检索结果-南通市图书馆

科技视界 2016年第20期 230-231,236页

作者： yang jie-hai XIAO Yi-ting Fujian Ningde Nuclear Power Co. Ltd.

1 Introduction Since the commissioning of the turbine driven auxiliary feedwater pump,within three years operation,a series of failures have occurred,such as failure of over-speed trip gear,low output,high vibration,trip gear reset failure,and so *** to the manufacturer’s advice and on-site maintenance experience,we carried out modification on

关键词： valve maintenance advice Turbine manufacturer Nuclear steam Auxiliary turbine reset

miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

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Neural Regeneration Research 2025年第1期20卷 277-290页

作者： Meng Cong Jing-Jing Hu Yan Yu Xiao-Li Li Xiao-Ting Sun Li-Ting Wang Xia Wu Ling-jie Zhu Xiao-Jia yang Qian-Ru He Fei Ding hai-Yan Shi School of Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Coinnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Physiology Jiangsu Health Vocational CollegeNanjingJiangsu ProvinceChina Department of Pathology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve ***-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular ***,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain *** investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose *** mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest *** performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest ***,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded ***,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously *** findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in ***-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell *** helps to explain the mechanism of action of the secretome and e

关键词： axotomy cell-free therapy conditioned medium extracellular vesicles hypoxic preconditioning microRNA oxygen-glucose deprivation peripheral nerve injury Schwann cell precursors

Maintaining moderate levels of hypochlorous acid promotes neural stem cell proliferation and differentiation in the recovery phase of stroke

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Neural Regeneration Research 2025年第3期20卷 845-857页

作者： Lin-Yan Huang Yi-De Zhang jie Chen hai-Di Fan Wan Wang Bin Wang Ju-Yun Ma Peng-Peng Li hai-Wei Pu Xin-Yian Guo Jian-Gang Shen Su-Hua Qi School of Medical Technology Xuzhou Key Laboratory of Laboratory DiagnosticsXuzhou Medical UniversityXuzhouJiangsu ProvinceChina School of Pharmacy Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina Department of Laboratory Medicine Branch Hospital of Huai’an First People’s HospitalHuai’anJiangsu ProvinceChina Department of Laboratory Medicine the Affiliated Hospital of Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina School of Chinese Medicine The University of Hong KongHong Kong Special Administrative RegionChina

It has been shown clinically that continuous removal of ischemia/reperfusion-induced reactive oxygen species is not conducive to the recovery of late ***,previous studies have shown that excessive increases in hypochlorous acid after stroke can cause severe damage to brain *** previous studies have found that a small amount of hypochlorous acid still exists in the later stage of stroke,but its specific role and mechanism are currently *** simulate stroke in vivo,a middle cerebral artery occlusion rat model was established,with an oxygen-glucose deprivation/reoxygenation model established in vitro to mimic *** found that in the early stage(within 24 hours)of ischemic stroke,neutrophils produced a large amount of hypochlorous acid,while in the recovery phase(10 days after stroke),microglia were activated and produced a small amount of hypochlorous ***,in acute stroke in rats,hypochlorous acid production was prevented using a hypochlorous acid scavenger,taurine,or myeloperoxidase inhibitor,4-aminobenzoic acid *** results showed that high levels of hypochlorous acid(200μM)induced neuronal apoptosis after oxygen/glucose deprivation/***,in the recovery phase of the middle cerebral artery occlusion model,a moderate level of hypochlorous acid promoted the proliferation and differentiation of neural stem cells into neurons and *** suggests that hypochlorous acid plays different roles at different phases of cerebral ischemia/reperfusion *** levels of hypochlorous acid(5 and 100μM)promoted nuclear translocation ofβ-*** transfection of single-site mutation plasmids,we found that hypochlorous acid induced chlorination of theβ-catenin tyrosine 30 residue,which promoted nuclear ***,our study indicates that maintaining low levels of hypochlorous acid plays a key role in the recovery of neurological function.

关键词： cell differentiation cerebral ischemia/reperfusion injury chlorination hypochlorous acid microglia neural stem cell neurogenesis nuclear translocation stroke β-catenin

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论广西北部湾经济区档案人力资源开发一体化

广西中医学院学报 2011年第1期14卷 118-120页

作者：杨海洁广西中医学院广西南宁530001

档案人力资源是档案部门更好地开发档案信息、提供更优质服务的行为主体和活力源泉,广西北部湾经济区的档案部门必须通过有效的人力资源开发获取竞争优势。笔者认为,广西北部湾经济区是一个跨区域的经济体,档案人力资源开发一体化能适... 详细信息

档案人力资源是档案部门更好地开发档案信息、提供更优质服务的行为主体和活力源泉,广西北部湾经济区的档案部门必须通过有效的人力资源开发获取竞争优势。笔者认为,广西北部湾经济区是一个跨区域的经济体,档案人力资源开发一体化能适应经济区的发展需要,

关键词：广西北部湾经济区档案人力资源开发一体化

CNKSR2 interactome analysis indicates its association with the centrosome/microtubule system

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Neural Regeneration Research 2025年第8期20卷 2420-2432页

作者： Lin Yin Yalan Xu jie Mu Yu Leng Lei Ma Yu Zheng Ruizhi Li Yin Wang Peifeng Li hai Zhu Dong Wang Jing Li Institute for Translational Medicine The Affiliated Hospital of Qingdao UniversityMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina School of Basic Medicine Qingdao UniversityQingdaoShandong ProvinceChina College of Life Sciences and School of PharmacyMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina Department of Urology Qingdao Municipal Hospital Affiliated to Qingdao UniversityQingdaoShandong ProvinceChina

The protein connector enhancer of kinase suppressor of Ras 2(CNKSR2),present in both the postsynaptic density and cytoplasm of neurons,is a scaffolding protein with several protein-binding *** of the CNKSR2 gene have been implicated in neurodevelopmental disorders,particularly intellectual disability,although the precise mechanism involved has not yet been fully *** has demonstrated that CNKSR2 plays a role in facilitating the localization of postsynaptic density protein complexes to the membrane,thereby influencing synaptic signaling and the morphogenesis of dendritic ***,the function of CNKSR2 in the cytoplasm remains to be *** this study,we used immunoprecipitation and high-resolution liquid chromatography-mass spectrometry to identify the interactors of *** a combination of bioinformatic analysis and cytological experiments,we found that the CNKSR2 interactors were significantly enriched in the proteome of the *** also showed that CNKSR2 interacted with the microtubule protein DYNC1H1 and with the centrosome marker *** colocalization analysis confirmed the centrosomal localization of *** we downregulated CNKSR2 expression in mouse neuroblastoma cells(Neuro 2A),we observed significant changes in the expression of numerous centrosomal *** manipulation also affected centrosome-related functions,including cell size and shape,cell proliferation,and ***,we found that CNKSR2 interactors were highly enriched in de novo variants associated with intellectual disability and autism spectrum *** findings establish a connection between CNKSR2 and the centrosome,and offer new insights into the underlying mechanisms of neurodevelopmental disorders.

关键词： autism spectrum disorder centrosome CNKSR2 intellectual disability interactome mass spectrometry microtubule neurodevelopmental disease protein complexes protein-protein interactions

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嗜热菌分离筛选及分子分类初探

厦门大学学报（自然科学版） 2006年第2期45卷 276-279页

作者：阮灵伟刘欣杨海杰李钫厦门大学生命科学学院福建厦门361005 国家海洋局第三海洋研究所海洋生物遗传资源重点实验室福建厦门361005 厦门大学海洋与环境学院福建厦门361005

从厦门周边地区温泉采集得到样品,在75℃和80℃的温度下进行培养并分离得到60株嗜热菌.为了从大量的菌株中筛选出不同的菌株,有效地简化筛选过程,应用了细菌全蛋白SDS-PAGE电泳、RAPD和16S-23S rDNA间隔区比较分析等分子生物学方法从中... 详细信息

从厦门周边地区温泉采集得到样品,在75℃和80℃的温度下进行培养并分离得到60株嗜热菌.为了从大量的菌株中筛选出不同的菌株,有效地简化筛选过程,应用了细菌全蛋白SDS-PAGE电泳、RAPD和16S-23S rDNA间隔区比较分析等分子生物学方法从中筛选得到7株种属不同的嗜热菌,并进一步运用16S rDNA序列分析鉴定其种属关系,为深入研究奠定了基础.

关键词：嗜热菌全蛋白 RAPD 16S-23S rDNA间隔区

奥氮平对骨髓间充质干细胞成脂分化的作用及机制

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解剖学报 2016年第3期47卷 341-347页

作者：纪晨燕李永海李萌杨海杰冯志伟新乡医学院生命科学技术学院河南新乡453003 河南省干细胞与生物治疗工程研究中心河南新乡453003

目的探讨第2代抗精神病药物(SGA)奥氮平(olanzapine)对小鼠骨髓间充质干细胞(BMSCs)体外脂肪分化的影响及其作用机制。方法培养小鼠BMSCs,MTT法检测不同浓度奥氮平对BMSCs增殖的影响;通过油红O染色观察细胞形态,Western blotting检测脂... 详细信息

目的探讨第2代抗精神病药物(SGA)奥氮平(olanzapine)对小鼠骨髓间充质干细胞(BMSCs)体外脂肪分化的影响及其作用机制。方法培养小鼠BMSCs,MTT法检测不同浓度奥氮平对BMSCs增殖的影响;通过油红O染色观察细胞形态,Western blotting检测脂肪分化标志基因蛋白αP2和C/EBPβ的表达,Real-time PCR检测成脂相关基因Leptin、C/EBPα和TNF-α的mRNA表达情况;同时Western blotting检测Akt信号通路上下游相关分子的表达水平及磷脂酰肌醇3激酶(PI3K)/Akt特异性阻断剂对小鼠BMSCs脂肪分化的影响。结果 MTT法结果显示,20μmol/L奥氮平对BMSCs的毒性最小;油红O染色可见加入奥氮平的细胞内脂肪滴明显多于对照组;Western blotting结果显示,αP2和C/EBPβ的表达水平较对照组上升了36%(P<0.01)和25%(P<0.05);Realtime PCR结果显示,Leptin、C/EBPα和TNF-α的表达水平较对照组分别上升68%(P<0.001)、79%(P<0.01)和60%(P<0.01),均具有统计学意义;Western blotting检测结果显示,p-Akt的含量明显高于对照组,磷酸化糖原合成激酶3β(p-GSK-3β)的表达随着p-Akt表达的增加逐渐减少;加入PI3K/Akt特异性阻断剂(LY294002)后αP2和C/EBPβ的表达受到抑制,细胞中的脂肪滴也明显减少。结论奥氮平可能通过促使PI3K/Akt信号通路中Akt的磷酸化水平升高来促进小鼠BMSCs的脂肪分化。

关键词：奥氮平骨髓间充质干细胞脂肪分化 Akt信号通路免疫印迹法小鼠

运用噬菌体展示随机肽库筛选与内毒素结合的高亲和性多肽

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第三军医大学学报 2008年第24期30卷 2262-2266页

作者：杨海捷段光杰刘友生陈锐余德文杨宇第三军医大学西南医院病理学研究所重庆400038

目的从噬菌体展示随机肽库中筛选与内毒素结合的多肽序列,并进行鉴定。方法以内毒素脂多糖(lipopolysaccharide, LPS)为靶分子对噬菌体展示随机十二肽库进行4轮亲和筛选,获得与LPS结合的噬菌体克隆,应用结合实验和克隆斑抑制实验进一步... 详细信息

目的从噬菌体展示随机肽库中筛选与内毒素结合的多肽序列,并进行鉴定。方法以内毒素脂多糖(lipopolysaccharide, LPS)为靶分子对噬菌体展示随机十二肽库进行4轮亲和筛选,获得与LPS结合的噬菌体克隆,应用结合实验和克隆斑抑制实验进一步确证。挑选结合力强的克隆进行DNA测序,推导出呈现的多肽序列,应用生物信息学软件进行多肽序列分析和同源性分析。结果经4轮亲和筛选从噬菌体展示随机十二肽库中筛选获得了86个克隆,挑选12个结合力强的克隆进行DNA序列测序及生物信息学分析,结合本项目组的噬菌体展示随机七肽库筛选结果推导出呈现的多肽序列为HWQWPHWSPPP(命名为P11肽)。检索相关数据库发现此肽序列未被申请专利,体内约908种蛋白与其结构相匹配,其中包含有与LPS相互作用的位点。结论通过对噬菌体展示随机肽库的淘选,获得与LPS结合的高亲和性多肽,为进一步以这些多肽为先导物进行定向进化研究提供了实验依据和结构基础。

关键词：内毒素噬菌体展示随机肽库多肽生物信息学

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内毒素结合肽的改良、原核表达及纯化

中国生物工程杂志 2006年第3期26卷 42-46页

作者：孙亚丽刘友生杨海捷第三军医大学西南医院病理研究所重庆400038

目的:对人内毒素结合肽(endotoxinbindingpeptide,EBP)进行改良,获得突变体mEBP(mutateofendotoxinbindingpeptide,mEBP)基因并进行原核表达,纯化获得高纯度的mEBP。方法:应用PCR定点诱变方法获得mEBP基因,构建PinpointXa3/mEBP融合表达载体,在BL21(DE3)pLysS宿主菌进行表达,溶菌酶裂解法提取包涵体,融合蛋白经PinpointTMXa纯化后,由factorXa酶切,获得目的肽,RPHPLC纯化获得高纯度的mEBP。结果:应用PCR定点诱变技术完成了EBP第5,18位谷氨酰胺→赖氨酸的定点突变,且通过原核表达,色谱纯化获得了高纯度的mEBP。结论:成功获得高纯度的mEBP,为下一步的抗内毒素功能检测奠定基础。

关键词：内毒素结合肽突变表达纯化

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浅谈中草药合理种植与贮藏

现代化农业 2008年第11期 8-9页

作者：张铎杨海杰黑龙江省农垦总局农业局哈尔滨150036 黑龙江省双鸭山农场

黑龙江垦区现有林地面积89.5万hm2,区域森林覆盖率达19.1%,有着生物资源比较优势,其中天然中草药材资源不仅丰富,而且人工种植也有成功经验.黑龙江垦区得天独厚的自然资源为垦区发展中药材生产提供了有利条件.

关键词：中草药合理种植黑龙江垦区贮藏森林覆盖率中药材生产林地面积生物资源