecutive days prior to ligation of coronary artery to induce permanent MI. Neonatal mouse cardiomyocytes（NMCMs） were exposed to hypoxia or LPS to detect the myocardial protection effect of KLX. Echocardiographic and myocardial infarct size were used to evaluate the effect of KLX on MI treated mice. Electron microscopic examination and H&E staining were preformed to determine morphological changes of each group. Western blotting and q PCR were used to detect the expressions of pyroptosis relative proteins. The numbers of TUNEL and PI-positive cells were measured to assess pyroptotic cell *** KLX reduced myocardial infarct size and LDH release and improved cardiac function as compared to vehicle-treated mice 24 h after MI. We found that MI triggered NLRP3 inflammasome activation leading to conversion of IL-1β and IL-18 into their active mature forms in the heart. We also showed that MI induced pyroptosis, as evidenced by increased DNA fragmentation, mitochondrial swelling and cell membrane rupture as well as increased levels of several pyroptosis-related proteins, including GSDMD, N-GSDMD and cleaved caspase-1. All these detrimental alterations were prevented by KLX administration. In hypoxia-or LPS-treated NMCMs,we showed that 10 μmol·L;KLX decreased the elevated levels of TUNEL-and PI-positive cel s as wel as pyroptosisrelated proteins. CONCLUSION KLX prevents MI-induced cardiac damages and cardiac dysfunction at least partly through attenuating NLRP3 and subsequent cardiomyocyte pyroptosis, and it is worthy of more rigorous investigations for its potential for alleviating ischemic heart disease.

关键词： myocardial infarction anthraquinone Kanglexin ischemic injury NLRP3 inflammasome pyroptosis

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基于支持度变换和top-hat分解的双色中波红外图像融合

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光谱学与光谱分析 2014年第4期34卷 1144-1150页

作者：蔺素珍杨风暴陈磊中北大学仪器科学与动态测试教育部重点实验室山西太原030051

为了解决用多尺度top-hat分解法融合双色中波红外图像时经常存在对比度提升有限、边缘区域失真较重的问题,提出了基于支持度变换和top-hat分解相结合的融合方法。先用支持度变换法将双色中波图像分解为低频图像和支持度图像序列;再从最... 详细信息

为了解决用多尺度top-hat分解法融合双色中波红外图像时经常存在对比度提升有限、边缘区域失真较重的问题,提出了基于支持度变换和top-hat分解相结合的融合方法。先用支持度变换法将双色中波图像分解为低频图像和支持度图像序列;再从最后一层低频图像中用多尺度top-hat分解法提取各自的亮信息和暗信息;用灰度值取大法分别融合亮信息和暗信息;通过灰度值归一化和高斯滤波分别增强亮、暗信息融合图像;然后融合两低频图像和亮、暗信息增强图像;将融合图像作为新的低频图像和用灰度值取大法融合得到的支持度融合图像序列进行支持度逆变换,得到最终融合图像。该方法的实验结果同采用单一的支持度变换法融合和多尺度top-hat分解法融合相比,融合图像的对比度提升了11.69%,失真度降低了63.42%,局部粗糙度提高了38.12%。说明提出的从低频图像提取亮暗信息,并经过分别融合、增强,再与低频图像进行融合,能有效破解红外融合图像对比度提升和边缘区域失真度降低之间的矛盾,为提高图像融合质量提供了新方法。

关键词：双色中波图像融合支持度变换 top-hat分解 Dual-color mid-wave infrared (MWIR) Support value transform (SVT)

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