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Integrated analysis of miRNAs and transcriptomes in Aedes albopictus midgut reveals the differential expression profiles of immune-related genes during dengue virus serotype-2 infection

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Insect Science 2016年第3期23卷 377-385页

作者： yan-xia liu Fen-xiang Li Zhuan-Zhuan liu Zhi-Rong Jia yan-He Zhou Hao Zhang Hui yan xian-Qiang Zhou xiao-Guang Chen Key Laboratory of Prevention and Control of Emerging Infectious Diseases of Guangdong Higher Education Institutes Department ofPathogen Biology School of Public Health and Tropical Medicine Southern Medical University Guangzhou Laboratory of EmergingInfectious Diseases and Division of Laboratory Medicine Zhujiang Hospital Southern Medical University Guangzhou BGI-Shenzhen Shenzhen Guangdong Province China

Mosquito microRNAs （miRNAs） are involved in host-virus interaction, and have been reported to be altered by dengue virus （DENV） infection in Aedes albopictus （Diptera： Culicidae）. However, little is known about the molecular mechanisms of Aedes albopictus midgut--the first organ to interact with DENV--involved in its resistance to DENV. Here we used high-throughput sequencing to characterize miRNA and messenger RNA （mRNA） expression patterns in Aedes albopictus midgut in response to dengue virus serotype 2. A total of three miRNAs and 777 mRNAs were identified to be differentially expressed upon DENV infection. For the mRNAs, we identified 198 immune-related genes and 31 of them were differentially expressed. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses also showed that the differentially expressed immune-related genes were involved in immune response. Then the differential expression patterns of six immune-related genes and three miRNAs were confirmed by real-time reverse transcription polymerase chain reaction. Furthermore, seven known miRNA-mRNA interaction pairs were identified by aligning our two datasets. These analyses of miRNA and mRNA transcriptomes provide valuable information for uncovering the DENV response genes and provide a basis for future study of the resistance mechanisms in Aedes albopictus midgut.

关键词： Aedes albopictus DENV host-pathogen interaction midgut miRNA mRNA

Rice cultivation changes and its relationships with geographical factors in Heilongjiang Province,China

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Journal of Integrative Agriculture 2017年第10期16卷 2274-2282页

作者： LU Zhong-jun SONG Qian liu Ke-bao WU Wen-bin liu yan-xia XIN Rui ZHANG Dong-mei Remote Sensing Technology Center Heilongjiang Academy of Agricultural Sciences Key Laboratory of Agricultural Remote Sensing Ministry of Agriculture/Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural Sciences

Rice planting patterns have changed dramatically over the past several decades in northeast China （NEC） due to the combined influence of global change and agricultural policy. Except for its great implications for environmental protection and climate change adaption, the spatio-temporal changes of rice cultivation in NEC are not clear. In this study, we conducted spatio-temporal analyses of NEC＇s major rice production region, Heilongjiang Province, by using satellite-derived rice cultivation maps. We found that the total cultivated area of rice in Heilongjiang Province increased largely from 1993 to 2011 and it expanded spatially to the northern and eastern part of the Sanjiang Plain. The results also showed that rice cultivation areas experienced a larger increase in the region managed by the Reclamation Management Bureau （RMB） than that managed by the local provincial government. Rice cultivation changes were closely related with those geographic factors over the investigated periods, represented by the geomorphic （slope）, climatic （accumulated temperature）, and hydrological （watershed） variables. These findings provide clear evidence that crop cultivation in NEC has been modified to better cope with the global change.

关键词： paddy rice spatio-temporal change cultivation area geographical factors

Protective effect of tea polyphenols against paracetamol-induced hepatotoxicity in mice is significanly correlated with cytochrome P450 suppression

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World Journal of Gastroenterology 2009年第15期15卷 1829-1835页

作者： xia Chen Chang-Kai Sun Guo-Zhu Han Jin-Yong Peng Ying Li yan-xia liu Yuan-Yuan Lv Ke-Xin liu Qin Zhou Hui-Jun Sun Department of Pharmacology Daiian Medical University Dalian 116044 Liaoning Province China

AIM： To investigate the hepatoprotective activity of tea polyphenols （TP） and its relation with cytochrome P450 （CYP450） expression in mice. METHODS： Hepatic CYP450 and CYPbs levels were measured by UV-spectrophotometry in mice 2 d after intraperitoneal TP （25, 50 and 100 mg/kg per day）. Then the mice were intragastricly pre-treated with TP （100, 200 and 400 mg/kg per day） for six days before paracetamol （1000 mg/kg） was given. Their acute mortality was compared with that of control mice. The mice were pre-treated with TP （100, 200, and 400 mg/kg per day） for five days before paracetamol （500 mg/kg） was given. Hepatic CYP2E1 and CYPIA2 protein and mRNA expression levels were evaluated by Western blotting, immunohistochemical staining and transcriptase-polymerase chain reaction. RESULTS： The hepatic CYP450 and CYPb5 levels in mice of TP-treated groups （100, 200 and 400 mg/kg per day） were decreased in a dose-dependent manner compared with those in the negative control *** significantly attenuated the paracetamol-induced hepatic injury and dramatically reduced the mortality of paracetamol-treated mice. Furthermore, TP reduced CYP2E1 and CYPIA2 expression at both protein and mRNA levels in a dose-dependent manner. CONCLUSION： TP possess potential hepatoprotective properties and can suppress CYP450 expression.

关键词： Tea polyphenols Cytochrome P450 Paracetamol-induced hepatotoxicity

Effects of endothelial microvesicles induced by A23187 on H9c2 cardiomyocytes

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中国应用生理学杂志 2013年第6期29卷 559-564页

作者： Man SHANG Qi ZHANG Meng-xiao ZHANG Yao WANG yan CHEN yan-na WU Jun-qiuSONG Ming-lin liu yan-xia liu Department of pharmacology School of Basic Medical Sciences Tianjin Medical University Tianjin 300070 China Section of Endocrinology Department of Medicine Temple University School of Medicine Philadelphia 19140USA

Objective To investigate the effects of endothelial microvesicles(EMVs) induced by calcium ionophore A23187 on H9c2 cardiomyocytes. Methods Human umbilical vein endothelial cells(HUVECs) were treated with 10 μmol/L A23187 for 30 min. EMVs from HUVECs were isolated by ultracentrifugation from the conditioned culture medium. EMVs were characterized using 1 and 2 μm latex beads and antiPE-CD144 antibody by flow cytometry. For functional research, EMVs at different concentrations were cocultured with H9c2 cardiomyocytes for 6 h. Cell viability of H9c2 cells and the activity of LDH leaked from H9c2 cells were tested by colorimetry. Moreover, apoptosis of H9c2 cells was observed through Hoechst 33258 staining and tested by FITC-Annexin V/PI double staining. Results EMVs were induced by A23187 on HUVECs, and isolated by ultracentrifugation. We identified the membrane vesicles(< 1 μm) induced by A23187 were CD144 positive. In addition, the EMVs could significantly reduce the viability of H9c2 cells, and increase LDH leakage from H9c2 cells in a dose dependent manner(P<0.05). Condensed nuclei could be observed with the increasing concentrations of EMVs through Hoechst 33258 staining. Furthermore, increased apoptosis rates of H9c2 cells could be assessed through FITC-Annexin V/PI double staining by flow cytometry. Conclusion Microvesicles could be released from HUVECs after induced by A23187 through calcium influx, and these EMVs exerted a pro-apoptotic effect on H9c2 cells by induction of apoptosis.

关键词：人脐静脉内皮细胞心肌细胞凋亡诱导微泡流式细胞术检测钙离子载体膜联蛋白V 荧光染色

Effects of coke charging pattern on burden movement in COREX melter gasifier based on physical simulation

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Journal of Iron and Steel Research International 2022年第6期29卷 907-913页

作者： Li-hao Han Yue-hua Li Li-ning Han yan-xia liu Wei-qing Huang Lei Cao Su-ci Qi Xin Guan Jian-lu Yuan Department of Material Engineering Hebei College of Industry and TechnologyShijiazhuang 050091HebeiChina Department of Basic Course Teaching Hebei College of Industry and TechnologyShijiazhuang 050091HebeiChina First Rolling Mill Anyang Iron&Steel Co.Ltd.Anyang 455000HenanChina

A three-dimensional semicircle cold model of COREX melter gasifier(MG)was established,and the effects of coke charging pattern on burden movement were investigated by adopting this *** burden flow pattern of packed bed was measured with different coke charging amounts,coke charging positions,and coke *** results show that the solid flow pattern presents a change from straight line type to reverse U type and finally to W type in MG with traditional coke charging,while the burden flow pattern experiences the change from straight line type to W type with platform and finally W type under center coke charging,and the solid flow pattern presents a change from straight line type to W type with platform and finally W type under intermediate coke *** difference demonstrates that coke charging pattern affects the uniform descending in MG to some *** burden in the coke charging zone keeps a straight line *** matter what kind of coke charging pattern is adopted,the burden residence time is shortened with the increase in coke charging amount,and the vertex of deadman decreases obviously,which is conducive to the renewal of the *** appropriate coke charging amount and coke size should be well controlled at 8.7%and 5 mm,*** results are useful not only in developing further understanding of solid flow in MG but also in verifying different mathematical models,particularly the coupled discrete element method with computational fluid dynamics simulation which has been increasingly used in the literature.

关键词： COREX melter gasifier Coke charging pattern Burden movement Flow pattern Physical simulation

Microvesicles derived from hypoxia/reoxYgenation-treated human umbilical vein endothellal cells impair relaxation of rat thoracic aortic rings

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中国应用生理学杂志 2014年第6期30卷 560-566页

作者： Shao-xun WANG Qi ZHANG Man SHANG Su WEI Miao liu Yi-lu WANG Meng-xiao ZHANG yan-na WU Ming-lin liu Jun-qiu SONG yan-xia liu Department of Pharmacology School of Basic Medical SciencesTianjin Medical University Section of Endocrinology Department of MedicineTemple University School of Medicine Department of Dermatology Perelman School of MedicineUniversity of Pennsylvania

Objective To investigate the effects of microvesicles(MVs) derived from hypoxia/reoxygenation(H/R)-treated human umbilical vein endothelial cells(HUVECs) on endothelium-dependent relaxation of rat thoracic aortic *** H/R injury model was established to induce HUVECs to release H/R-EMVs.H/R-EMVs from HUVECs were isolated by ultracentrifugation from the conditioned culture medium.H/R-EMVs were characterized using 1 urn latex beads and anti-PE-CD144 by flow *** aortic rings of rats were incubated with 2.5,5,10,20 μg/ml H/R-EMVs derived from H/R-treated HUVECs for 4 hours,and their endothelium-dependent relaxation in response to acetylcholine(ACh) or endothelium-independent relaxation in response to sodium nitroprusside(SNP) was recorded in *** nitric oxide(NO) production of ACh-treated thoracic aortic rings of rats was measured using Griess *** expression of endothelial NO synthase(eNOS) and phosphorylated eNOS(p-eNOS,Ser-1177) in the thoracic aortic rings of rats was detected by Western ***,the levels of SOD and MDA in H/R-EMVs-treated thoracic aortic rings of rats were measured using SOD and MDA *** H/R-EMVs were induced by H/R-treated HUVECs and isolated by *** membrane vesicles(lium-dependent relaxation of thoracic aortic rings of rats was impaired by H/R-EMVs in a concentration-dependent *** mechanisms included a decrease in NO production,p-eNOS expression and an increase in oxidative stress.

关键词：人脐静脉内皮细胞主动脉大鼠细胞来源微泡复氧缺氧浓度依赖性

BMI, WC, WHtR, VFI and BFI: Which Indictor is the Most Efficient Screening Index on Type 2 Diabetes in Chinese Community Population

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Biomedical and Environmental Sciences 2013年第6期26卷 485-491页

作者： MI Sheng Quan YIN Peng HU Nan LI Jian Hong CHEN xiao Rong CHEN Bo yan liu xia ZHAO Wen Hua College of Arts and Science of Beijing Union University National Center for Chronic and Non-communicable Disease Control and Prevention of China CDC Chinese Center for Disease Control and Prevention

Objective Obesity is a major risk factor for type 2 diabetes, many indexes can be used to describes obesity and predict diabetes. This research attempts to identify the best indicator of obesity to screening diabetes in Chinese population. Methods A cross-sectional data of 8121 subjects aged 35-60 years were included in this research belongs to the Diabetes Appropriate Technology Intervention Study. Anthropometric indicators including body weight, height, waist circumferences （WC）, body fat index （BFI） and visceral fat index （VFI） and blood biochemical indicators after an overnight fast [fasting blood glucose, total cholesterol, high-density lipoprotein （HDL） cholesterol, and triacylglycerol] were measured. BMI （body mass index） and Weight to Height Ratio was calculated. Results Subjects with obesity had a higher risk of physician diagnosed diabetes （0R=2.50, 95% CI 1.83-3.43）, new diagnosed diabetes （0R=4.23, 95% Cl 2.91-6.15） and pre-diabetes （0R=1.75, 95% CI 1.31-2.34） compared to those with normal Body mass index （BMI）. There was a significant trend of increased risk of all diabetes status with increased waist circumference （WC）. The waist-to-height ratio （WHtR） yielded the most significant association with new diagnosed diabetes and physician diagnosed diabetes than other indices. Conclusion Central obesity is significantly correlated with diabetes. VFI was most correlated with pre-diabetes while WHtR is an efficient screening index than BMI and WC in Chinese community diabetes screening.

关键词： Obesity Diabetes BMI Waist Circumference Waist-to-height ratio

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Ti_(2)AlNb合金的嵌入原子势

物理学报 2022年第20期71卷 65-74页

作者：刘杰刘艳侠辽宁大学物理学院沈阳110036

分子动力学模拟是一种行之有效的计算机模拟方法;然而,由于缺少合适的多元合金原子间势,因而限制了分子动力学模拟的应用.多元合金原子间势的开发一直具有挑战性.本文在嵌入原子势模型的框架下,提出一种适用于三元有序合金的原子间势构... 详细信息

分子动力学模拟是一种行之有效的计算机模拟方法;然而,由于缺少合适的多元合金原子间势,因而限制了分子动力学模拟的应用.多元合金原子间势的开发一直具有挑战性.本文在嵌入原子势模型的框架下,提出一种适用于三元有序合金的原子间势构建方法,并开发了适用于原子尺度力学行为模拟的Ti_(2)AlNb合金新型原子间势.该势能够很好地再现B2-Ti_(2)AlNb的弹性常数、未弛豫的空位形成能、置换原子形成能、换位原子形成能、表面能和三种有序构型(B2相、D0_(19)相、O相)在不同体积下的内聚能.为了进一步检验势函数,计算了B2相的E-V曲线,结果与Rose曲线符合得很好;利用分子动力学模拟研究了B2相的熔化转变过程,结果大致反映了实验情况.本文的工作一方面为开发多元合金原子间势提供一种途径,另一方面为模拟计算Ti_(2)AlNb合金的工作者提供一种选择.

关键词：嵌入原子势 Ti_(2)AlNb合金缺陷

Flow cytometric analysis of circulating microvesicles derived from myocardial ischemic preconditioning and cardioprotection of ischemia/reperfusion injury in rats

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中国应用生理学杂志 2015年第6期31卷 524-531页

作者： Miao liu Yi-lu WANG Man SHANG Yao WANG Qi ZHANG Shao-xun WANG Su WEI Kun-wei ZHANG Chao liu yan-na WU Ming-lin liu Jun-qiu SONG yan-xia liu Department of Pharmacology School of Basic Medical Sciences Tianjin Medical University Section of Endocrinology Department of Medicine Temple University School of Medicine Department of Dermatology Perelman School of Medicine University of Pennsylvania

Objective: To establish a flow cytometric method to detect the alteration of phenotypes and concentration of circulating microvesicles(MVs) from myocardial ischemic preconditioning(IPC) treated rats(IPC-MVs), and to investigate the effects of IPC-MVs on ischemia/reperfusion(I/R) injury in rats. Methods: Myocardial IPC was elicited by three cycles of 5-min ischemia and 5-min reperfusion of the left anterior descending(LAD) coronary artery. Platelet-free plasma(PFP) was isolated through two steps of centrifugation at room temperature from the peripheral blood, and IPC-MVs were isolated by ultracentrifugation from PFP. PFP was incubated with anti-CD61, anti-CD144, anti-CD45 and anti-Erythroid Cells, and added 1, 2 μm latex beads to calibrate and absolutely count by flow cytometry. For functional research, I/R injury was induced by 30-min ischemia and 120-min reperfusion of LAD. IPC-MVs 7 mg/kg were infused via the femoral vein in myocardial I/R injured rats. Mean arterial blood pressure(MAP), heart rate(HR) and ST-segment of electrocardiogram(ECG) were monitored throughout the experiment. Changes of myocardial morphology were observed after hematoxylin-eosin(HE) staining. The activity of plasma lactate dehydrogenase(LDH) was tested by Microplate Reader. Myocardial infarct size was measured by TTC staining. Results: Total IPC-MVs and different phenotypes, including platelet-derived MVs(PMVs), endothelial cell-derived MVs(EMVs), leucocyte-derived MVs(LMVs) and erythrocyte-derived MVs(RMVs) were all isolated which were identified membrane vesicles(<1 μm) with corresponding antibody positive. The numbers of PMVs, EMVs and RMVs were significantly increased in circulation of IPC treated rats(P<0.05, respectively). In addition, at the end of 120-min reperfusion in I/R injured rats, IPC-MVs markedly increased HR(P<0.01), decreased ST-segment and LDH activity(P<0.05, P<0.01). The damage of myocardium was obviously alleviated and myocardial infarct size was significantly lowered

关键词：缺血/再灌注损伤流式细胞仪分析心肌梗死缺血预处理保护作用大鼠循环微泡