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Action Mechanism Research of Lanthanons to Slow Vacuolar Ion Channels in Raphanus Satirus L.(Xinlimei) Radish by Patch-Clamp

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Chinese Chemical Letters 2001年第9期12卷 813-814页

作者： Pin yanG yan fang SHANG Zhen Ming PEI Institute of Molecular Science Shanxi UniversityTaiyuan 030006 Department of Biology Duke UniversityDurhamNC 27708

We used whole-vacuolar patch-clamp recording mode to study the action mechanism of La3+ to Slow Vacuolar (SV) channels for the first time. We recorded SV channel currents of Xinlimei (Raphanus satirus L.) vacuolars. The minimum activation potentials of voltage-dependent SV channels tied in 25+/-5 mV. The increase in cytoplasmic Ca2+ led to enhancement of SV-type currents. It was found that the threshold potential of activation shifted towards more depolarized values whenever cytoplasmic Ca2+ was increased. When 10(-10) mol/L free La3+ was added to the bath, SV-type current was suppressed by 60 similar to 75%. These data showed La3+ reduced ion permeabilities of Xinlimei root vacuolar membrane.

关键词： Whole-vacuolar recording SV-type current cytoplasmic Ca2+ La3+ Patch-Clamp

Biomimetic Fabrication of Polymer Film with High Adhesive Superhydrophobicity by Duplicating Locust Wing Surface

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Chemical Research in Chinese Universities 2015年第5期31卷 895-898页

作者： SUN Gang fang yan ZHI Heng LI Zhengwen School of Life Science Changchun Normal University Changchun 130032 P. R. China School of Life Science Northeast Normal University Changchun 130024 P. R. China

Locust is a common flying insect. Locust wings were used as biomimetic templates to fabricate multi-functional polymer（polydimethylsiloxane, PDMS） films by soft lithography. The microstructure and wettability of the natural and artificial locust wing surfaces were investigated by means of a scanning electron microscope（SEM） and a video-based contact angle meter. The natural locust wing surface exhibits complicated hierarchical structures and high adhesive superhydrophobicity（contact angle 152°）. The prepared polymer film faithfully reproduces the surface microstructures of the bio-template, and displays a good hydrophobicity and high adhesion（contact angle 144°）. The complex wettability of the natural and artificial locust wing surfaces ascribes to the cooperative effect of hydrophobic composition and multi-dimensional rough microstructures. This work not only promotes our understanding of the wetting mechanism on bio-surfaces, but offers an inexpensive and effective approach for biomimetic fabrication of multi-functional interfacial materials.

关键词： Biomimetic Superhydrophobicity Adhesion Polymer film Locust wing

QCM and EC-SPR Studies of Cytochrome c Self-assembled on Au Electrode and Enhancement of SPR Signal by Au Nanoparticles

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Chemical Research in Chinese Universities 2012年第6期28卷 1061-1065页

作者： WANG yan-yan JIANG yan-xla ZHOU You-chen LI yan-yan MA Zhi-fang SUN Shi-gang State Key Laboratory of Physical Chemistry of Solid Surfaces Department of ChemistryCollege of Chemistry and Chemical Engineering Xiamen University Xiamen 361005 P. R. China

Quartz crystal microbalance（QCM） and cyclic voltammetry（CV） were used to characterize the monolayer of cytochrome c（Cyt c）,which was adsorbed on gold film modified with alkanethiol mixed monolayer.A direct comparison of protein surface coverages calculated from QCM and cyclic voltammetric measurements illustrates that the ratio of the electroactive Cyt c to the total surface-confined Cyt c is 34%,which suggests that the orientation is a main factor affecting the electroactivity of Cyt ***,surface plasmon resonance（SPR） measurement combined with CV ＂in situ＂ was used to investigate the conformational change of Cyt c in the redox ***,Au nanoparticles（Au NPs） were adsorbed on the surface of Cyt *** result indicates that Au NPs promote electron transfer between Cyt c and the gold electrode,and SPR result suggests Au NPs enhance SPR signal.

关键词： Cytochrome c Gold film Self-assembly Electrochemical-surface plasmon resonance(EC-SPR)

Study of the coordinative nature of alkylaluminum modified Phillips CrO_x/SiO_2 catalyst by multinuclear solid-state NMR

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Petroleum Science 2013年第4期10卷 577-583页

作者： Zhang Qin-Hui yan fang Xia Wei Liu Chenguang State Key Laboratory of Heavy Oil Processing College of Chemical Engineering China University of Petroleum

Solid-state nuclear magnetic resonance spectroscopy was used to investigate the coordinative states of surface Al species on various alkylaluminum-modified Phillips CrOx/SiO2 *** alkylaluminum-modified Phillips CrOx/SiO2 catalysts were examined via ethylene homopolymerization.1H and 27Al magic angle spinning（MAS） nuclear magnetic resonance（NMR） spectra clearly demonstrated that the existing states of surface Al species in alkylaluminum-modified catalysts strongly depended on the type of alkylaluminum cocatalyst,concentration of alkylaluminum and the calcination temperature.1H MAS NMR spectra of alkylaluminum-modified Phillips CrOx/SiO2 catalysts,calcined at two different temperatures,exhibited similar trends in peak shift.1H spectra showed that with an increase of Al/Cr ratio and calcination temperature,the main peak shifted to high field,indicating that the dominant surface proton species changed from hydroxyl to ethoxyl and ethyl groups.27Al MAS NMR spectra showed the presence of three different coordination states（6-,5-,and 4-coordinated Al species） in the alkylaluminummodified Phillips *** comparison of different alkylaluminum cocatalysts,it was found that the reactivity of alkylaluminum modified Phillips catalyst decreased in the order of TEA〉DEAH〉*** amount of 4-coordinated Al species of Phillips catalysts modified by TEA,DEAE and DEAH also decreased in the order of TEA〉DEAH〉DEAE,indicating that the presence of 4-coordinated Al species is related to the polymerization activity.

关键词： Phillips catalyst alkylaluminum ethylene polymerization solid-state NMR

Gene engineering in swine for agriculture

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Journal of Integrative Agriculture 2017年第12期16卷 2792-2804页

作者： WANG yan-fang HUANG Jiao-jiao ZHAO Jian-guo Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing 100193 P.R. China Institute of Zoology Chinese Academy of Sciences Beijing 100101 P.R.China University of Chinese Academy of Sciences Beijing 100049 P.R. China

Domestic pigs are the second most important source of meat world-wide, and the genetic improvement of economic traits, such as meat production, growth, and disease resistance, is a critical point for efficient production in pigs. Through conventional breeding and selection programs in pigs, which are painstakingly slow processes, some economic traits, such as growth and backfat, have been greatly improved over the past several decades. However, the improvement of many polygenetic traits is still very slow and challenging to be improved by conventional breeding strategies. The development of reproductive knowledge and a variety of techniques, including foreign gene transfer strategies, somatic cell nuclear transfer（SCNT） and particularly, recently developed nuclease-mediated genome editing tools, has provided efficient ways to produce genetically modified（GM） pigs for the dramatic improvement of economic traits. In this review, we briefly discuss the progress of genomic markers used in pig breeding program, trace the history of genetic engineering, mainly focusing on the progress of recently developed genome editing tools, and summarize the GM pigs which have been generated to aim at the agricultural purposes. We also discuss the specific challenges facing application of gene engineering in pig breeding, and future prospects.

关键词： gene engineering genome editing pig agricultural application

Infinitely Many Periodic Solutions for a Class of Second-order Hamiltonian Systems

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Acta Mathematicae Applicatae Sinica 2016年第1期32卷 231-238页

作者： Ming-hai yanG Yue-fen CHEN yan-fang XUE Department of Mathematics Xinyang Normal University

In this paper we study the existence of infinitely many periodic solutions for second-order Hamiltonian systems{ü（t）＋A（t）u（t）＋▽F（t,u（t））=0,u（0）-u（T）=u^·（0）-u^·（T）=0,where F（t,u） is even in u,... 详细信息

关键词： second-order Hamiltonian systems periodic solutions Fountain theorem

A Linear Perturbed Palais-Smale Condition for Lower Semicontinuous Functions on Banach Spaces

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Acta Mathematica Sinica,English Series 2008年第11期24卷 1853-1860页

作者： Li Xing CHENG Xiao yan LIU Mai fang ZUO Department of Mathematics Xiamen University Xiamen 361005 P. R. China

This paper introduces a notion of linear perturbed Palais-Smale condition for real-valued functions on Banach spaces. In terms of strongly exposed points, it presents a characterization which guarantees linear perturbed Palais-Smale condition holds for lower semicontinuous functions with bounded effective domains defined on a Banach space with the Radon-Nikody＇m property; and gives an example showing that linear perturbed P-S condition is strictly weaker than the P-S condition.

关键词： Palais-Smale condition variational question perturbed optimization Radon-Nikody'm property Banach space

Modeling of hydraulic turbine systems based on a Bayesian-Gaussian neural network driven by sliding window data

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Journal of Zhejiang University-Science C(Computers and Electronics) 2010年第1期11卷 56-62页

作者： Yi-jian LIU yan-jun fang Xue-mei ZHU School of Electric & Automation Engineering Nanjing Normal University Nanjing 210042 China Department of Automation Wuhan University Wuhan 430072 China

In this paper, a novel Bayesian-Gaussian neural network (BGNN) is proposed and applied to on-line modeling of a hydraulic turbine system (HTS). The new BGNN takes account of the complex nonlinear characteristics of HTS. Two redefined training procedures of the BGNN include the off-line training of the threshold matrix parameters, optimized by swarm optimiza- tion algorithms, and the on-line BGNN predictive application driven by the sliding window data method. The characteristics models of an HTS are identified using the new BGNN method and simulation results are presented which show the effectiveness of the BGNN in addressing modeling problems of HTS.

关键词： Bayesian-Gaussian neural network (BGNN) Hydraulic turbine Modeling Sliding window data

Two-dimensional MX Dirac materials and quantum spin Hall insulators with tunable electronic and topological properties

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Nano Research 2021年第3期14卷 584-589页

作者： yan-fang Zhang Jinbo Pan Huta Banjade Jie Yu Hsin Lin Arun Bansil Shixuan Du Qimin yan Institute of Physics&University of Chinese Academy of Sciences Chinese Academy of SciencesBeijing100190China Department of Physics Temple UniversityPhiladelphiaPA19122USA Institute of Physics “Academia Sinica”Taipei11529Taiwan Physics Department Northeastern UniversityBostonMA02115USA

We propose a novel class of two-dimensional(2D)Dirac materials in the MX family(M=Be,Mg,Zn and Cd,X=Cl,Br and I),which exhibit graphene-like band structures with linearly-dispersing Dirac-cone states over large energy scales(0.8–1.8 eV)and ultra-high Fermi velocities comparable to ***-orbit coupling opens sizable topological band gaps so that these compounds can be effectively classified as quantum spin Hall *** electronic and topological properties are found to be highly tunable and amenable to modulation via anion-layer substitution and vertical electric *** structures of several members of the family are shown to host a Van-Hove singularity(VHS)close to the energy of the Dirac *** enhanced density-of-states associated with these VHSs could provide a mechanism for inducing topological *** presence of sizable band gaps,ultra-high carrier mobilities,and small effective masses makes the MX family promising for electronics and spintronics applications.

关键词： two-dimensional Dirac materials density functional theory topological properties