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The 5'-flanking cis-acting elements of the human ε-globin gene associates with the nuclear matrix and binds to the nuclear matrix proteins

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Cell Research 1998年第3期8卷 209-218页

作者： yan zhi jiang RUO LAN QIAN(Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200051, China) Shanghai Institute of Cell Biology Chinese Academy of Sciences Shanghai China

The nucleax mains attachment regions(MARs) and the binding nuclear matrix proteins in the 5'-flalildng cisacting elements of the humanε-globin gene have been examined. Using in vitro DNA-matrix binding assay,it has been shown that the positive stage-specific regulatory element (ε-PREII, -446bp-419bp) upstream of this gene could specifically associate with the nuclear matrix from K562 cells, indicating thatε-PREII mad be an erythroidspecilic facultstive MAR. In gel mobility shift assay and Southwestern blotting assal an eothroid-specific nuclear matrix protein (ε-NMPk) in K562 cells has been revealed to bind to this positive regulatory element (E-PREII). Furthermore, we demonstrated that the silencer (-392hp -177bp) uP8tream of the humanε-globin gene could associate with the nuclear matrices from K562, HEL and Raji cells. In addition, the nucleax matrix proteins prepared from these three cell lines could also bind to this silencer, suggesting that this silencer element linght be a constitutive nuclear mains attachment region (constitutive MAR). Our results demonstrated that the nucleax madrid and nuclear mains proteins lxilght play an important role in the regulation of the human 5-globin gene expression.

关键词： Human ε-globin gene nuclear matrix attachment regions nuclear matrix proteins K562 cells

Liver injury in COVID-19:Detection,pathogenesis,and treatment

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World Journal of Gastroenterology 2021年第22期27卷 3022-3036页

作者： Yue Cai Li-Ping Ye Ya-Qi Song Xin-Li Mao Li Wang yan-zhi jiang Wei-Tao Que Shao-Wei Li Key Laboratory of Minimally Invasive Techniques&Rapid Rehabilitation of Digestive System Tumor of Zhejiang Province Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical UniversityLinhai 317000Zhejiang ProvinceChina Department of Gastroenterology Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical UniversityLinhai 317000Zhejiang ProvinceChina School of Medicine Zhejiang UniversityHangzhou 310000Zhejiang ProvinceChina College of Basic Medicine Inner Mongolia Medical UniversityHohhot 010000Inner Mongolia Autonomous RegionChina Department of Gastroenterology and Hepatology Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200000China Department of Surgery Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200000China

In the early December 2019,a novel coronavirus named severe acute respiratory syndrome coronavirus 2 was first reported in Wuhan,China,followed by an outbreak that spread around the *** studies have shown that liver injury is common in patients with coronavirus disease 2019(COVID-19),and may aggravate the severity of the ***,the exact cause and specific mechanism of COVID-associated liver injury needs to be elucidated *** this review,we present an analysis of the clinical features,potential mechanisms,and treatment strategies for liver injury associated with *** hope that this review would benefit clinicians in devising better strategies for management of such patients.

关键词： COVID-19 Liver injury Clinical features Potential mechanism Treatment

Primary Studies on Burnup Estimation of Irradiated Uranium by Dtermining 236U/238U in Contaminated Environmental Samples jiang Xiao-yan,ZHANG Ji-long,ZOU Wei,Chang zhi-yuan

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Primary Studies on Burnup Estimation of Irradiated Uranium b...

The imbalance of helper T lymphocytes and cytotoxic T lymphocytes in acute renal transplantation rejection

作者： jiang Xiao-yan ZHANG Ji-long ZOU Wei Chang zhi-yuan

U/U of the environmental sample can indicate the existence of uranium irradiation,and even can be uesd to estimate the burnup of nuclear spent fuel.A study on the burnup estimation of irradiated uranium which was mixed with natural uranium was carried out by measuring the U/U and U/U in the samples by using MC-ICP-MS. According to the equation(1) and(2),a programme for calculating the burnup of the irradiated

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Journal of Microbiology and Immunology 2007年第2期5卷 116-120页

作者： yan jiang zhi QIN TANG LIN PENG YU LIANG WANG zhi PING WANG Central Laboratory Tianjin First Central Hospital Tianjin P. R. China

To investigate the imbalance state of helper T lymphocytes （Th） and cytotoxic T lymphocytes （Tc） and the roles of Thl/Th2/Th3 and Tcl/Tc2 cells in renal transplantation rejection, the percentages of these cells in peripheral blood of 24 cases of renal transplantation recipients with acute rejection and the dynamic changes of the CD4/CD8 ratio were determined by flow cytometry analysis, while 30 cases of healthy individuals were set up as controls. In these healthy controls, the percentages of the Thl, Th2 and Th3 cells were （ 10.45 ± 8.15） %, （5.05 ± 4.15） % and （3.90 ± 3.21 ） %, and those of Tcl and %2 cells were （9.83 ± 7.03） % and （4.51 ± 2.17） %, respectively. However, the percentages of Thl and Tcl cells in peripheral blood of the stable recipients after transplantation were （7.29 ± 5.62） % and （7.04 ± 5.15）%, showing definite reduction, while those of Th2, Th3 and Tc2 cells showed significant increase, （ 6.34 ± 5.67） %, （4.94 ± 4.14） % and （ 6.86 ± 4.42） %, respectively. In case of recipients with acute rejection, the percentages of Thl and Tcl cells appeared to be （ 18.55 ± 13.21 ） % and （ 15.84 ± 11.72） %, also showing significant increase, but those of Th2, Th3 and %2 cells appeared to be reduced, （4.19 ± 3.62） %, （3.02 ± 2.83 ） % and （3.88 ± 1.63） %, respectively. Significant differences could be detected among these three groups （ P 〈 0.05）. The CD4/CD8 ratio in cases with acute rejection was higher than those of stable recipients （2.24 ± 0.59 vs 1.95 ± 0.45）, but that of the stable recipients and healthy controls （ 1.98 ± 0.31 ） showed no any significant difference. From the above observation, it is evident that imbalance between Thl, Th2 and Th3 with Tcl and Tc2 cells may exist after renal transplantation and probably, the im- mune imbalance may be induced through the secretion of cytokines INF-γby Thl or Tcl cells , Ⅱ-4 by Th2 and Tc2 cells and TGF-β by Th3.

关键词： Th cells Tc cells Renal transplantation Acute rejection Cytometry

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Studies on DNA-protein interactions in the upstream regulatory region of the human ε-globin gene promoter

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Cell Research 1996年第2期6卷 101-114页

作者： yan zhi jiang YA DI CHEN RUO LAN QIAN (Shanghai Institute of Cell Biology, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China)( To whom correspondence should be addressed) Shanghai Institute of Cell Biology Chinese Academy of Sciences Shanghai China

The erythroid- and developmental stage-specific expression of the human ε-globin gene is controlled, in part,by the 5'-flanking DNA sequence of this gene. In the present study, we have used DNA-protein binding assays to identify trans-acting factors which regulate the temporal expression of the human ε-globin gene during development. Using gel mobility shift assays and DNasel footprinting assays, a nuclear protein factor (termed ε-SSF1) in the nuclear extracts from mouse haematopoietic tissues at d 11 and d 13 of gestation was identified. It could specifically bind to the positive control region (between -535 and -453bp) of the human ε-globin gene. We speculated that the E-SSF1 might be an erythroid- and developmental stage-specific activator. In addition, we found another nuclear protein factor (termed ε-R1) in the nuclear extract from mouse fetal liver at d 18 of gestation, which could strongly bind to the silencer region (between -392 and -177bp) of this gene. Therefore, we speculated that the ε-R1 might be an erythroid- and developmental stagespecific repressor. Our data suggest that both ε-SSF1 and ε-R1 might play important roles in developmental regulation of the human ε-globin gene expression during the early embryonic life. On the other hand, we observed that the binding patterns of nuclear proteins from three cell lines (K562, HEL and Raji) to these regulatory regions were partially different. These results suggest that different trans-acting factors in K562, HEL and Raji cells might be responsible for activating or silencing the human ε-globin gene in three different cell lines.

关键词： Human ε-globin gene positive control region silencer trans-acting factor

中等后掠角三角翼前缘双涡结构的形成机理数值研究

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空气动力学学报 2012年第6期30卷 767-771页

作者：刘杰刘沛清闫指江北京航空航天大学流体力学教育部重点实验室北京100191 成都飞机设计研究所四川成都610091

采用数值计算的方法,对前缘后掠角为50°的三角翼布局在不同迎角下的绕流结构及其演化进行了研究。在不同迎角下,计算的压力分布与实验结果吻合较好。对数值计算的流场结构分析发现:对于中等后掠角三角翼,在一定的雷诺数和迎角下,在机... 详细信息

采用数值计算的方法,对前缘后掠角为50°的三角翼布局在不同迎角下的绕流结构及其演化进行了研究。在不同迎角下,计算的压力分布与实验结果吻合较好。对数值计算的流场结构分析发现:对于中等后掠角三角翼,在一定的雷诺数和迎角下,在机翼翼面上会出现前缘双涡结构。其形成取决于三角翼前缘附近三维分离产生的自由剪切层强度和涡轴方向的速度分量之间的大小关系。一般而言,当涡轴的速度大到足以将剪切层涡量带走时,在机翼前缘常常会表现为单涡结构;反之将导致剪切层分叉,由此形成双涡结构。

关键词：双涡结构三角翼旋涡流场

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Zwitterionic hyaluronic acid derivatives for co-delivery of both chemotherapeutic and nucleic acid drugs in breast cancer treatment

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Nano Research 2025年第1期18卷 607-617页

作者： Ruo-Lin jiang Hui-Na Liu Yu-Fan yang zhi-Cheng Zhang Qi Dai Xiao-yan Bao Lin-Jie Wu Ya-Xin Qin Xin Tan Xiao-yan Sun Xu-Fang Ying zhi-Qing Ben Min Han Institute of Pharmaceutics College of Pharmaceutical SciencesZhejiang UniversityHangzhou 310058China Department of Radiation Oncology Key Laboratory of Cancer Prevention and InterventionThe Second Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhou 310058China Hangzhou Institute of Innovative Medicine Zhejiang UniversityHangzhou 310058China Jinhua Institute of Zhejiang University Jinhua 321299China National Key Laboratory of Advanced Drug Delivery and Release Systems Zhejiang UniversityHangzhou 310058China Polytechnic institute Zhejiang UniversityHangzhou 310015China

Traditionally,hyaluronic acid has been widely used for drug delivery,but the current application bottleneck is that hyaluronic acid is hydrophilic and electronegative,which makes it difficult to carry hydrophobic drugs and small interfering RNA(siRNA)with the same *** on previous studies,we designed and synthesized hyaluronic acid nanocarriers HA-spermine/N,N,N-trimethylcystamine/DOX-TPP(HSTD)for loading siRNA to overcome the problem of siRNA release caused by strong electrostatic ***,N,N,Ntrimethylcystamine in the carrier can be degraded by intracellular glutathione to completely and rapidly release siRNA,thus promoting ***,when codelivered with the chemotherapy drug doxorubicin(DOX),this novel nanocarrier showed promising synergy in inhibiting tumor growth.

关键词： zwitterionic hyaluronic acid N N N-trimethylcystamine small interfering RNA(siRNA) doxorubicin

Surface micro-arrays enhancing electromagnetic interference shielding of polydimethylsiloxane/multiwalled carbon nanotubes nanocomposites

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Nano Research 2025年第1期18卷 508-519页

作者： Ke jiang zhi Chen Ji Yu Chen Zheng Shan Huang Ming Wang Chongqing Key Laboratory of Soft-Matter Material Manufacturing School of Chemistry and Chemical EngineeringSouthwest UniversityChongqing 400715China School of Mechanics Civil Engineering and ArchitecturesNorthwestern Polytechnical UniversityXi’an 710072China Jieyang Branch of Chemistry and Chemical Engineering Guangdong Laboratory(Rongjiang Laboratory) Jieyang 515200China

Herein,the electromagnetic shielding performance of surface concave-convex(SC)and zig-zag micro-arrays was studied by using a simulation prediction and a three-dimensional(3D)printing custom ***,surface stripe concaveconvex(SSC)and surface cylindrical concave-convex(SCC)micro-arrays with or without zig-zag micro-arrays are designed,and their shielding performance is simulated in multi-bands(C-,X-band).The multiwalled carbon nanotubes/polydimethylsiloxane composites(MWCNT/PDMS)with different SC structures and different electrical conductivity are molded in acrylonitrile-butadiene-styrene copolymer(ABS)molds which are printed by a 3D *** results show that the electromagnetic interference shielding effectiveness(EMI SE)of the samples can be enhanced by constructing the SC micro-arrays with zig-zag micro-arrays,and improving with the increase of conductivity and *** addition,the shielding mechanism of the SC-MWCNT/PDMS composites is investigated and discussed by an electromagnetic simulation.

关键词： surface micro-arrays electrical properties electromagnetic interference(EMI)shielding three-dimensional(3D)printing

Frequency of cyclic strain modulate phenotype of smooth muscle cells associated with activation of p38

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Frequency of cyclic strain modulate phenotype of smooth musc...